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The map label for this gene is gacS [H]

Identifier: 115351649

GI number: 115351649

Start: 1768676

End: 1770793

Strand: Direct

Name: gacS [H]

Synonym: Bamb_1596

Alternate gene names: 115351649

Gene position: 1768676-1770793 (Clockwise)

Preceding gene: 115351648

Following gene: 115351650

Centisome position: 49.73

GC content: 67.99

Gene sequence:

>2118_bases
ATGATGCTCGACGAAATCGAGCGGCGCGACGTCGATCTGCAGGCCGCGATCGCGACGGCCGAAGCCGCGAATTCCGCGAA
ATCGGATTTTCTCGCGAGGATGAGCCATGAGATCCGCACGCCGATGAATGGCGTGCTCGGCATGACCGAGCTGCTGCAGC
GCACGCGGCTGACGTCGAAGCAACGCAAGTTCGTCGACACCGTGTATCGATCCGGCCAGACGCTGCTCGTCATCATCGAC
GACATTCTCGATTTTTCGAAGATCGAGGCGGGCAAGCTGACCTTGGAGCGCATCGATTTCAACCTGCATCAATTGCTCGA
CGACGTCGTCGCGCTGATGGAGCCCAATGCCCAACGCAAGGAGCTCGAGCTCGTGTACCAGCGCGCCGATTCCGTTCCGC
AGCGCATGCGCGGCGACCCGGTCCGCCTGCGCCAGATCCTGACGAACCTGCTCGGCAACGCGATCAAGTTCACCGAGCAC
GGGCACGTCGCGCTGAGCGCCGCGTATTCGGACGACGGGCGCATCGTGTTCAAGATCGCCGACAGCGGCATCGGCATCGC
GCCCGATGTCGCCGCGCAGTTGTTCCGGCCGTTCCAGCAGGCCGACAGTTCGACCGCGCGCAAATACGGCGGCACCGGCC
TCGGGCTCGTGATTTCCCGCCAGCTCGCCGAGATGATGGGCGGCACGCTCGCGCTCGACTCGACGCCCGGCAAGGGCTCG
GTATTCACCGTGACGACGCCGTTCGAGCCGGCATCCGGCGAGGTCGGCGTCGCGGCACACGGAAGGCCGTCGCTCGCCGG
CCTGCGGGTGCTGATCGTCGACGACAGCGAGACCGATCGCAGCGTGCTGCTCGAACATGCGATCGAATGGCACCTGTGCG
TCGCCTCGGCGATCGGCGGCGACGATGCGCTGTCGCAGTTGCGGGCCGCCGTCGCCGACGGTGCGCCGTTCGACCTCGCG
ATCATCGACGTGCGGATGCCGGGCATGGACGGCATCTCGCTCGTGCAGCACATCCGCCGCGATCCGGCGCTGCGTGCGCT
GCGGATCATCACGCTGACCGCGTTCGAGATGATGGACAGCATCCAGGTCGCGCACGAACTCGACATCGCCGCCCGGCTGA
CGAAACCGCTCCATGGCAACGATCTCTATACGGCGATCGCGGCGGCGGCGGTGCACGTGATGCCGCCCCGGCTGGCGTCG
GCCGCTCCGGTGTCGGATGAAGCCGGGGCGGACCCGGGCAGGCCGCTCGACGAGATCGCCGCGCGCGTGCTGCTGGTGGA
GGACAACGCGGTGAATCAGCAGATCGCGCTCGCGATGCTCGAAGACACCGACTATCGGGTCGCCGTGGCCGACGACGGCG
AGCAGGCATTGAAGATGCTCGAGCAAGACGCGTTCGACGTGGTGCTGATGGACTGTCAGATGCCGGTGCTGGACGGCTTC
GAGGCGACGCGGCGGCTGCGGCGGCGCGAAGCCGAAGCCGGCGCGCCGCGCCTGCCGGTCATCGCGCTGACGGCCAATGC
GCTCAGCGGTGACCGCGAGCGCTGCCTGGCCGCCGGCATGGACGACTATCTCGGCAAGCCGTTCCGCCGCGACGCGTTGC
TGCAGACGCTCGCACGCCATGTTCGCGGCGGCGCACACGTGTCGGCCGCCGCGAGCGAAAACGCGGAAGCGGTGGCCCGG
ACCGGTATCGAAACGAACCTCGAAGCGAGCATCGAAACGCGTCCCGCAACGAGCGCGGCAGCGGCCGCCGATCCGGACGA
GGACGCGAACGTGATCGACCGCAAGGCGCTCGATGCATTGCGCGCGCTCCAGCGGCCCGGTCGCCCGGACGTGCTGACGC
GGATCATCGATCAATTCACGATGGACGCGCCGCGGTTGATTCGCGACATGCACGCGGCGGTCGAAGCGGGCGATGCCGAC
GCGCTGAAGCTTGCCGCCCATACGCTGAAATCGAGCAGCGCGAACGTCGGTGCGCATCTGCTGTCGGCCCGCTGCCGCGA
GATCGAGCGGCTGGCGCGCGCCGCCGACGTGACCGCGGCCGTCGAGCTCGTCGCCGGCACGGATGCGGAATTCGAACAGG
CGCACGCCGCGCTGATCGCGGAGCGCGTCGAAAGTTGA

Upstream 100 bases:

>100_bases
GACGCGCACGATGGCGCGCATCCGCAGCGGCAGCGACCTGTCGCTGCGCATGTCCCACAAGGGCCGCGACGAAATCGCGG
CGCTGATCGCCGGCTTCAAC

Downstream 100 bases:

>100_bases
CTGGCCGCCGGCCGATCGCCACTGCGATCGCGAAGCGACAGATACAGGAACAGGAGGACGGTCGTGAACCACGAACATGC
CGATTTGCCGGCGATTCTGA

Product: multi-sensor hybrid histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MMLDEIERRDVDLQAAIATAEAANSAKSDFLARMSHEIRTPMNGVLGMTELLQRTRLTSKQRKFVDTVYRSGQTLLVIID
DILDFSKIEAGKLTLERIDFNLHQLLDDVVALMEPNAQRKELELVYQRADSVPQRMRGDPVRLRQILTNLLGNAIKFTEH
GHVALSAAYSDDGRIVFKIADSGIGIAPDVAAQLFRPFQQADSSTARKYGGTGLGLVISRQLAEMMGGTLALDSTPGKGS
VFTVTTPFEPASGEVGVAAHGRPSLAGLRVLIVDDSETDRSVLLEHAIEWHLCVASAIGGDDALSQLRAAVADGAPFDLA
IIDVRMPGMDGISLVQHIRRDPALRALRIITLTAFEMMDSIQVAHELDIAARLTKPLHGNDLYTAIAAAAVHVMPPRLAS
AAPVSDEAGADPGRPLDEIAARVLLVEDNAVNQQIALAMLEDTDYRVAVADDGEQALKMLEQDAFDVVLMDCQMPVLDGF
EATRRLRRREAEAGAPRLPVIALTANALSGDRERCLAAGMDDYLGKPFRRDALLQTLARHVRGGAHVSAAASENAEAVAR
TGIETNLEASIETRPATSAAAAADPDEDANVIDRKALDALRALQRPGRPDVLTRIIDQFTMDAPRLIRDMHAAVEAGDAD
ALKLAAHTLKSSSANVGAHLLSARCREIERLARAADVTAAVELVAGTDAEFEQAHAALIAERVES

Sequences:

>Translated_705_residues
MMLDEIERRDVDLQAAIATAEAANSAKSDFLARMSHEIRTPMNGVLGMTELLQRTRLTSKQRKFVDTVYRSGQTLLVIID
DILDFSKIEAGKLTLERIDFNLHQLLDDVVALMEPNAQRKELELVYQRADSVPQRMRGDPVRLRQILTNLLGNAIKFTEH
GHVALSAAYSDDGRIVFKIADSGIGIAPDVAAQLFRPFQQADSSTARKYGGTGLGLVISRQLAEMMGGTLALDSTPGKGS
VFTVTTPFEPASGEVGVAAHGRPSLAGLRVLIVDDSETDRSVLLEHAIEWHLCVASAIGGDDALSQLRAAVADGAPFDLA
IIDVRMPGMDGISLVQHIRRDPALRALRIITLTAFEMMDSIQVAHELDIAARLTKPLHGNDLYTAIAAAAVHVMPPRLAS
AAPVSDEAGADPGRPLDEIAARVLLVEDNAVNQQIALAMLEDTDYRVAVADDGEQALKMLEQDAFDVVLMDCQMPVLDGF
EATRRLRRREAEAGAPRLPVIALTANALSGDRERCLAAGMDDYLGKPFRRDALLQTLARHVRGGAHVSAAASENAEAVAR
TGIETNLEASIETRPATSAAAAADPDEDANVIDRKALDALRALQRPGRPDVLTRIIDQFTMDAPRLIRDMHAAVEAGDAD
ALKLAAHTLKSSSANVGAHLLSARCREIERLARAADVTAAVELVAGTDAEFEQAHAALIAERVES
>Mature_705_residues
MMLDEIERRDVDLQAAIATAEAANSAKSDFLARMSHEIRTPMNGVLGMTELLQRTRLTSKQRKFVDTVYRSGQTLLVIID
DILDFSKIEAGKLTLERIDFNLHQLLDDVVALMEPNAQRKELELVYQRADSVPQRMRGDPVRLRQILTNLLGNAIKFTEH
GHVALSAAYSDDGRIVFKIADSGIGIAPDVAAQLFRPFQQADSSTARKYGGTGLGLVISRQLAEMMGGTLALDSTPGKGS
VFTVTTPFEPASGEVGVAAHGRPSLAGLRVLIVDDSETDRSVLLEHAIEWHLCVASAIGGDDALSQLRAAVADGAPFDLA
IIDVRMPGMDGISLVQHIRRDPALRALRIITLTAFEMMDSIQVAHELDIAARLTKPLHGNDLYTAIAAAAVHVMPPRLAS
AAPVSDEAGADPGRPLDEIAARVLLVEDNAVNQQIALAMLEDTDYRVAVADDGEQALKMLEQDAFDVVLMDCQMPVLDGF
EATRRLRRREAEAGAPRLPVIALTANALSGDRERCLAAGMDDYLGKPFRRDALLQTLARHVRGGAHVSAAASENAEAVAR
TGIETNLEASIETRPATSAAAAADPDEDANVIDRKALDALRALQRPGRPDVLTRIIDQFTMDAPRLIRDMHAAVEAGDAD
ALKLAAHTLKSSSANVGAHLLSARCREIERLARAADVTAAVELVAGTDAEFEQAHAALIAERVES

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=684, Percent_Identity=32.7485380116959, Blast_Score=301, Evalue=8e-83,
Organism=Escherichia coli, GI145693157, Length=530, Percent_Identity=32.8301886792453, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI87081816, Length=343, Percent_Identity=36.4431486880466, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI48994928, Length=347, Percent_Identity=34.2939481268012, Blast_Score=178, Evalue=8e-46,
Organism=Escherichia coli, GI1788713, Length=363, Percent_Identity=35.2617079889807, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1790436, Length=218, Percent_Identity=29.8165137614679, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1788393, Length=250, Percent_Identity=29.6, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1786783, Length=247, Percent_Identity=28.7449392712551, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786600, Length=229, Percent_Identity=28.82096069869, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1790346, Length=235, Percent_Identity=28.936170212766, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1788549, Length=227, Percent_Identity=22.4669603524229, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI87082128, Length=228, Percent_Identity=27.6315789473684, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1786912, Length=238, Percent_Identity=24.7899159663866, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1788279, Length=222, Percent_Identity=25.2252252252252, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1787894, Length=247, Percent_Identity=26.7206477732794, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1789808, Length=210, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI87082012, Length=113, Percent_Identity=33.6283185840708, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786599, Length=110, Percent_Identity=33.6363636363636, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1790300, Length=241, Percent_Identity=25.7261410788382, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787375, Length=127, Percent_Identity=33.8582677165354, Blast_Score=64, Evalue=5e-11,
Organism=Escherichia coli, GI1790437, Length=117, Percent_Identity=34.1880341880342, Blast_Score=62, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=121, Percent_Identity=41.3223140495868, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6322044, Length=75, Percent_Identity=50.6666666666667, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 75875; Mature: 75875

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50894 HPT ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLDEIERRDVDLQAAIATAEAANSAKSDFLARMSHEIRTPMNGVLGMTELLQRTRLTSK
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
QRKFVDTVYRSGQTLLVIIDDILDFSKIEAGKLTLERIDFNLHQLLDDVVALMEPNAQRK
HHHHHHHHHHCCCEEEEEEHHHHCHHHCCCCCEEHHHHCCHHHHHHHHHHHHHCCCCHHH
ELELVYQRADSVPQRMRGDPVRLRQILTNLLGNAIKFTEHGHVALSAAYSDDGRIVFKIA
HHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHCCCEEEECCCCEEEEEEECCCCEEEEEEE
DSGIGIAPDVAAQLFRPFQQADSSTARKYGGTGLGLVISRQLAEMMGGTLALDSTPGKGS
CCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCC
VFTVTTPFEPASGEVGVAAHGRPSLAGLRVLIVDDSETDRSVLLEHAIEWHLCVASAIGG
EEEEECCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
DDALSQLRAAVADGAPFDLAIIDVRMPGMDGISLVQHIRRDPALRALRIITLTAFEMMDS
HHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
IQVAHELDIAARLTKPLHGNDLYTAIAAAAVHVMPPRLASAAPVSDEAGADPGRPLDEIA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
ARVLLVEDNAVNQQIALAMLEDTDYRVAVADDGEQALKMLEQDAFDVVLMDCQMPVLDGF
HEEEEEECCCCCCHHEEEEEECCCEEEEEECCHHHHHHHHHHCCCEEEEEECCCCHHCCH
EATRRLRRREAEAGAPRLPVIALTANALSGDRERCLAAGMDDYLGKPFRRDALLQTLARH
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHCCCHHHHCCCHHHHHHHHHHHHH
VRGGAHVSAAASENAEAVARTGIETNLEASIETRPATSAAAAADPDEDANVIDRKALDAL
HCCCCCEECCCCCCHHHHHHCCCCCCCCCCEECCCCCCHHCCCCCCCCCHHHHHHHHHHH
RALQRPGRPDVLTRIIDQFTMDAPRLIRDMHAAVEAGDADALKLAAHTLKSSSANVGAHL
HHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHH
LSARCREIERLARAADVTAAVELVAGTDAEFEQAHAALIAERVES
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMLDEIERRDVDLQAAIATAEAANSAKSDFLARMSHEIRTPMNGVLGMTELLQRTRLTSK
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
QRKFVDTVYRSGQTLLVIIDDILDFSKIEAGKLTLERIDFNLHQLLDDVVALMEPNAQRK
HHHHHHHHHHCCCEEEEEEHHHHCHHHCCCCCEEHHHHCCHHHHHHHHHHHHHCCCCHHH
ELELVYQRADSVPQRMRGDPVRLRQILTNLLGNAIKFTEHGHVALSAAYSDDGRIVFKIA
HHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHCCCEEEECCCCEEEEEEECCCCEEEEEEE
DSGIGIAPDVAAQLFRPFQQADSSTARKYGGTGLGLVISRQLAEMMGGTLALDSTPGKGS
CCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCC
VFTVTTPFEPASGEVGVAAHGRPSLAGLRVLIVDDSETDRSVLLEHAIEWHLCVASAIGG
EEEEECCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
DDALSQLRAAVADGAPFDLAIIDVRMPGMDGISLVQHIRRDPALRALRIITLTAFEMMDS
HHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
IQVAHELDIAARLTKPLHGNDLYTAIAAAAVHVMPPRLASAAPVSDEAGADPGRPLDEIA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
ARVLLVEDNAVNQQIALAMLEDTDYRVAVADDGEQALKMLEQDAFDVVLMDCQMPVLDGF
HEEEEEECCCCCCHHEEEEEECCCEEEEEECCHHHHHHHHHHCCCEEEEEECCCCHHCCH
EATRRLRRREAEAGAPRLPVIALTANALSGDRERCLAAGMDDYLGKPFRRDALLQTLARH
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHCCCHHHHCCCHHHHHHHHHHHHH
VRGGAHVSAAASENAEAVARTGIETNLEASIETRPATSAAAAADPDEDANVIDRKALDAL
HCCCCCEECCCCCCHHHHHHCCCCCCCCCCEECCCCCCHHCCCCCCCCCHHHHHHHHHHH
RALQRPGRPDVLTRIIDQFTMDAPRLIRDMHAAVEAGDADALKLAAHTLKSSSANVGAHL
HHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHH
LSARCREIERLARAADVTAAVELVAGTDAEFEQAHAALIAERVES
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]