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The map label for this gene is glgB

Identifier: 116249372

GI number: 116249372

Start: 772698

End: 774908

Strand: Direct

Name: glgB

Synonym: pRL120710

Alternate gene names: 116249372

Gene position: 772698-774908 (Clockwise)

Preceding gene: 116249371

Following gene: 116249380

Centisome position: 88.81

GC content: 62.64

Gene sequence:

>2211_bases
ATGAATGTTGAGCGCTCGGAACTTCTGGCGGGCATCGGACAGGATGCGCTATGGGCCTTGATCGAGGGGCGCCATGGCGA
TCCCTTTTCGATCCTCGGCCCCCACCAAAGCGGCGGCATGACGATCGTGCGGGTCTACCTGCCCGGTGCGGAAGCGGTCG
ATCTCATCGATGCGACGAGCGGCCGGGTGGTTGCACCGTTCAGCATCGCCCACCCGTCCGGCTTGTTTGCGGCGACGGTC
GCCTCGAGGACGGGATACCGGCTGCGGATCACATGGCCGGATGCAGTTCAGATAACCGAGGACCCCTACAGTTTCGGTCT
GCTGCTCGGAGAGCTCGACCTTCATCTGATATCGGAGGGCACCCACTACAGTCTCAGCCGGACGCTCGGCGCGGTGGCTA
TGTCGATCGACGGGATATCTGGCGTTCGTTTCGCCGTCTGGGCTCCGAATGCGCGCCGCGTCTCGGTTGTCGGCGACTTC
AACGCCTGGGATGGGCGGCGAAACCCGATGCGGCTGAGACCGTCGGCGGGCGTATGGGAGCTGTTCATCCCCCGGCTGGC
ACCCGGCGAAAGATACAAGTTCGAGATCGTCGATGCTGAGGGGACCTGCCTTCCGCAGAAGGCCGATCCGGTGGCGAGGG
CGAGCGAGGCCGCCCCATCCACCGCCTCTATCGTCGCATCGTCGACGCCGTTTCGATGGACCGATGACGGCTGGATGAAG
GGCCGGTCCCGGCAAGACAGGCTGGAGGGCGCGTTCTCCGTCTATGAGGTGCACGTCGGCTCCTGGCTTCGCGATCAAAA
GGACGGCAACAGGTCGCTCGACTGGGTCGAACTCAGCCAGCGGCTGGTTCCCTATGTCAGCGACATGGGCTTCACCCATA
TCGAGCTGCTTCCGATCATGGAGCATCCGTTCGGCGGCTCCTGGGGCTATCAGCCGCTCGGTCTGTTCGCTCCCACCGGC
CGATACGGGACGCCTGAGGATTTCGCTTATTTCGTCGACCGGTGCCATGGCGCCGGGCTCGGCGTCATCCTCGACTGGGT
GCCGGCCCATTTCCCCACAGATGTCTGGGGGCTTGCCCGCTTCGACGGCAGCGCGCTCTACGAGCACGAAGATCCGCGCG
AAGGCTTTCACCGCGACTGGAACACGCTGATCTACAATCTCGGCCGCAACGAGGTGAAGGGCTTCCTGATCGCCAGCGCG
CTGGAATGGCTGGAGCGCTACCATATCGACGGTTTGCGGGTCGACGCCGTTGCCTCGATGCTCTACCGCGACTACAGCCG
CAACGAGGGCGAATGGATTCCCAACCAATATGGCGGTCGCGAGAATCTGGAAGCGGTCGAATTCTTCAAGCACCTGAACA
GCATCATTCACGAGCGCTGCCCGCACGCGATGACGATCGCCGAGGAATCGACGGCCTGGCCCGGGGTGACGAAGCCGCCG
GAGCAAGGGGGGCTGGGTTTTGACATCAAATGGAACATGGGCTGGATGCATGACAGCCTGAGCTACATCGAGAAGGATCC
GATTTACCGGAGTTACGCCCACGGCACGATGACCTTCGGCATGATCTATGCCTATTCCGAACGCTTCATTCTGCCGATTT
CACATGACGAGGTCGTCTACGGAAAGGGCTCGCTGCTGACGAAGATGCCGGGCGACGAATGGCAGAAATTCGCCAATCTG
CGCAGCTACCTCGCCTTCATGTGGGGCCATCCCGGCAAGAAGCTTTTGTTCATGGGGAGCGAAATCGCTCAGCCGAGCGA
GTGGAATCACGACGGGTCGGTGACCTGGGATGTGCTGGACCAGCCTCAGCATGTGGGGATCCAGCGGCTGGTGAAGGATC
TGAACGGCCTTTACGGGGACGAGCCGGCATTGCAGTTCGGCGACTTTCATTCCGAGGGTTTTGAATGGGCGGCAGCCGAC
GACGCCGTCAATTCCGTCCTCGGCATGCTCCGTTATGCGCCCGACCGCGCTTCGTCGGTGCTGGTCATGTCAAATTTCAC
ACCGGTGCCGCGTTACGGCTACAGGATCGGTGTGCCCAGTGACGGTGTGTGGATCGAGCGGATAACGACGGATGCGCGGG
AATATGGAGGCTCGGGCCTGGTCAACGGCGCGGTGTCGAGCGAACCCGTGCCCGCCCATGGCAGGCCCGTCTCCCTGTCC
CTGACGCTGCCGCCGCTATCGACGATTTTTCTGCAGGGGCCGTCGCCCTGA

Upstream 100 bases:

>100_bases
CCTACGAGATCGCCTACGAAGCTCGCAACAGGCCGAAATGGCTTCCGATCCCGCTATCCGGCCTTACCGAAATCGTATCG
CGTCTGGCGGGGGTCACAGC

Downstream 100 bases:

>100_bases
CCGCGCGGCCGCGGAACGGTCATCTCGGTCACGGTTCCATCGTCACCTGAGACGACCATAGACAGTCATCTCGGTGGCGG
TTTCACCGTCACCTGAGATA

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2; Glycogen-branching enzyme 2; BE 2

Number of amino acids: Translated: 736; Mature: 736

Protein sequence:

>736_residues
MNVERSELLAGIGQDALWALIEGRHGDPFSILGPHQSGGMTIVRVYLPGAEAVDLIDATSGRVVAPFSIAHPSGLFAATV
ASRTGYRLRITWPDAVQITEDPYSFGLLLGELDLHLISEGTHYSLSRTLGAVAMSIDGISGVRFAVWAPNARRVSVVGDF
NAWDGRRNPMRLRPSAGVWELFIPRLAPGERYKFEIVDAEGTCLPQKADPVARASEAAPSTASIVASSTPFRWTDDGWMK
GRSRQDRLEGAFSVYEVHVGSWLRDQKDGNRSLDWVELSQRLVPYVSDMGFTHIELLPIMEHPFGGSWGYQPLGLFAPTG
RYGTPEDFAYFVDRCHGAGLGVILDWVPAHFPTDVWGLARFDGSALYEHEDPREGFHRDWNTLIYNLGRNEVKGFLIASA
LEWLERYHIDGLRVDAVASMLYRDYSRNEGEWIPNQYGGRENLEAVEFFKHLNSIIHERCPHAMTIAEESTAWPGVTKPP
EQGGLGFDIKWNMGWMHDSLSYIEKDPIYRSYAHGTMTFGMIYAYSERFILPISHDEVVYGKGSLLTKMPGDEWQKFANL
RSYLAFMWGHPGKKLLFMGSEIAQPSEWNHDGSVTWDVLDQPQHVGIQRLVKDLNGLYGDEPALQFGDFHSEGFEWAAAD
DAVNSVLGMLRYAPDRASSVLVMSNFTPVPRYGYRIGVPSDGVWIERITTDAREYGGSGLVNGAVSSEPVPAHGRPVSLS
LTLPPLSTIFLQGPSP

Sequences:

>Translated_736_residues
MNVERSELLAGIGQDALWALIEGRHGDPFSILGPHQSGGMTIVRVYLPGAEAVDLIDATSGRVVAPFSIAHPSGLFAATV
ASRTGYRLRITWPDAVQITEDPYSFGLLLGELDLHLISEGTHYSLSRTLGAVAMSIDGISGVRFAVWAPNARRVSVVGDF
NAWDGRRNPMRLRPSAGVWELFIPRLAPGERYKFEIVDAEGTCLPQKADPVARASEAAPSTASIVASSTPFRWTDDGWMK
GRSRQDRLEGAFSVYEVHVGSWLRDQKDGNRSLDWVELSQRLVPYVSDMGFTHIELLPIMEHPFGGSWGYQPLGLFAPTG
RYGTPEDFAYFVDRCHGAGLGVILDWVPAHFPTDVWGLARFDGSALYEHEDPREGFHRDWNTLIYNLGRNEVKGFLIASA
LEWLERYHIDGLRVDAVASMLYRDYSRNEGEWIPNQYGGRENLEAVEFFKHLNSIIHERCPHAMTIAEESTAWPGVTKPP
EQGGLGFDIKWNMGWMHDSLSYIEKDPIYRSYAHGTMTFGMIYAYSERFILPISHDEVVYGKGSLLTKMPGDEWQKFANL
RSYLAFMWGHPGKKLLFMGSEIAQPSEWNHDGSVTWDVLDQPQHVGIQRLVKDLNGLYGDEPALQFGDFHSEGFEWAAAD
DAVNSVLGMLRYAPDRASSVLVMSNFTPVPRYGYRIGVPSDGVWIERITTDAREYGGSGLVNGAVSSEPVPAHGRPVSLS
LTLPPLSTIFLQGPSP
>Mature_736_residues
MNVERSELLAGIGQDALWALIEGRHGDPFSILGPHQSGGMTIVRVYLPGAEAVDLIDATSGRVVAPFSIAHPSGLFAATV
ASRTGYRLRITWPDAVQITEDPYSFGLLLGELDLHLISEGTHYSLSRTLGAVAMSIDGISGVRFAVWAPNARRVSVVGDF
NAWDGRRNPMRLRPSAGVWELFIPRLAPGERYKFEIVDAEGTCLPQKADPVARASEAAPSTASIVASSTPFRWTDDGWMK
GRSRQDRLEGAFSVYEVHVGSWLRDQKDGNRSLDWVELSQRLVPYVSDMGFTHIELLPIMEHPFGGSWGYQPLGLFAPTG
RYGTPEDFAYFVDRCHGAGLGVILDWVPAHFPTDVWGLARFDGSALYEHEDPREGFHRDWNTLIYNLGRNEVKGFLIASA
LEWLERYHIDGLRVDAVASMLYRDYSRNEGEWIPNQYGGRENLEAVEFFKHLNSIIHERCPHAMTIAEESTAWPGVTKPP
EQGGLGFDIKWNMGWMHDSLSYIEKDPIYRSYAHGTMTFGMIYAYSERFILPISHDEVVYGKGSLLTKMPGDEWQKFANL
RSYLAFMWGHPGKKLLFMGSEIAQPSEWNHDGSVTWDVLDQPQHVGIQRLVKDLNGLYGDEPALQFGDFHSEGFEWAAAD
DAVNSVLGMLRYAPDRASSVLVMSNFTPVPRYGYRIGVPSDGVWIERITTDAREYGGSGLVNGAVSSEPVPAHGRPVSLS
LTLPPLSTIFLQGPSP

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=658, Percent_Identity=27.6595744680851, Blast_Score=181, Evalue=2e-45,
Organism=Escherichia coli, GI1789839, Length=726, Percent_Identity=53.7190082644628, Blast_Score=778, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=379, Percent_Identity=28.2321899736148, Blast_Score=167, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI32564391, Length=249, Percent_Identity=32.9317269076305, Blast_Score=149, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6320826, Length=617, Percent_Identity=25.1215559157212, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI28573410, Length=643, Percent_Identity=25.5054432348367, Blast_Score=169, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB2_RHIL3 (Q1M3A7)

Other databases:

- EMBL:   AM236086
- RefSeq:   YP_765213.1
- ProteinModelPortal:   Q1M3A7
- SMR:   Q1M3A7
- STRING:   Q1M3A7
- GeneID:   4398207
- GenomeReviews:   AM236086_GR
- KEGG:   rle:pRL120710
- NMPDR:   fig|216596.1.peg.6407
- eggNOG:   COG0296
- HOGENOM:   HBG287139
- OMA:   RVYHQNG
- PhylomeDB:   Q1M3A7
- ProtClustDB:   PRK05402
- BioCyc:   RLEG216596:PRL120710-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 81854; Mature: 81854

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: ACT_SITE 310-310 ACT_SITE 345-345 ACT_SITE 350-350 ACT_SITE 413-413 ACT_SITE 415-415 ACT_SITE 468-468 ACT_SITE 535-535 ACT_SITE 536-536

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVERSELLAGIGQDALWALIEGRHGDPFSILGPHQSGGMTIVRVYLPGAEAVDLIDATS
CCCCHHHHHHHCCCHHEEHEEECCCCCCCEEECCCCCCCEEEEEEEECCCCEEEEEECCC
GRVVAPFSIAHPSGLFAATVASRTGYRLRITWPDAVQITEDPYSFGLLLGELDLHLISEG
CCEEEEEEECCCCCCEEEEHHCCCCCEEEEECCCCEEEECCCHHEEEEEEEEEEEEECCC
THYSLSRTLGAVAMSIDGISGVRFAVWAPNARRVSVVGDFNAWDGRRNPMRLRPSAGVWE
CCEEHHHHHHHHHEEECCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCEEECCCCCCHH
LFIPRLAPGERYKFEIVDAEGTCLPQKADPVARASEAAPSTASIVASSTPFRWTDDGWMK
HHCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCEEEECCCCCEECCCHHHC
GRSRQDRLEGAFSVYEVHVGSWLRDQKDGNRSLDWVELSQRLVPYVSDMGFTHIELLPIM
CCCHHHHHHCCEEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEC
EHPFGGSWGYQPLGLFAPTGRYGTPEDFAYFVDRCHGAGLGVILDWVPAHFPTDVWGLAR
CCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCEEHEECCCCCCCCCHHHEEE
FDGSALYEHEDPREGFHRDWNTLIYNLGRNEVKGFLIASALEWLERYHIDGLRVDAVASM
CCCCEECCCCCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHH
LYRDYSRNEGEWIPNQYGGRENLEAVEFFKHLNSIIHERCPHAMTIAEESTAWPGVTKPP
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCC
EQGGLGFDIKWNMGWMHDSLSYIEKDPIYRSYAHGTMTFGMIYAYSERFILPISHDEVVY
CCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHCCCCHHEEEEEEECCCEEEEECCCCEEE
GKGSLLTKMPGDEWQKFANLRSYLAFMWGHPGKKLLFMGSEIAQPSEWNHDGSVTWDVLD
CCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEECC
QPQHVGIQRLVKDLNGLYGDEPALQFGDFHSEGFEWAAADDAVNSVLGMLRYAPDRASSV
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCEE
LVMSNFTPVPRYGYRIGVPSDGVWIERITTDAREYGGSGLVNGAVSSEPVPAHGRPVSLS
EEEECCCCCCCCCEEECCCCCCEEEEEEHHHHHHHCCCCEECCCCCCCCCCCCCCEEEEE
LTLPPLSTIFLQGPSP
EECCCCEEEEEECCCC
>Mature Secondary Structure
MNVERSELLAGIGQDALWALIEGRHGDPFSILGPHQSGGMTIVRVYLPGAEAVDLIDATS
CCCCHHHHHHHCCCHHEEHEEECCCCCCCEEECCCCCCCEEEEEEEECCCCEEEEEECCC
GRVVAPFSIAHPSGLFAATVASRTGYRLRITWPDAVQITEDPYSFGLLLGELDLHLISEG
CCEEEEEEECCCCCCEEEEHHCCCCCEEEEECCCCEEEECCCHHEEEEEEEEEEEEECCC
THYSLSRTLGAVAMSIDGISGVRFAVWAPNARRVSVVGDFNAWDGRRNPMRLRPSAGVWE
CCEEHHHHHHHHHEEECCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCEEECCCCCCHH
LFIPRLAPGERYKFEIVDAEGTCLPQKADPVARASEAAPSTASIVASSTPFRWTDDGWMK
HHCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCEEEECCCCCEECCCHHHC
GRSRQDRLEGAFSVYEVHVGSWLRDQKDGNRSLDWVELSQRLVPYVSDMGFTHIELLPIM
CCCHHHHHHCCEEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEC
EHPFGGSWGYQPLGLFAPTGRYGTPEDFAYFVDRCHGAGLGVILDWVPAHFPTDVWGLAR
CCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCEEHEECCCCCCCCCHHHEEE
FDGSALYEHEDPREGFHRDWNTLIYNLGRNEVKGFLIASALEWLERYHIDGLRVDAVASM
CCCCEECCCCCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHH
LYRDYSRNEGEWIPNQYGGRENLEAVEFFKHLNSIIHERCPHAMTIAEESTAWPGVTKPP
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCC
EQGGLGFDIKWNMGWMHDSLSYIEKDPIYRSYAHGTMTFGMIYAYSERFILPISHDEVVY
CCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHCCCCHHEEEEEEECCCEEEEECCCCEEE
GKGSLLTKMPGDEWQKFANLRSYLAFMWGHPGKKLLFMGSEIAQPSEWNHDGSVTWDVLD
CCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEECC
QPQHVGIQRLVKDLNGLYGDEPALQFGDFHSEGFEWAAADDAVNSVLGMLRYAPDRASSV
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCEE
LVMSNFTPVPRYGYRIGVPSDGVWIERITTDAREYGGSGLVNGAVSSEPVPAHGRPVSLS
EEEECCCCCCCCCEEECCCCCCEEEEEEHHHHHHHCCCCEECCCCCCCCCCCCCCEEEEE
LTLPPLSTIFLQGPSP
EECCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA