Click here to switch to the map view.

The map label for this gene is mutT [H]

Identifier: 115350529

GI number: 115350529

Start: 540050

End: 540508

Strand: Direct

Name: mutT [H]

Synonym: Bamb_0475

Alternate gene names: 115350529

Gene position: 540050-540508 (Clockwise)

Preceding gene: 115350528

Following gene: 115350538

Centisome position: 15.18

GC content: 68.63

Gene sequence:

>459_bases
ATGAGCACGGATTCCGCAGTGGGCGCCGTGCGCGCGCCCGACGGCCGCAAGGTGACGGAAGTCGCGGTCGGCGTGATGGT
ACAGCCCGGCGACGCGGTCGGCCGTTGCCGCTACCTGCTTGCGCAGCGGCTGCAGGGCAAGCCGTACGAAGGCTACTGGG
AGTTCCCGGGCGGCAAGCTGGAAGCCGGCGAGAGCGTCGAGGACGCGCTGGCGCGCGAGCTGCACGAGGAACTGGGCATC
GTCGTCACCGCGAGCCACCGCTGGCATACGCTCGAGCACGACTATCCGCATGCATACGTGCGGCTCTATTTCTGCAAGGT
GACGGGCTGGACGGGCGAGCCGCACAGCAAGGAAGGCCAGGCGTTCGTGTGGCAGCAACTGCCGGTCGACGTCGCGCCGC
TGTTGCCGGCGGCGCTGCCGGTGCTGGAATTGCTCGAGAAGGAAGCGGCGTCGCAATGA

Upstream 100 bases:

>100_bases
CGCGCGGCGACGCGCTCGTGTGGGCGCTCGAGCGCGGTTCGCGCTCGGGACGCGTCGCCTGGCAGTTCGCGCGCGATTGG
TCGGGTCGCAAGGAGCAGGC

Downstream 100 bases:

>100_bases
CGCCGCGGCGGCTCGCCTGAAAAGCGGGCCGCATCCGCCTGCGGCGGTACCGCGCGCCGCTCAGCTGTCGCGGCGGTCGT
CGGTGCCGTCTTCCTCGGAC

Product: NUDIX hydrolase

Products: 8-Oxo-Dgmp; Pyrophosphate. [C]

Alternate protein names: 7,8-dihydro-8-oxoguanine-triphosphatase; 8-oxo-dGTPase; dGTP pyrophosphohydrolase [H]

Number of amino acids: Translated: 152; Mature: 151

Protein sequence:

>152_residues
MSTDSAVGAVRAPDGRKVTEVAVGVMVQPGDAVGRCRYLLAQRLQGKPYEGYWEFPGGKLEAGESVEDALARELHEELGI
VVTASHRWHTLEHDYPHAYVRLYFCKVTGWTGEPHSKEGQAFVWQQLPVDVAPLLPAALPVLELLEKEAASQ

Sequences:

>Translated_152_residues
MSTDSAVGAVRAPDGRKVTEVAVGVMVQPGDAVGRCRYLLAQRLQGKPYEGYWEFPGGKLEAGESVEDALARELHEELGI
VVTASHRWHTLEHDYPHAYVRLYFCKVTGWTGEPHSKEGQAFVWQQLPVDVAPLLPAALPVLELLEKEAASQ
>Mature_151_residues
STDSAVGAVRAPDGRKVTEVAVGVMVQPGDAVGRCRYLLAQRLQGKPYEGYWEFPGGKLEAGESVEDALARELHEELGIV
VTASHRWHTLEHDYPHAYVRLYFCKVTGWTGEPHSKEGQAFVWQQLPVDVAPLLPAALPVLELLEKEAASQ

Specific function: Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal efficiency thus lea

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1786288, Length=132, Percent_Identity=34.0909090909091, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1788056, Length=129, Percent_Identity=35.6589147286822, Blast_Score=61, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003561
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 16734; Mature: 16603

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDSAVGAVRAPDGRKVTEVAVGVMVQPGDAVGRCRYLLAQRLQGKPYEGYWEFPGGKL
CCCCCCCCEEECCCCCCHHHHEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCC
EAGESVEDALARELHEELGIVVTASHRWHTLEHDYPHAYVRLYFCKVTGWTGEPHSKEGQ
CCCCCHHHHHHHHHHHHCCEEEECCCCEEECCCCCCCEEEEEEEEEEECCCCCCCCCCCC
AFVWQQLPVDVAPLLPAALPVLELLEKEAASQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STDSAVGAVRAPDGRKVTEVAVGVMVQPGDAVGRCRYLLAQRLQGKPYEGYWEFPGGKL
CCCCCCCEEECCCCCCHHHHEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCC
EAGESVEDALARELHEELGIVVTASHRWHTLEHDYPHAYVRLYFCKVTGWTGEPHSKEGQ
CCCCCHHHHHHHHHHHHCCEEEECCCCEEECCCCCCCEEEEEEEEEEECCCCCCCCCCCC
AFVWQQLPVDVAPLLPAALPVLELLEKEAASQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 8-Oxo-Dgtp; H2O [C]

Specific reaction: 8-Oxo-Dgtp + H2O = 8-Oxo-Dgmp + Pyrophosphate. [C]

General reaction: Hydrolase; Acting on acid anhydrides; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]