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The map label for this gene is thiE [H]

Identifier: 115350386

GI number: 115350386

Start: 377500

End: 378624

Strand: Direct

Name: thiE [H]

Synonym: Bamb_0331

Alternate gene names: 115350386

Gene position: 377500-378624 (Clockwise)

Preceding gene: 115350385

Following gene: 115350387

Centisome position: 10.61

GC content: 72.44

Gene sequence:

>1125_bases
ATGAGCGAGCGCTTTGCCGATGCGTTCTGGCCGCCGGCCGACGAGCTGGCCGAAGCGGCCGAGCGGATTCGCGCCCGCCT
GGGCGACTGGCCGGCCGGCACGACGCCGTGGCGGCTGTGCGTCGCGGCGCCCGACGTGCCGGTCGACGGCGACGTGCTGA
TCGTGTCGGCCGGCGACCGTGCCGCGCAGGCGCGCGCGTCGGCCGCATCGCGCCCCGCGGCGCCGGATGCGGTCGCGATC
GAGTTCGACGAACACGGCGCGACGCTGCATGCCGCGGGCGTGCGCCATGCGCTGGAAGCCGCGCATCCGCTCGGCGACGA
CTGGATCGCGGCACTCGCCGCATTCCTCGATTGCGGTTTCGCGCCGATCGACGCGCTGGTGCTGGCGCTCGCGTGGCGTG
ACGGCGACGAAACGCGCGGCGCGGATGCGTGGCCGGTCGACGCCGCGCGATTTCCGCGCGTCGCCGGCCTGGCCGCCGCG
CCGGAGCCCGCATTCCCGCCGTGCCCCGCGCAACTCGGCCTGTATCCGGTCGTCCCCGACGCCGAATGGGTCGAGCGCGT
GCTCGATTGCGGCGCGCGGACCGTGCAGCTGCGCGTGAAAGGCGCGCACCCTGCCGCGTTGCGCGGCGAAATCGCGCGTG
CGGTCGCGGCCGGGCGCCGCTATCCCGATGCGCGCGTGTTCATCAACGATCACTGGCAGATCGCGGTAGAAGAGGGGGCG
TACGGCGTACATCTGGGTCAGGAAGACCTGGAGACGGCCGATCTGGCCGCGATCGCGCACGCGGGCCTACGGCTCGGGCT
GTCGAGCCACGGCTATTACGAAATGTTGCGGGCATTGCACGAGCGCCCGAGCTATCTCGCGCTCGGCCCCGTGTACGCGA
CCGCGACCAAGGCAGTTGCGGCGCCGCCGCAGGGACTCGCGCGGATCGCCCGCTATGCGCGCTTCGCGGGCGCGCGGGCG
CCGCTCGTCGCGATCGGCGGCGTGGGCCTCGACGCGTTGCCTGACGTGCTCGCGACGGGCGTCGGCAGCGTCGCGGTCGT
CAGTGCGATCACGGGCGCGATCGATTATCGGGCGGCGGTTGTTGCGTTGCAGCAATGTTTTGCCCGACAATTTGACAATC
ATTGA

Upstream 100 bases:

>100_bases
GTGCGGCCTACCTCGCCGGGCCGATGGACGCGCGCGAGACCGCGCACGCGAGCACGCCGGTCGTCGGGATGCCGTTCTGG
CACCAGGACGGGGGCGGCGC

Downstream 100 bases:

>100_bases
CCGCAGGGCCTGGAACGCCGTCACGACATCATTCCAATGCAGGCCCTATAATTCGGCGTTCTGCGTTAAAGGACCGTCCG
CTCCGTGAGCCCCACTCCTA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSERFADAFWPPADELAEAAERIRARLGDWPAGTTPWRLCVAAPDVPVDGDVLIVSAGDRAAQARASAASRPAAPDAVAI
EFDEHGATLHAAGVRHALEAAHPLGDDWIAALAAFLDCGFAPIDALVLALAWRDGDETRGADAWPVDAARFPRVAGLAAA
PEPAFPPCPAQLGLYPVVPDAEWVERVLDCGARTVQLRVKGAHPAALRGEIARAVAAGRRYPDARVFINDHWQIAVEEGA
YGVHLGQEDLETADLAAIAHAGLRLGLSSHGYYEMLRALHERPSYLALGPVYATATKAVAAPPQGLARIARYARFAGARA
PLVAIGGVGLDALPDVLATGVGSVAVVSAITGAIDYRAAVVALQQCFARQFDNH

Sequences:

>Translated_374_residues
MSERFADAFWPPADELAEAAERIRARLGDWPAGTTPWRLCVAAPDVPVDGDVLIVSAGDRAAQARASAASRPAAPDAVAI
EFDEHGATLHAAGVRHALEAAHPLGDDWIAALAAFLDCGFAPIDALVLALAWRDGDETRGADAWPVDAARFPRVAGLAAA
PEPAFPPCPAQLGLYPVVPDAEWVERVLDCGARTVQLRVKGAHPAALRGEIARAVAAGRRYPDARVFINDHWQIAVEEGA
YGVHLGQEDLETADLAAIAHAGLRLGLSSHGYYEMLRALHERPSYLALGPVYATATKAVAAPPQGLARIARYARFAGARA
PLVAIGGVGLDALPDVLATGVGSVAVVSAITGAIDYRAAVVALQQCFARQFDNH
>Mature_373_residues
SERFADAFWPPADELAEAAERIRARLGDWPAGTTPWRLCVAAPDVPVDGDVLIVSAGDRAAQARASAASRPAAPDAVAIE
FDEHGATLHAAGVRHALEAAHPLGDDWIAALAAFLDCGFAPIDALVLALAWRDGDETRGADAWPVDAARFPRVAGLAAAP
EPAFPPCPAQLGLYPVVPDAEWVERVLDCGARTVQLRVKGAHPAALRGEIARAVAAGRRYPDARVFINDHWQIAVEEGAY
GVHLGQEDLETADLAAIAHAGLRLGLSSHGYYEMLRALHERPSYLALGPVYATATKAVAAPPQGLARIARYARFAGARAP
LVAIGGVGLDALPDVLATGVGSVAVVSAITGAIDYRAAVVALQQCFARQFDNH

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=205, Percent_Identity=52.6829268292683, Blast_Score=203, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 39256; Mature: 39124

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERFADAFWPPADELAEAAERIRARLGDWPAGTTPWRLCVAAPDVPVDGDVLIVSAGDR
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCH
AAQARASAASRPAAPDAVAIEFDEHGATLHAAGVRHALEAAHPLGDDWIAALAAFLDCGF
HHHHHHHHCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
APIDALVLALAWRDGDETRGADAWPVDAARFPRVAGLAAAPEPAFPPCPAQLGLYPVVPD
CHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHHCCCCCCCCCCCCCCHHCCCEECCCC
AEWVERVLDCGARTVQLRVKGAHPAALRGEIARAVAAGRRYPDARVFINDHWQIAVEEGA
HHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCC
YGVHLGQEDLETADLAAIAHAGLRLGLSSHGYYEMLRALHERPSYLALGPVYATATKAVA
EEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHC
APPQGLARIARYARFAGARAPLVAIGGVGLDALPDVLATGVGSVAVVSAITGAIDYRAAV
CCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VALQQCFARQFDNH
HHHHHHHHHHHCCC
>Mature Secondary Structure 
SERFADAFWPPADELAEAAERIRARLGDWPAGTTPWRLCVAAPDVPVDGDVLIVSAGDR
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCH
AAQARASAASRPAAPDAVAIEFDEHGATLHAAGVRHALEAAHPLGDDWIAALAAFLDCGF
HHHHHHHHCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
APIDALVLALAWRDGDETRGADAWPVDAARFPRVAGLAAAPEPAFPPCPAQLGLYPVVPD
CHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCHHCCCCCCCCCCCCCCHHCCCEECCCC
AEWVERVLDCGARTVQLRVKGAHPAALRGEIARAVAAGRRYPDARVFINDHWQIAVEEGA
HHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCC
YGVHLGQEDLETADLAAIAHAGLRLGLSSHGYYEMLRALHERPSYLALGPVYATATKAVA
EEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHC
APPQGLARIARYARFAGARAPLVAIGGVGLDALPDVLATGVGSVAVVSAITGAIDYRAAV
CCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
VALQQCFARQFDNH
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA