Click here to switch to the map view.

The map label for this gene is ycsA [H]

Identifier: 116249294

GI number: 116249294

Start: 685852

End: 686895

Strand: Reverse

Name: ycsA [H]

Synonym: pRL120629

Alternate gene names: 116249294

Gene position: 686895-685852 (Counterclockwise)

Preceding gene: 116249295

Following gene: 116249293

Centisome position: 78.95

GC content: 62.45

Gene sequence:

>1044_bases
ATGAAAACCTACAAAATCGCCCTTCTGCCCGGAGACGGCATCGGCCGCGACGTGACGGCAGCCGCCTGGGCCGTACTCGA
AAAGGCAGCTCGCCTGAACGGATTTTCTCTTGACGCAACCAGCTATCCCTGGTCCTGCGATTACTATCTGGAGAACGGCA
GCATGATGCCCGCCGACGGCATCGAAATACTCAGATCCTTCGACGCCATCCTGCTCGGCGCCGTCGGCTGGCCTCGCAAA
GTGCCGGATTCCGTGTCGCTGCATGGGCTTCTGCTGCCGATCCGCAAGGCTTTCGTGCAATATGCCAATATCCGCCCGCA
CCGGCTGCTGCCGGGTGTGCAAGGGCCGTTGCGGTCCGATGGCTTCAACATCCTCTGCATCCGTGAAAATACCGAGGGCG
AATATTCCGGCGCCGGTGGCCGCGTCCACCAGGGCACCGATAATGAGGTGGCCGTCGAGACCTCGATCTTCACCCGCAAG
GGGGTCGAGCGCATCCTGCGTTTCGGCTTCGAGCAGGCGCGTGCGCGACGCGGCAAGCTTGCCTCGGTGACGAAGTCCAA
CGCCCAGAAATATTCGATGGTCTTCTGGGACGAAATCACCCAGAGGCTTTCTGCGGAATATCCAGATGTCGAGGTGACCA
GCTACCATATCGACGCCATGGCCGCCCGCATGGTCATGGCGCCTGATAGTCTCGACGTCGTGGTCGCCTCCAACCTGTTC
GGCGACATCCTGACCGACCTCGGCGCCGCCATCCAGGGCGGGCTCGGCTTTGCCGCCTCCGCCAATATCAATCCCGATCG
CTCGGCGCCATCGATGTTCGAACCGGTCCACGGCTCGGCGCCCGATATCGCTGATCTCGGCATCGCCAATCCGATCGCCG
CCATCTGGTCGGGCGCAATGATGCTGGAGCATCTCGGAGAACCGGCTGCCGCAGCAAGGGTGATGGCATCAATCGAGGCG
ACGACGGCACGCGGCATCGGCTCGATTCCCGGCAAGGACAAGACCGACGCGATCACGGCATCGGTGCTTTCGGCACTCGG
CTGA

Upstream 100 bases:

>100_bases
CGGCTACAAGCAGTCCGGCATCGGCAAGGATCTCGGCCGCGACGCCTATCTCGCCAACCGCAAGAGCAAGAGTGTGCTCA
TCAGCCTGTGAGGATCTGCG

Downstream 100 bases:

>100_bases
TCAATGGAGAACAGAATGAACGGATTGAGGGATAGCAGCCTGCTTCGTCAGCAGGGACTGATCGACGGAGAATGGCGGGG
GGCCGCGGCCGGGCACACGA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: TDH; D-malate dehydrogenase [decarboxylating] [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKTYKIALLPGDGIGRDVTAAAWAVLEKAARLNGFSLDATSYPWSCDYYLENGSMMPADGIEILRSFDAILLGAVGWPRK
VPDSVSLHGLLLPIRKAFVQYANIRPHRLLPGVQGPLRSDGFNILCIRENTEGEYSGAGGRVHQGTDNEVAVETSIFTRK
GVERILRFGFEQARARRGKLASVTKSNAQKYSMVFWDEITQRLSAEYPDVEVTSYHIDAMAARMVMAPDSLDVVVASNLF
GDILTDLGAAIQGGLGFAASANINPDRSAPSMFEPVHGSAPDIADLGIANPIAAIWSGAMMLEHLGEPAAAARVMASIEA
TTARGIGSIPGKDKTDAITASVLSALG

Sequences:

>Translated_347_residues
MKTYKIALLPGDGIGRDVTAAAWAVLEKAARLNGFSLDATSYPWSCDYYLENGSMMPADGIEILRSFDAILLGAVGWPRK
VPDSVSLHGLLLPIRKAFVQYANIRPHRLLPGVQGPLRSDGFNILCIRENTEGEYSGAGGRVHQGTDNEVAVETSIFTRK
GVERILRFGFEQARARRGKLASVTKSNAQKYSMVFWDEITQRLSAEYPDVEVTSYHIDAMAARMVMAPDSLDVVVASNLF
GDILTDLGAAIQGGLGFAASANINPDRSAPSMFEPVHGSAPDIADLGIANPIAAIWSGAMMLEHLGEPAAAARVMASIEA
TTARGIGSIPGKDKTDAITASVLSALG
>Mature_347_residues
MKTYKIALLPGDGIGRDVTAAAWAVLEKAARLNGFSLDATSYPWSCDYYLENGSMMPADGIEILRSFDAILLGAVGWPRK
VPDSVSLHGLLLPIRKAFVQYANIRPHRLLPGVQGPLRSDGFNILCIRENTEGEYSGAGGRVHQGTDNEVAVETSIFTRK
GVERILRFGFEQARARRGKLASVTKSNAQKYSMVFWDEITQRLSAEYPDVEVTSYHIDAMAARMVMAPDSLDVVVASNLF
GDILTDLGAAIQGGLGFAASANINPDRSAPSMFEPVHGSAPDIADLGIANPIAAIWSGAMMLEHLGEPAAAARVMASIEA
TTARGIGSIPGKDKTDAITASVLSALG

Specific function: Unknown

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=327, Percent_Identity=34.5565749235474, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI4758582, Length=320, Percent_Identity=27.5, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI28178838, Length=320, Percent_Identity=27.5, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI28178816, Length=310, Percent_Identity=27.4193548387097, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI28178821, Length=310, Percent_Identity=27.4193548387097, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI28178819, Length=153, Percent_Identity=30.718954248366, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1788101, Length=335, Percent_Identity=52.5373134328358, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI87081683, Length=336, Percent_Identity=39.2857142857143, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI1787381, Length=366, Percent_Identity=25.9562841530055, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71986051, Length=345, Percent_Identity=33.3333333333333, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25144293, Length=347, Percent_Identity=29.1066282420749, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17505779, Length=305, Percent_Identity=29.1803278688525, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17550882, Length=307, Percent_Identity=27.3615635179153, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6322097, Length=362, Percent_Identity=33.1491712707182, Blast_Score=159, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6324709, Length=355, Percent_Identity=32.3943661971831, Blast_Score=155, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6324291, Length=351, Percent_Identity=30.4843304843305, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319830, Length=367, Percent_Identity=30.5177111716621, Blast_Score=133, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24643268, Length=355, Percent_Identity=29.8591549295775, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24643270, Length=355, Percent_Identity=29.8591549295775, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24661184, Length=324, Percent_Identity=31.7901234567901, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI161078639, Length=258, Percent_Identity=28.2945736434109, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI161078633, Length=258, Percent_Identity=28.2945736434109, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24650122, Length=258, Percent_Identity=28.2945736434109, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI161078637, Length=258, Percent_Identity=28.2945736434109, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI161078635, Length=258, Percent_Identity=28.2945736434109, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI281362242, Length=310, Percent_Identity=27.0967741935484, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648872, Length=310, Percent_Identity=27.0967741935484, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI20130355, Length=326, Percent_Identity=24.5398773006135, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.93; =4.1.1.73; =1.1.1.83 [H]

Molecular weight: Translated: 37007; Mature: 37007

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYKIALLPGDGIGRDVTAAAWAVLEKAARLNGFSLDATSYPWSCDYYLENGSMMPADG
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEEECCCCCCHHH
IEILRSFDAILLGAVGWPRKVPDSVSLHGLLLPIRKAFVQYANIRPHRLLPGVQGPLRSD
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCC
GFNILCIRENTEGEYSGAGGRVHQGTDNEVAVETSIFTRKGVERILRFGFEQARARRGKL
CEEEEEEEECCCCCCCCCCCCEECCCCCCEEEEHHHHHHHHHHHHHHHCHHHHHHHCCCH
ASVTKSNAQKYSMVFWDEITQRLSAEYPDVEVTSYHIDAMAARMVMAPDSLDVVVASNLF
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHCCCCCCCEEEHHHHH
GDILTDLGAAIQGGLGFAASANINPDRSAPSMFEPVHGSAPDIADLGIANPIAAIWSGAM
HHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHCCCCCCCCCHHHCCCCCHHHHHHHHHH
MLEHLGEPAAAARVMASIEATTARGIGSIPGKDKTDAITASVLSALG
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKTYKIALLPGDGIGRDVTAAAWAVLEKAARLNGFSLDATSYPWSCDYYLENGSMMPADG
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEEECCCCCCHHH
IEILRSFDAILLGAVGWPRKVPDSVSLHGLLLPIRKAFVQYANIRPHRLLPGVQGPLRSD
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCC
GFNILCIRENTEGEYSGAGGRVHQGTDNEVAVETSIFTRKGVERILRFGFEQARARRGKL
CEEEEEEEECCCCCCCCCCCCEECCCCCCEEEEHHHHHHHHHHHHHHHCHHHHHHHCCCH
ASVTKSNAQKYSMVFWDEITQRLSAEYPDVEVTSYHIDAMAARMVMAPDSLDVVVASNLF
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHCCCCCCCEEEHHHHH
GDILTDLGAAIQGGLGFAASANINPDRSAPSMFEPVHGSAPDIADLGIANPIAAIWSGAM
HHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHCCCCCCCCCHHHCCCCCHHHHHHHHHH
MLEHLGEPAAAARVMASIEATTARGIGSIPGKDKTDAITASVLSALG
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]