Click here to switch to the map view.

The map label for this gene is bioF

Identifier: 115353032

GI number: 115353032

Start: 3290071

End: 3291486

Strand: Direct

Name: bioF

Synonym: Bamb_2981

Alternate gene names: 115353032

Gene position: 3290071-3291486 (Clockwise)

Preceding gene: 115353031

Following gene: 115353033

Centisome position: 92.51

GC content: 71.82

Gene sequence:

>1416_bases
ATGACACAAACGGCCCCAAGTTCACTTCGTTCGCTGCCCCCCGAGGGGGCGGCAACGACGAACCTGCTCGATACGCTGCA
ACGCGGCCTCGCCGAGCTCGACGCACAAGGGTTGCGCCGCGTGCGCCGCACCGCCGACACCGCGTGCGATGCGCACATGC
GCGTCGACGGCCGCGACATCGTCGGCTTCGCGAGTAACGACTATCTCGGCCTCGCCGCGCACCCGGCGCTCGTCGCCGCG
TTCGCCGAAGGCGCGCGGCGCTACGGCTCCGGCAGCGGCGGCTCGCATCTGCTCGGCGGCCATTCGCGCGCGCATGCCAC
GCTCGAGGACGAACTCGCCGGCTTCGCGGGCGGCTTCTCCGATGCGCCGCGCGCGCTGTACTTCAGCACCGGCTACATGG
CGAACCTCGCCGCGATGACGGCGCTCACCGGCAAACACGCGACGATCTTCTCCGATGCGCTGAACCACGCGTCGCTGATC
GACGGCATGCGGCTGTCGCGCGCGAACGTGCAGGTCTACCCGCATGCGGACATGGCCGCGCTCGCCGCGCTGCTCGACGC
GTCCGACGCCGAAACGAAACTGATCGTCAGCGACACCGTGTTCAGCATGGATGGCGACATCGCGCCGCTCGCCGAACTCG
TCGCGCTGGCCGAACGCCACGGCGCGTGGCTCGTCGTCGACGACGCGCACGGCTTCGGCGTGCTGGGGCCCCAGGGCCGC
GGCGCGCTCGCGGCCGCCGCGCTGCGCTCGCCGAATCTCATCTACGTCGGCACGCTCGGCAAGGCCGCCGGCGTCGCGGG
CGCGTTCGTGATCGCGCACGAGACCGTGATCGAATGGATGATCCAGCGCGCGCGCAGCTACATCTTCACGACGGCCGCGC
CGCCGGCCGTCGCGCATGCGGTATCGGCGAGCCTGAAGGTGATCGCCGGCGACGAAGGCGACGCGCGACGCGCGCATCTC
GCCGCGCTGATCGAACGCACGCGCGCGCTGCTGCGCATGACGCGCTGGCAGCCGGTCGACTCGCACACTGCCGTGCAGCC
GCTCGTGATCGGCAGCAACGATGCGACGCTCGCGGCGATGCGCTCGCTCGACGCGCACGGCCTGTGGGTGCCCGCGATCC
GGCCGCCGACGGTGCCCGTCGGCACGTCGCGGCTGCGCGTATCGCTGTCGGCCGCGCATTCGTTCGACGATCTCGCACGG
CTCGAAGCAGCCTTGATCGAAGCCAGCGAGGGGCAGACCCGGCGGGAGGCAGAACAGCCCCCACGCTCACTGCGTTCGCT
GCCCCCCGAGGGGGCTGCAGTCTCCGTTGGGGCGGCCCGGCGGGATGCAGAACAACCCCCACGCTCACTGCGTTCGCTGC
CCCCCGAGGGGGCTGCAGCCTCCCTTGGGGCGGCCCGGCGGGAGACTGCGGCATGA

Upstream 100 bases:

>100_bases
TGTACCTGATGCCGCCGTACGTGATGAGCGACGACGACATCGCGTGGCTTGCGCAGCGCACGCGCGACACGCTGGATGCG
ACGCTCGCGGAGATCATGCA

Downstream 100 bases:

>100_bases
CCGCCCCGCTCTCGCTGTTCGTCACCGGCACCGACACCGAAATCGGCAAGACCTTCGTCTCGGCTGCGATGCTGCACGGC
TTCGCGCGGCATGGCCTGCG

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTQTAPSSLRSLPPEGAATTNLLDTLQRGLAELDAQGLRRVRRTADTACDAHMRVDGRDIVGFASNDYLGLAAHPALVAA
FAEGARRYGSGSGGSHLLGGHSRAHATLEDELAGFAGGFSDAPRALYFSTGYMANLAAMTALTGKHATIFSDALNHASLI
DGMRLSRANVQVYPHADMAALAALLDASDAETKLIVSDTVFSMDGDIAPLAELVALAERHGAWLVVDDAHGFGVLGPQGR
GALAAAALRSPNLIYVGTLGKAAGVAGAFVIAHETVIEWMIQRARSYIFTTAAPPAVAHAVSASLKVIAGDEGDARRAHL
AALIERTRALLRMTRWQPVDSHTAVQPLVIGSNDATLAAMRSLDAHGLWVPAIRPPTVPVGTSRLRVSLSAAHSFDDLAR
LEAALIEASEGQTRREAEQPPRSLRSLPPEGAAVSVGAARRDAEQPPRSLRSLPPEGAAASLGAARRETAA

Sequences:

>Translated_471_residues
MTQTAPSSLRSLPPEGAATTNLLDTLQRGLAELDAQGLRRVRRTADTACDAHMRVDGRDIVGFASNDYLGLAAHPALVAA
FAEGARRYGSGSGGSHLLGGHSRAHATLEDELAGFAGGFSDAPRALYFSTGYMANLAAMTALTGKHATIFSDALNHASLI
DGMRLSRANVQVYPHADMAALAALLDASDAETKLIVSDTVFSMDGDIAPLAELVALAERHGAWLVVDDAHGFGVLGPQGR
GALAAAALRSPNLIYVGTLGKAAGVAGAFVIAHETVIEWMIQRARSYIFTTAAPPAVAHAVSASLKVIAGDEGDARRAHL
AALIERTRALLRMTRWQPVDSHTAVQPLVIGSNDATLAAMRSLDAHGLWVPAIRPPTVPVGTSRLRVSLSAAHSFDDLAR
LEAALIEASEGQTRREAEQPPRSLRSLPPEGAAVSVGAARRDAEQPPRSLRSLPPEGAAASLGAARRETAA
>Mature_470_residues
TQTAPSSLRSLPPEGAATTNLLDTLQRGLAELDAQGLRRVRRTADTACDAHMRVDGRDIVGFASNDYLGLAAHPALVAAF
AEGARRYGSGSGGSHLLGGHSRAHATLEDELAGFAGGFSDAPRALYFSTGYMANLAAMTALTGKHATIFSDALNHASLID
GMRLSRANVQVYPHADMAALAALLDASDAETKLIVSDTVFSMDGDIAPLAELVALAERHGAWLVVDDAHGFGVLGPQGRG
ALAAAALRSPNLIYVGTLGKAAGVAGAFVIAHETVIEWMIQRARSYIFTTAAPPAVAHAVSASLKVIAGDEGDARRAHLA
ALIERTRALLRMTRWQPVDSHTAVQPLVIGSNDATLAAMRSLDAHGLWVPAIRPPTVPVGTSRLRVSLSAAHSFDDLARL
EAALIEASEGQTRREAEQPPRSLRSLPPEGAAVSVGAARRDAEQPPRSLRSLPPEGAAASLGAARRETAA

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=391, Percent_Identity=34.0153452685422, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI284448556, Length=364, Percent_Identity=34.0659340659341, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI83977442, Length=374, Percent_Identity=35.2941176470588, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI83977444, Length=374, Percent_Identity=35.2941176470588, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI83977440, Length=374, Percent_Identity=35.2941176470588, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI4502025, Length=344, Percent_Identity=31.9767441860465, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI40316939, Length=344, Percent_Identity=31.9767441860465, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4758668, Length=370, Percent_Identity=28.9189189189189, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI119220554, Length=378, Percent_Identity=27.2486772486773, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI5454084, Length=271, Percent_Identity=26.9372693726937, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1786993, Length=346, Percent_Identity=46.242774566474, Blast_Score=277, Evalue=9e-76,
Organism=Escherichia coli, GI1790046, Length=374, Percent_Identity=36.6310160427808, Blast_Score=203, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI71994529, Length=410, Percent_Identity=32.6829268292683, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI32566772, Length=374, Percent_Identity=27.5401069518717, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17560912, Length=364, Percent_Identity=26.9230769230769, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17560914, Length=364, Percent_Identity=26.9230769230769, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71982625, Length=286, Percent_Identity=25.1748251748252, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71982617, Length=286, Percent_Identity=25.1748251748252, Blast_Score=95, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6320438, Length=453, Percent_Identity=29.1390728476821, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6320267, Length=363, Percent_Identity=28.9256198347107, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6323954, Length=267, Percent_Identity=28.0898876404494, Blast_Score=101, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24662918, Length=400, Percent_Identity=33.25, Blast_Score=194, Evalue=7e-50,
Organism=Drosophila melanogaster, GI17137420, Length=359, Percent_Identity=30.9192200557103, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17136286, Length=357, Percent_Identity=27.1708683473389, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24653280, Length=276, Percent_Identity=26.4492753623188, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24653276, Length=276, Percent_Identity=26.4492753623188, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24653278, Length=276, Percent_Identity=26.4492753623188, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_BURCM (Q0BBD6)

Other databases:

- EMBL:   CP000440
- RefSeq:   YP_774871.1
- ProteinModelPortal:   Q0BBD6
- STRING:   Q0BBD6
- GeneID:   4310568
- GenomeReviews:   CP000440_GR
- KEGG:   bam:Bamb_2981
- NMPDR:   fig|339670.3.peg.183
- eggNOG:   COG0156
- HOGENOM:   HBG649839
- OMA:   KDYASND
- ProtClustDB:   PRK05958
- BioCyc:   BAMB339670:BAMB_2981-MONOMER
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 49159; Mature: 49028

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 40-40 BINDING 156-156 BINDING 202-202 BINDING 377-377

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTAPSSLRSLPPEGAATTNLLDTLQRGLAELDAQGLRRVRRTADTACDAHMRVDGRDI
CCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEE
VGFASNDYLGLAAHPALVAAFAEGARRYGSGSGGSHLLGGHSRAHATLEDELAGFAGGFS
EEECCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCHHHHHHHHHCCCCC
DAPRALYFSTGYMANLAAMTALTGKHATIFSDALNHASLIDGMRLSRANVQVYPHADMAA
CCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEECCCCEEEECCCHHHH
LAALLDASDAETKLIVSDTVFSMDGDIAPLAELVALAERHGAWLVVDDAHGFGVLGPQGR
HHHHHCCCCCCCEEEEECEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
GALAAAALRSPNLIYVGTLGKAAGVAGAFVIAHETVIEWMIQRARSYIFTTAAPPAVAHA
CHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHH
VSASLKVIAGDEGDARRAHLAALIERTRALLRMTRWQPVDSHTAVQPLVIGSNDATLAAM
HHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHH
RSLDAHGLWVPAIRPPTVPVGTSRLRVSLSAAHSFDDLARLEAALIEASEGQTRREAEQP
HHCCCCCEEECCCCCCCCCCCCHHEEEEEHHHCCHHHHHHHHHHHHHCCCCCHHHHHHCC
PRSLRSLPPEGAAVSVGAARRDAEQPPRSLRSLPPEGAAASLGAARRETAA
CHHHHCCCCCCCEEECCHHHCCCCCCCHHHHCCCCCCCHHHHCCHHHHCCC
>Mature Secondary Structure 
TQTAPSSLRSLPPEGAATTNLLDTLQRGLAELDAQGLRRVRRTADTACDAHMRVDGRDI
CCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEE
VGFASNDYLGLAAHPALVAAFAEGARRYGSGSGGSHLLGGHSRAHATLEDELAGFAGGFS
EEECCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCHHHHHHHHHCCCCC
DAPRALYFSTGYMANLAAMTALTGKHATIFSDALNHASLIDGMRLSRANVQVYPHADMAA
CCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEECCCCEEEECCCHHHH
LAALLDASDAETKLIVSDTVFSMDGDIAPLAELVALAERHGAWLVVDDAHGFGVLGPQGR
HHHHHCCCCCCCEEEEECEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
GALAAAALRSPNLIYVGTLGKAAGVAGAFVIAHETVIEWMIQRARSYIFTTAAPPAVAHA
CHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHH
VSASLKVIAGDEGDARRAHLAALIERTRALLRMTRWQPVDSHTAVQPLVIGSNDATLAAM
HHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHH
RSLDAHGLWVPAIRPPTVPVGTSRLRVSLSAAHSFDDLARLEAALIEASEGQTRREAEQP
HHCCCCCEEECCCCCCCCCCCCHHEEEEEHHHCCHHHHHHHHHHHHHCCCCCHHHHHHCC
PRSLRSLPPEGAAVSVGAARRDAEQPPRSLRSLPPEGAAASLGAARRETAA
CHHHHCCCCCCCEEECCHHHCCCCCCCHHHHCCCCCCCHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA