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The map label for this gene is rfaF [H]

Identifier: 115352731

GI number: 115352731

Start: 2951520

End: 2952509

Strand: Direct

Name: rfaF [H]

Synonym: Bamb_2680

Alternate gene names: 115352731

Gene position: 2951520-2952509 (Clockwise)

Preceding gene: 115352730

Following gene: 115352732

Centisome position: 82.99

GC content: 67.78

Gene sequence:

>990_bases
ATGGCGCAGCCGCTTTTTGCGCTGCTGAAAAAAATCCATCCCCGCATCGTGATCGATGCCGTCGCACCCACCTGGGTCGC
GCCGGTGCTCGAGCGGATGCCCGAGATCCACGATGTCTACGCGACCGACCTCGCGCACGGCAAGCTGCAGCTGCTGCGCC
GCTGGCAGCTCGCGAGCGACCTGCGCGACGTCGGCTACGACGCGGCGTACGTGCTGCCGAATTCGCTGAAATCCGCGGTG
ATTCCGTGGCTCGCGAACATTCCGCTGCGGATTGGCTACACGGGCGAGCACCGCTACGGGCTGCTCAACGTGCGGCACGC
GAACCCCGACAAATCGGCCGAGCGGCCGCCGATGACGACCCACTACGCGGCGCTCGCGTATGCGCCGGGCGCGAAGCTGC
CCGAGTCGATGAAGATGCTGCCGCCGCCGCGCCTCGACGCCGACCTGAACGAGACGGCGCGCGTCTCCGCGCGTTTCAAT
CTCGATACACGCAAGCCGCTCGTCGTGTTCTGCCCCGGCGCGGAATACGGCCCGGCCAAGCGCTGGCCACCCGAGCACTT
CGCGACGCTCGCGACGATCGTCCATCAGTCTTTCCCGTATACGCAGATCGTCGCGCTCGGCTCGCAGAAGGACGCCGCCG
CCGCGCAGGCGATCGCCGACCACGCGCCGAACGTGCGCAACCTGTGCGGGCAGACGTCGTTGTCCGAGGCGTGCGCGCTG
ATCGCGCGCGCGAACGCGGTCGTGACCAACGATTCCGGGCTGATGCACGTCGCCGCCGCGCTGCGGCGGCCGCTCGTCGC
GTTGTACGGATCGACTGATCCGCGCCACACCCCTCCGCTGTCGGACCTGGCGAAGGTACAATGGCTTCATCTCGAATGCA
GTCCCTGCTTCGAACGCGAGTGCCCGCTCGGCCATCTGAAATGCCTGCGCGAACTCGGTCCCGAGCAGGTATTCGGCGAT
TTGCGCGGCATGCTCGTCGGGCAGCGCTGA

Upstream 100 bases:

>100_bases
CGAACGCGGTGCATGCGCGCCGTGCTTCATTACGACATCGGAAGTCGACCTGATGCGTCGAGCGCTGGTTATCGCACCGA
ATTGGATCGGTGACGCATTG

Downstream 100 bases:

>100_bases
CCCTGACCGGCAGCGTCGCACGACGCGCCGGGCAATCCGATTCACCGGTGCAAGGCGGCCCCACCCGGGCCGCCGTGCTG
AATACGGCGCGCGACGCGCG

Product: lipopolysaccharide heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MAQPLFALLKKIHPRIVIDAVAPTWVAPVLERMPEIHDVYATDLAHGKLQLLRRWQLASDLRDVGYDAAYVLPNSLKSAV
IPWLANIPLRIGYTGEHRYGLLNVRHANPDKSAERPPMTTHYAALAYAPGAKLPESMKMLPPPRLDADLNETARVSARFN
LDTRKPLVVFCPGAEYGPAKRWPPEHFATLATIVHQSFPYTQIVALGSQKDAAAAQAIADHAPNVRNLCGQTSLSEACAL
IARANAVVTNDSGLMHVAAALRRPLVALYGSTDPRHTPPLSDLAKVQWLHLECSPCFERECPLGHLKCLRELGPEQVFGD
LRGMLVGQR

Sequences:

>Translated_329_residues
MAQPLFALLKKIHPRIVIDAVAPTWVAPVLERMPEIHDVYATDLAHGKLQLLRRWQLASDLRDVGYDAAYVLPNSLKSAV
IPWLANIPLRIGYTGEHRYGLLNVRHANPDKSAERPPMTTHYAALAYAPGAKLPESMKMLPPPRLDADLNETARVSARFN
LDTRKPLVVFCPGAEYGPAKRWPPEHFATLATIVHQSFPYTQIVALGSQKDAAAAQAIADHAPNVRNLCGQTSLSEACAL
IARANAVVTNDSGLMHVAAALRRPLVALYGSTDPRHTPPLSDLAKVQWLHLECSPCFERECPLGHLKCLRELGPEQVFGD
LRGMLVGQR
>Mature_328_residues
AQPLFALLKKIHPRIVIDAVAPTWVAPVLERMPEIHDVYATDLAHGKLQLLRRWQLASDLRDVGYDAAYVLPNSLKSAVI
PWLANIPLRIGYTGEHRYGLLNVRHANPDKSAERPPMTTHYAALAYAPGAKLPESMKMLPPPRLDADLNETARVSARFNL
DTRKPLVVFCPGAEYGPAKRWPPEHFATLATIVHQSFPYTQIVALGSQKDAAAAQAIADHAPNVRNLCGQTSLSEACALI
ARANAVVTNDSGLMHVAAALRRPLVALYGSTDPRHTPPLSDLAKVQWLHLECSPCFERECPLGHLKCLRELGPEQVFGDL
RGMLVGQR

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=339, Percent_Identity=42.4778761061947, Blast_Score=241, Evalue=5e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 36188; Mature: 36057

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPLFALLKKIHPRIVIDAVAPTWVAPVLERMPEIHDVYATDLAHGKLQLLRRWQLASD
CCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LRDVGYDAAYVLPNSLKSAVIPWLANIPLRIGYTGEHRYGLLNVRHANPDKSAERPPMTT
HHHCCCCEEEECCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCH
HYAALAYAPGAKLPESMKMLPPPRLDADLNETARVSARFNLDTRKPLVVFCPGAEYGPAK
HHHHEEECCCCCCCHHHHCCCCCCCCCCCCCHHEEEEEECCCCCCCEEEEECCCCCCCCC
RWPPEHFATLATIVHQSFPYTQIVALGSQKDAAAAQAIADHAPNVRNLCGQTSLSEACAL
CCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHCCCCHHHHHHH
IARANAVVTNDSGLMHVAAALRRPLVALYGSTDPRHTPPLSDLAKVQWLHLECSPCFERE
HHHCCEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHEEEEEEECCCCCCCC
CPLGHLKCLRELGPEQVFGDLRGMLVGQR
CCHHHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AQPLFALLKKIHPRIVIDAVAPTWVAPVLERMPEIHDVYATDLAHGKLQLLRRWQLASD
CCHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LRDVGYDAAYVLPNSLKSAVIPWLANIPLRIGYTGEHRYGLLNVRHANPDKSAERPPMTT
HHHCCCCEEEECCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCH
HYAALAYAPGAKLPESMKMLPPPRLDADLNETARVSARFNLDTRKPLVVFCPGAEYGPAK
HHHHEEECCCCCCCHHHHCCCCCCCCCCCCCHHEEEEEECCCCCCCEEEEECCCCCCCCC
RWPPEHFATLATIVHQSFPYTQIVALGSQKDAAAAQAIADHAPNVRNLCGQTSLSEACAL
CCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHCCCCHHHHHHH
IARANAVVTNDSGLMHVAAALRRPLVALYGSTDPRHTPPLSDLAKVQWLHLECSPCFERE
HHHCCEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHEEEEEEECCCCCCCC
CPLGHLKCLRELGPEQVFGDLRGMLVGQR
CCHHHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]