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The map label for this gene is bfrA [H]

Identifier: 116249223

GI number: 116249223

Start: 607912

End: 609612

Strand: Direct

Name: bfrA [H]

Synonym: pRL120559

Alternate gene names: 116249223

Gene position: 607912-609612 (Clockwise)

Preceding gene: 116249222

Following gene: 116249224

Centisome position: 69.87

GC content: 64.49

Gene sequence:

>1701_bases
ATGTCTCTGCAGGCAAAATCCGAAACGTCAGCACCTGAAACCATTGAAGCGGAGCTGCCTGAAGGCACGGTGCTGCATCT
CTGGCTGAAGGCGCGCCATGCCGGCGTCGAGGCGAAGCTCTTCGTCGCCGTTGATGGCAACGACATCGGCGAGCCCTCGA
CCCGCCGCACGGGGGAATTTGAGTTCTTCGCTGTGACGCTCGCGAAAGGCGGCCGTGCCACACTGTCCTATGATGCGACA
ACCACAGCGCTCTCGGTCGCTTACGCCTTTCGGCCGGAAACCGTGATGAAGGAGGGCATCCGCGTCATGCACAGCGATGC
CCGCACCGCCGCCCCCGACGTGCCCGACAGCTACCATTTCCGCCCGCCCTTCGGCTGGATGAACGATCCGAACGGTTTTG
GACGGTTCGGCGGAAACGCTCACCTCTTCTACCAGCATTATCCTCATGAGCCGCGCTGGAACACTATGCACTGGGGCCAT
GCCGTCTCGAAGGATTTCGTCCGCTGGAGGCATCTGCCCATGTTCCTCTTCCCGGCGGCGCATCTATCGGAAAAGGACGA
TGGCCGCGGCGGTGCCTTCTCCGGCTCGGCGATCCCCGGCTCCGGCCCGGAAGGCGAGGAGATCCGGGTCTTCTACACTG
AGCATGTGCGTGACCGGCAACCGGAAGAGCAAATCCAGCTTTCCGCGGTCAGCCGCGACGGCATTGTCGCTGGCCCGTCG
GAAATCGTGATGCCGCTGCGCCCGGAAGGCTTGAACCTCACCACCGATTTCCGCGACCCCTATGTCTTCAAAGCCCCGGA
CGGGCGCTGGAAGATGCTGCTTGGCAGCCGCGACAGGCAGGGCGGCGTCGTACTGCTCTACGAAACCGCAGACGCGCAAG
GCGCCGATGGCTGGACCTTCCTTAGCATCCTCCATCGTGAGGACGGTTTCGGCATGACGGCGGCGGAATGCCCCTGCATG
GTGCCGCTTTCCGGCAAAAACGCAGAACCCCGCTGGGCGCTGATCTTCGGTCTGCTCACCAGCCGCGACCCGGCCACCGG
CCGCCGCAACCTCACCTCCGTCACCGTCGGCGGTTTCGATGGTCGCACCTTCACTGCGGAGTTCGTGCAGGAGTTGGATT
TCGGTTCGGATGCCTATGCCTTCCAGGCCTTCGTCGATGGCGACGAGCCGGTCGGCATCGCCTGGCTCGCCAACTGGACG
GATTTTTCCAAGAAGGACGACTTCCCGACGACCATGACGCTGCCGCGCCGCATGCTTCTCGACGGCGACACCGTGCTGAC
CCCGCCGGTCGCAGCCGTCGAAAGCCTGCGTCACCAGTTGCTGGACGACACCGCGCTTGCCGCCGGCAAGACCGTGCCGC
TCGGCACCGGCGCCGTCGAGATCGTGCTTGATCTCACCGCGCCGGGCGCCGCCTTCGATCTCACCTTCGATCATCCAGAT
GTCGATCTAGGCGTGAAACTCGATGCCGATGGTCTGGCGATTGTCTTCGACGCCCGCACCGGTATGACGCCGCCACGTTA
CGTCGCCACCGGCGCGAAACCGTCCAGCCTGCGCATCTTCCTCGATGCCGGCTCCATCGAGGTCTTCGCTGACAACGGCC
GCTGGACGGGGTCCAAACGCATTCCGGGCTTTGCCGCCGCACGTTCGGCAACGCTCGCCGGCGCCGTCGCCGGGGCCGGC
GTCTGGCAATTGAAACTGTGA

Upstream 100 bases:

>100_bases
CCGCCTCGGTGCTGATGATGATCCCGGTCCTCATTCTCTATCTGGCTTTCCAGCGCTGGTTCGTCAGTTCATTCGTCGGC
TCCGCCGTGAAAGGTTGATC

Downstream 100 bases:

>100_bases
GAGAGGACAGACAATGGCATCGGTAGCGATCGACCGGGTTCAGAAACAATACGGCGCGGCCTCCGTTCTCCACGGAGTTT
CCGCAGATATCGAGGACGGT

Product: putative glycosyl hydrolase

Products: NA

Alternate protein names: Invertase; Sucrase [H]

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MSLQAKSETSAPETIEAELPEGTVLHLWLKARHAGVEAKLFVAVDGNDIGEPSTRRTGEFEFFAVTLAKGGRATLSYDAT
TTALSVAYAFRPETVMKEGIRVMHSDARTAAPDVPDSYHFRPPFGWMNDPNGFGRFGGNAHLFYQHYPHEPRWNTMHWGH
AVSKDFVRWRHLPMFLFPAAHLSEKDDGRGGAFSGSAIPGSGPEGEEIRVFYTEHVRDRQPEEQIQLSAVSRDGIVAGPS
EIVMPLRPEGLNLTTDFRDPYVFKAPDGRWKMLLGSRDRQGGVVLLYETADAQGADGWTFLSILHREDGFGMTAAECPCM
VPLSGKNAEPRWALIFGLLTSRDPATGRRNLTSVTVGGFDGRTFTAEFVQELDFGSDAYAFQAFVDGDEPVGIAWLANWT
DFSKKDDFPTTMTLPRRMLLDGDTVLTPPVAAVESLRHQLLDDTALAAGKTVPLGTGAVEIVLDLTAPGAAFDLTFDHPD
VDLGVKLDADGLAIVFDARTGMTPPRYVATGAKPSSLRIFLDAGSIEVFADNGRWTGSKRIPGFAAARSATLAGAVAGAG
VWQLKL

Sequences:

>Translated_566_residues
MSLQAKSETSAPETIEAELPEGTVLHLWLKARHAGVEAKLFVAVDGNDIGEPSTRRTGEFEFFAVTLAKGGRATLSYDAT
TTALSVAYAFRPETVMKEGIRVMHSDARTAAPDVPDSYHFRPPFGWMNDPNGFGRFGGNAHLFYQHYPHEPRWNTMHWGH
AVSKDFVRWRHLPMFLFPAAHLSEKDDGRGGAFSGSAIPGSGPEGEEIRVFYTEHVRDRQPEEQIQLSAVSRDGIVAGPS
EIVMPLRPEGLNLTTDFRDPYVFKAPDGRWKMLLGSRDRQGGVVLLYETADAQGADGWTFLSILHREDGFGMTAAECPCM
VPLSGKNAEPRWALIFGLLTSRDPATGRRNLTSVTVGGFDGRTFTAEFVQELDFGSDAYAFQAFVDGDEPVGIAWLANWT
DFSKKDDFPTTMTLPRRMLLDGDTVLTPPVAAVESLRHQLLDDTALAAGKTVPLGTGAVEIVLDLTAPGAAFDLTFDHPD
VDLGVKLDADGLAIVFDARTGMTPPRYVATGAKPSSLRIFLDAGSIEVFADNGRWTGSKRIPGFAAARSATLAGAVAGAG
VWQLKL
>Mature_565_residues
SLQAKSETSAPETIEAELPEGTVLHLWLKARHAGVEAKLFVAVDGNDIGEPSTRRTGEFEFFAVTLAKGGRATLSYDATT
TALSVAYAFRPETVMKEGIRVMHSDARTAAPDVPDSYHFRPPFGWMNDPNGFGRFGGNAHLFYQHYPHEPRWNTMHWGHA
VSKDFVRWRHLPMFLFPAAHLSEKDDGRGGAFSGSAIPGSGPEGEEIRVFYTEHVRDRQPEEQIQLSAVSRDGIVAGPSE
IVMPLRPEGLNLTTDFRDPYVFKAPDGRWKMLLGSRDRQGGVVLLYETADAQGADGWTFLSILHREDGFGMTAAECPCMV
PLSGKNAEPRWALIFGLLTSRDPATGRRNLTSVTVGGFDGRTFTAEFVQELDFGSDAYAFQAFVDGDEPVGIAWLANWTD
FSKKDDFPTTMTLPRRMLLDGDTVLTPPVAAVESLRHQLLDDTALAAGKTVPLGTGAVEIVLDLTAPGAAFDLTFDHPDV
DLGVKLDADGLAIVFDARTGMTPPRYVATGAKPSSLRIFLDAGSIEVFADNGRWTGSKRIPGFAAARSATLAGAVAGAGV
WQLKL

Specific function: Hydrolysis of sucrose, raffinose, inulin and levan. Specific for the fructose moiety and the beta-anomeric configuration of the glycosidic linkages of its substrates. The enzyme released fructose from sucrose and raffinose, and the fructose polymer inulin

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=439, Percent_Identity=26.879271070615, Blast_Score=115, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 61503; Mature: 61372

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQAKSETSAPETIEAELPEGTVLHLWLKARHAGVEAKLFVAVDGNDIGEPSTRRTGEF
CCCCCCCCCCCCCEEEEECCCCCEEEEEEEHHCCCCEEEEEEEECCCCCCCCCCCCCCCE
EFFAVTLAKGGRATLSYDATTTALSVAYAFRPETVMKEGIRVMHSDARTAAPDVPDSYHF
EEEEEEEECCCCEEEEECCCCEEEEEEEEECCHHHHHHHHHEEECCCCCCCCCCCCCCCC
RPPFGWMNDPNGFGRFGGNAHLFYQHYPHEPRWNTMHWGHAVSKDFVRWRHLPMFLFPAA
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEECCHHHHHHHHHHHCCCEEEECHH
HLSEKDDGRGGAFSGSAIPGSGPEGEEIRVFYTEHVRDRQPEEQIQLSAVSRDGIVAGPS
HCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHEEEEEECCCCEEECHH
EIVMPLRPEGLNLTTDFRDPYVFKAPDGRWKMLLGSRDRQGGVVLLYETADAQGADGWTF
HEEEECCCCCCEEEECCCCCEEEECCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCEEE
LSILHREDGFGMTAAECPCMVPLSGKNAEPRWALIFGLLTSRDPATGRRNLTSVTVGGFD
EEEEECCCCCCCCHHCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCC
GRTFTAEFVQELDFGSDAYAFQAFVDGDEPVGIAWLANWTDFSKKDDFPTTMTLPRRMLL
CCEEHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCEECCCCEEEE
DGDTVLTPPVAAVESLRHQLLDDTALAAGKTVPLGTGAVEIVLDLTAPGAAFDLTFDHPD
CCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCC
VDLGVKLDADGLAIVFDARTGMTPPRYVATGAKPSSLRIFLDAGSIEVFADNGRWTGSKR
CCEEEEECCCCEEEEEECCCCCCCCCEEECCCCCCEEEEEEECCCEEEEECCCEECCCCC
IPGFAAARSATLAGAVAGAGVWQLKL
CCCHHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure 
SLQAKSETSAPETIEAELPEGTVLHLWLKARHAGVEAKLFVAVDGNDIGEPSTRRTGEF
CCCCCCCCCCCCEEEEECCCCCEEEEEEEHHCCCCEEEEEEEECCCCCCCCCCCCCCCE
EFFAVTLAKGGRATLSYDATTTALSVAYAFRPETVMKEGIRVMHSDARTAAPDVPDSYHF
EEEEEEEECCCCEEEEECCCCEEEEEEEEECCHHHHHHHHHEEECCCCCCCCCCCCCCCC
RPPFGWMNDPNGFGRFGGNAHLFYQHYPHEPRWNTMHWGHAVSKDFVRWRHLPMFLFPAA
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEECCHHHHHHHHHHHCCCEEEECHH
HLSEKDDGRGGAFSGSAIPGSGPEGEEIRVFYTEHVRDRQPEEQIQLSAVSRDGIVAGPS
HCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHEEEEEECCCCEEECHH
EIVMPLRPEGLNLTTDFRDPYVFKAPDGRWKMLLGSRDRQGGVVLLYETADAQGADGWTF
HEEEECCCCCCEEEECCCCCEEEECCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCEEE
LSILHREDGFGMTAAECPCMVPLSGKNAEPRWALIFGLLTSRDPATGRRNLTSVTVGGFD
EEEEECCCCCCCCHHCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCC
GRTFTAEFVQELDFGSDAYAFQAFVDGDEPVGIAWLANWTDFSKKDDFPTTMTLPRRMLL
CCEEHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCEECCCCEEEE
DGDTVLTPPVAAVESLRHQLLDDTALAAGKTVPLGTGAVEIVLDLTAPGAAFDLTFDHPD
CCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCC
VDLGVKLDADGLAIVFDARTGMTPPRYVATGAKPSSLRIFLDAGSIEVFADNGRWTGSKR
CCEEEEECCCCEEEEEECCCCCCCCCEEECCCCCCEEEEEEECCCEEEEECCCEECCCCC
IPGFAAARSATLAGAVAGAGVWQLKL
CCCHHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9720201; 10360571 [H]