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The map label for this gene is purR [H]

Identifier: 116249340

GI number: 116249340

Start: 727326

End: 728333

Strand: Reverse

Name: purR [H]

Synonym: pRL120677

Alternate gene names: 116249340

Gene position: 728333-727326 (Counterclockwise)

Preceding gene: 116249347

Following gene: 116249337

Centisome position: 83.71

GC content: 64.48

Gene sequence:

>1008_bases
ATGACCACGATACGAGACGTAGCGCGCCTTGCGGGGGTTTCCATCTCGACCGTCTCGCTGGCACTCAACAGCCCGAAGCG
GGTCGGCGTCGAGACGCTTGACCGCATCCAGCAGGCGATAAAATCGACCGGTTACCGCATCGACCCGGTGGCCCAGACGC
TGGCGCGCGGACGCAGCTCGTTGATCGGTTTCGTTTCGGCCAATCTCGGCAACATGTTCTTCGGCGACATCCGTCGAGAG
ATCGAGCACCAGGCGCTCGACCACGGCTATTTCGTCCTGATCGCCGACTCCTCCGGCAGGGCCGATCTCGAACGGGCGCT
GCTGGAGCGGCTGGAAGCGCAGAAGATCGCCGGCATTGCGCTGGCGGCAAACGGGCACGGCGAGGAGTACGCCACGTTCC
TGCGCGACTTCAAAACCCCGATCGTGATGTTCGACCAGAAGGTGGAGGGGGCGGAACGCGATTTTGTCGGTTCCGACAAC
CCGCTGACCACCACCATCCTGACCGAACATCTGCTGCAGCTCGGCCACAGGCGGATCGGCTTCATTTCCGGGCCGAGCGG
CCTGCACACTGCCGACGAGCGCCTGAAGGGCTTCATGAACACGATGGCCGGCGCCGGTGTGGACATCGATCCTTCGCTGG
TGGTCGAAGGCGGCTACACCAGGACCGGCGGCCATGCACAGGCCATGCGCCTCTTGACCCGTCGCGACCGGCCGACCGCC
ATCATCGGCGCCAACAACATGACCGGCCTCGCGGCACTGCAGGTGATGCAGGAGATGGGCTTCCGCTGCCCTGACGACGT
ATCGCTGGCGATGGTCGACGACGTGCCCTGGAGCAACGTCATCACACCCCGCATCACGATGGTGGTGCAGGATGCGCAGA
AGCTCGGCGAATTGGCCGCACAACGTCTGCTGGCAAGAATAGCAAGCCCCGAGGGGGCCGCAGAGCCGCCGCAGGATTTT
ATCCTGACGCCGAGGTTCGTGCGCGGAGAGTCGACCAGGCGGTTGTGA

Upstream 100 bases:

>100_bases
TCACCAAGTTAAACCCAGTTAAACAACGGATTTGAAGCATAAATGTTGCGCCAACCAATAAAATCGGACATAACAAGGCA
TTAGAACCGGTTCGACAGAC

Downstream 100 bases:

>100_bases
GCGCTTCTATCGGTCCTCACGCGTATGCCAAAGCCGCAACACATAGATCGTCGACTGCTGGATTTCATAGCGCAGCTCGT
AACGGCCGACGAGAATGCGG

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MTTIRDVARLAGVSISTVSLALNSPKRVGVETLDRIQQAIKSTGYRIDPVAQTLARGRSSLIGFVSANLGNMFFGDIRRE
IEHQALDHGYFVLIADSSGRADLERALLERLEAQKIAGIALAANGHGEEYATFLRDFKTPIVMFDQKVEGAERDFVGSDN
PLTTTILTEHLLQLGHRRIGFISGPSGLHTADERLKGFMNTMAGAGVDIDPSLVVEGGYTRTGGHAQAMRLLTRRDRPTA
IIGANNMTGLAALQVMQEMGFRCPDDVSLAMVDDVPWSNVITPRITMVVQDAQKLGELAAQRLLARIASPEGAAEPPQDF
ILTPRFVRGESTRRL

Sequences:

>Translated_335_residues
MTTIRDVARLAGVSISTVSLALNSPKRVGVETLDRIQQAIKSTGYRIDPVAQTLARGRSSLIGFVSANLGNMFFGDIRRE
IEHQALDHGYFVLIADSSGRADLERALLERLEAQKIAGIALAANGHGEEYATFLRDFKTPIVMFDQKVEGAERDFVGSDN
PLTTTILTEHLLQLGHRRIGFISGPSGLHTADERLKGFMNTMAGAGVDIDPSLVVEGGYTRTGGHAQAMRLLTRRDRPTA
IIGANNMTGLAALQVMQEMGFRCPDDVSLAMVDDVPWSNVITPRITMVVQDAQKLGELAAQRLLARIASPEGAAEPPQDF
ILTPRFVRGESTRRL
>Mature_334_residues
TTIRDVARLAGVSISTVSLALNSPKRVGVETLDRIQQAIKSTGYRIDPVAQTLARGRSSLIGFVSANLGNMFFGDIRREI
EHQALDHGYFVLIADSSGRADLERALLERLEAQKIAGIALAANGHGEEYATFLRDFKTPIVMFDQKVEGAERDFVGSDNP
LTTTILTEHLLQLGHRRIGFISGPSGLHTADERLKGFMNTMAGAGVDIDPSLVVEGGYTRTGGHAQAMRLLTRRDRPTAI
IGANNMTGLAALQVMQEMGFRCPDDVSLAMVDDVPWSNVITPRITMVVQDAQKLGELAAQRLLARIASPEGAAEPPQDFI
LTPRFVRGESTRRL

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=335, Percent_Identity=30.1492537313433, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1790194, Length=311, Percent_Identity=28.6173633440514, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1790369, Length=305, Percent_Identity=28.1967213114754, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1789202, Length=288, Percent_Identity=29.1666666666667, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1788474, Length=332, Percent_Identity=29.8192771084337, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=25.3125, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789068, Length=323, Percent_Identity=25.6965944272446, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786540, Length=314, Percent_Identity=28.343949044586, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI48994940, Length=313, Percent_Identity=26.517571884984, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787906, Length=331, Percent_Identity=25.6797583081571, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1790715, Length=309, Percent_Identity=25.5663430420712, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789456, Length=305, Percent_Identity=24.9180327868852, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786268, Length=315, Percent_Identity=24.1269841269841, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790689, Length=325, Percent_Identity=25.2307692307692, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36448; Mature: 36317

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIRDVARLAGVSISTVSLALNSPKRVGVETLDRIQQAIKSTGYRIDPVAQTLARGRSS
CCCHHHHHHHHCCCEEEEEEEECCCHHCCHHHHHHHHHHHHHCCCEECHHHHHHHHHHHH
LIGFVSANLGNMFFGDIRREIEHQALDHGYFVLIADSSGRADLERALLERLEAQKIAGIA
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEE
LAANGHGEEYATFLRDFKTPIVMFDQKVEGAERDFVGSDNPLTTTILTEHLLQLGHRRIG
EEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEE
FISGPSGLHTADERLKGFMNTMAGAGVDIDPSLVVEGGYTRTGGHAQAMRLLTRRDRPTA
EECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCE
IIGANNMTGLAALQVMQEMGFRCPDDVSLAMVDDVPWSNVITPRITMVVQDAQKLGELAA
EEECCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
QRLLARIASPEGAAEPPQDFILTPRFVRGESTRRL
HHHHHHHCCCCCCCCCCHHCCCCCCEECCCCCCCC
>Mature Secondary Structure 
TTIRDVARLAGVSISTVSLALNSPKRVGVETLDRIQQAIKSTGYRIDPVAQTLARGRSS
CCHHHHHHHHCCCEEEEEEEECCCHHCCHHHHHHHHHHHHHCCCEECHHHHHHHHHHHH
LIGFVSANLGNMFFGDIRREIEHQALDHGYFVLIADSSGRADLERALLERLEAQKIAGIA
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEE
LAANGHGEEYATFLRDFKTPIVMFDQKVEGAERDFVGSDNPLTTTILTEHLLQLGHRRIG
EEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEE
FISGPSGLHTADERLKGFMNTMAGAGVDIDPSLVVEGGYTRTGGHAQAMRLLTRRDRPTA
EECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCE
IIGANNMTGLAALQVMQEMGFRCPDDVSLAMVDDVPWSNVITPRITMVVQDAQKLGELAA
EEECCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
QRLLARIASPEGAAEPPQDFILTPRFVRGESTRRL
HHHHHHHCCCCCCCCCCHHCCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA