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The map label for this gene is mscL

Identifier: 115351989

GI number: 115351989

Start: 2137651

End: 2138082

Strand: Direct

Name: mscL

Synonym: Bamb_1938

Alternate gene names: 115351989

Gene position: 2137651-2138082 (Clockwise)

Preceding gene: 115351988

Following gene: 115351990

Centisome position: 60.1

GC content: 58.56

Gene sequence:

>432_bases
ATGAGCATAATCAAGGAATTCAAGGAGTTCGCCGTCAAAGGCAACGTGATGGATCTCGCCGTCGGCGTGATCATCGGCGG
CGCGTTCTCGAAGATCGTCGACTCGGTCGTGAAAGACCTCATCATGCCGGTGATCGGCGTGCTGACCGGCGGTCTGGATT
TCTCGAACAAGTTCGTTCTGCTCGGCACCATCCCCCCGACGTTCAAGGGTAATCCCGATTCGTTCAAGGACCTGCAGGCC
GCGGGCGTCGCCGCGTTCGGCTACGGCTCGTTCATCACCGTGGCGATCAACTTCGTGATCCTTGCGTTCATCATTTTCCT
GATGGTGAAATTCATCAACAAGCTGCGCAAGCCGGAAGAAGCCGCGCCGGCCGCGACGCCGGAAGACATCGTGCTGCTGC
GCGAGATCCGCGATTCGCTCAAGCAACGCTGA

Upstream 100 bases:

>100_bases
ATCATGCGGCCATCGGCGTGCCTCCGATCCCATCCGACGTTTCGCAGCCCTGCGCGTCGCGGCACGCCTTCCCCTAGCCT
CAATCAGGAGAAAGCCGCTC

Downstream 100 bases:

>100_bases
GCACCGCGCCACGCTGCATCTGCCGGGCCCGCACAATGCGGGCCTTTTTCATGCGCGTGCCGCGCGCATGCGTGCGCCGC
GCGCCGCCGCGCGACGCTTA

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 142

Protein sequence:

>143_residues
MSIIKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVLTGGLDFSNKFVLLGTIPPTFKGNPDSFKDLQA
AGVAAFGYGSFITVAINFVILAFIIFLMVKFINKLRKPEEAAPAATPEDIVLLREIRDSLKQR

Sequences:

>Translated_143_residues
MSIIKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVLTGGLDFSNKFVLLGTIPPTFKGNPDSFKDLQA
AGVAAFGYGSFITVAINFVILAFIIFLMVKFINKLRKPEEAAPAATPEDIVLLREIRDSLKQR
>Mature_142_residues
SIIKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVLTGGLDFSNKFVLLGTIPPTFKGNPDSFKDLQAA
GVAAFGYGSFITVAINFVILAFIIFLMVKFINKLRKPEEAAPAATPEDIVLLREIRDSLKQR

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=146, Percent_Identity=53.4246575342466, Blast_Score=120, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_BURA4 (B1YS18)

Other databases:

- EMBL:   CP001025
- RefSeq:   YP_001808563.1
- ProteinModelPortal:   B1YS18
- SMR:   B1YS18
- GeneID:   6176651
- GenomeReviews:   CP001025_GR
- KEGG:   bac:BamMC406_1865
- HOGENOM:   HBG727604
- OMA:   IKATRCP
- ProtClustDB:   PRK13952
- BioCyc:   BAMB398577:BAMMC406_1865-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15477; Mature: 15345

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x16c3f9a8)-; HASH(0x146e6d20)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVLTGGLDFSNKFVL
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
LGTIPPTFKGNPDSFKDLQAAGVAAFGYGSFITVAINFVILAFIIFLMVKFINKLRKPEE
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AAPAATPEDIVLLREIRDSLKQR
CCCCCCHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
SIIKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVLTGGLDFSNKFVL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
LGTIPPTFKGNPDSFKDLQAAGVAAFGYGSFITVAINFVILAFIIFLMVKFINKLRKPEE
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AAPAATPEDIVLLREIRDSLKQR
CCCCCCHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA