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The map label for this gene is sigK [H]

Identifier: 116249245

GI number: 116249245

Start: 632100

End: 632675

Strand: Direct

Name: sigK [H]

Synonym: pRL120580

Alternate gene names: 116249245

Gene position: 632100-632675 (Clockwise)

Preceding gene: 116249244

Following gene: 116249246

Centisome position: 72.65

GC content: 51.56

Gene sequence:

>576_bases
TTGGAGGAAGAGAAGCAAAAATTGGATGCCGCACGGATCAATTCATTGATGTCGGCCGTGGGAAAGTCGCGCGACATCGA
CTCATTCGAAATTCTGTACAGGCACTTTTGCCCACGCGTCCGTTCTTACATGGCCCGTCTGACGAGAGGCAATCGCATAC
TCGCTGAGGAACTGACCCAGGAAACCATGATGCGCGTCTGGTATAAGGCGGCGCTGTTCGATTCATCAAAGGCGCAGGCA
TCGACCTGGATATTTGCGATTGCGAGAAATCAAATGATCGATGCTGCCCGCAAAGGTGCCCATCCCGAATTCGACGTTAA
CGACCCCGCATTTGTGCCAGATGAGATGGAAGCAGCGGACGTCAAGATTCAGCGGCAACAGAACGCGCAGGAGCTGCATA
ATGCGATGCTCACATTGAAACAGAAGTACGTGGAAGTCCTTCGGATGTCATTCTTCGACGGTCTGACCCACACAATGATA
GCAGAAAGACTAAACCTCCCACTTGGAACGGTAAAATCGAGGATACGGCTTGCATGCGAGAAATTGCGGATGGCGATTCA
GGACGATGCCAAATGA

Upstream 100 bases:

>100_bases
CATGAACATGCGATTACCGTTCCGATCCGGACTACGTCGAGGGACCCGTGCTAAGGCAGTCGCCTGGCACTTGCCGGGTT
TGGAACGAAAGGTCGGCGCC

Downstream 100 bases:

>100_bases
TTGAACACCATCTGGATGACGACATGCTCCTGCACTACTCCGCCGGGACACTGGCGGAGGGTTGGAGCGTCGCAGTTGCG
ACCCACCTCGCACTTTGCCC

Product: putative RNA polymerase ECF-type sigma factor

Products: NA

Alternate protein names: Sigma-K factor [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MEEEKQKLDAARINSLMSAVGKSRDIDSFEILYRHFCPRVRSYMARLTRGNRILAEELTQETMMRVWYKAALFDSSKAQA
STWIFAIARNQMIDAARKGAHPEFDVNDPAFVPDEMEAADVKIQRQQNAQELHNAMLTLKQKYVEVLRMSFFDGLTHTMI
AERLNLPLGTVKSRIRLACEKLRMAIQDDAK

Sequences:

>Translated_191_residues
MEEEKQKLDAARINSLMSAVGKSRDIDSFEILYRHFCPRVRSYMARLTRGNRILAEELTQETMMRVWYKAALFDSSKAQA
STWIFAIARNQMIDAARKGAHPEFDVNDPAFVPDEMEAADVKIQRQQNAQELHNAMLTLKQKYVEVLRMSFFDGLTHTMI
AERLNLPLGTVKSRIRLACEKLRMAIQDDAK
>Mature_191_residues
MEEEKQKLDAARINSLMSAVGKSRDIDSFEILYRHFCPRVRSYMARLTRGNRILAEELTQETMMRVWYKAALFDSSKAQA
STWIFAIARNQMIDAARKGAHPEFDVNDPAFVPDEMEAADVKIQRQQNAQELHNAMLTLKQKYVEVLRMSFFDGLTHTMI
AERLNLPLGTVKSRIRLACEKLRMAIQDDAK

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 22027; Mature: 22027

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEEKQKLDAARINSLMSAVGKSRDIDSFEILYRHFCPRVRSYMARLTRGNRILAEELTQ
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ETMMRVWYKAALFDSSKAQASTWIFAIARNQMIDAARKGAHPEFDVNDPAFVPDEMEAAD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
VKIQRQQNAQELHNAMLTLKQKYVEVLRMSFFDGLTHTMIAERLNLPLGTVKSRIRLACE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KLRMAIQDDAK
HHHHHHHCCCC
>Mature Secondary Structure
MEEEKQKLDAARINSLMSAVGKSRDIDSFEILYRHFCPRVRSYMARLTRGNRILAEELTQ
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ETMMRVWYKAALFDSSKAQASTWIFAIARNQMIDAARKGAHPEFDVNDPAFVPDEMEAAD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
VKIQRQQNAQELHNAMLTLKQKYVEVLRMSFFDGLTHTMIAERLNLPLGTVKSRIRLACE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
KLRMAIQDDAK
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA