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The map label for this gene is mltD [H]

Identifier: 115351218

GI number: 115351218

Start: 1272540

End: 1274129

Strand: Direct

Name: mltD [H]

Synonym: Bamb_1165

Alternate gene names: 115351218

Gene position: 1272540-1274129 (Clockwise)

Preceding gene: 115351217

Following gene: 115351219

Centisome position: 35.78

GC content: 67.3

Gene sequence:

>1590_bases
ATGCGACTTATATTGAGTGCGATGGTGGTCCTGCTACTCGCCGCTTGTGCGAGCCAGGCTCCTGTCGCCAACAACGCCGC
CGATTCGCAGGCGACGTCCAACTACCTCCGTAAATCAGCCACCGCCAAAGAAACTGTCGACGTCGACAAGCAATCCGTCG
GCGACCTGACCAGTGCGGACTCCGATCTCTGGGGCCGCATCCGTCGCGGCTTCCAGATGCCCGACCTGCAGAGCGACCTC
GTCGACATGCAGACCACGTGGTACACGCAGCGTCCCGATTACGTGCAGCGCATGACCGAGCGCTCGCAGAAGTACCTGTA
TCACATCGTCGAGGAACTCGAGGCGCGCCACATGCCGACCGAGCTCGCGCTGCTGCCGTTCATCGAATCCGCGTACAACC
CGCAGGCGCTGTCGGTCGCGAAGGCGGCCGGCATGTGGCAGTTCATGCCGGGCACGGGCCGCACGTACAACCTGAAGCGC
AACATGTGGCAGGACGAGCGTCGCGACGTGCTCGCGTCGACGAGCGCCGCGCTCGACTACCTGTCGCGCCTGCATGACAT
GTTCGGCGACTGGTATCTGGCGCTGGCCGCGTACAACTGGGGCGAGGGCAACGTGCAGCGCGCGATCGCGCGCAACCAGG
CGGCCGGGCTGCCGACCGACTACCAGAGCCTGCGGATGCCGAACGAGACGCGCAACTACGTGCCGAAGCTGCAGGCGGTG
AAGAACATCATCGCGAGTCCGCAGCAGTATGGCCTCGTGCTGCCTGACATCCCGAACCACCCGTATTTCGTGACGGTCAC
GACGTCGCGCGACATCGACGTGGCGGTGGCCGCGAAGCTCGCGAACCTGTCGCTCGACGAATTCCGCTCGCTGAACCCGT
CGTTCGCGAAGCCGGTGATCCTCGGCGCGACCGAGCCGCAGATCCTGCTGCCGTTCGACAACGCGGCGGCGTTCGAGAAG
AACCTGAAGGCACACAACGGCCAGCTGTCGTCGTGGACCACCTACACGGTCAGCGAGCGCGCGCGTCCGGCCGCGATCGC
CGAGAAGATCGGTGTCGACGCCGATACGCTGATGTCGATCAACAAGATTCCGGCCGGCATGCGCCTGAAGCCGGGTTCGA
CGATCGTCGTGCCGCGTGGCGATGACGACGACGAGGACATCAGCGCGGACGTCGCCGAAAATGGCGTGCTCGCGATGGAG
CCCGACGTGCCCGACACGCGCAAGATGCTGATCCGCGTGCGCCGCAAGCAGTCGATGGCCGCACTCGCCGGCCGCTACGG
CGTGTCGGTCGGCCAGCTGAAGGCATGGAACCGCACGCACCGCGATATGGTGATGCCGGGCCAGGCGCTCGTGCTGCACG
TGCCGGTCGGCCGTGCGGTGCCGGCCGAGCCGGGTCCGGAGCGGATCGCGACGTCGGCGGCCGGCGCGCATATCGAGCGC
GCGAGCCTGTCCGTCGGCGGCAAGTCGCGTGGCGCGAAGCACGCCGCCGCGAAGCCGGCGGCCCGCGCGGCCAAGGCGGC
CCCCGCGAGGGCGGCCCCCGCGAAGGCGGCGCCGGCCAAGGTTGCTGCCAGCAAGAGCAAGAAGAAATAA

Upstream 100 bases:

>100_bases
AATGTAGGATTTCGCTGAGTTTTCGGTGTTTTTATTGACGTGAAGCACGCACTTCCGTAGTATCGCCTGCAATTTCCAGC
CGTCGGAAGCCGAGATTTTC

Downstream 100 bases:

>100_bases
CGCGGTCGCGATCGCAACGCGGCGGTCCGTACGGGCCGTGCGATTGCGTTATCATCCGACGAAACGCGAGGCAACGCCGT
CACCGAGGTGACGGCGTTTT

Product: lytic transglycosylase catalytic subunit

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MRLILSAMVVLLLAACASQAPVANNAADSQATSNYLRKSATAKETVDVDKQSVGDLTSADSDLWGRIRRGFQMPDLQSDL
VDMQTTWYTQRPDYVQRMTERSQKYLYHIVEELEARHMPTELALLPFIESAYNPQALSVAKAAGMWQFMPGTGRTYNLKR
NMWQDERRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQAV
KNIIASPQQYGLVLPDIPNHPYFVTVTTSRDIDVAVAAKLANLSLDEFRSLNPSFAKPVILGATEPQILLPFDNAAAFEK
NLKAHNGQLSSWTTYTVSERARPAAIAEKIGVDADTLMSINKIPAGMRLKPGSTIVVPRGDDDDEDISADVAENGVLAME
PDVPDTRKMLIRVRRKQSMAALAGRYGVSVGQLKAWNRTHRDMVMPGQALVLHVPVGRAVPAEPGPERIATSAAGAHIER
ASLSVGGKSRGAKHAAAKPAARAAKAAPARAAPAKAAPAKVAASKSKKK

Sequences:

>Translated_529_residues
MRLILSAMVVLLLAACASQAPVANNAADSQATSNYLRKSATAKETVDVDKQSVGDLTSADSDLWGRIRRGFQMPDLQSDL
VDMQTTWYTQRPDYVQRMTERSQKYLYHIVEELEARHMPTELALLPFIESAYNPQALSVAKAAGMWQFMPGTGRTYNLKR
NMWQDERRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQAV
KNIIASPQQYGLVLPDIPNHPYFVTVTTSRDIDVAVAAKLANLSLDEFRSLNPSFAKPVILGATEPQILLPFDNAAAFEK
NLKAHNGQLSSWTTYTVSERARPAAIAEKIGVDADTLMSINKIPAGMRLKPGSTIVVPRGDDDDEDISADVAENGVLAME
PDVPDTRKMLIRVRRKQSMAALAGRYGVSVGQLKAWNRTHRDMVMPGQALVLHVPVGRAVPAEPGPERIATSAAGAHIER
ASLSVGGKSRGAKHAAAKPAARAAKAAPARAAPAKAAPAKVAASKSKKK
>Mature_529_residues
MRLILSAMVVLLLAACASQAPVANNAADSQATSNYLRKSATAKETVDVDKQSVGDLTSADSDLWGRIRRGFQMPDLQSDL
VDMQTTWYTQRPDYVQRMTERSQKYLYHIVEELEARHMPTELALLPFIESAYNPQALSVAKAAGMWQFMPGTGRTYNLKR
NMWQDERRDVLASTSAALDYLSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQAV
KNIIASPQQYGLVLPDIPNHPYFVTVTTSRDIDVAVAAKLANLSLDEFRSLNPSFAKPVILGATEPQILLPFDNAAAFEK
NLKAHNGQLSSWTTYTVSERARPAAIAEKIGVDADTLMSINKIPAGMRLKPGSTIVVPRGDDDDEDISADVAENGVLAME
PDVPDTRKMLIRVRRKQSMAALAGRYGVSVGQLKAWNRTHRDMVMPGQALVLHVPVGRAVPAEPGPERIATSAAGAHIER
ASLSVGGKSRGAKHAAAKPAARAAKAAPARAAPAKAAPAKVAASKSKKK

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=458, Percent_Identity=34.4978165938865, Blast_Score=239, Evalue=4e-64,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 57620; Mature: 57620

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLILSAMVVLLLAACASQAPVANNAADSQATSNYLRKSATAKETVDVDKQSVGDLTSAD
CHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCHHHHCCCCCCC
SDLWGRIRRGFQMPDLQSDLVDMQTTWYTQRPDYVQRMTERSQKYLYHIVEELEARHMPT
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ELALLPFIESAYNPQALSVAKAAGMWQFMPGTGRTYNLKRNMWQDERRDVLASTSAALDY
HHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHH
LSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQAV
HHHHHHHHHHHEEHHEEECCCCCHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHH
KNIIASPQQYGLVLPDIPNHPYFVTVTTSRDIDVAVAAKLANLSLDEFRSLNPSFAKPVI
HHHHCCHHHCCEECCCCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCEE
LGATEPQILLPFDNAAAFEKNLKAHNGQLSSWTTYTVSERARPAAIAEKIGVDADTLMSI
EECCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHH
NKIPAGMRLKPGSTIVVPRGDDDDEDISADVAENGVLAMEPDVPDTRKMLIRVRRKQSMA
HCCCCCCEECCCCEEEEECCCCCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHH
ALAGRYGVSVGQLKAWNRTHRDMVMPGQALVLHVPVGRAVPAEPGPERIATSAAGAHIER
HHHHHHCCCHHHHHHCCCCCCCEECCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEH
ASLSVGGKSRGAKHAAAKPAARAAKAAPARAAPAKAAPAKVAASKSKKK
HHEECCCCCCCCCHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MRLILSAMVVLLLAACASQAPVANNAADSQATSNYLRKSATAKETVDVDKQSVGDLTSAD
CHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCHHHHCCCCCCC
SDLWGRIRRGFQMPDLQSDLVDMQTTWYTQRPDYVQRMTERSQKYLYHIVEELEARHMPT
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ELALLPFIESAYNPQALSVAKAAGMWQFMPGTGRTYNLKRNMWQDERRDVLASTSAALDY
HHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHH
LSRLHDMFGDWYLALAAYNWGEGNVQRAIARNQAAGLPTDYQSLRMPNETRNYVPKLQAV
HHHHHHHHHHHEEHHEEECCCCCHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHH
KNIIASPQQYGLVLPDIPNHPYFVTVTTSRDIDVAVAAKLANLSLDEFRSLNPSFAKPVI
HHHHCCHHHCCEECCCCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCEE
LGATEPQILLPFDNAAAFEKNLKAHNGQLSSWTTYTVSERARPAAIAEKIGVDADTLMSI
EECCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHH
NKIPAGMRLKPGSTIVVPRGDDDDEDISADVAENGVLAMEPDVPDTRKMLIRVRRKQSMA
HCCCCCCEECCCCEEEEECCCCCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHH
ALAGRYGVSVGQLKAWNRTHRDMVMPGQALVLHVPVGRAVPAEPGPERIATSAAGAHIER
HHHHHHCCCHHHHHHCCCCCCCEECCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEH
ASLSVGGKSRGAKHAAAKPAARAAKAAPARAAPAKAAPAKVAASKSKKK
HHEECCCCCCCCCHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]