BacMap

Definition	Burkholderia ambifaria AMMD chromosome chromosome 1, complete sequence.
Accession	NC_008390
Length	3,556,545

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The map label for this gene is fabG

Identifier: 115352515

GI number: 115352515

Start: 2713665

End: 2714423

Strand: Direct

Name: fabG

Synonym: Bamb_2464

Alternate gene names: 115352515

Gene position: 2713665-2714423 (Clockwise)

Preceding gene: 115352514

Following gene: 115352516

Centisome position: 76.3

GC content: 68.38

Gene sequence:

>759_bases
ATGCGGTTGAGCGGCAAGACGGCCATCGTCACCGGCGGCGGCTCGGGCTTCGGCGAGGGCATCGCGAAGACCTATGCGCG
CGAAGGCGCGAATGTCGTCGTCAACGACCTGAACGGCGCGGCGGCCGAGCGCGTCGCGAGCGAGATCGCGCTGGCGGGCG
GCAAGGCGATCGCGGTCGCGGGCGACGTGTCCCAAGACGCCGACTGGCGCGCGCTGCTGCAGGCCGCGCTCGACGATTTC
CATTCGGTGCAGATCGTCGTGAACAACGCCGGCACCACGCACCGCAACAAGCCGGTGCTCGACGTGACGGAAGCCGAGTT
CGATCGCGTGTACGCCGTCAACATGAAGAGCCTGTTCTGGAGCGTACAGACCCTGGTGCCGTATTTCCGCGCGCAGGGCG
GCGGCGTGTTCGTGAACGTCGCATCGACGGCCGGCGTGCGGCCGCGCCCGGGCCTCGTCTGGTACAACAGCACGAAGGGC
GCGATGATCACCGCGAGCAAGTCGCTCGCGGCCGAGCTCGGCGCCGACCGCATCCGCGTGAACTGCATCAACCCGGTGCT
CGGCGAGACGGGCCTGATGACCGAGTTCATGGGCTGCGAGGACACGCCGGAGAATCGCCGCCGGTTCCTCGCGACGATTC
CGCTCGGCCGCTTCTCGACGCCGCAGGACATCGCGAACGCGGCCCTGTACCTCGCATCCGACGAAGCCGAGTTCATCACC
GGCGTCTGTCTCGAAGTCGACGGCGGACGCTGCATCTAG

Upstream 100 bases:

>100_bases
GACGATCGCGATCCGGCACGGCTGAACCCGCAGCACGCGGCCGGCAACGACCGGCCGCGCACCGACACACCACGCACGCA
TCCAACAGAGGAGACACACC

Downstream 100 bases:

>100_bases
CCGCACGACCGCGATTACGCCGCGCCGGCACCGGCGCGCGCATCCGCGCAGGGTGCATCCACGCATCGGCATTGCTTCGC
AAGACACCGGATCGGGCGCC

Product: 3-ketoacyl-(acyl-carrier-protein) reductase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: 2,5-DDOL dehydrogenase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MRLSGKTAIVTGGGSGFGEGIAKTYAREGANVVVNDLNGAAAERVASEIALAGGKAIAVAGDVSQDADWRALLQAALDDF
HSVQIVVNNAGTTHRNKPVLDVTEAEFDRVYAVNMKSLFWSVQTLVPYFRAQGGGVFVNVASTAGVRPRPGLVWYNSTKG
AMITASKSLAAELGADRIRVNCINPVLGETGLMTEFMGCEDTPENRRRFLATIPLGRFSTPQDIANAALYLASDEAEFIT
GVCLEVDGGRCI

Sequences:

>Translated_252_residues
MRLSGKTAIVTGGGSGFGEGIAKTYAREGANVVVNDLNGAAAERVASEIALAGGKAIAVAGDVSQDADWRALLQAALDDF
HSVQIVVNNAGTTHRNKPVLDVTEAEFDRVYAVNMKSLFWSVQTLVPYFRAQGGGVFVNVASTAGVRPRPGLVWYNSTKG
AMITASKSLAAELGADRIRVNCINPVLGETGLMTEFMGCEDTPENRRRFLATIPLGRFSTPQDIANAALYLASDEAEFIT
GVCLEVDGGRCI
>Mature_252_residues
MRLSGKTAIVTGGGSGFGEGIAKTYAREGANVVVNDLNGAAAERVASEIALAGGKAIAVAGDVSQDADWRALLQAALDDF
HSVQIVVNNAGTTHRNKPVLDVTEAEFDRVYAVNMKSLFWSVQTLVPYFRAQGGGVFVNVASTAGVRPRPGLVWYNSTKG
AMITASKSLAAELGADRIRVNCINPVLGETGLMTEFMGCEDTPENRRRFLATIPLGRFSTPQDIANAALYLASDEAEFIT
GVCLEVDGGRCI

Specific function: Catalyzes the degradation of 2,5-dichloro-2,5- cyclohexadiene-1,4-diol (2,5-DDOL) into 2,5-dichlorohydroquinone (2,5-DCHQ). LinX appears not to be involved in gamma-HCH degradation pathway [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=250, Percent_Identity=32.4, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI7705925, Length=249, Percent_Identity=33.7349397590361, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI66933014, Length=250, Percent_Identity=31.6, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI5031737, Length=249, Percent_Identity=30.9236947791165, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI15277342, Length=257, Percent_Identity=31.1284046692607, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI126723750, Length=254, Percent_Identity=29.1338582677165, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI19923817, Length=261, Percent_Identity=27.5862068965517, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI40254992, Length=244, Percent_Identity=29.9180327868852, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI33667109, Length=228, Percent_Identity=30.7017543859649, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI59889578, Length=228, Percent_Identity=29.8245614035088, Blast_Score=91, Evalue=7e-19,
Organism=Homo sapiens, GI4504505, Length=246, Percent_Identity=28.4552845528455, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI10190704, Length=250, Percent_Identity=30.4, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI126723191, Length=185, Percent_Identity=32.972972972973, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI210032110, Length=193, Percent_Identity=30.0518134715026, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI142976729, Length=194, Percent_Identity=27.319587628866, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI4758504, Length=259, Percent_Identity=29.7297297297297, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI40807363, Length=201, Percent_Identity=27.363184079602, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI31542939, Length=246, Percent_Identity=26.0162601626016, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI302344755, Length=102, Percent_Identity=35.2941176470588, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI40254986, Length=163, Percent_Identity=26.3803680981595, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI109715829, Length=250, Percent_Identity=28.4, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI83715985, Length=260, Percent_Identity=28.0769230769231, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=38.9558232931727, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI87082100, Length=256, Percent_Identity=33.984375, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1789378, Length=248, Percent_Identity=31.4516129032258, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1787335, Length=251, Percent_Identity=33.4661354581673, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI2367175, Length=248, Percent_Identity=32.6612903225806, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1789208, Length=248, Percent_Identity=29.4354838709677, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI87082160, Length=258, Percent_Identity=31.3953488372093, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1790717, Length=251, Percent_Identity=27.8884462151394, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786812, Length=258, Percent_Identity=31.3953488372093, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788459, Length=248, Percent_Identity=28.2258064516129, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787820, Length=230, Percent_Identity=25.6521739130435, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787526, Length=253, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI2367365, Length=254, Percent_Identity=25.1968503937008, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1789057, Length=261, Percent_Identity=24.904214559387, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17561402, Length=249, Percent_Identity=30.9236947791165, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17555706, Length=249, Percent_Identity=37.3493975903614, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI115534694, Length=250, Percent_Identity=32.8, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17560676, Length=247, Percent_Identity=30.7692307692308, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25147288, Length=249, Percent_Identity=34.9397590361446, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17531453, Length=264, Percent_Identity=29.5454545454545, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI71994600, Length=250, Percent_Identity=31.6, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17559104, Length=263, Percent_Identity=30.4182509505703, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17562904, Length=272, Percent_Identity=30.1470588235294, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17568967, Length=197, Percent_Identity=36.5482233502538, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17562906, Length=264, Percent_Identity=29.9242424242424, Blast_Score=100, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71994604, Length=250, Percent_Identity=31.6, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17538480, Length=272, Percent_Identity=30.8823529411765, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17562910, Length=264, Percent_Identity=30.3030303030303, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17562908, Length=264, Percent_Identity=29.5454545454545, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17560332, Length=266, Percent_Identity=28.9473684210526, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17564282, Length=194, Percent_Identity=30.4123711340206, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17544670, Length=272, Percent_Identity=27.9411764705882, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI193204405, Length=265, Percent_Identity=29.0566037735849, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17560150, Length=267, Percent_Identity=28.4644194756554, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17562990, Length=267, Percent_Identity=28.0898876404494, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17551412, Length=269, Percent_Identity=28.996282527881, Blast_Score=90, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17508895, Length=171, Percent_Identity=32.7485380116959, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17538486, Length=263, Percent_Identity=27.7566539923954, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17560220, Length=265, Percent_Identity=27.9245283018868, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17565030, Length=270, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI72000259, Length=263, Percent_Identity=26.615969581749, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17563726, Length=265, Percent_Identity=25.6603773584906, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193203117, Length=195, Percent_Identity=28.2051282051282, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193203115, Length=191, Percent_Identity=28.2722513089005, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71982365, Length=200, Percent_Identity=26, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17508651, Length=245, Percent_Identity=22.4489795918367, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17567345, Length=196, Percent_Identity=26.0204081632653, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=195, Percent_Identity=32.3076923076923, Blast_Score=90, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323882, Length=242, Percent_Identity=26.8595041322314, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI23397609, Length=252, Percent_Identity=35.3174603174603, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24644339, Length=256, Percent_Identity=33.59375, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI21357041, Length=252, Percent_Identity=34.1269841269841, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24643142, Length=252, Percent_Identity=33.7301587301587, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24639444, Length=251, Percent_Identity=33.0677290836653, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21355319, Length=248, Percent_Identity=30.6451612903226, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI28571526, Length=251, Percent_Identity=33.0677290836653, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI18859837, Length=216, Percent_Identity=31.0185185185185, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24644337, Length=253, Percent_Identity=31.6205533596838, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24640492, Length=194, Percent_Identity=30.9278350515464, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI281364892, Length=197, Percent_Identity=32.48730964467, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17737361, Length=255, Percent_Identity=32.156862745098, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24649181, Length=188, Percent_Identity=29.2553191489362, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21357673, Length=251, Percent_Identity=26.6932270916335, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355631, Length=211, Percent_Identity=29.3838862559242, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI161076933, Length=189, Percent_Identity=26.984126984127, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24584748, Length=189, Percent_Identity=26.984126984127, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24581726, Length=198, Percent_Identity=29.7979797979798, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24665243, Length=201, Percent_Identity=27.363184079602, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI21358495, Length=191, Percent_Identity=27.7486910994764, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 26629; Mature: 26629

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSGKTAIVTGGGSGFGEGIAKTYAREGANVVVNDLNGAAAERVASEIALAGGKAIAVA
CCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEE
GDVSQDADWRALLQAALDDFHSVQIVVNNAGTTHRNKPVLDVTEAEFDRVYAVNMKSLFW
CCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEECCCCCCEEEEEEHHHHHH
SVQTLVPYFRAQGGGVFVNVASTAGVRPRPGLVWYNSTKGAMITASKSLAAELGADRIRV
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEEEECHHHHHHCCCCEEEE
NCINPVLGETGLMTEFMGCEDTPENRRRFLATIPLGRFSTPQDIANAALYLASDEAEFIT
EECCCCCCCCCCHHHHCCCCCCCHHHHEEEEEECCCCCCCHHHHHHEEEEEECCCCCEEE
GVCLEVDGGRCI
EEEEEECCCCCC
>Mature Secondary Structure
MRLSGKTAIVTGGGSGFGEGIAKTYAREGANVVVNDLNGAAAERVASEIALAGGKAIAVA
CCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEE
GDVSQDADWRALLQAALDDFHSVQIVVNNAGTTHRNKPVLDVTEAEFDRVYAVNMKSLFW
CCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEECCCCCCEEEEEEHHHHHH
SVQTLVPYFRAQGGGVFVNVASTAGVRPRPGLVWYNSTKGAMITASKSLAAELGADRIRV
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEEEECHHHHHHCCCCEEEE
NCINPVLGETGLMTEFMGCEDTPENRRRFLATIPLGRFSTPQDIANAALYLASDEAEFIT
EECCCCCCCCCCHHHHCCCCCCCHHHHEEEEEECCCCCCCHHHHHHEEEEEECCCCCEEE
GVCLEVDGGRCI
EEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7515041; 12450824 [H]