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The map label for this gene is nitA [H]

Identifier: 115350649

GI number: 115350649

Start: 666983

End: 667906

Strand: Direct

Name: nitA [H]

Synonym: Bamb_0595

Alternate gene names: 115350649

Gene position: 666983-667906 (Clockwise)

Preceding gene: 115350643

Following gene: 115350659

Centisome position: 18.75

GC content: 71.65

Gene sequence:

>924_bases
ATGTCCACCTCAGTCATCGCCGCGCTGCAGATCGGCGCCTCGCCCGCCGGCACGCGCGCCACGCTCGACACGATCCTCGG
CTACGAAACCGCGATCCGCGACAGCGGCGCGTCGCTCGTCGTGCTGCCCGAGGCCGTGCTCGGCGGCTATCCGAAAGGCG
AGAGCTTCGGCACGCGGCTCGGCTATCGGTTGCCCGAAGGCCGCGACGCGTATGCGCGCTACGCCGCGCATGCGATCGAC
GTGCCGGGGCCCGAAACCGACGAACTGGCCGCGCTGTCGCAGCGTACGGGGGCGAGCCTCGTGGTCGGCGTGATCGAGCG
AGGCGGCAGCACGCTGTACTGCACGGCGCTGTTCTTCGACCCGCGCGACGGACTCGTCGCGAAGCACCGCAAGCTGATGC
CGACCGGCACCGAGCGGCTGATCTGGGGGCAGGGCGACGGCTCGACGCTCCCGGTGGTCGACACGGCCGCGGGCCGCGCG
GGTGCCGCGATCTGCTGGGAGAACCACATGCCGCTGCTGCGCTGCGCGATGTATGCGAAAGGCGTGCAGATCTGGTGCGC
GCCGACCGTCGACGAGCGCGACCTGTGGCAAAGCTCGATGCGCCACATCGCGCACGAGGGGCGCTGCTTCGTCGTGAGCG
CGTGCCAGGTGCAGCCGTCGCCGCGCGCGCTGGGCATCGACGTGCCGGGCTGGGATCCCGAGCGGCCGCTGATCCGCGGC
GGCAGCGTGATCGTCGGGCCGCTCGGCGACCTGCTGACGGAGCCGCTGATCGGCGAGGCGGGCCTCGTCACCGCGCGCAT
CGATACCGACGAACTCGTGCGGGCGCGCTACGACTTCGACGTGGTCGGGCACTATGCGCGCGCCGACGTGTTTTCGCTGC
ACGTCGACGAGCGGCCGAAGCGGCCGGTGGTGTTCGAAGGCTGA

Upstream 100 bases:

>100_bases
CGATATCATCCGCATTCATGTCAGTTATCGTTGTATCGATTTGATCGATGAAATGATATCGATAAAGATGGCGTCACCTC
GTTCACCCGGAGACGCCTTC

Downstream 100 bases:

>100_bases
CGGGCGTTGCCGGGGCGCCGACGTCCCGGCGCTCGAAGGGCGGCGCGGCTGGCGGGGCGGCATTCGGCTGCCGCCGCGCG
CGTCAGCGCATCGGCGCTTC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSTSVIAALQIGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGESFGTRLGYRLPEGRDAYARYAAHAID
VPGPETDELAALSQRTGASLVVGVIERGGSTLYCTALFFDPRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVDTAAGRA
GAAICWENHMPLLRCAMYAKGVQIWCAPTVDERDLWQSSMRHIAHEGRCFVVSACQVQPSPRALGIDVPGWDPERPLIRG
GSVIVGPLGDLLTEPLIGEAGLVTARIDTDELVRARYDFDVVGHYARADVFSLHVDERPKRPVVFEG

Sequences:

>Translated_307_residues
MSTSVIAALQIGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGESFGTRLGYRLPEGRDAYARYAAHAID
VPGPETDELAALSQRTGASLVVGVIERGGSTLYCTALFFDPRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVDTAAGRA
GAAICWENHMPLLRCAMYAKGVQIWCAPTVDERDLWQSSMRHIAHEGRCFVVSACQVQPSPRALGIDVPGWDPERPLIRG
GSVIVGPLGDLLTEPLIGEAGLVTARIDTDELVRARYDFDVVGHYARADVFSLHVDERPKRPVVFEG
>Mature_306_residues
STSVIAALQIGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGESFGTRLGYRLPEGRDAYARYAAHAIDV
PGPETDELAALSQRTGASLVVGVIERGGSTLYCTALFFDPRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVDTAAGRAG
AAICWENHMPLLRCAMYAKGVQIWCAPTVDERDLWQSSMRHIAHEGRCFVVSACQVQPSPRALGIDVPGWDPERPLIRGG
SVIVGPLGDLLTEPLIGEAGLVTARIDTDELVRARYDFDVVGHYARADVFSLHVDERPKRPVVFEG

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17557111, Length=306, Percent_Identity=47.0588235294118, Blast_Score=281, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6322027, Length=197, Percent_Identity=65.989847715736, Blast_Score=270, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6322026, Length=111, Percent_Identity=45.945945945946, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 32904; Mature: 32773

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00921 NITRIL_CHT_2 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSVIAALQIGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGESFGTRL
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCHHHHC
GYRLPEGRDAYARYAAHAIDVPGPETDELAALSQRTGASLVVGVIERGGSTLYCTALFFD
CCCCCCCCHHHHHHHHHEECCCCCCHHHHHHHHHCCCCEEEEEEHHCCCCEEEEEEEEEC
PRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVDTAAGRAGAAICWENHMPLLRCAMYAK
CCCCHHHHHHCCCCCCCCEEEEECCCCCEECEEECCCCCCCEEEEECCCCHHHHHHHHHC
GVQIWCAPTVDERDLWQSSMRHIAHEGRCFVVSACQVQPSPRALGIDVPGWDPERPLIRG
CEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEECCCCCCCCCEEEC
GSVIVGPLGDLLTEPLIGEAGLVTARIDTDELVRARYDFDVVGHYARADVFSLHVDERPK
CCEEEECCHHHHCCCCCCCCCEEEEEECHHHHHHHHCCHHHHHHHHHCEEEEEEECCCCC
RPVVFEG
CCCEECC
>Mature Secondary Structure 
STSVIAALQIGASPAGTRATLDTILGYETAIRDSGASLVVLPEAVLGGYPKGESFGTRL
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCHHHHC
GYRLPEGRDAYARYAAHAIDVPGPETDELAALSQRTGASLVVGVIERGGSTLYCTALFFD
CCCCCCCCHHHHHHHHHEECCCCCCHHHHHHHHHCCCCEEEEEEHHCCCCEEEEEEEEEC
PRDGLVAKHRKLMPTGTERLIWGQGDGSTLPVVDTAAGRAGAAICWENHMPLLRCAMYAK
CCCCHHHHHHCCCCCCCCEEEEECCCCCEECEEECCCCCCCEEEEECCCCHHHHHHHHHC
GVQIWCAPTVDERDLWQSSMRHIAHEGRCFVVSACQVQPSPRALGIDVPGWDPERPLIRG
CEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEECCCCCCCCCEEEC
GSVIVGPLGDLLTEPLIGEAGLVTARIDTDELVRARYDFDVVGHYARADVFSLHVDERPK
CCEEEECCHHHHCCCCCCCCCEEEEEECHHHHHHHHCCHHHHHHHHHCEEEEEEECCCCC
RPVVFEG
CCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]