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The map label for this gene is hisH

Identifier: 115350402

GI number: 115350402

Start: 391033

End: 391674

Strand: Direct

Name: hisH

Synonym: Bamb_0347

Alternate gene names: 115350402

Gene position: 391033-391674 (Clockwise)

Preceding gene: 115350401

Following gene: 115350403

Centisome position: 10.99

GC content: 66.51

Gene sequence:

>642_bases
ATGAAAACTTCGATTGCGATTGTGGATTATGGAATGGGCAACCTGCGCTCGGTCGCGCAGGCGCTCAAGAAGGCCGAACC
GGCCGCCGACGTGGCGATCGTCGACACGCCGGCCGCGATTCGCGCGGCCGACCGCGTCGTGCTGCCCGGCCAGGGCGCGA
TGCCGGACTGCATGCGCTGCCTCGGCGAATCGGGGCTGCAGGACGCGGTGATCGAGGCCTCGCGCACGAAGCCGCTGCTC
GGCGTGTGCGTCGGCGAGCAGATGCTGTTCGACTGGAGCGCGGAAGGCGACACGAAGGGCCTGGGCCTGTTGCCCGGCAA
GGTCGTGCGCTTCGAGCTCGACGGCCGGCTGCAGGACGACGGCTCGCGCTTCAAGGTGCCGCAGATGGGCTGGAACCGCG
TGCGCCAGTCGCAGCCGCACCCGCTGTGGGACGGCGTGCCCGACGACGCATACTTTTACTTCGTACACAGCTATTACGTG
AGGCCGGACAACCCGGCACATACGGTCGGCGAAACGGCATACGGCGCGCCGTTTACGTCCGCGGTCGCGCGGGACAATCT
CTTCGCGACCCAATTCCACCCCGAGAAAAGCGCGGAAGTCGGGTTGCGTCTGTATCGCAACTTCGTACACTGGAAGCCGT
GA

Upstream 100 bases:

>100_bases
CCGGCATCAACATCGCCACGCGGCTGATGGGTCTGATGCTGTCGGCGCTGGCGGTGGAATTCATCGTCAACGGACTGAGG
GCGCTACTGCCTGCACTGAG

Downstream 100 bases:

>100_bases
GCGTCGCGTGCGTGCTACAGTCGGCGGGCCGGAACGGCCTGTCGCACGATGCGCGCACACGAAAGCGCCGAAAGAGTTGT
ACTAAACTAGCGAGGCGGCG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRCLGESGLQDAVIEASRTKPLL
GVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRLQDDGSRFKVPQMGWNRVRQSQPHPLWDGVPDDAYFYFVHSYYV
RPDNPAHTVGETAYGAPFTSAVARDNLFATQFHPEKSAEVGLRLYRNFVHWKP

Sequences:

>Translated_213_residues
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRCLGESGLQDAVIEASRTKPLL
GVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRLQDDGSRFKVPQMGWNRVRQSQPHPLWDGVPDDAYFYFVHSYYV
RPDNPAHTVGETAYGAPFTSAVARDNLFATQFHPEKSAEVGLRLYRNFVHWKP
>Mature_213_residues
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRCLGESGLQDAVIEASRTKPLL
GVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRLQDDGSRFKVPQMGWNRVRQSQPHPLWDGVPDDAYFYFVHSYYV
RPDNPAHTVGETAYGAPFTSAVARDNLFATQFHPEKSAEVGLRLYRNFVHWKP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=208, Percent_Identity=37.0192307692308, Blast_Score=122, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6319725, Length=218, Percent_Identity=32.1100917431193, Blast_Score=101, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 23407; Mature: 23407

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRC
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHCCEEEECCCCCCHHHHHH
LGESGLQDAVIEASRTKPLLGVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRLQDD
HCCCCCHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCEEEEEEECCEECCC
GSRFKVPQMGWNRVRQSQPHPLWDGVPDDAYFYFVHSYYVRPDNPAHTVGETAYGAPFTS
CCEEECCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCHHCCCCCCCCCHHH
AVARDNLFATQFHPEKSAEVGLRLYRNFVHWKP
HHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRC
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHCCEEEECCCCCCHHHHHH
LGESGLQDAVIEASRTKPLLGVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRLQDD
HCCCCCHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCEEEEEEECCEECCC
GSRFKVPQMGWNRVRQSQPHPLWDGVPDDAYFYFVHSYYVRPDNPAHTVGETAYGAPFTS
CCEEECCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCHHCCCCCCCCCHHH
AVARDNLFATQFHPEKSAEVGLRLYRNFVHWKP
HHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12754231 [H]