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The map label for this gene is afr [H]

Identifier: 116249055

GI number: 116249055

Start: 421222

End: 422256

Strand: Reverse

Name: afr [H]

Synonym: pRL120389

Alternate gene names: 116249055

Gene position: 422256-421222 (Counterclockwise)

Preceding gene: 116249056

Following gene: 116249054

Centisome position: 48.53

GC content: 60.48

Gene sequence:

>1035_bases
ATGACGGAATTGAAAGGGGCTTTGATCGGTTGCGGCTTCTTTGCGATCAACCAGATGCATGCCTGGAACGACGTTCGGGG
CGCCGGCATCGTCGCGATCTGCGATCGCGATCCGGAGCGGCTGAAGATCGTCGGCGACCAATTCGGCATCGATCGCCGCT
ATGGCGATGCCGAGGCTTTGTTTGCCGACGGCGGTTTTGATTTCGTCGACATTGCCACGACCGTCGAAAGCCATCGCTTT
CTCGTCGAAATGGCGGCTCGGAACAAAGTGCCGGCTATCTGCCAGAAGCCGTTCGCCAAGACCTTGTCGGACGCGAAGGC
GATGGTCGAGGCGTGTCGCAATGCCGGCATACCGCTGATGGTGCACGAGAATTTTCGCTGGCAGACGCCGATCCAGGCTG
TACGAAAAGCCCTCGATTCAAATGCCATCGGCACGCCCTTCTGGGGCCGTTTCTCCTTCCGTTCGGCTTACGACGTCTTC
TCCGGCCAGCCCTATCTCGCCGAAGGCGAACGGTTCATCATCGAAGACCTCGGCATCCATACGCTCGATATCGCCCGCTA
TATCCTTGGCGACGTTCGATCGCTCACGGCCCGCACCAAGCGGGTCAATCCCGGAATCAAGGGCGAGGATGTCGCAACCA
TTCTGCTCGATCACGAAAACGGCGCCACGTCGGTCGTCGACGTCAGCTACGCCACCAGGCTTTCCAGGGAACCGTTCCCC
GAGACCTTGATCGAGCTCGACGGTTCGGAGGGCACGATCCGCCTGACCCAGGGTTACGGCCTCGAAATCACCAACGCCAA
GGGCACCACCACGACCGACGTATCTCCGAAGCTGCTGCCATGGGCCTCGCGGCCCTGGCACAACATCCAGGAGAGCGTCT
ACGCGATCCAGCAGCATTGGGTCGATCAGTTGGAGCGCGGCGCGGAACACTCGACCTCAGGGGCCGACAATCTGAAAACT
TTTGCACTCGTCGAGGCTGCCTATGACAGCGCTGCCGAGGGACAGACGATCGACGTCGGAGCGATGCTGCGATGA

Upstream 100 bases:

>100_bases
ATCAAGGGCCTGGTCATCATCATCGCCGTCGTGGTCGACCAGGCGCAGCGCCGCCTGCAGCAGCGCGTCACCCTCATGCA
GATGGCAAAGGCAGGTTGAT

Downstream 100 bases:

>100_bases
CGATCGATGCGTTCCAGCTCTACGGCACTCATCTCGTCGATCCGCCACCGGTTCGCCTGAGAGCCGGAAAATTGGAAGCC
GATCTCACCAACGGCAACCT

Product: GFO/IDH/MocA family oxidoreductase

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MTELKGALIGCGFFAINQMHAWNDVRGAGIVAICDRDPERLKIVGDQFGIDRRYGDAEALFADGGFDFVDIATTVESHRF
LVEMAARNKVPAICQKPFAKTLSDAKAMVEACRNAGIPLMVHENFRWQTPIQAVRKALDSNAIGTPFWGRFSFRSAYDVF
SGQPYLAEGERFIIEDLGIHTLDIARYILGDVRSLTARTKRVNPGIKGEDVATILLDHENGATSVVDVSYATRLSREPFP
ETLIELDGSEGTIRLTQGYGLEITNAKGTTTTDVSPKLLPWASRPWHNIQESVYAIQQHWVDQLERGAEHSTSGADNLKT
FALVEAAYDSAAEGQTIDVGAMLR

Sequences:

>Translated_344_residues
MTELKGALIGCGFFAINQMHAWNDVRGAGIVAICDRDPERLKIVGDQFGIDRRYGDAEALFADGGFDFVDIATTVESHRF
LVEMAARNKVPAICQKPFAKTLSDAKAMVEACRNAGIPLMVHENFRWQTPIQAVRKALDSNAIGTPFWGRFSFRSAYDVF
SGQPYLAEGERFIIEDLGIHTLDIARYILGDVRSLTARTKRVNPGIKGEDVATILLDHENGATSVVDVSYATRLSREPFP
ETLIELDGSEGTIRLTQGYGLEITNAKGTTTTDVSPKLLPWASRPWHNIQESVYAIQQHWVDQLERGAEHSTSGADNLKT
FALVEAAYDSAAEGQTIDVGAMLR
>Mature_343_residues
TELKGALIGCGFFAINQMHAWNDVRGAGIVAICDRDPERLKIVGDQFGIDRRYGDAEALFADGGFDFVDIATTVESHRFL
VEMAARNKVPAICQKPFAKTLSDAKAMVEACRNAGIPLMVHENFRWQTPIQAVRKALDSNAIGTPFWGRFSFRSAYDVFS
GQPYLAEGERFIIEDLGIHTLDIARYILGDVRSLTARTKRVNPGIKGEDVATILLDHENGATSVVDVSYATRLSREPFPE
TLIELDGSEGTIRLTQGYGLEITNAKGTTTTDVSPKLLPWASRPWHNIQESVYAIQQHWVDQLERGAEHSTSGADNLKTF
ALVEAAYDSAAEGQTIDVGAMLR

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 37881; Mature: 37750

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELKGALIGCGFFAINQMHAWNDVRGAGIVAICDRDPERLKIVGDQFGIDRRYGDAEAL
CCCCCCHHEEHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCEE
FADGGFDFVDIATTVESHRFLVEMAARNKVPAICQKPFAKTLSDAKAMVEACRNAGIPLM
EECCCCCEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VHENFRWQTPIQAVRKALDSNAIGTPFWGRFSFRSAYDVFSGQPYLAEGERFIIEDLGIH
EECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEECCCCH
TLDIARYILGDVRSLTARTKRVNPGIKGEDVATILLDHENGATSVVDVSYATRLSREPFP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEEEHHHHHHCCCCCC
ETLIELDGSEGTIRLTQGYGLEITNAKGTTTTDVSPKLLPWASRPWHNIQESVYAIQQHW
HHHHEECCCCCEEEEECCCCEEEECCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VDQLERGAEHSTSGADNLKTFALVEAAYDSAAEGQTIDVGAMLR
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCC
>Mature Secondary Structure 
TELKGALIGCGFFAINQMHAWNDVRGAGIVAICDRDPERLKIVGDQFGIDRRYGDAEAL
CCCCCHHEEHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCEE
FADGGFDFVDIATTVESHRFLVEMAARNKVPAICQKPFAKTLSDAKAMVEACRNAGIPLM
EECCCCCEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VHENFRWQTPIQAVRKALDSNAIGTPFWGRFSFRSAYDVFSGQPYLAEGERFIIEDLGIH
EECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEECCCCH
TLDIARYILGDVRSLTARTKRVNPGIKGEDVATILLDHENGATSVVDVSYATRLSREPFP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCEEEEEHHHHHHCCCCCC
ETLIELDGSEGTIRLTQGYGLEITNAKGTTTTDVSPKLLPWASRPWHNIQESVYAIQQHW
HHHHEECCCCCEEEEECCCCEEEECCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
VDQLERGAEHSTSGADNLKTFALVEAAYDSAAEGQTIDVGAMLR
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA