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The map label for this gene is emrB [H]

Identifier: 115351688

GI number: 115351688

Start: 1809295

End: 1810833

Strand: Direct

Name: emrB [H]

Synonym: Bamb_1635

Alternate gene names: 115351688

Gene position: 1809295-1810833 (Clockwise)

Preceding gene: 115351687

Following gene: 115351690

Centisome position: 50.87

GC content: 66.99

Gene sequence:

>1539_bases
ATGAGCGGGCCGATGCCGGCCGCCGATGGCGGCTTTCGCCCACCGAGCGTCGCGCTGACGACGTTCGGTCTCGCGCTCGC
TGTCTTCATGCAGGTGCTCGACGGCACCGTCGCGAACGTATCGCTGCCGACGATCGCCGGCAATTTCGGCGTGAGCACGA
CGCAGAGCGCATGGGTCGTCACCACGTTCTCGGTCAGCATTGCAATCGCGCTGCCGCTGACCGGCTTTCTCGTCAAGCGC
ATCGGGCAGGTCCGGCTGTTCGTGTGGGCCACGCTCGCCTTCACGTTCGCGTCGCTGCTGTGCGGCCTCGCGCAAAACCT
GCCGCAGCTCGTCGCGTTCCGCGCGCTGCAGGGCCTGGTGGCCGGGCCGATGATCCCCACCACGCAAGCGTTGATGTTGT
CGATCTACCCGCCGCAGCGACGCGGCTTCGCGCTGTCGATGATCGCGATGGTCACCGTCGTCGCGCCGATCGCGGGCCCC
GTGTTCGGCGGCTGGATCACCGAGCATTACTCGTGGCGCTGGGCGTTCCTGATCAATCTGCCGATCGGCGTGTTCGCCGC
GCTCTGCGTCTTCGCGCAGATGCGTGCGCGCGTCGAAACGACCGTCGCCGCGCGCGTCGACTATGTCAGCCTCGCCGCGC
TGGTCGTCGGCGTCGGCGCGCTGCAGATCGTGCTCGACAAGGGCAACGAGGCCGACTGGTTCAACTCCACGTTCATCGTC
GTGATGTCGGTGGTCGCCGCATTCGGCATCGCGCTGTTCCTGATCTGGGAACTCAACGAACCGAATCCGATCGTGAACCT
GCGGCTTTTCGCCCATCGCAACTTCGCGGCAGGCACGCTGACGCTCGTGCTCGCATACTCGGCGTTCTTCGCGGTCAACG
TGATCGTCCCGCAATGGTTGCAGCGCACGCTCGGCTACACGGCGTTCTGGGCCGGGCTTGCCGTCGCGCCGATGGGCGTG
ATTCCGGTGCTGACGACGCCGTTCATGGGCAAGTACGCGCCGCGCTTCAACATGCGTGTGCTCGTGTGCTGCGCATTCGC
GATCCTCGGCGCGTCGTCGTTCCTGCGCGCCGGCTTCGTGCCCGACGTCGACTTCACGCACATCGCGCTGATCCAGTTGC
TGCAGGGCCTCGGCCTCGCGCTGTTCATCATGCCGATCAACAGCATCCTGCTGTCCGACCTGAAACCCGACGAGATCGCC
GCCGGGTCCGGCCTGTCGACCTTCCTGCGCACGCTCGGCGCGAGCTTCGCGGTATCGATCACGTCGTTCCTGTGGGATCG
GCGCGCCGTCCTGCACCACGCGCAACTCAGCGAGCACGTGAGCGCGTTCGATCCGGCCGTGCGCGCCGAGGTCGCGCGGC
TCGGCGGCGGCGACCCGCAACGGGCCGCCGCGCTACTGGGCAATCTGGTCGACGCGCAGGCCTATCAGATTTCATTCAAC
GACGTTTCATTCGCGCTCGGCTGCGTCTATCTCGCCGTAATGGCGGTGGTATGGCTCGCCCGCCCGCCGTTCGGCCCCGG
TGCCGGCGCGAAACGATGA

Upstream 100 bases:

>100_bases
GCACCGACGTGTACCGTGAGGAGCTCGCGCGCGCCGACGGGCTGATCGCCGATGTCCTGCATGCAAACCTGCCCGCGACG
GCGCGCGCGGAGCGTGTGCG

Downstream 100 bases:

>100_bases
AACGGGCGCCGCGCGGCCCGTTTCGACCCATGCTGTTCAACTCGAAGGAGATCGACCTGATGCATACCCAACACGCTGGA
AAGATGTCGTCCGCACTGGC

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MSGPMPAADGGFRPPSVALTTFGLALAVFMQVLDGTVANVSLPTIAGNFGVSTTQSAWVVTTFSVSIAIALPLTGFLVKR
IGQVRLFVWATLAFTFASLLCGLAQNLPQLVAFRALQGLVAGPMIPTTQALMLSIYPPQRRGFALSMIAMVTVVAPIAGP
VFGGWITEHYSWRWAFLINLPIGVFAALCVFAQMRARVETTVAARVDYVSLAALVVGVGALQIVLDKGNEADWFNSTFIV
VMSVVAAFGIALFLIWELNEPNPIVNLRLFAHRNFAAGTLTLVLAYSAFFAVNVIVPQWLQRTLGYTAFWAGLAVAPMGV
IPVLTTPFMGKYAPRFNMRVLVCCAFAILGASSFLRAGFVPDVDFTHIALIQLLQGLGLALFIMPINSILLSDLKPDEIA
AGSGLSTFLRTLGASFAVSITSFLWDRRAVLHHAQLSEHVSAFDPAVRAEVARLGGGDPQRAAALLGNLVDAQAYQISFN
DVSFALGCVYLAVMAVVWLARPPFGPGAGAKR

Sequences:

>Translated_512_residues
MSGPMPAADGGFRPPSVALTTFGLALAVFMQVLDGTVANVSLPTIAGNFGVSTTQSAWVVTTFSVSIAIALPLTGFLVKR
IGQVRLFVWATLAFTFASLLCGLAQNLPQLVAFRALQGLVAGPMIPTTQALMLSIYPPQRRGFALSMIAMVTVVAPIAGP
VFGGWITEHYSWRWAFLINLPIGVFAALCVFAQMRARVETTVAARVDYVSLAALVVGVGALQIVLDKGNEADWFNSTFIV
VMSVVAAFGIALFLIWELNEPNPIVNLRLFAHRNFAAGTLTLVLAYSAFFAVNVIVPQWLQRTLGYTAFWAGLAVAPMGV
IPVLTTPFMGKYAPRFNMRVLVCCAFAILGASSFLRAGFVPDVDFTHIALIQLLQGLGLALFIMPINSILLSDLKPDEIA
AGSGLSTFLRTLGASFAVSITSFLWDRRAVLHHAQLSEHVSAFDPAVRAEVARLGGGDPQRAAALLGNLVDAQAYQISFN
DVSFALGCVYLAVMAVVWLARPPFGPGAGAKR
>Mature_511_residues
SGPMPAADGGFRPPSVALTTFGLALAVFMQVLDGTVANVSLPTIAGNFGVSTTQSAWVVTTFSVSIAIALPLTGFLVKRI
GQVRLFVWATLAFTFASLLCGLAQNLPQLVAFRALQGLVAGPMIPTTQALMLSIYPPQRRGFALSMIAMVTVVAPIAGPV
FGGWITEHYSWRWAFLINLPIGVFAALCVFAQMRARVETTVAARVDYVSLAALVVGVGALQIVLDKGNEADWFNSTFIVV
MSVVAAFGIALFLIWELNEPNPIVNLRLFAHRNFAAGTLTLVLAYSAFFAVNVIVPQWLQRTLGYTAFWAGLAVAPMGVI
PVLTTPFMGKYAPRFNMRVLVCCAFAILGASSFLRAGFVPDVDFTHIALIQLLQGLGLALFIMPINSILLSDLKPDEIAA
GSGLSTFLRTLGASFAVSITSFLWDRRAVLHHAQLSEHVSAFDPAVRAEVARLGGGDPQRAAALLGNLVDAQAYQISFND
VSFALGCVYLAVMAVVWLARPPFGPGAGAKR

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=492, Percent_Identity=45.7317073170732, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1788710, Length=487, Percent_Identity=40.6570841889117, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1790195, Length=401, Percent_Identity=28.927680798005, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1788392, Length=404, Percent_Identity=27.7227722772277, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI87081983, Length=406, Percent_Identity=26.3546798029557, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1788509, Length=136, Percent_Identity=30.1470588235294, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=130, Percent_Identity=32.3076923076923, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=182, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54626; Mature: 54495

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPMPAADGGFRPPSVALTTFGLALAVFMQVLDGTVANVSLPTIAGNFGVSTTQSAWVV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCEECCCCCCCCCCEEEE
TTFSVSIAIALPLTGFLVKRIGQVRLFVWATLAFTFASLLCGLAQNLPQLVAFRALQGLV
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGPMIPTTQALMLSIYPPQRRGFALSMIAMVTVVAPIAGPVFGGWITEHYSWRWAFLINL
CCCCCCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCEEEEEEEEC
PIGVFAALCVFAQMRARVETTVAARVDYVSLAALVVGVGALQIVLDKGNEADWFNSTFIV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHH
VMSVVAAFGIALFLIWELNEPNPIVNLRLFAHRNFAAGTLTLVLAYSAFFAVNVIVPQWL
HHHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
QRTLGYTAFWAGLAVAPMGVIPVLTTPFMGKYAPRFNMRVLVCCAFAILGASSFLRAGFV
HHHHCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
PDVDFTHIALIQLLQGLGLALFIMPINSILLSDLKPDEIAAGSGLSTFLRTLGASFAVSI
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHCCHHHHHH
TSFLWDRRAVLHHAQLSEHVSAFDPAVRAEVARLGGGDPQRAAALLGNLVDAQAYQISFN
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEC
DVSFALGCVYLAVMAVVWLARPPFGPGAGAKR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SGPMPAADGGFRPPSVALTTFGLALAVFMQVLDGTVANVSLPTIAGNFGVSTTQSAWVV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCEECCCCCCCCCCEEEE
TTFSVSIAIALPLTGFLVKRIGQVRLFVWATLAFTFASLLCGLAQNLPQLVAFRALQGLV
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGPMIPTTQALMLSIYPPQRRGFALSMIAMVTVVAPIAGPVFGGWITEHYSWRWAFLINL
CCCCCCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCEEEEEEEEC
PIGVFAALCVFAQMRARVETTVAARVDYVSLAALVVGVGALQIVLDKGNEADWFNSTFIV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHH
VMSVVAAFGIALFLIWELNEPNPIVNLRLFAHRNFAAGTLTLVLAYSAFFAVNVIVPQWL
HHHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
QRTLGYTAFWAGLAVAPMGVIPVLTTPFMGKYAPRFNMRVLVCCAFAILGASSFLRAGFV
HHHHCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
PDVDFTHIALIQLLQGLGLALFIMPINSILLSDLKPDEIAAGSGLSTFLRTLGASFAVSI
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHCCHHHHHH
TSFLWDRRAVLHHAQLSEHVSAFDPAVRAEVARLGGGDPQRAAALLGNLVDAQAYQISFN
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEEC
DVSFALGCVYLAVMAVVWLARPPFGPGAGAKR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]