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The map label for this gene is metG

Identifier: 115352535

GI number: 115352535

Start: 2739976

End: 2742138

Strand: Direct

Name: metG

Synonym: Bamb_2484

Alternate gene names: 115352535

Gene position: 2739976-2742138 (Clockwise)

Preceding gene: 115352534

Following gene: 115352538

Centisome position: 77.04

GC content: 65.79

Gene sequence:

>2163_bases
ATGTCCGCATCCGACCTGACTTCCGTGCAGGCTACGGCGCCGCAAGGCCGCCGCCAGATCCTCGTTACGTCCGCACTGCC
CTATGCCAACGGCCAGATCCACATCGGCCACCTGGTCGAGTACATCCAGACCGACATCTGGGTGCGGACGCTGCGAATGC
ACGGCCACGAGGTCTACTACATCGGCGCCGACGACACGCACGGCACGCCGGTCATGCTGCGCGCCGAGAAGGAAGGCCTC
ACGCCGAAGCAGCTGATCGACCGCGTGTGGACCGAGCACAAGCGCGACTTCGACAGCTTCGGCGTGTCGTTCGACAACTT
CTACTCGACCGACTCGGACGAGAACCGCGTGCTGAGCGAGAGCATTTATCTCGCGCTGAAGGAAAACGGCCTGATCGCCG
AGCGCGCGATCGAGCAGGCGTACGACCCAGTCAAGGAAATGTTCCTGCCGGACCGCTTCATCAAGGGCGAGTGCCCGAAG
TGCCACGCGAAGGACCAGTACGGCGACAACTGCGAAGTGTGCGGCTCGACCTACCTGCCGACCGAACTGCTGAACCCGTA
TTCGGTGGTGTCGGGTGCGACGCCGGTGCGCAAGACGTCGACGCACTACTTCTTCCGCCTGTCCGACCCGCGCTGCGAGT
CGTTCCTGCGCGAATGGGTCAGCGGCCTCGCGCAGCCCGAAGCCACCAACAAGATGCGCGAATGGCTCGGCGACGCCGGT
GAAGCCAAGCTCGCCGACTGGGACATCTCGCGCGACGCGCCGTACTTCGGCTTCGAGATCCCGGGCGCGCCCGGCAAGTA
CTTCTACGTGTGGCTCGACGCGCCGGTCGGCTACTACGCGAGCTTCAAGAACCTGTGCGACCGCGAGGGCATCGACTTCG
ACGCGTGGATCCGCGCGGGTTCGACGGCCGAGCAGTATCACTTCATCGGCAAGGACATCCTGTATTTCCACACGCTGTTC
TGGCCGGCGATGCTCGAGTTCTCGGGCCATCGCACGCCGACCAACGTGTTCGCGCACGGCTTCCTGACGGTCGACGGCGC
GAAGATGTCGAAGTCGCGCGGCACCTTCATCACCGCGCAGAGCTACATCGACACCGGCCTGAACCCCGAATGGCTGCGCT
ACTACTTCGCCGCGAAGCTGAACGCGACGATGGAAGACATCGACCTGAACCTCGACGACTTCCAGGCGCGCGTGAACAGC
GACCTTGTCGGCAAGTACGTGAACATCGCGAGCCGCGCGGCCGGCTTCCTGATCAAGCGTTTCGACGGCCGCGTGCAGGA
CAGCGCGATGAACCATCCGCTCGTCGCGAAGCTGCGCGATGCGATTGCATCGATCGCCGCGCACTACGAAGGCCGCGAAT
ACAGCCGCGCGCTGCGCCACACGATGGAACTCGCGGACGAAGTAAACGCGTACGTCGACGGTGCGAAGCCGTGGGAACTC
GCGAAGGATCCGGCCAACGCGGTCGCGCTGCACGAAACCTGCAGCGTGAGCCTCGAGGCGTTCCGCCTGCTGTCGCTCGC
GCTGAAGCCGGTGATGCCGCGCGTGGCCGAAGCGGTCGAGGCGTTCTTCGGCGTCGCGCCGCTCGCGTGGGCCGATGCCG
CGAAGCCGCTGTCGTCGGCGCAGCCGATCAAGGCGTACCAGCACCTGATGACGCGTGTCGATCCGAAGCAGATCGACGCG
CTGCTCGCCGCGAACCGCGATTCGCTGCAGGCCGACGCGGCGGGCGCCGCTGCCGCCGGCGCGACCGCCGCCAACGCCGC
GAAGGATGCGAAGAACGCCAAGGCGAACGCGAAGGCGGTTGCCGCGAACGGTGCCGACGACGCTCCGATCTCGATCGACG
ATTTCGCGAAGGTCGACCTGCGCATCGCGAAGATCGTCGCGTGCCAGGCCGTCGAAGGCTCGGACAAGCTGCTGCAGCTC
ACGCTCGACATCGGCGAGGAAAAGACCCGCAACGTGTTCTCGGGCATCAAGTCGGCTTACCAGCCCGAGCAGCTCGTCGG
CAAGCTGACGGTGATGGTCGCGAACCTCGCGCCGCGCAAGATGAAGTTCGGGCTGTCCGAAGGGATGGTGCTCGCCGCGT
CGGCGGCCGACGAGAAGGCCGAACCGGGCCTCTACATCCTCGAGCCGCACAGCGGCGCGAAGCCCGGCATGCGCGTGAAG
TAA

Upstream 100 bases:

>100_bases
CGTCGCGGCACCCCCTGTCGAACACCCGCCCCGGCTGACCGGCGCGCGCCCCTGCTAGAATCGCAGTTTCCGTCGTTTTC
GCCGTTCTTCTACAGACCCT

Downstream 100 bases:

>100_bases
GCAGCACACGCCGCTGGCTGAGCAAGGAAGCCTCGCACGAGATGTGCGGGGCTTTTTTTTATGGCGCGGCCGGACTGCGC
CCCTTGATCACCGCTGGCTG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 720; Mature: 719

Protein sequence:

>720_residues
MSASDLTSVQATAPQGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYIGADDTHGTPVMLRAEKEGL
TPKQLIDRVWTEHKRDFDSFGVSFDNFYSTDSDENRVLSESIYLALKENGLIAERAIEQAYDPVKEMFLPDRFIKGECPK
CHAKDQYGDNCEVCGSTYLPTELLNPYSVVSGATPVRKTSTHYFFRLSDPRCESFLREWVSGLAQPEATNKMREWLGDAG
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCDREGIDFDAWIRAGSTAEQYHFIGKDILYFHTLF
WPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNS
DLVGKYVNIASRAAGFLIKRFDGRVQDSAMNHPLVAKLRDAIASIAAHYEGREYSRALRHTMELADEVNAYVDGAKPWEL
AKDPANAVALHETCSVSLEAFRLLSLALKPVMPRVAEAVEAFFGVAPLAWADAAKPLSSAQPIKAYQHLMTRVDPKQIDA
LLAANRDSLQADAAGAAAAGATAANAAKDAKNAKANAKAVAANGADDAPISIDDFAKVDLRIAKIVACQAVEGSDKLLQL
TLDIGEEKTRNVFSGIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASAADEKAEPGLYILEPHSGAKPGMRVK

Sequences:

>Translated_720_residues
MSASDLTSVQATAPQGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYIGADDTHGTPVMLRAEKEGL
TPKQLIDRVWTEHKRDFDSFGVSFDNFYSTDSDENRVLSESIYLALKENGLIAERAIEQAYDPVKEMFLPDRFIKGECPK
CHAKDQYGDNCEVCGSTYLPTELLNPYSVVSGATPVRKTSTHYFFRLSDPRCESFLREWVSGLAQPEATNKMREWLGDAG
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCDREGIDFDAWIRAGSTAEQYHFIGKDILYFHTLF
WPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNS
DLVGKYVNIASRAAGFLIKRFDGRVQDSAMNHPLVAKLRDAIASIAAHYEGREYSRALRHTMELADEVNAYVDGAKPWEL
AKDPANAVALHETCSVSLEAFRLLSLALKPVMPRVAEAVEAFFGVAPLAWADAAKPLSSAQPIKAYQHLMTRVDPKQIDA
LLAANRDSLQADAAGAAAAGATAANAAKDAKNAKANAKAVAANGADDAPISIDDFAKVDLRIAKIVACQAVEGSDKLLQL
TLDIGEEKTRNVFSGIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASAADEKAEPGLYILEPHSGAKPGMRVK
>Mature_719_residues
SASDLTSVQATAPQGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYIGADDTHGTPVMLRAEKEGLT
PKQLIDRVWTEHKRDFDSFGVSFDNFYSTDSDENRVLSESIYLALKENGLIAERAIEQAYDPVKEMFLPDRFIKGECPKC
HAKDQYGDNCEVCGSTYLPTELLNPYSVVSGATPVRKTSTHYFFRLSDPRCESFLREWVSGLAQPEATNKMREWLGDAGE
AKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCDREGIDFDAWIRAGSTAEQYHFIGKDILYFHTLFW
PAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNSD
LVGKYVNIASRAAGFLIKRFDGRVQDSAMNHPLVAKLRDAIASIAAHYEGREYSRALRHTMELADEVNAYVDGAKPWELA
KDPANAVALHETCSVSLEAFRLLSLALKPVMPRVAEAVEAFFGVAPLAWADAAKPLSSAQPIKAYQHLMTRVDPKQIDAL
LAANRDSLQADAAGAAAAGATAANAAKDAKNAKANAKAVAANGADDAPISIDDFAKVDLRIAKIVACQAVEGSDKLLQLT
LDIGEEKTRNVFSGIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASAADEKAEPGLYILEPHSGAKPGMRVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=565, Percent_Identity=27.787610619469, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI24308436, Length=538, Percent_Identity=27.1375464684015, Blast_Score=163, Evalue=6e-40,
Organism=Homo sapiens, GI215490011, Length=118, Percent_Identity=33.8983050847458, Blast_Score=68, Evalue=4e-11,
Organism=Homo sapiens, GI215490009, Length=118, Percent_Identity=33.8983050847458, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI45006986, Length=118, Percent_Identity=33.8983050847458, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI4507947, Length=144, Percent_Identity=31.9444444444444, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1788432, Length=710, Percent_Identity=52.8169014084507, Blast_Score=746, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=616, Percent_Identity=26.7857142857143, Blast_Score=237, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI212645096, Length=541, Percent_Identity=24.0295748613678, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI71992781, Length=362, Percent_Identity=24.585635359116, Blast_Score=100, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6321704, Length=564, Percent_Identity=28.5460992907801, Blast_Score=235, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6321610, Length=545, Percent_Identity=25.5045871559633, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24655311, Length=524, Percent_Identity=29.9618320610687, Blast_Score=222, Evalue=6e-58,
Organism=Drosophila melanogaster, GI21358483, Length=519, Percent_Identity=27.5529865125241, Blast_Score=138, Evalue=2e-32,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 79328; Mature: 79197

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASDLTSVQATAPQGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYY
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEE
IGADDTHGTPVMLRAEKEGLTPKQLIDRVWTEHKRDFDSFGVSFDNFYSTDSDENRVLSE
EECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHCCCHHHCCCCCCCCHHHHHH
SIYLALKENGLIAERAIEQAYDPVKEMFLPDRFIKGECPKCHAKDQYGDNCEVCGSTYLP
HEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
TELLNPYSVVSGATPVRKTSTHYFFRLSDPRCESFLREWVSGLAQPEATNKMREWLGDAG
HHHCCCHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCDREGIDFDAWIRAG
CCEEECCCCCCCCCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHCCC
STAEQYHFIGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCCCCCCCCCEEEEH
SYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNSDLVGKYVNIASRAAGFLIKR
HHHCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FDGRVQDSAMNHPLVAKLRDAIASIAAHYEGREYSRALRHTMELADEVNAYVDGAKPWEL
HCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AKDPANAVALHETCSVSLEAFRLLSLALKPVMPRVAEAVEAFFGVAPLAWADAAKPLSSA
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCC
QPIKAYQHLMTRVDPKQIDALLAANRDSLQADAAGAAAAGATAANAAKDAKNAKANAKAV
CHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHCCCHHHHHHHHHHCCCCCCEEE
AANGADDAPISIDDFAKVDLRIAKIVACQAVEGSDKLLQLTLDIGEEKTRNVFSGIKSAY
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHCC
QPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASAADEKAEPGLYILEPHSGAKPGMRVK
CHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SASDLTSVQATAPQGRRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYY
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEE
IGADDTHGTPVMLRAEKEGLTPKQLIDRVWTEHKRDFDSFGVSFDNFYSTDSDENRVLSE
EECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHCCCHHHCCCCCCCCHHHHHH
SIYLALKENGLIAERAIEQAYDPVKEMFLPDRFIKGECPKCHAKDQYGDNCEVCGSTYLP
HEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
TELLNPYSVVSGATPVRKTSTHYFFRLSDPRCESFLREWVSGLAQPEATNKMREWLGDAG
HHHCCCHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCDREGIDFDAWIRAG
CCEEECCCCCCCCCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHCCC
STAEQYHFIGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCCCCCCCCCEEEEH
SYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNSDLVGKYVNIASRAAGFLIKR
HHHCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FDGRVQDSAMNHPLVAKLRDAIASIAAHYEGREYSRALRHTMELADEVNAYVDGAKPWEL
HCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AKDPANAVALHETCSVSLEAFRLLSLALKPVMPRVAEAVEAFFGVAPLAWADAAKPLSSA
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCC
QPIKAYQHLMTRVDPKQIDALLAANRDSLQADAAGAAAAGATAANAAKDAKNAKANAKAV
CHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHCCCHHHHHHHHHHCCCCCCEEE
AANGADDAPISIDDFAKVDLRIAKIVACQAVEGSDKLLQLTLDIGEEKTRNVFSGIKSAY
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHCC
QPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASAADEKAEPGLYILEPHSGAKPGMRVK
CHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA