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The map label for this gene is sepA [H]

Identifier: 115351573

GI number: 115351573

Start: 1668533

End: 1669720

Strand: Direct

Name: sepA [H]

Synonym: Bamb_1520

Alternate gene names: 115351573

Gene position: 1668533-1669720 (Clockwise)

Preceding gene: 115351570

Following gene: 115351574

Centisome position: 46.91

GC content: 71.46

Gene sequence:

>1188_bases
ATGAATAACAAACGCTCCCTCGCGCGCCGCCTCCGGCTGGCGCCGGTCGCGCTCGCGGCCATGCTGGCCGTGGCCGGATG
CGGAAAGGGCGACAAGGACAAGGCGCCGGAGGCCATTCAGAACGCGACGGTCGTGACGGTGCGTCCGACGGCCGTGCCGA
TGACCGTCGAATTGCCGGGCCGGCTCGACGCGTATCGGCAAGCGCAAGTGCGCGCACGGGTCGCGGGCATCGTCACCGCG
CGCACTTACGAGGAAGGCCAGGAAGTGAAGCAGGGCGCGGTGCTGTTCCGCATCGATCCCGCGCCGCTGAAGGCCGCGCG
CGATGCCGCGCAGGGTGCGCTCGCGAAGGCGCAGGCCGCCGCGCTCGCGGCGAGCGACAAGCGCCGCCGTTACGACGACC
TGGTGCGCGACCACGCGGTCAGCGAGCGCGATCACACCGAGGCCGTCGCCGACGACACGCGGGCGAAGGCCGACGTCGCG
TCCGCGAAGGCCGAACTCGCACGCGCGCAGCTGCAGCTCGACTATGCGACCGTCACCGCGCCGATTTCGGGCCGCGCACG
ACGCGCGCTCGTCACCGAAGGCGCGCTCGTCGGCCAGGACCAGGCGACGCCGCTCACGACCGTCGAGCAGCTCGATCCGA
TCTACGTGAATTTCTCGCAGCCGGCAGCCGACGTCGACGCGTTGCGTCGCGCGGTGAAGAGCGGGCACGCGACGGGGATC
GCGCAGCACGACGTCACGGTGACGCTGCGGCGCGCCGACGGCACCGCGTATCCGCTGAAGGGCAAGCTGCTGTTCAGCGA
TCTCGCGGTCGATCCGACCACCGATACCGTCGCGATGCGCGCGCTGTTCCCCAACCCCGAACGCGAGCTGCTGCCGGGCG
CGTATGTGCGGATCGCGCTCGATGCGGCCGTCGACCAGCGGGCGATCCTGGTGCCGCGCGATGCGTTGCTGCGCACGGCC
GAGCGCACGTCGGTGCGCGTGGTCGGCACGAACGGCAAGGTGAAGGACGTCGAGGTCGTCGCCGACCAGATGAGCGGACG
CGACTGGCGCATCACGCGCGGGTTGTCGGGCGGCGAGCGCGTGATCGTCGACAACGCCGCGCAGTTCGCGCCCGATACCG
CCGTCAAGCCCGTCGAGCAGGCATCGCCGACGAAGGCCGCGCAACCGGCGGCCGCCCGTCAAACCTGA

Upstream 100 bases:

>100_bases
TTGCAGGGCAACCGGATGTATTTAATCAGTCGTGACTGATTATAATCGACCACCCTGAGCCGCCGCATTGTATCGGCGGC
GAACGATTCCGAGGTCACAC

Downstream 100 bases:

>100_bases
CCGGTTCAATAGACCAACATGGCACGTTTCTTCATCGATCGCCCCGTCTTCGCGTGGGTGATCGCACTCTTCATCATGCT
CGGCGGCGCGTTCGCGATTC

Product: periplasmic multidrug efflux lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MNNKRSLARRLRLAPVALAAMLAVAGCGKGDKDKAPEAIQNATVVTVRPTAVPMTVELPGRLDAYRQAQVRARVAGIVTA
RTYEEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAAALAASDKRRRYDDLVRDHAVSERDHTEAVADDTRAKADVA
SAKAELARAQLQLDYATVTAPISGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVDALRRAVKSGHATGI
AQHDVTVTLRRADGTAYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIALDAAVDQRAILVPRDALLRTA
ERTSVRVVGTNGKVKDVEVVADQMSGRDWRITRGLSGGERVIVDNAAQFAPDTAVKPVEQASPTKAAQPAAARQT

Sequences:

>Translated_395_residues
MNNKRSLARRLRLAPVALAAMLAVAGCGKGDKDKAPEAIQNATVVTVRPTAVPMTVELPGRLDAYRQAQVRARVAGIVTA
RTYEEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAAALAASDKRRRYDDLVRDHAVSERDHTEAVADDTRAKADVA
SAKAELARAQLQLDYATVTAPISGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVDALRRAVKSGHATGI
AQHDVTVTLRRADGTAYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIALDAAVDQRAILVPRDALLRTA
ERTSVRVVGTNGKVKDVEVVADQMSGRDWRITRGLSGGERVIVDNAAQFAPDTAVKPVEQASPTKAAQPAAARQT
>Mature_395_residues
MNNKRSLARRLRLAPVALAAMLAVAGCGKGDKDKAPEAIQNATVVTVRPTAVPMTVELPGRLDAYRQAQVRARVAGIVTA
RTYEEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAAALAASDKRRRYDDLVRDHAVSERDHTEAVADDTRAKADVA
SAKAELARAQLQLDYATVTAPISGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVDALRRAVKSGHATGI
AQHDVTVTLRRADGTAYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIALDAAVDQRAILVPRDALLRTA
ERTSVRVVGTNGKVKDVEVVADQMSGRDWRITRGLSGGERVIVDNAAQFAPDTAVKPVEQASPTKAAQPAAARQT

Specific function: Probable periplasmic linker protein component of the sepABC efflux pump with unknown specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=372, Percent_Identity=40.8602150537634, Blast_Score=244, Evalue=7e-66,
Organism=Escherichia coli, GI1786668, Length=387, Percent_Identity=40.0516795865633, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1789929, Length=372, Percent_Identity=36.8279569892473, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI87082036, Length=374, Percent_Identity=31.5508021390374, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42087; Mature: 42087

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKRSLARRLRLAPVALAAMLAVAGCGKGDKDKAPEAIQNATVVTVRPTAVPMTVELPG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCEEEEEECCCEEEEEECCC
RLDAYRQAQVRARVAGIVTARTYEEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAA
CHHHHHHHHHHHHHHHHEEEHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
ALAASDKRRRYDDLVRDHAVSERDHTEAVADDTRAKADVASAKAELARAQLQLDYATVTA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEE
PISGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVDALRRAVKSGHATGI
CCCCCHHHEEEECCCEECCCCCCCCCHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCE
AQHDVTVTLRRADGTAYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIAL
EECCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHCCCEEEEEEE
DAAVDQRAILVPRDALLRTAERTSVRVVGTNGKVKDVEVVADQMSGRDWRITRGLSGGER
ECCCCCCEEECCHHHHHHHHCCCEEEEEECCCCEEHHHHHHHHHCCCCEEEECCCCCCCE
VIVDNAAQFAPDTAVKPVEQASPTKAAQPAAARQT
EEEECCHHHCCCCCCCCHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNNKRSLARRLRLAPVALAAMLAVAGCGKGDKDKAPEAIQNATVVTVRPTAVPMTVELPG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCEEEEEECCCEEEEEECCC
RLDAYRQAQVRARVAGIVTARTYEEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAA
CHHHHHHHHHHHHHHHHEEEHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
ALAASDKRRRYDDLVRDHAVSERDHTEAVADDTRAKADVASAKAELARAQLQLDYATVTA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEE
PISGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVDALRRAVKSGHATGI
CCCCCHHHEEEECCCEECCCCCCCCCHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCE
AQHDVTVTLRRADGTAYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIAL
EECCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHCCCEEEEEEE
DAAVDQRAILVPRDALLRTAERTSVRVVGTNGKVKDVEVVADQMSGRDWRITRGLSGGER
ECCCCCCEEECCHHHHHHHHCCCEEEEEECCCCEEHHHHHHHHHCCCCEEEECCCCCCCE
VIVDNAAQFAPDTAVKPVEQASPTKAAQPAAARQT
EEEECCHHHCCCCCCCCHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9037074 [H]