Click here to switch to the map view.

The map label for this gene is btuF [H]

Identifier: 115352549

GI number: 115352549

Start: 2756903

End: 2757835

Strand: Direct

Name: btuF [H]

Synonym: Bamb_2498

Alternate gene names: 115352549

Gene position: 2756903-2757835 (Clockwise)

Preceding gene: 115352548

Following gene: 115352556

Centisome position: 77.52

GC content: 73.31

Gene sequence:

>933_bases
ATGAACGCTCGCTTGTCGCGCCTGTTGCGACCGCTGCCGCCGCTCGCGATGCTGGTCGCGCTCGCGCTCGCGCACGCGCC
CGTCGCTCACGCCGACGTCACGGCCCGCGACGATGCCGGCAATACCGTCACGCTGCCCGCCCCGGCCCAGCGCGTGATCA
GTCTCGCGCCGCATGCGACCGAGCTGGTCTACGCGGCCGGCGGCGGCGCGAAGCTGGTCGGCACGGTCACGTACAGCGAC
TACCCGCCCGCCGCGCAGGCCGTGCCGCGCGTCGGCGACAACAAGGCCCTCGACCTCGAGCGGATCGCCGCGCTGAAGCC
GGACCTGATCGTCGTCTGGCGGCACGGCAACGCCGAACGGCAGACCGATGCGCTGCGGGCGCTGCACATCCCGCTGTTCT
TCAGCGAACCGAAACATCTCCACGACGTGGCGACGTCCCTGCGCCAGCTCGGTACGCTGCTCGGCACGGCACCCGTCGCC
GACGCGGCCGCGGCATCGTTCTCGCGCGACATCGCGGCGCTGCGCGCACGCTACGCGGCGCGCGCGCCCGTCACGATGTT
CTTCCAGGTATGGGACCGGCCGCTGACGACGCTCAACGGCGCGCACCTGTTCAACGAAGTCATTGCGCTATGCGGCGGCC
GCAACGTGTTCGCGGCGCTGAAGCCGCTCGCGCCGAGCGTCACCGACGAGGCCGTGCTCGCGGCCAATCCGGAAGCGATC
GTGACGACGAGCGCCGGCGCGACGCGCTCGGACGCGCCGCTGCCGAGCCTCGCGCGCTGGCGTGCATGGCCCGCGCTGAC
GGCCGTGGCGCGCAACAACCTGTTCGCGATCGACGGCGATCTGCTGACCCGGCCGTCACCGCGGATCGCGCAAGGCGCGG
CCGCGCTGTGCGAGGATCTCGACGCGGCGCGGGCGCGGCGGCCGGCGCGCTGA

Upstream 100 bases:

>100_bases
CGTCGAGCGTGCGCGTCGGCCTGCCGGGCTGCGAAGCCGAATGGCGGCGGCTCGCCGGTGCGCTCGCGGAGTGCGTCCCG
ATGCTGCAACAGGTATCCGC

Downstream 100 bases:

>100_bases
TTTCGGGCTTCGAAGCGCTTCCGCGTATGCGCTTCAGGCGAGCATGCGCGTCGCCTACGCGTCCCAGTGGACGAGTTCCC
ACGTGCGCGCGGCATCATCC

Product: periplasmic binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MNARLSRLLRPLPPLAMLVALALAHAPVAHADVTARDDAGNTVTLPAPAQRVISLAPHATELVYAAGGGAKLVGTVTYSD
YPPAAQAVPRVGDNKALDLERIAALKPDLIVVWRHGNAERQTDALRALHIPLFFSEPKHLHDVATSLRQLGTLLGTAPVA
DAAAASFSRDIAALRARYAARAPVTMFFQVWDRPLTTLNGAHLFNEVIALCGGRNVFAALKPLAPSVTDEAVLAANPEAI
VTTSAGATRSDAPLPSLARWRAWPALTAVARNNLFAIDGDLLTRPSPRIAQGAAALCEDLDAARARRPAR

Sequences:

>Translated_310_residues
MNARLSRLLRPLPPLAMLVALALAHAPVAHADVTARDDAGNTVTLPAPAQRVISLAPHATELVYAAGGGAKLVGTVTYSD
YPPAAQAVPRVGDNKALDLERIAALKPDLIVVWRHGNAERQTDALRALHIPLFFSEPKHLHDVATSLRQLGTLLGTAPVA
DAAAASFSRDIAALRARYAARAPVTMFFQVWDRPLTTLNGAHLFNEVIALCGGRNVFAALKPLAPSVTDEAVLAANPEAI
VTTSAGATRSDAPLPSLARWRAWPALTAVARNNLFAIDGDLLTRPSPRIAQGAAALCEDLDAARARRPAR
>Mature_310_residues
MNARLSRLLRPLPPLAMLVALALAHAPVAHADVTARDDAGNTVTLPAPAQRVISLAPHATELVYAAGGGAKLVGTVTYSD
YPPAAQAVPRVGDNKALDLERIAALKPDLIVVWRHGNAERQTDALRALHIPLFFSEPKHLHDVATSLRQLGTLLGTAPVA
DAAAASFSRDIAALRARYAARAPVTMFFQVWDRPLTTLNGAHLFNEVIALCGGRNVFAALKPLAPSVTDEAVLAANPEAI
VTTSAGATRSDAPLPSLARWRAWPALTAVARNNLFAIDGDLLTRPSPRIAQGAAALCEDLDAARARRPAR

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=250, Percent_Identity=36.8, Blast_Score=150, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 32758; Mature: 32758

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNARLSRLLRPLPPLAMLVALALAHAPVAHADVTARDDAGNTVTLPAPAQRVISLAPHAT
CCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCEEECCCCHHHHHHHCCCHH
ELVYAAGGGAKLVGTVTYSDYPPAAQAVPRVGDNKALDLERIAALKPDLIVVWRHGNAER
EEEEECCCCCEEEEEEEECCCCCHHHHCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCHH
QTDALRALHIPLFFSEPKHLHDVATSLRQLGTLLGTAPVADAAAASFSRDIAALRARYAA
HHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RAPVTMFFQVWDRPLTTLNGAHLFNEVIALCGGRNVFAALKPLAPSVTDEAVLAANPEAI
CCCHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCCEE
VTTSAGATRSDAPLPSLARWRAWPALTAVARNNLFAIDGDLLTRPSPRIAQGAAALCEDL
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHH
DAARARRPAR
HHHHHCCCCC
>Mature Secondary Structure
MNARLSRLLRPLPPLAMLVALALAHAPVAHADVTARDDAGNTVTLPAPAQRVISLAPHAT
CCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCEEECCCCHHHHHHHCCCHH
ELVYAAGGGAKLVGTVTYSDYPPAAQAVPRVGDNKALDLERIAALKPDLIVVWRHGNAER
EEEEECCCCCEEEEEEEECCCCCHHHHCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCHH
QTDALRALHIPLFFSEPKHLHDVATSLRQLGTLLGTAPVADAAAASFSRDIAALRARYAA
HHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RAPVTMFFQVWDRPLTTLNGAHLFNEVIALCGGRNVFAALKPLAPSVTDEAVLAANPEAI
CCCHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCCEE
VTTSAGATRSDAPLPSLARWRAWPALTAVARNNLFAIDGDLLTRPSPRIAQGAAALCEDL
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHH
DAARARRPAR
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA