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The map label for this gene is nodW [H]

Identifier: 116249453

GI number: 116249453

Start: 862329

End: 862970

Strand: Reverse

Name: nodW [H]

Synonym: pRL120792

Alternate gene names: 116249453

Gene position: 862970-862329 (Counterclockwise)

Preceding gene: 116249454

Following gene: 116249452

Centisome position: 99.19

GC content: 60.75

Gene sequence:

>642_bases
ATGACGGCCGACGACCATATCGTCTTCATCGTCGACGACGATGAACGTATCCGGGAAGCGCTCGGCGAACTGCTGGACTC
GCACGGCATGAATGCCATCGCCTTCGAATCGGCCTGCGACTACGTGAGAGCGGACAAGCCGGACGTGCCCGCCTGCCTCG
TGCTGGATATCGAGCTGCCTGACATCAACGGCCTCGATCTGCAGAGGCAGATCGCCGACAGGGATCATCCGCCGATCGTC
TTCATTACGGGACACGGCGACATTCCCTCCTCCGTGCGTGCGATCAAGCATGGCGCGGTGGACTTCCTGACCAAGCCGTT
CAGCGATGCGAATCTCATGGCGGCAATCCACGCGGCGATTGCTCAGGATCGGGAAAAGAGAGCCGAGCGCGCCGAGATCG
ACGTGCTGAGACAACACTATCTCGACCTGACGCCGCGCGAGCGCGAGGTGCTGCCGCTGGTCGTCAGCGGTCTTCTCAAC
AAGCAGGCCGCAGCCGAATTGGGGATCAGCGAAGTGACGCTGCAGATCCACAGAAGAAACGTAATGCAGAAAATGGCCGC
GGCATCGCTCGCCGATCTGGTGCGGATCGCGGAGAGATTGGAAATACCGATAACCCACTCGCGCCGAGTGGGAGGGAATT
GA

Upstream 100 bases:

>100_bases
CGATCGTCGAACTGCACGGCGGACGATTGTGGGCCGAGAAGAACAATCCTTGCGGAGCGACGTTGATCTTCACATTGCCT
ATTGAAACGAAGGCGGCGCC

Downstream 100 bases:

>100_bases
AACATGAACAAGACAAGACACGTCGTGGCAATTGTCGATGACGATCCAAGGTTGCTTGAATCGATGGGCGACCTTCTGGA
ATCTGCGGGTTATGTGGCCC

Product: putative NodW-like nodulation protein transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MTADDHIVFIVDDDERIREALGELLDSHGMNAIAFESACDYVRADKPDVPACLVLDIELPDINGLDLQRQIADRDHPPIV
FITGHGDIPSSVRAIKHGAVDFLTKPFSDANLMAAIHAAIAQDREKRAERAEIDVLRQHYLDLTPREREVLPLVVSGLLN
KQAAAELGISEVTLQIHRRNVMQKMAAASLADLVRIAERLEIPITHSRRVGGN

Sequences:

>Translated_213_residues
MTADDHIVFIVDDDERIREALGELLDSHGMNAIAFESACDYVRADKPDVPACLVLDIELPDINGLDLQRQIADRDHPPIV
FITGHGDIPSSVRAIKHGAVDFLTKPFSDANLMAAIHAAIAQDREKRAERAEIDVLRQHYLDLTPREREVLPLVVSGLLN
KQAAAELGISEVTLQIHRRNVMQKMAAASLADLVRIAERLEIPITHSRRVGGN
>Mature_212_residues
TADDHIVFIVDDDERIREALGELLDSHGMNAIAFESACDYVRADKPDVPACLVLDIELPDINGLDLQRQIADRDHPPIVF
ITGHGDIPSSVRAIKHGAVDFLTKPFSDANLMAAIHAAIAQDREKRAERAEIDVLRQHYLDLTPREREVLPLVVSGLLNK
QAAAELGISEVTLQIHRRNVMQKMAAASLADLVRIAERLEIPITHSRRVGGN

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=126, Percent_Identity=35.7142857142857, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1790102, Length=192, Percent_Identity=30.7291666666667, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1790860, Length=115, Percent_Identity=29.5652173913043, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23569; Mature: 23438

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADDHIVFIVDDDERIREALGELLDSHGMNAIAFESACDYVRADKPDVPACLVLDIELP
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCEEEEEEEECC
DINGLDLQRQIADRDHPPIVFITGHGDIPSSVRAIKHGAVDFLTKPFSDANLMAAIHAAI
CCCCCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AQDREKRAERAEIDVLRQHYLDLTPREREVLPLVVSGLLNKQAAAELGISEVTLQIHRRN
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VMQKMAAASLADLVRIAERLEIPITHSRRVGGN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TADDHIVFIVDDDERIREALGELLDSHGMNAIAFESACDYVRADKPDVPACLVLDIELP
CCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCEEEEEEEECC
DINGLDLQRQIADRDHPPIVFITGHGDIPSSVRAIKHGAVDFLTKPFSDANLMAAIHAAI
CCCCCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AQDREKRAERAEIDVLRQHYLDLTPREREVLPLVVSGLLNKQAAAELGISEVTLQIHRRN
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VMQKMAAASLADLVRIAERLEIPITHSRRVGGN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]