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The map label for this gene is dusA [H]

Identifier: 115351522

GI number: 115351522

Start: 1615694

End: 1616728

Strand: Direct

Name: dusA [H]

Synonym: Bamb_1469

Alternate gene names: 115351522

Gene position: 1615694-1616728 (Clockwise)

Preceding gene: 115351521

Following gene: 115351525

Centisome position: 45.43

GC content: 63.96

Gene sequence:

>1035_bases
ATGTCCGCACCGTCCTCGCTTCCTCCCCGCCGCGTTTCAGTGGCGCCCATGCTCGACTGGACCGACCGCCATTGCCGGTC
GTTCCATCGCACGCTGACGCGCGACACGTGGCTGTATACGGAAATGATCACCACGGGCGCGCTGCTGTTCGGCGACGCGC
AGCGGCATCTGGCGTTCACGCCGAACGAATCGCCGGTCGCGCTGCAGTTGGGCGGCAGCGAGCGGGACGATCTCGCGCGC
GCCGCGAAACTCGGCGAGCAATGGGGCTATGACGAGATCAACCTGAATTGCGGCTGTCCGTCTGAGCGGGTGCAGCGTGG
CGCATTCGGCGCGTGCTTGATGAACGAGCCGCAGCTCGTCGCCGACTGCGTGAAGGCGATGCGCGATGCGGTGTCGGTGC
CGGTCACGGTCAAGCACCGGATCGGCGTCGACGCGGTCGAGGATTATGCGTTCGTGCGCGACTTCGTCGGCAAGGTGGCG
GAAGCGGGCTGCGAGACGTTCGTCGTGCACGCGCGCAATGCGATCCTGAAGGGGCTGTCGCCGAAGGAGAACCGCGAGAT
CCCGCCGCTCAAGTACGACTATGCGTATCAACTGAAGCGCGATTTTCCGTCGCTGGAGATCGTGATCAACGGCGGGATCA
AGACGCTCGACGAAGTCGCGCAGCATCTCGAGCATGTCGACGGCGTGATGCTTGGCCGCGAGGCTTATCACAACCCGTAT
GTACTCGCGGGCGTCGATGCGCGCTTCTATGGTTCGACCGCGGCGGTGCCGACGCGCGAAGAGGCCGAGGCGAAACTGAT
CGAATACTGCGCGGCGGAATTGAAGCGCGGTACGTATCTCGGCGCGATCGTCCGGCACGCACTCGGGCTGTATCGCGGCG
TCGGCGGTGCACGCGGCTGGCGTCGCGTGCTGTCGGACAACAAGAGGCTCGCGCGCGGCGATCTGGCCGTGTTCGACGAG
GCGCGCACGCATCTGAACGACGCGATCGAAAATTTTGAAAAAAATGCTTTGCAAGACGGAAAAGTGTTCGTATAA

Upstream 100 bases:

>100_bases
TCGGCGAGGGCGGGCCGGTTTAGTCAAGCTGCCGGGCTCGGCTGGTCACGTTGCGGTAAAATGCCGGGTTGTTCCCGCGC
CGCTTGCTGCCCCGAATTCC

Downstream 100 bases:

>100_bases
TCTTGTTCTTCGCTGCTGAACACGGACGAAACGAAACAGCGAAGAAACAAAGCAGTATCAGTGGTGGCTGTAGCTCAGTT
GGTAGAGTCCAGGATTGTGA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSAPSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRDTWLYTEMITTGALLFGDAQRHLAFTPNESPVALQLGGSERDDLAR
AAKLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVKAMRDAVSVPVTVKHRIGVDAVEDYAFVRDFVGKVA
EAGCETFVVHARNAILKGLSPKENREIPPLKYDYAYQLKRDFPSLEIVINGGIKTLDEVAQHLEHVDGVMLGREAYHNPY
VLAGVDARFYGSTAAVPTREEAEAKLIEYCAAELKRGTYLGAIVRHALGLYRGVGGARGWRRVLSDNKRLARGDLAVFDE
ARTHLNDAIENFEKNALQDGKVFV

Sequences:

>Translated_344_residues
MSAPSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRDTWLYTEMITTGALLFGDAQRHLAFTPNESPVALQLGGSERDDLAR
AAKLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVKAMRDAVSVPVTVKHRIGVDAVEDYAFVRDFVGKVA
EAGCETFVVHARNAILKGLSPKENREIPPLKYDYAYQLKRDFPSLEIVINGGIKTLDEVAQHLEHVDGVMLGREAYHNPY
VLAGVDARFYGSTAAVPTREEAEAKLIEYCAAELKRGTYLGAIVRHALGLYRGVGGARGWRRVLSDNKRLARGDLAVFDE
ARTHLNDAIENFEKNALQDGKVFV
>Mature_343_residues
SAPSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRDTWLYTEMITTGALLFGDAQRHLAFTPNESPVALQLGGSERDDLARA
AKLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVKAMRDAVSVPVTVKHRIGVDAVEDYAFVRDFVGKVAE
AGCETFVVHARNAILKGLSPKENREIPPLKYDYAYQLKRDFPSLEIVINGGIKTLDEVAQHLEHVDGVMLGREAYHNPYV
LAGVDARFYGSTAAVPTREEAEAKLIEYCAAELKRGTYLGAIVRHALGLYRGVGGARGWRRVLSDNKRLARGDLAVFDEA
RTHLNDAIENFEKNALQDGKVFV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=237, Percent_Identity=30.379746835443, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI40807366, Length=245, Percent_Identity=26.9387755102041, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI145693211, Length=312, Percent_Identity=59.6153846153846, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1788462, Length=188, Percent_Identity=31.9148936170213, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789660, Length=297, Percent_Identity=23.2323232323232, Blast_Score=72, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI25144369, Length=213, Percent_Identity=29.1079812206573, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=179, Percent_Identity=29.608938547486, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=241, Percent_Identity=26.5560165975104, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24580595, Length=296, Percent_Identity=27.027027027027, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19920448, Length=296, Percent_Identity=27.027027027027, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38145; Mature: 38014

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRDTWLYTEMITTGALLFGDAQRHLAFT
CCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEC
PNESPVALQLGGSERDDLARAAKLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPQLV
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCHHHCCCCHHH
ADCVKAMRDAVSVPVTVKHRIGVDAVEDYAFVRDFVGKVAEAGCETFVVHARNAILKGLS
HHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
PKENREIPPLKYDYAYQLKRDFPSLEIVINGGIKTLDEVAQHLEHVDGVMLGREAYHNPY
CCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VLAGVDARFYGSTAAVPTREEAEAKLIEYCAAELKRGTYLGAIVRHALGLYRGVGGARGW
EEEECCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHH
RRVLSDNKRLARGDLAVFDEARTHLNDAIENFEKNALQDGKVFV
HHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SAPSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRDTWLYTEMITTGALLFGDAQRHLAFT
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEC
PNESPVALQLGGSERDDLARAAKLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPQLV
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCHHHCCCCHHH
ADCVKAMRDAVSVPVTVKHRIGVDAVEDYAFVRDFVGKVAEAGCETFVVHARNAILKGLS
HHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
PKENREIPPLKYDYAYQLKRDFPSLEIVINGGIKTLDEVAQHLEHVDGVMLGREAYHNPY
CCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VLAGVDARFYGSTAAVPTREEAEAKLIEYCAAELKRGTYLGAIVRHALGLYRGVGGARGW
EEEECCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHH
RRVLSDNKRLARGDLAVFDEARTHLNDAIENFEKNALQDGKVFV
HHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]