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The map label for this gene is ramA [H]

Identifier: 115351690

GI number: 115351690

Start: 1812328

End: 1813173

Strand: Direct

Name: ramA [H]

Synonym: Bamb_1638

Alternate gene names: 115351690

Gene position: 1812328-1813173 (Clockwise)

Preceding gene: 115351688

Following gene: 115351691

Centisome position: 50.96

GC content: 66.31

Gene sequence:

>846_bases
ATGCAGGTGGAACTCGCGCAACTGACGCTCGTCGACGGCGACGTCGTGCACAACACGCGCAAGGTGATCGATACGATCGA
ACACGTCGACAGCGCAGGCGGTACCCGGCTGATCGTGTTCCCCGAAACGACGCTGTCCGGCTTCCCGACGCGGGACACGG
TCGCCGACGTCGCGCAGCCGCTCGATGGCCCCGCATTGTCCGCGGTACGCGACGCGGCGCGGCAAAAGGGCGTTGCCGTG
GCCGTGGGCCTCGCCGAGCGCGACGGTGGGCAGTTCTACAACACCACGGTGCTCGTCGACGAGCAGGGCGATATCGTGCT
GCGCTATCGCAAGACGCATCTTTGGGCGTCGGATGTCGGCGTCTTCACGCCGGGCGATCGCTTCGAGACCTGCAGCTGGA
ACGGTCTGACGGTCGGCCTGCTGATCTGCTACGACATCGAATTCCCCGAAACGGCACGCGCCGTCGGTGCGCTCGATGCC
GATCTGCTGATCGTCACGAACGGCAACATGGACCCGTTCGGTCCGGTGCATCGCCGTGCGATCGCCGCGCGCGCGATGGA
AAACCAGATGTTCGCGGTCATGGTGAACCGCTGCGGGTCCGGCGACGACAACCTGACGTTCGCCGGTCTTTCCACGCTCG
TCGATCCCTTTGGCGACACGGTGATCGAGCTGGGCCGGGACGAGGCGGTCGTGCCCGCGAGCGTCGATCCGACGCGTCTC
GAAGCGAGTCGCGAGCACTACAACTATCTGCACGATGCCCGTGTGGCGCTCGGTCTGTCGCCCGTCGAACCGGCGGGCGG
GAACCGCGTCGTCGTGATCGAACCGCGCCGTCCTCGCACCAAATAG

Upstream 100 bases:

>100_bases
AATCAGCCTCACTTCGTCACGCAGCAGACCATGCGGCGCTTTGCTCGGCGCGCGTGGCATCAGACGTGCAACTGAAAGGC
GATTGGAAGGAGATGACTTC

Downstream 100 bases:

>100_bases
CCGGAATCCACGGTCCATTCAACGCATGGGGCAGGGCGCAATGCCCTGCACGTGCTCGCCCGTCCCATTGAAATGCCGGC
CCGACGGCATACAGGCTTTC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MQVELAQLTLVDGDVVHNTRKVIDTIEHVDSAGGTRLIVFPETTLSGFPTRDTVADVAQPLDGPALSAVRDAARQKGVAV
AVGLAERDGGQFYNTTVLVDEQGDIVLRYRKTHLWASDVGVFTPGDRFETCSWNGLTVGLLICYDIEFPETARAVGALDA
DLLIVTNGNMDPFGPVHRRAIAARAMENQMFAVMVNRCGSGDDNLTFAGLSTLVDPFGDTVIELGRDEAVVPASVDPTRL
EASREHYNYLHDARVALGLSPVEPAGGNRVVVIEPRRPRTK

Sequences:

>Translated_281_residues
MQVELAQLTLVDGDVVHNTRKVIDTIEHVDSAGGTRLIVFPETTLSGFPTRDTVADVAQPLDGPALSAVRDAARQKGVAV
AVGLAERDGGQFYNTTVLVDEQGDIVLRYRKTHLWASDVGVFTPGDRFETCSWNGLTVGLLICYDIEFPETARAVGALDA
DLLIVTNGNMDPFGPVHRRAIAARAMENQMFAVMVNRCGSGDDNLTFAGLSTLVDPFGDTVIELGRDEAVVPASVDPTRL
EASREHYNYLHDARVALGLSPVEPAGGNRVVVIEPRRPRTK
>Mature_281_residues
MQVELAQLTLVDGDVVHNTRKVIDTIEHVDSAGGTRLIVFPETTLSGFPTRDTVADVAQPLDGPALSAVRDAARQKGVAV
AVGLAERDGGQFYNTTVLVDEQGDIVLRYRKTHLWASDVGVFTPGDRFETCSWNGLTVGLLICYDIEFPETARAVGALDA
DLLIVTNGNMDPFGPVHRRAIAARAMENQMFAVMVNRCGSGDDNLTFAGLSTLVDPFGDTVIELGRDEAVVPASVDPTRL
EASREHYNYLHDARVALGLSPVEPAGGNRVVVIEPRRPRTK

Specific function: Hydrolyzes (R)-piperazine-2-carboxamide and (R)- piperazine-2-tert-butylcarboxamide with strict R- stereoselectivity. Also active towards beta-alaninamide, piperidine-3-carboxmide, D-glutaminamide and slightly active towards L-glutaminamide and piperidine

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=226, Percent_Identity=26.9911504424779, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI297632348, Length=240, Percent_Identity=27.9166666666667, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI297632350, Length=232, Percent_Identity=28.448275862069, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI5031947, Length=240, Percent_Identity=27.9166666666667, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1786412, Length=257, Percent_Identity=26.8482490272374, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323383, Length=263, Percent_Identity=26.9961977186312, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17933642, Length=242, Percent_Identity=26.0330578512397, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21355835, Length=260, Percent_Identity=26.5384615384615, Blast_Score=81, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.100 [H]

Molecular weight: Translated: 30351; Mature: 30351

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVELAQLTLVDGDVVHNTRKVIDTIEHVDSAGGTRLIVFPETTLSGFPTRDTVADVAQP
CCEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHCC
LDGPALSAVRDAARQKGVAVAVGLAERDGGQFYNTTVLVDEQGDIVLRYRKTHLWASDVG
CCCHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEEEECCCCEEEEEEEEEEEECCCC
VFTPGDRFETCSWNGLTVGLLICYDIEFPETARAVGALDADLLIVTNGNMDPFGPVHRRA
EECCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHCCCCCEEEEECCCCCCCCHHHHHH
IAARAMENQMFAVMVNRCGSGDDNLTFAGLSTLVDPFGDTVIELGRDEAVVPASVDPTRL
HHHHHHHCHHEEEEEECCCCCCCCEEEHHHHHHHHHCCCHHHHCCCCCEEECCCCCHHHH
EASREHYNYLHDARVALGLSPVEPAGGNRVVVIEPRRPRTK
HHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MQVELAQLTLVDGDVVHNTRKVIDTIEHVDSAGGTRLIVFPETTLSGFPTRDTVADVAQP
CCEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHCC
LDGPALSAVRDAARQKGVAVAVGLAERDGGQFYNTTVLVDEQGDIVLRYRKTHLWASDVG
CCCHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEEEECCCCEEEEEEEEEEEECCCC
VFTPGDRFETCSWNGLTVGLLICYDIEFPETARAVGALDADLLIVTNGNMDPFGPVHRRA
EECCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHCCCCCEEEEECCCCCCCCHHHHHH
IAARAMENQMFAVMVNRCGSGDDNLTFAGLSTLVDPFGDTVIELGRDEAVVPASVDPTRL
HHHHHHHCHHEEEEEECCCCCCCCEEEHHHHHHHHHCCCHHHHCCCCCEEECCCCCHHHH
EASREHYNYLHDARVALGLSPVEPAGGNRVVVIEPRRPRTK
HHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA