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The map label for this gene is gmd [H]

Identifier: 115350813

GI number: 115350813

Start: 843522

End: 844562

Strand: Direct

Name: gmd [H]

Synonym: Bamb_0759

Alternate gene names: 115350813

Gene position: 843522-844562 (Clockwise)

Preceding gene: 115350812

Following gene: 115350814

Centisome position: 23.72

GC content: 60.04

Gene sequence:

>1041_bases
ATGTCTAAGAAAACCTCCATCATCACTGGCATCACCGGACAAGACGGTGCTTATCTGGCCGAGTTGCTGCTCGACAAGGG
TTACACGGTTTACGGTACCTACCGCCGCACCAGCTCGGTGAACTTCTGGCGCATCGAGGAACTGGGCATTGCCAAGCACC
CGAACCTCCATCTTGTCGAATACGACCTGACCGATCTGTCGGCGAGCATTCGCTTGCTTCAGACGACCGGGGCCACGGAG
GTTTACAACCTCGCGGCGCAGAGTTTCGTCGGCGTGTCGTTCGACCAGCCGGTCACGACCGCGGAGATCACCGGCGTCGG
TCCGTTGAACCTGCTCGAGGCGATTCGCATCGTCAATCCGAAGATCCGCTTCTATCAGGCAAGCACTTCCGAAATGTTCG
GCAAGGTCCAGGCGATCCCGCAGATCGAATCGACGCCGTTCTACCCGCGCAGCCCGTACGGCGTGGCCAAGCTGTATGCG
CACTGGATCACGGTGAACTATCGCGAGAGCTACGACATCTTCGGCTGCAGCGGGATCTTGTTCAACCACGAGTCGCCGCT
GCGCGGCCGCGAATTCGTCACACGCAAGATCACCGACTCCGTTGCCAAGATCAAGCTCGGGCAACTCGACGTCCTCGAAC
TGGGCAACATGGATGCCAAGCGCGACTGGGGCTTCGCGAAGGAATATGTCGAAGGCATGTGGCGCATGCTGCAGGCAGAC
GAACCGGACACGTTCGTGCTGGCCACGAACCGTACCGAGACCGTGCGCGATTTCGTGCGCATGGCGTTCAAGGCGGCTGG
TGTCGACCTCGAGTTCAAGGGCAGTGACGAGCAGGAAATTGCCGTCGATGTCGCCACCGGCAAGACGCTGGTCCGCGTGA
ATCCGAAGTTCCATCGGCCGGCCGAAGTCGATCTGCTGATCGGGAATCCCGAGAAGGCGAAGCAGAAGCTGGGCTGGGAG
CCGAAGACGACGCTCGAGGAGCTGTGTGCGATGATGGTTGAAGCGGACTTGCGACGAAATGAGCGCGGCTTCTCGTTCTG
A

Upstream 100 bases:

>100_bases
GGCGTCTCGCGGTATGCCGTTCATGACTTGGGCGCAGAGTGCTCGCCAGTTGGTAAAAATTCTCACGCGCGACGAAGCGT
ATTCTTAAAGTAGGACCATT

Downstream 100 bases:

>100_bases
TGTCCCGCGCGCGCTGGTAACGGGCCTCGGCGGCTTTACCGGCGACTATCTCGCGGAGTCGCTGCGGGCGGCCGGGTATC
GCGTGTTCGGCACGACACAC

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MSKKTSIITGITGQDGAYLAELLLDKGYTVYGTYRRTSSVNFWRIEELGIAKHPNLHLVEYDLTDLSASIRLLQTTGATE
VYNLAAQSFVGVSFDQPVTTAEITGVGPLNLLEAIRIVNPKIRFYQASTSEMFGKVQAIPQIESTPFYPRSPYGVAKLYA
HWITVNYRESYDIFGCSGILFNHESPLRGREFVTRKITDSVAKIKLGQLDVLELGNMDAKRDWGFAKEYVEGMWRMLQAD
EPDTFVLATNRTETVRDFVRMAFKAAGVDLEFKGSDEQEIAVDVATGKTLVRVNPKFHRPAEVDLLIGNPEKAKQKLGWE
PKTTLEELCAMMVEADLRRNERGFSF

Sequences:

>Translated_346_residues
MSKKTSIITGITGQDGAYLAELLLDKGYTVYGTYRRTSSVNFWRIEELGIAKHPNLHLVEYDLTDLSASIRLLQTTGATE
VYNLAAQSFVGVSFDQPVTTAEITGVGPLNLLEAIRIVNPKIRFYQASTSEMFGKVQAIPQIESTPFYPRSPYGVAKLYA
HWITVNYRESYDIFGCSGILFNHESPLRGREFVTRKITDSVAKIKLGQLDVLELGNMDAKRDWGFAKEYVEGMWRMLQAD
EPDTFVLATNRTETVRDFVRMAFKAAGVDLEFKGSDEQEIAVDVATGKTLVRVNPKFHRPAEVDLLIGNPEKAKQKLGWE
PKTTLEELCAMMVEADLRRNERGFSF
>Mature_345_residues
SKKTSIITGITGQDGAYLAELLLDKGYTVYGTYRRTSSVNFWRIEELGIAKHPNLHLVEYDLTDLSASIRLLQTTGATEV
YNLAAQSFVGVSFDQPVTTAEITGVGPLNLLEAIRIVNPKIRFYQASTSEMFGKVQAIPQIESTPFYPRSPYGVAKLYAH
WITVNYRESYDIFGCSGILFNHESPLRGREFVTRKITDSVAKIKLGQLDVLELGNMDAKRDWGFAKEYVEGMWRMLQADE
PDTFVLATNRTETVRDFVRMAFKAAGVDLEFKGSDEQEIAVDVATGKTLVRVNPKFHRPAEVDLLIGNPEKAKQKLGWEP
KTTLEELCAMMVEADLRRNERGFSF

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=344, Percent_Identity=54.3604651162791, Blast_Score=375, Evalue=1e-104,
Organism=Escherichia coli, GI1788366, Length=349, Percent_Identity=52.7220630372493, Blast_Score=361, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17539422, Length=343, Percent_Identity=56.5597667638484, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17539424, Length=343, Percent_Identity=56.5597667638484, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI133901788, Length=343, Percent_Identity=56.5597667638484, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI133901786, Length=343, Percent_Identity=56.5597667638484, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI133901790, Length=343, Percent_Identity=56.5597667638484, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17507723, Length=344, Percent_Identity=56.3953488372093, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24158427, Length=346, Percent_Identity=52.3121387283237, Blast_Score=362, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 38944; Mature: 38813

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKTSIITGITGQDGAYLAELLLDKGYTVYGTYRRTSSVNFWRIEELGIAKHPNLHLVE
CCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCCCEEEEE
YDLTDLSASIRLLQTTGATEVYNLAAQSFVGVSFDQPVTTAEITGVGPLNLLEAIRIVNP
EECCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHCCC
KIRFYQASTSEMFGKVQAIPQIESTPFYPRSPYGVAKLYAHWITVNYRESYDIFGCSGIL
CEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEEEEEEEEECCCCCEEEEEEEE
FNHESPLRGREFVTRKITDSVAKIKLGQLDVLELGNMDAKRDWGFAKEYVEGMWRMLQAD
ECCCCCCCHHHHHHHHHHHHHHHEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
EPDTFVLATNRTETVRDFVRMAFKAAGVDLEFKGSDEQEIAVDVATGKTLVRVNPKFHRP
CCCEEEEECCCHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEEEECCCEEEEECCCCCCC
AEVDLLIGNPEKAKQKLGWEPKTTLEELCAMMVEADLRRNERGFSF
CEEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SKKTSIITGITGQDGAYLAELLLDKGYTVYGTYRRTSSVNFWRIEELGIAKHPNLHLVE
CCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCCCEEEEE
YDLTDLSASIRLLQTTGATEVYNLAAQSFVGVSFDQPVTTAEITGVGPLNLLEAIRIVNP
EECCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHCCC
KIRFYQASTSEMFGKVQAIPQIESTPFYPRSPYGVAKLYAHWITVNYRESYDIFGCSGIL
CEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEEEEEEEEECCCCCEEEEEEEE
FNHESPLRGREFVTRKITDSVAKIKLGQLDVLELGNMDAKRDWGFAKEYVEGMWRMLQAD
ECCCCCCCHHHHHHHHHHHHHHHEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
EPDTFVLATNRTETVRDFVRMAFKAAGVDLEFKGSDEQEIAVDVATGKTLVRVNPKFHRP
CCCEEEEECCCHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEEEECCCEEEEECCCCCCC
AEVDLLIGNPEKAKQKLGWEPKTTLEELCAMMVEADLRRNERGFSF
CEEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]