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The map label for this gene is galE [C]

Identifier: 116249440

GI number: 116249440

Start: 848617

End: 849450

Strand: Reverse

Name: galE [C]

Synonym: pRL120778

Alternate gene names: 116249440

Gene position: 849450-848617 (Counterclockwise)

Preceding gene: 116249441

Following gene: 116249439

Centisome position: 97.64

GC content: 60.55

Gene sequence:

>834_bases
ATGATGAAACGGGTCGCTGTGACGGGTGCTGCCGGACGTGTAGGGACGCTGCTGCGCCCGCTTCTTCGGTCGCATTTCGA
GCACCTCAGCCTGATCGATCTCCAGGAGCCCGACGAGCTTGGCGAAAACGAGAATTTCGTCAGGGCCGACTTTACAAAGC
TCGACGAGGCGACGGCTGCGCTGAAGGATATCGATGGCGTCATCCATCTGGCGGGTATTGCAAGCGGCACGGACATGAAC
GCCATTCTGCACGCGAACGTGCTTGGAACATACAATCTCTACGAAGCGGCGCGGATCAACAAGGTCCAGCGGGTCGTCTA
TGCATCGAGCAATCATGCCACGGGCTTTTATCCGCGCGGCCAGCTCGTGTCGCCGCTCGACCCGATGCGCCCCGACAGCC
CCTATGGACTTTCCAAATGCTGGGGCGAGCTTGTGGCCGGACTTTACTACGACACGTCGGGCATTCGCTCTCTTTCCATC
CGCATCGGCAATGCGGGCACCTATCCTAACAGCGAACGATCGATTGCGATCTGGATCAGCGCGCGAGACCTGGCGCAATT
GGTCAGGATCGGGCTTTCGCACCCGTTGATCGCAGCAACAGTGGTCTACGGCGTTTCTGACACCGAGGAGAAGTGGTGGG
AGAACGATCTGGCGACACGATTGGGCTACCAGCCACAGGACCGGCCGCGTGACCACGCCCTGATCGAAGAGGGATCCGAA
GGGCCAGTCGCGCTGGCGTTCCAGGGTGGCGGGTTCTGTGAGATCAATCACGACGGCACCATCCGCATGCGCGACGCCGA
TGGCTTGGCCAAGAGCCCGGAGACGGTTGAATGA

Upstream 100 bases:

>100_bases
CGCGCACCGGGAAACCGAAGCGCATGATGCGATGAAAAGACACATACTGAATGCTCGCGCCCGCATGTTCCAGGGCGTTT
GAGCGATAATGGGAGGAGAG

Downstream 100 bases:

>100_bases
CGGCGCCTCGGATCATCCGCCCGGACGTCCGTTCCGTTATCCAGCAACCGCTGACGGTCGGTGAATCGCCGGTATGGGAC
GAGGAGACCGGTACTCTGTG

Product: putative dehydratase/epimerase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MMKRVAVTGAAGRVGTLLRPLLRSHFEHLSLIDLQEPDELGENENFVRADFTKLDEATAALKDIDGVIHLAGIASGTDMN
AILHANVLGTYNLYEAARINKVQRVVYASSNHATGFYPRGQLVSPLDPMRPDSPYGLSKCWGELVAGLYYDTSGIRSLSI
RIGNAGTYPNSERSIAIWISARDLAQLVRIGLSHPLIAATVVYGVSDTEEKWWENDLATRLGYQPQDRPRDHALIEEGSE
GPVALAFQGGGFCEINHDGTIRMRDADGLAKSPETVE

Sequences:

>Translated_277_residues
MMKRVAVTGAAGRVGTLLRPLLRSHFEHLSLIDLQEPDELGENENFVRADFTKLDEATAALKDIDGVIHLAGIASGTDMN
AILHANVLGTYNLYEAARINKVQRVVYASSNHATGFYPRGQLVSPLDPMRPDSPYGLSKCWGELVAGLYYDTSGIRSLSI
RIGNAGTYPNSERSIAIWISARDLAQLVRIGLSHPLIAATVVYGVSDTEEKWWENDLATRLGYQPQDRPRDHALIEEGSE
GPVALAFQGGGFCEINHDGTIRMRDADGLAKSPETVE
>Mature_277_residues
MMKRVAVTGAAGRVGTLLRPLLRSHFEHLSLIDLQEPDELGENENFVRADFTKLDEATAALKDIDGVIHLAGIASGTDMN
AILHANVLGTYNLYEAARINKVQRVVYASSNHATGFYPRGQLVSPLDPMRPDSPYGLSKCWGELVAGLYYDTSGIRSLSI
RIGNAGTYPNSERSIAIWISARDLAQLVRIGLSHPLIAATVVYGVSDTEEKWWENDLATRLGYQPQDRPRDHALIEEGSE
GPVALAFQGGGFCEINHDGTIRMRDADGLAKSPETVE

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 30259; Mature: 30259

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKRVAVTGAAGRVGTLLRPLLRSHFEHLSLIDLQEPDELGENENFVRADFTKLDEATAA
CCCEEEEECCCCHHHHHHHHHHHHHHHHEEEECCCCCHHCCCCCCEEEHHHHHHHHHHHH
LKDIDGVIHLAGIASGTDMNAILHANVLGTYNLYEAARINKVQRVVYASSNHATGFYPRG
HHHHCCEEEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHEEEECCCCCCCCCCCC
QLVSPLDPMRPDSPYGLSKCWGELVAGLYYDTSGIRSLSIRIGNAGTYPNSERSIAIWIS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEEEEEE
ARDLAQLVRIGLSHPLIAATVVYGVSDTEEKWWENDLATRLGYQPQDRPRDHALIEEGSE
HHHHHHHHHHCCCCCHHHHHEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCC
GPVALAFQGGGFCEINHDGTIRMRDADGLAKSPETVE
CCEEEEECCCCEEEECCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MMKRVAVTGAAGRVGTLLRPLLRSHFEHLSLIDLQEPDELGENENFVRADFTKLDEATAA
CCCEEEEECCCCHHHHHHHHHHHHHHHHEEEECCCCCHHCCCCCCEEEHHHHHHHHHHHH
LKDIDGVIHLAGIASGTDMNAILHANVLGTYNLYEAARINKVQRVVYASSNHATGFYPRG
HHHHCCEEEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHEEEECCCCCCCCCCCC
QLVSPLDPMRPDSPYGLSKCWGELVAGLYYDTSGIRSLSIRIGNAGTYPNSERSIAIWIS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEEEEEE
ARDLAQLVRIGLSHPLIAATVVYGVSDTEEKWWENDLATRLGYQPQDRPRDHALIEEGSE
HHHHHHHHHHCCCCCHHHHHEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCC
GPVALAFQGGGFCEINHDGTIRMRDADGLAKSPETVE
CCEEEEECCCCEEEECCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]