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The map label for this gene is tnpR [H]

Identifier: 115352478

GI number: 115352478

Start: 2675840

End: 2676421

Strand: Direct

Name: tnpR [H]

Synonym: Bamb_2427

Alternate gene names: 115352478

Gene position: 2675840-2676421 (Clockwise)

Preceding gene: 115352472

Following gene: 115352479

Centisome position: 75.24

GC content: 57.9

Gene sequence:

>582_bases
ATGTCCCGGACTTTTGCATACGCCCGAGTCAGTACAGCCGACCAGACGGCGACTAACCAGCTCCGCGAGATCAAAGCGGC
TGGCTTTACGGTCGACAAACGTCGGGTTGTGGCGGAGAGTATTTCTGGTAGCGTAAGCGCGGATCAGCGTCCGGGATTTG
CTGAGCTACTGCTTAAGATGGAAGAGGGTGATGTCCTGATCGTGACTAAGCTGGATCGCCTTGGCCGGAACGCAATGGAC
GTGCGCGCAACCGTTGAGGATTTGGCGAGTCGTGGTATTAGGATCCATTGCCTCGCACTCGGTGGTGTTGATCTTACCAG
TGCAGCGGGTCGGATGACGATGCAGGTGCTGAATGCCGTTGCCGAGTTTGAACGAGATTTGATGATTGAGCGGACGCATG
CCGGAATAACACGTGCTCGGGCGGAGGGCAAAGCGATGGGGCGACCAAGCGCCCTTTCGAAACAACAGCGCGGAGAGGTC
CGACAGCGGCTCGCCGAAGGGGCAACTGTGGCGGGGCTTGCGAAGCAGTATGGTACGAGTCGGCAGACGATCATGCGCGT
ACGCTCCAGTACAAACGACTAA

Upstream 100 bases:

>100_bases
CTTTCGGACGCGGTACGCAGCCATGTCCGAAACTGAGGCATTCACAGTTACGTACATAGGGTATCATTCGGACATCCATT
TTGAACACATTACGAGTGAC

Downstream 100 bases:

>100_bases
GGAATGGAAATCGGTACGGCTGTGGTGGGTTGATTTAATAGTGCCGGCGTACTTTTTCTATAATTTAAAGTGAGGATGCC
ATGTCAGACGACGGGGTAAA

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MSRTFAYARVSTADQTATNQLREIKAAGFTVDKRRVVAESISGSVSADQRPGFAELLLKMEEGDVLIVTKLDRLGRNAMD
VRATVEDLASRGIRIHCLALGGVDLTSAAGRMTMQVLNAVAEFERDLMIERTHAGITRARAEGKAMGRPSALSKQQRGEV
RQRLAEGATVAGLAKQYGTSRQTIMRVRSSTND

Sequences:

>Translated_193_residues
MSRTFAYARVSTADQTATNQLREIKAAGFTVDKRRVVAESISGSVSADQRPGFAELLLKMEEGDVLIVTKLDRLGRNAMD
VRATVEDLASRGIRIHCLALGGVDLTSAAGRMTMQVLNAVAEFERDLMIERTHAGITRARAEGKAMGRPSALSKQQRGEV
RQRLAEGATVAGLAKQYGTSRQTIMRVRSSTND
>Mature_192_residues
SRTFAYARVSTADQTATNQLREIKAAGFTVDKRRVVAESISGSVSADQRPGFAELLLKMEEGDVLIVTKLDRLGRNAMDV
RATVEDLASRGIRIHCLALGGVDLTSAAGRMTMQVLNAVAEFERDLMIERTHAGITRARAEGKAMGRPSALSKQQRGEVR
QRLAEGATVAGLAKQYGTSRQTIMRVRSSTND

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787638, Length=189, Percent_Identity=56.6137566137566, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI1787827, Length=189, Percent_Identity=56.0846560846561, Blast_Score=234, Evalue=3e-63,
Organism=Escherichia coli, GI1787404, Length=182, Percent_Identity=35.7142857142857, Blast_Score=103, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 20966; Mature: 20835

Theoretical pI: Translated: 10.87; Mature: 10.87

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTFAYARVSTADQTATNQLREIKAAGFTVDKRRVVAESISGSVSADQRPGFAELLLKM
CCCCEEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHEEE
EEGDVLIVTKLDRLGRNAMDVRATVEDLASRGIRIHCLALGGVDLTSAAGRMTMQVLNAV
CCCCEEEEECHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
AEFERDLMIERTHAGITRARAEGKAMGRPSALSKQQRGEVRQRLAEGATVAGLAKQYGTS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCH
RQTIMRVRSSTND
HHHHHHHHHCCCC
>Mature Secondary Structure 
SRTFAYARVSTADQTATNQLREIKAAGFTVDKRRVVAESISGSVSADQRPGFAELLLKM
CCCEEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHEEE
EEGDVLIVTKLDRLGRNAMDVRATVEDLASRGIRIHCLALGGVDLTSAAGRMTMQVLNAV
CCCCEEEEECHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
AEFERDLMIERTHAGITRARAEGKAMGRPSALSKQQRGEVRQRLAEGATVAGLAKQYGTS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCH
RQTIMRVRSSTND
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3027041 [H]