BacMap

Definition	Burkholderia ambifaria AMMD chromosome chromosome 1, complete sequence.
Accession	NC_008390
Length	3,556,545

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The map label for this gene is ygfF [C]

Identifier: 115351351

GI number: 115351351

Start: 1422777

End: 1423523

Strand: Direct

Name: ygfF [C]

Synonym: Bamb_1298

Alternate gene names: 115351351

Gene position: 1422777-1423523 (Clockwise)

Preceding gene: 115351350

Following gene: 115351357

Centisome position: 40.0

GC content: 67.47

Gene sequence:

>747_bases
ATGAGCAAGCTGACAAACAAGGTTGCGATCGTCACGGGCGGGTCCAAGGGCATCGGCGCCGCGATCGCGAAGGCGCTGGC
CGCCGAAGGTGCGTCGGTCGTCGTCAACTACGCGAGCAGCAAGGCCGGTGCCGATGCCGTCGTGAGCGCGATCGTCGAAG
CAGGCGGCCGCGCGGTCGCGGTGGGCGGCGACGTGTCGAAGGCAGCGGACGCGCAGCGCATCGTCGATGCCGCGATCGAG
AACTATGGCCGTCTCGACGTGCTGGTCAACAATTCCGGCGTCTACGAATTCGCGCCGATCGAGGCGATCACCGAAGAGCA
CTATCGCCGGCAGTTCGATACGAACGTGTTCGGCGTGCTGCTGACGACGCAGGCCGCCGTCAAGCATCTCGGCGAAGGCG
CGAGCATCATCAACATCAGCTCGGTCGTGACGAGCATCACGCCGCCCGCGAGCGCCGTGTACAGCGGCACCAAGGGCGCC
GTCGACGCGATCACCGGCGTGCTCGCGCTCGAGCTCGGCCCGCGCAAGATTCGCGTGAATGCGATCAACCCGGGGATGAT
CGTGACCGAAGGCACGCACAGCGCGGGCATCATCGGTTCGGATCTCGAAACGCAGGTGCGCAACGATACGCCGCTTGGCC
GCCTCGGCGAACCGGGCGACATCGCGTCGGTCGCCGTGTTCCTCGCGTCGGACGATGCGCGCTGGCTGACCGGCGAGCGC
CTCGTCGCGAGCGGCGGGCTGCACTGA

Upstream 100 bases:

>100_bases
TGCGGCGCAGCACCTTACATTGCCAGTCATGCCGGTGCGCCGCCACCACGCGGCAACCGGCTCCCATCCATTGAAAGTCT
TCCGACTGAGGAATGAAATC

Downstream 100 bases:

>100_bases
CGCGCCAGGTCGGCGCGCGCCGCTACGCGATCATCGCGAGCGCGTTCGCGCCGACATGCCAGCGCAGGCTCTCGACGACG
AGCGCGTGATCGTGTTCGTC

Product: short-chain dehydrogenase/reductase SDR

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: ORFC [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MSKLTNKVAIVTGGSKGIGAAIAKALAAEGASVVVNYASSKAGADAVVSAIVEAGGRAVAVGGDVSKAADAQRIVDAAIE
NYGRLDVLVNNSGVYEFAPIEAITEEHYRRQFDTNVFGVLLTTQAAVKHLGEGASIINISSVVTSITPPASAVYSGTKGA
VDAITGVLALELGPRKIRVNAINPGMIVTEGTHSAGIIGSDLETQVRNDTPLGRLGEPGDIASVAVFLASDDARWLTGER
LVASGGLH

Sequences:

>Translated_248_residues
MSKLTNKVAIVTGGSKGIGAAIAKALAAEGASVVVNYASSKAGADAVVSAIVEAGGRAVAVGGDVSKAADAQRIVDAAIE
NYGRLDVLVNNSGVYEFAPIEAITEEHYRRQFDTNVFGVLLTTQAAVKHLGEGASIINISSVVTSITPPASAVYSGTKGA
VDAITGVLALELGPRKIRVNAINPGMIVTEGTHSAGIIGSDLETQVRNDTPLGRLGEPGDIASVAVFLASDDARWLTGER
LVASGGLH
>Mature_247_residues
SKLTNKVAIVTGGSKGIGAAIAKALAAEGASVVVNYASSKAGADAVVSAIVEAGGRAVAVGGDVSKAADAQRIVDAAIEN
YGRLDVLVNNSGVYEFAPIEAITEEHYRRQFDTNVFGVLLTTQAAVKHLGEGASIINISSVVTSITPPASAVYSGTKGAV
DAITGVLALELGPRKIRVNAINPGMIVTEGTHSAGIIGSDLETQVRNDTPLGRLGEPGDIASVAVFLASDDARWLTGERL
VASGGLH

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=246, Percent_Identity=36.5853658536585, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI40254992, Length=246, Percent_Identity=34.5528455284553, Blast_Score=121, Evalue=6e-28,
Organism=Homo sapiens, GI126723750, Length=253, Percent_Identity=33.596837944664, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI7705925, Length=247, Percent_Identity=34.0080971659919, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI59889578, Length=219, Percent_Identity=35.1598173515982, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI5031737, Length=248, Percent_Identity=33.8709677419355, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI15277342, Length=257, Percent_Identity=31.9066147859922, Blast_Score=104, Evalue=6e-23,
Organism=Homo sapiens, GI10190704, Length=247, Percent_Identity=33.1983805668016, Blast_Score=104, Evalue=6e-23,
Organism=Homo sapiens, GI19923817, Length=251, Percent_Identity=29.4820717131474, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI126723191, Length=186, Percent_Identity=37.0967741935484, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI33667109, Length=192, Percent_Identity=34.8958333333333, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI31542939, Length=261, Percent_Identity=29.1187739463602, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI4503301, Length=251, Percent_Identity=29.0836653386454, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI4758504, Length=248, Percent_Identity=28.2258064516129, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI109715829, Length=249, Percent_Identity=27.3092369477912, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI66933014, Length=253, Percent_Identity=30.0395256916996, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4504505, Length=192, Percent_Identity=30.2083333333333, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI7706318, Length=193, Percent_Identity=26.9430051813472, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI224922801, Length=169, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI2367175, Length=246, Percent_Identity=36.9918699186992, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1788459, Length=244, Percent_Identity=34.4262295081967, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787905, Length=246, Percent_Identity=35.3658536585366, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1789378, Length=248, Percent_Identity=35.8870967741936, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1787335, Length=244, Percent_Identity=33.1967213114754, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI87082100, Length=254, Percent_Identity=32.6771653543307, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1790717, Length=253, Percent_Identity=30.0395256916996, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789208, Length=252, Percent_Identity=29.7619047619048, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082160, Length=250, Percent_Identity=32, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI2367365, Length=239, Percent_Identity=30.9623430962343, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1786812, Length=252, Percent_Identity=26.984126984127, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788891, Length=265, Percent_Identity=29.811320754717, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1789057, Length=254, Percent_Identity=25.9842519685039, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787526, Length=249, Percent_Identity=28.5140562248996, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1786701, Length=209, Percent_Identity=31.5789473684211, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=252, Percent_Identity=34.1269841269841, Blast_Score=131, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17560676, Length=255, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71994604, Length=253, Percent_Identity=33.9920948616601, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560332, Length=263, Percent_Identity=34.2205323193916, Blast_Score=113, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17544670, Length=266, Percent_Identity=29.3233082706767, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17562910, Length=263, Percent_Identity=31.9391634980989, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25147288, Length=248, Percent_Identity=32.258064516129, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17562906, Length=270, Percent_Identity=29.2592592592593, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17555706, Length=247, Percent_Identity=33.1983805668016, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI115534694, Length=247, Percent_Identity=30.7692307692308, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17560150, Length=268, Percent_Identity=30.9701492537313, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71994600, Length=249, Percent_Identity=30.9236947791165, Blast_Score=100, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17538486, Length=263, Percent_Identity=29.6577946768061, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17559104, Length=265, Percent_Identity=33.2075471698113, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17562990, Length=267, Percent_Identity=30.7116104868914, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI72000259, Length=263, Percent_Identity=31.1787072243346, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17562908, Length=269, Percent_Identity=28.6245353159851, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17562904, Length=269, Percent_Identity=28.6245353159851, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17565030, Length=266, Percent_Identity=31.203007518797, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI193204405, Length=263, Percent_Identity=29.277566539924, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17531453, Length=269, Percent_Identity=29.368029739777, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17551412, Length=265, Percent_Identity=30.188679245283, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17538480, Length=262, Percent_Identity=30.1526717557252, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17563726, Length=263, Percent_Identity=29.6577946768061, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17560220, Length=263, Percent_Identity=28.5171102661597, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17508651, Length=249, Percent_Identity=29.3172690763052, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17561272, Length=252, Percent_Identity=31.3492063492063, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17568967, Length=192, Percent_Identity=32.2916666666667, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17536025, Length=249, Percent_Identity=26.9076305220884, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538182, Length=260, Percent_Identity=29.2307692307692, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17536651, Length=247, Percent_Identity=26.7206477732794, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71982365, Length=192, Percent_Identity=28.125, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=246, Percent_Identity=28.0487804878049, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322861, Length=192, Percent_Identity=32.2916666666667, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322226, Length=189, Percent_Identity=27.5132275132275, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571526, Length=258, Percent_Identity=40.6976744186046, Blast_Score=150, Evalue=7e-37,
Organism=Drosophila melanogaster, GI23397609, Length=253, Percent_Identity=37.9446640316205, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21357041, Length=258, Percent_Identity=36.8217054263566, Blast_Score=137, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24644339, Length=249, Percent_Identity=37.3493975903614, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24643142, Length=252, Percent_Identity=32.1428571428571, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24639444, Length=250, Percent_Identity=33.6, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21355319, Length=256, Percent_Identity=32.421875, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24644337, Length=255, Percent_Identity=31.7647058823529, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24641232, Length=203, Percent_Identity=30.5418719211823, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281364892, Length=191, Percent_Identity=31.413612565445, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI17737361, Length=260, Percent_Identity=27.6923076923077, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI281364494, Length=240, Percent_Identity=30.8333333333333, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI116007236, Length=203, Percent_Identity=29.5566502463054, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI18859837, Length=132, Percent_Identity=34.8484848484849, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24580925, Length=204, Percent_Identity=26.9607843137255, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24663911, Length=202, Percent_Identity=27.7227722772277, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI116007234, Length=203, Percent_Identity=31.0344827586207, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24641388, Length=217, Percent_Identity=33.1797235023041, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648693, Length=204, Percent_Identity=30.8823529411765, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI21355889, Length=204, Percent_Identity=30.8823529411765, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24656154, Length=221, Percent_Identity=28.9592760180996, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI19922628, Length=221, Percent_Identity=28.9592760180996, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI116007238, Length=190, Percent_Identity=28.9473684210526, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 25160; Mature: 25029

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLTNKVAIVTGGSKGIGAAIAKALAAEGASVVVNYASSKAGADAVVSAIVEAGGRAVA
CCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEE
VGGDVSKAADAQRIVDAAIENYGRLDVLVNNSGVYEFAPIEAITEEHYRRQFDTNVFGVL
ECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHCCCCEEHE
LTTQAAVKHLGEGASIINISSVVTSITPPASAVYSGTKGAVDAITGVLALELGPRKIRVN
EHHHHHHHHHCCCCCEEEHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHEEECCCEEEEE
AINPGMIVTEGTHSAGIIGSDLETQVRNDTPLGRLGEPGDIASVAVFLASDDARWLTGER
EECCCEEEECCCCCCCEECCCCHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCEECCCE
LVASGGLH
EECCCCCC
>Mature Secondary Structure 
SKLTNKVAIVTGGSKGIGAAIAKALAAEGASVVVNYASSKAGADAVVSAIVEAGGRAVA
CCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEE
VGGDVSKAADAQRIVDAAIENYGRLDVLVNNSGVYEFAPIEAITEEHYRRQFDTNVFGVL
ECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHCCCCEEHE
LTTQAAVKHLGEGASIINISSVVTSITPPASAVYSGTKGAVDAITGVLALELGPRKIRVN
EHHHHHHHHHCCCCCEEEHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHEEECCCEEEEE
AINPGMIVTEGTHSAGIIGSDLETQVRNDTPLGRLGEPGDIASVAVFLASDDARWLTGER
EECCCEEEECCCCCCCEECCCCHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCEECCCE
LVASGGLH
EECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8868443 [H]