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The map label for this gene is ptsN [H]

Identifier: 115352904

GI number: 115352904

Start: 3147488

End: 3148027

Strand: Direct

Name: ptsN [H]

Synonym: Bamb_2853

Alternate gene names: 115352904

Gene position: 3147488-3148027 (Clockwise)

Preceding gene: 115352903

Following gene: 115352905

Centisome position: 88.5

GC content: 62.41

Gene sequence:

>540_bases
ATGCCAAACGCCTTGATATCGCCGAACATGGAAAATCAGTCTGCCGCCGCAAGGAGACCCCAGGCCGCCCTATCGCCTGC
CAACATGAATCGCCTAGCCAAAATCCTGCCCATAGAGAACGTCGTCATCGACCTCTCCGTCACCAGCAAGAAACGCGTCT
TCGAACAAGCCGGGCTGATGTTCGAGAATCAGAACGGCGTCGCCCGCAGCACCGTCACGGACAACCTGTTCGCGCGCGAG
CGCCTCGGCTCGACCGGGCTCGGCGAAGGCGTCGCGATTCCGCACGGGCGCATCAAGGGGCTCAAGCACCCGCTCGCCGC
ATTCGTGCGGCTCGCCGATGCGATCCCGTTCGAAGCGCCCGACGGCCAGCCGGTCGGTCTCCTGATTTTCCTGCTCGTCC
CCGAGCAAGCTACCCAACAGCACCTCGAGATCCTGTCGGAAATCGCGCAACTGCTATCCGATCGCGACGCGCGCGAGCGT
CTGCACAACGAAACAGATCTCGCCGAGTTGCATCGCGTGCTCACTCAGTGGCAACCTTGA

Upstream 100 bases:

>100_bases
ACCGGTCCGTCCATGCCGCGCCACGCACGCGGCTGTGCTCTATAATGTCCCGGTTATCGCCGGGGGGCGTTGAATGCGGT
AGCTGCGTTGCAGGTTGCCG

Downstream 100 bases:

>100_bases
GTTGATCCGGGCGGAATATTTTCCCGAACGAGGCGGCACGCACCGCCGTCCAGGGCCGCCCGGCGCCGGTCCGGCATGGC
GACGCCCGTCGCCGCGCACA

Product: putative PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugarphosphate [C]

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MPNALISPNMENQSAAARRPQAALSPANMNRLAKILPIENVVIDLSVTSKKRVFEQAGLMFENQNGVARSTVTDNLFARE
RLGSTGLGEGVAIPHGRIKGLKHPLAAFVRLADAIPFEAPDGQPVGLLIFLLVPEQATQQHLEILSEIAQLLSDRDARER
LHNETDLAELHRVLTQWQP

Sequences:

>Translated_179_residues
MPNALISPNMENQSAAARRPQAALSPANMNRLAKILPIENVVIDLSVTSKKRVFEQAGLMFENQNGVARSTVTDNLFARE
RLGSTGLGEGVAIPHGRIKGLKHPLAAFVRLADAIPFEAPDGQPVGLLIFLLVPEQATQQHLEILSEIAQLLSDRDARER
LHNETDLAELHRVLTQWQP
>Mature_178_residues
PNALISPNMENQSAAARRPQAALSPANMNRLAKILPIENVVIDLSVTSKKRVFEQAGLMFENQNGVARSTVTDNLFARER
LGSTGLGEGVAIPHGRIKGLKHPLAAFVRLADAIPFEAPDGQPVGLLIFLLVPEQATQQHLEILSEIAQLLSDRDARERL
HNETDLAELHRVLTQWQP

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=149, Percent_Identity=34.8993288590604, Blast_Score=87, Evalue=5e-19,
Organism=Escherichia coli, GI2367327, Length=121, Percent_Identity=33.8842975206612, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1786951, Length=103, Percent_Identity=33.0097087378641, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 19662; Mature: 19531

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNALISPNMENQSAAARRPQAALSPANMNRLAKILPIENVVIDLSVTSKKRVFEQAGLM
CCCCCCCCCCCCCHHHHHCCHHHCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCE
FENQNGVARSTVTDNLFARERLGSTGLGEGVAIPHGRIKGLKHPLAAFVRLADAIPFEAP
EECCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCC
DGQPVGLLIFLLVPEQATQQHLEILSEIAQLLSDRDARERLHNETDLAELHRVLTQWQP
CCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
PNALISPNMENQSAAARRPQAALSPANMNRLAKILPIENVVIDLSVTSKKRVFEQAGLM
CCCCCCCCCCCCHHHHHCCHHHCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCE
FENQNGVARSTVTDNLFARERLGSTGLGEGVAIPHGRIKGLKHPLAAFVRLADAIPFEAP
EECCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCC
DGQPVGLLIFLLVPEQATQQHLEILSEIAQLLSDRDARERLHNETDLAELHRVLTQWQP
CCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein Npi-phosphohistidine; sugar [C]

Specific reaction: Protein Npi-phosphohistidine + sugar = protein histidine + sugarphosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]