Click here to switch to the map view.

The map label for this gene is ampD [H]

Identifier: 115350550

GI number: 115350550

Start: 559227

End: 559817

Strand: Direct

Name: ampD [H]

Synonym: Bamb_0496

Alternate gene names: 115350550

Gene position: 559227-559817 (Clockwise)

Preceding gene: 115350549

Following gene: 115350551

Centisome position: 15.72

GC content: 69.04

Gene sequence:

>591_bases
ATGAGCGACGCAGCGCCGCTGTCGGTCGACGCACACGGCTGGGTGCGCGAAGCGCGCCATGCGCCGTCGCCGAATTTCGA
GGCGCGGCCCGCGGGCGCGGTGCCGACGCTCGTCGTCGTCCACAACATCAGCCTGCCGCCCGGCGAGTTCGGCGGCGACG
CGATCGAGTCGCTGTTCCTGAATCGCCTCGATTGCGATGCGCATCCCTATTACCAGAGCCACCTGCGCGGCGTGCGCGTG
TCCGCGCACTTCCTGATCCGCCGCGACGGCGAGCTCGTGCAGTTCGTGTCGTGCGACGAGCGCGCATGGCATGCGGGCTC
GTCGGAATTCTTCGGCCGGCCGCGCTGCAACGATTTCTCGATCGGCATCGAGCTCGAGGGCGCGGACGACGTGCCGTTCG
ACCCGGCGCAGTACGCGACGCTCGGCGTGCTCGCCCGCACGCTCGCCGCGCGCTACCCGATCGATGCGGTCGCCGGGCAT
TCCGATGTCGCGCCGGGCCGCAAGACCGACCCGGGTCCGCATTTCGATTGGCAACGCTTCGCAGGCGATGCCGGCTTTTC
GGCCGAATACTTTCCTTTCCGTCAGCACTGA

Upstream 100 bases:

>100_bases
ATGCGCCGAAGGGCGACGCCGTCGCCGCGGGCGGCGAATACTTCTGCAGCACCGACCATGCGCGGCGCCACGGCGCACGC
GCGAGCGGCCACGACGCGCG

Downstream 100 bases:

>100_bases
GCGCAGGGTTTTCGAGCCCGCGAAAAAAATTTTTCCGACCAATGCCTTGTCACGTCTGCGCCTGCGCGGGGCGGGGGTGA
TGTCAATACCTTCAGCGCAA

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MSDAAPLSVDAHGWVREARHAPSPNFEARPAGAVPTLVVVHNISLPPGEFGGDAIESLFLNRLDCDAHPYYQSHLRGVRV
SAHFLIRRDGELVQFVSCDERAWHAGSSEFFGRPRCNDFSIGIELEGADDVPFDPAQYATLGVLARTLAARYPIDAVAGH
SDVAPGRKTDPGPHFDWQRFAGDAGFSAEYFPFRQH

Sequences:

>Translated_196_residues
MSDAAPLSVDAHGWVREARHAPSPNFEARPAGAVPTLVVVHNISLPPGEFGGDAIESLFLNRLDCDAHPYYQSHLRGVRV
SAHFLIRRDGELVQFVSCDERAWHAGSSEFFGRPRCNDFSIGIELEGADDVPFDPAQYATLGVLARTLAARYPIDAVAGH
SDVAPGRKTDPGPHFDWQRFAGDAGFSAEYFPFRQH
>Mature_195_residues
SDAAPLSVDAHGWVREARHAPSPNFEARPAGAVPTLVVVHNISLPPGEFGGDAIESLFLNRLDCDAHPYYQSHLRGVRVS
AHFLIRRDGELVQFVSCDERAWHAGSSEFFGRPRCNDFSIGIELEGADDVPFDPAQYATLGVLARTLAARYPIDAVAGHS
DVAPGRKTDPGPHFDWQRFAGDAGFSAEYFPFRQH

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=171, Percent_Identity=57.3099415204678, Blast_Score=197, Evalue=4e-52,
Organism=Escherichia coli, GI1787092, Length=125, Percent_Identity=43.2, Blast_Score=84, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 21497; Mature: 21366

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAAPLSVDAHGWVREARHAPSPNFEARPAGAVPTLVVVHNISLPPGEFGGDAIESLFL
CCCCCCCEECCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHH
NRLDCDAHPYYQSHLRGVRVSAHFLIRRDGELVQFVSCDERAWHAGSSEFFGRPRCNDFS
HHCCCCCCCHHHHHHCEEEEEEEEEEECCCCEEEEECCCCHHHCCCCHHHCCCCCCCCEE
IGIELEGADDVPFDPAQYATLGVLARTLAARYPIDAVAGHSDVAPGRKTDPGPHFDWQRF
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCHHHH
AGDAGFSAEYFPFRQH
CCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDAAPLSVDAHGWVREARHAPSPNFEARPAGAVPTLVVVHNISLPPGEFGGDAIESLFL
CCCCCCEECCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHH
NRLDCDAHPYYQSHLRGVRVSAHFLIRRDGELVQFVSCDERAWHAGSSEFFGRPRCNDFS
HHCCCCCCCHHHHHHCEEEEEEEEEEECCCCEEEEECCCCHHHCCCCHHHCCCCCCCCEE
IGIELEGADDVPFDPAQYATLGVLARTLAARYPIDAVAGHSDVAPGRKTDPGPHFDWQRF
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCHHHH
AGDAGFSAEYFPFRQH
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]