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The map label for this gene is dhaK1 [H]

Identifier: 116249053

GI number: 116249053

Start: 418303

End: 419307

Strand: Reverse

Name: dhaK1 [H]

Synonym: pRL120387

Alternate gene names: 116249053

Gene position: 419307-418303 (Counterclockwise)

Preceding gene: 116249054

Following gene: 116249052

Centisome position: 48.2

GC content: 62.49

Gene sequence:

>1005_bases
ATGAAGACGAAGAAGCTTATCAATGCCGGCGCAGATGCCGTCGATGAGATGCTGCAAGGGGTGTTGGCAGCGCATCCGCG
CCACCTATACGCCGTGGAGAACATGCCCCGCGCGATCATTGCCAGAAACGGGCCGCGCAGGGGCAAGGTCGGGCTGGTCA
TCGGCGGTGGATCCGGGCACGAACCGACATTTCTGGGGTTTGTCGGCAAAGGCCTCGCAGACGCGGCCGCGATCGGCAAC
GTCTTCGCGTCGCCGCCGCCCGATCCGATCATCGCCTGCGCCATGGCGGTCGACGGCGGCGCCGGTGTCCTGTTCATGTA
TGGCAATTATGCCGGCGACGTGATGAACTTCGACATGGCTGCCGAGATGCTTGCGCTTGACGAGATCGAGGTACGCACCG
TGCTCACCACGGATGACGTGGCGTCTGCCCCTGCTGATCAGCGGGACAGGCGGCGGGGCGTAGCGGGCAACGTCTTCATC
TTCAAGGCTGCCGGTGCGGCCTGCGATCTTCTCTATTCCTTTGACGATGTCGAGCGTGTCGCCCGCCATGCCAATGCGCG
AACCTATACGATGGGTGTCGCGCTTTCGCCCTGTTCGCTGCCCCAGACCCTCCGACCAAACTTCCTGATCGGCGAAGGCG
AGATGGAGATCGGAATGGGCATCCATGGGGAGCCTGGCGTGGCGCGCGAGCCGCTGAGGCCGGCCGACGCGGTGACCGAC
GAACTCCTGGACAGCATTCTTCGCGAAATGAAAGCAGACCGGGGTGATCGTGTCGCGGTGCTCGTCAATTCACTGGGCTC
GACCCCGATGATGGAACTCTACATCATGATGCGGCGCGTGAAGTCCCGGCTCGACGATGCCGGGTTCGTCATCCATACGT
CGCTCGTCGGAAACTACTGCACCTCGCTCGAAATGGCCGGCGCGTCGATCACGGTCATGCATCTCGACGACGAGCTTCAG
CGATTGATCGACCACCCCTGTGATTGCGCGATGTTTGCGAGGTAA

Upstream 100 bases:

>100_bases
CTGCTCGCGCCGTTTGCGACCGCAGCCTTGCCGCTGGCGGATTGACGTCCCACGCGCCAGCCGCATCTATGCAATCGGAG
TTCGTTCCGTGGAGGATTGC

Downstream 100 bases:

>100_bases
TCGATGATGTTTACGACCGAAGACCTGCAGCGCATGTTTGCCGGGATTGCCGAGGATATTGCAGCAGAGAGCGACCATCT
CTGCGCGTTGGACGGCGCCA

Product: putative dihydroxyacetone kinase

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKTKKLINAGADAVDEMLQGVLAAHPRHLYAVENMPRAIIARNGPRRGKVGLVIGGGSGHEPTFLGFVGKGLADAAAIGN
VFASPPPDPIIACAMAVDGGAGVLFMYGNYAGDVMNFDMAAEMLALDEIEVRTVLTTDDVASAPADQRDRRRGVAGNVFI
FKAAGAACDLLYSFDDVERVARHANARTYTMGVALSPCSLPQTLRPNFLIGEGEMEIGMGIHGEPGVAREPLRPADAVTD
ELLDSILREMKADRGDRVAVLVNSLGSTPMMELYIMMRRVKSRLDDAGFVIHTSLVGNYCTSLEMAGASITVMHLDDELQ
RLIDHPCDCAMFAR

Sequences:

>Translated_334_residues
MKTKKLINAGADAVDEMLQGVLAAHPRHLYAVENMPRAIIARNGPRRGKVGLVIGGGSGHEPTFLGFVGKGLADAAAIGN
VFASPPPDPIIACAMAVDGGAGVLFMYGNYAGDVMNFDMAAEMLALDEIEVRTVLTTDDVASAPADQRDRRRGVAGNVFI
FKAAGAACDLLYSFDDVERVARHANARTYTMGVALSPCSLPQTLRPNFLIGEGEMEIGMGIHGEPGVAREPLRPADAVTD
ELLDSILREMKADRGDRVAVLVNSLGSTPMMELYIMMRRVKSRLDDAGFVIHTSLVGNYCTSLEMAGASITVMHLDDELQ
RLIDHPCDCAMFAR
>Mature_334_residues
MKTKKLINAGADAVDEMLQGVLAAHPRHLYAVENMPRAIIARNGPRRGKVGLVIGGGSGHEPTFLGFVGKGLADAAAIGN
VFASPPPDPIIACAMAVDGGAGVLFMYGNYAGDVMNFDMAAEMLALDEIEVRTVLTTDDVASAPADQRDRRRGVAGNVFI
FKAAGAACDLLYSFDDVERVARHANARTYTMGVALSPCSLPQTLRPNFLIGEGEMEIGMGIHGEPGVAREPLRPADAVTD
ELLDSILREMKADRGDRVAVLVNSLGSTPMMELYIMMRRVKSRLDDAGFVIHTSLVGNYCTSLEMAGASITVMHLDDELQ
RLIDHPCDCAMFAR

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=339, Percent_Identity=34.8082595870207, Blast_Score=183, Evalue=2e-46,
Organism=Escherichia coli, GI87081857, Length=347, Percent_Identity=40.9221902017291, Blast_Score=264, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI17565018, Length=325, Percent_Identity=36, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321055, Length=310, Percent_Identity=32.258064516129, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6323570, Length=325, Percent_Identity=32, Blast_Score=136, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 35749; Mature: 35749

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKKLINAGADAVDEMLQGVLAAHPRHLYAVENMPRAIIARNGPRRGKVGLVIGGGSGH
CCCHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCCCCCEEEEEECCCCC
EPTFLGFVGKGLADAAAIGNVFASPPPDPIIACAMAVDGGAGVLFMYGNYAGDVMNFDMA
CCEEEHHHHCCHHHHHHHHHHCCCCCCCHHEEEEEEECCCCEEEEEECCCCCCHHHHHHH
AEMLALDEIEVRTVLTTDDVASAPADQRDRRRGVAGNVFIFKAAGAACDLLYSFDDVERV
HHHHHHCCEEEEEEEECCHHHCCCCCHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHH
ARHANARTYTMGVALSPCSLPQTLRPNFLIGEGEMEIGMGIHGEPGVAREPLRPADAVTD
HHHCCCCEEEEEEEECCCCCCCCCCCCEEEECCCEEECCCCCCCCCCCCCCCCCHHHHHH
ELLDSILREMKADRGDRVAVLVNSLGSTPMMELYIMMRRVKSRLDDAGFVIHTSLVGNYC
HHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHH
TSLEMAGASITVMHLDDELQRLIDHPCDCAMFAR
HHHEECCCEEEEEECCHHHHHHHCCCCCEECCCC
>Mature Secondary Structure
MKTKKLINAGADAVDEMLQGVLAAHPRHLYAVENMPRAIIARNGPRRGKVGLVIGGGSGH
CCCHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCCCCCEEEEEECCCCC
EPTFLGFVGKGLADAAAIGNVFASPPPDPIIACAMAVDGGAGVLFMYGNYAGDVMNFDMA
CCEEEHHHHCCHHHHHHHHHHCCCCCCCHHEEEEEEECCCCEEEEEECCCCCCHHHHHHH
AEMLALDEIEVRTVLTTDDVASAPADQRDRRRGVAGNVFIFKAAGAACDLLYSFDDVERV
HHHHHHCCEEEEEEEECCHHHCCCCCHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHH
ARHANARTYTMGVALSPCSLPQTLRPNFLIGEGEMEIGMGIHGEPGVAREPLRPADAVTD
HHHCCCCEEEEEEEECCCCCCCCCCCCEEEECCCEEECCCCCCCCCCCCCCCCCHHHHHH
ELLDSILREMKADRGDRVAVLVNSLGSTPMMELYIMMRRVKSRLDDAGFVIHTSLVGNYC
HHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHH
TSLEMAGASITVMHLDDELQRLIDHPCDCAMFAR
HHHEECCCEEEEEECCHHHHHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]