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The map label for this gene is gsiB [H]

Identifier: 116249002

GI number: 116249002

Start: 357489

End: 359063

Strand: Direct

Name: gsiB [H]

Synonym: pRL120333

Alternate gene names: 116249002

Gene position: 357489-359063 (Clockwise)

Preceding gene: 116249000

Following gene: 116249003

Centisome position: 41.09

GC content: 58.54

Gene sequence:

>1575_bases
ATGGCCGCAAAAACATTGACGCGGGCAGCGATCGGGCTGCTTGGCACGATTGCTATTCCTGCAATGGCCGCCGCACAGGA
AACCCCGCTGACGATCGGGATGTCGACGACGCCGACGACGCTGGATCCTCATGAAGACAGTTCGGCGCCAAACAATGCGA
CCTCGCGCCATATCTGGGACAGCCTGATAAACCTCACGGGAACGTCGGCAAATGCGCCGGAACTCGCCACCGAATGGAAG
GTCGTCGACCCTACTCACTGGGAATTCAAGCTGCGGAAGGGCGTGAAATTCCATGACGGCAGCGAGTTCAACGCCGATGA
CGTCATCGCTTCGCTTCTGCGTGCCCGCGACAAGCCCAGCCAGAGCTTTGCTTCCTACACCCGCAATATTGTCAATGTCA
CCGCCACGGATCCCTATACCATCGTCGTCGAGACAAAGGTTCCGGATCCGATCCTGCTGAATTCCGTCAGCCGAATTCGC
ATTATCAGCGCCGATTGCAAGGAGGCGCCAGTCCAGGATTTCGACAACGGCAAGTGTGCAATCGGAACCGGGGCCTATTC
CTTCGTCTCCTATTCGCCAGGCAGCAACCTGACCTTGAAGCGCAATGACAGCTATTTCGCAGGCCCCTCGCACTGGTCGA
ACGTCACGCTGCGCTTCCTGCCCGATGACGGCGCCCGTCTGGCATCGCTTCTCTCCAACGAGATCGACCTTATCGAAACC
CTTCCTGCCGATGGAATGGCACGCGTGGAAGCGAGCGATAATCTGCAAGTCATCAACGGCCTGTCTTCCCGCTTCGTTTA
TCTTGGCCTCGATGTCAGCCGCGATGTTTCACCTTTCGTGAAGGCTGCGGATGGTTCCGATCTCGACAAGAACCCGCTCA
AGGATGAACGGGTGCGTCGGGCAATGCTGATGTCGATCAACCGGCCGGCAATCGTCGACCGCGTCATGCAGAAGAACGGC
ACGGTGGCCGACCAATTCGTCACCCAAGGCTATGCGGGCTATTCCGGAAAAGTCGAGAAGGTGGGTTATGATCCCGCCGC
CGCCAAGGCGCTTTTGGCCGAGGCCGGTTATCCCGATGGCTTCAGCCTGACCCTGCACGGTCCCTCTGGCCGTTACGTCA
AGGATGCCGAAGTTCTGCAGGCGGTCGGCCAGATGTTCACACGCATCGGCATCAAATCGAAAGTCGAAGTCCTGCCCTGG
TCGATGTATTCGGAAGCCTATTCAAAGGGCACCTACAGCACCTATTTCGGCTCCTGGGGTGTGAACACCGGCGAAACCAC
CAATCCGACTGTCGCGCTCGTCGCCACGCGCGACGAGAAAAAGGGGACCGGCAGATATAACGGCGGCGGCGTGTCCGATC
CGAAGATCGACGAGGTACTGGCCAAGGCGAGTTCGACACTCGATGAAGCTGCACGGGCGCCCCTTCTCGAGGAACTGTCG
ACCGAGACCTTCAACAACCTCTGGCTCCTCCCGATGCATTACGAAAACGTCGTGCTGGGTGCAAAGAAGACCGTGTCCTA
CACCCCGCGCGGTGACAAATATACGCTCGCTTACGACGTGAAGCCGGCGGATTAA

Upstream 100 bases:

>100_bases
CCTTGAGGCCCCCTTACCAATTTGTGTCAAGAGTGGACGGGAACCTGCTCGCACCGACCAAGCAAGGTCGGCCCAAAAAC
GCTAAACCGGGATGGAAGAA

Downstream 100 bases:

>100_bases
TGCATGTCGTCCGAAAGCGTGCAGCGGTTTTGGGACAAATAAAGATTTGAAGTGCGTCGCATCGATCCAATCGATGCGGC
GCGGTTTAAAAAGAGCAGCA

Product: putative substrate-binding component of ABC transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MAAKTLTRAAIGLLGTIAIPAMAAAQETPLTIGMSTTPTTLDPHEDSSAPNNATSRHIWDSLINLTGTSANAPELATEWK
VVDPTHWEFKLRKGVKFHDGSEFNADDVIASLLRARDKPSQSFASYTRNIVNVTATDPYTIVVETKVPDPILLNSVSRIR
IISADCKEAPVQDFDNGKCAIGTGAYSFVSYSPGSNLTLKRNDSYFAGPSHWSNVTLRFLPDDGARLASLLSNEIDLIET
LPADGMARVEASDNLQVINGLSSRFVYLGLDVSRDVSPFVKAADGSDLDKNPLKDERVRRAMLMSINRPAIVDRVMQKNG
TVADQFVTQGYAGYSGKVEKVGYDPAAAKALLAEAGYPDGFSLTLHGPSGRYVKDAEVLQAVGQMFTRIGIKSKVEVLPW
SMYSEAYSKGTYSTYFGSWGVNTGETTNPTVALVATRDEKKGTGRYNGGGVSDPKIDEVLAKASSTLDEAARAPLLEELS
TETFNNLWLLPMHYENVVLGAKKTVSYTPRGDKYTLAYDVKPAD

Sequences:

>Translated_524_residues
MAAKTLTRAAIGLLGTIAIPAMAAAQETPLTIGMSTTPTTLDPHEDSSAPNNATSRHIWDSLINLTGTSANAPELATEWK
VVDPTHWEFKLRKGVKFHDGSEFNADDVIASLLRARDKPSQSFASYTRNIVNVTATDPYTIVVETKVPDPILLNSVSRIR
IISADCKEAPVQDFDNGKCAIGTGAYSFVSYSPGSNLTLKRNDSYFAGPSHWSNVTLRFLPDDGARLASLLSNEIDLIET
LPADGMARVEASDNLQVINGLSSRFVYLGLDVSRDVSPFVKAADGSDLDKNPLKDERVRRAMLMSINRPAIVDRVMQKNG
TVADQFVTQGYAGYSGKVEKVGYDPAAAKALLAEAGYPDGFSLTLHGPSGRYVKDAEVLQAVGQMFTRIGIKSKVEVLPW
SMYSEAYSKGTYSTYFGSWGVNTGETTNPTVALVATRDEKKGTGRYNGGGVSDPKIDEVLAKASSTLDEAARAPLLEELS
TETFNNLWLLPMHYENVVLGAKKTVSYTPRGDKYTLAYDVKPAD
>Mature_523_residues
AAKTLTRAAIGLLGTIAIPAMAAAQETPLTIGMSTTPTTLDPHEDSSAPNNATSRHIWDSLINLTGTSANAPELATEWKV
VDPTHWEFKLRKGVKFHDGSEFNADDVIASLLRARDKPSQSFASYTRNIVNVTATDPYTIVVETKVPDPILLNSVSRIRI
ISADCKEAPVQDFDNGKCAIGTGAYSFVSYSPGSNLTLKRNDSYFAGPSHWSNVTLRFLPDDGARLASLLSNEIDLIETL
PADGMARVEASDNLQVINGLSSRFVYLGLDVSRDVSPFVKAADGSDLDKNPLKDERVRRAMLMSINRPAIVDRVMQKNGT
VADQFVTQGYAGYSGKVEKVGYDPAAAKALLAEAGYPDGFSLTLHGPSGRYVKDAEVLQAVGQMFTRIGIKSKVEVLPWS
MYSEAYSKGTYSTYFGSWGVNTGETTNPTVALVATRDEKKGTGRYNGGGVSDPKIDEVLAKASSTLDEAARAPLLEELST
ETFNNLWLLPMHYENVVLGAKKTVSYTPRGDKYTLAYDVKPAD

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=516, Percent_Identity=25.968992248062, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1789966, Length=500, Percent_Identity=26.2, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI87081878, Length=488, Percent_Identity=25.2049180327869, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1789887, Length=542, Percent_Identity=21.5867158671587, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1787762, Length=500, Percent_Identity=21.6, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787495, Length=540, Percent_Identity=22.037037037037, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787551, Length=456, Percent_Identity=22.1491228070175, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1789397, Length=492, Percent_Identity=20.7317073170732, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1786256, Length=172, Percent_Identity=29.6511627906977, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56730; Mature: 56599

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKTLTRAAIGLLGTIAIPAMAAAQETPLTIGMSTTPTTLDPHEDSSAPNNATSRHIWD
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHH
SLINLTGTSANAPELATEWKVVDPTHWEFKLRKGVKFHDGSEFNADDVIASLLRARDKPS
HHHHCCCCCCCCCCHHCCCEEECCCCCEEEECCCCEECCCCCCCHHHHHHHHHHHCCCCH
QSFASYTRNIVNVTATDPYTIVVETKVPDPILLNSVSRIRIISADCKEAPVQDFDNGKCA
HHHHHHHCCEEEEEECCCEEEEEECCCCCHHHHCCCCEEEEEECCCCCCCCCCCCCCCEE
IGTGAYSFVSYSPGSNLTLKRNDSYFAGPSHWSNVTLRFLPDDGARLASLLSNEIDLIET
EECCCEEEEEECCCCCEEEEECCCEECCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHH
LPADGMARVEASDNLQVINGLSSRFVYLGLDVSRDVSPFVKAADGSDLDKNPLKDERVRR
CCCCCEEEEECCCCCCEECCCCCCEEEEEECCCCCCCHHHCCCCCCCCCCCCCHHHHHHH
AMLMSINRPAIVDRVMQKNGTVADQFVTQGYAGYSGKVEKVGYDPAAAKALLAEAGYPDG
HHHHHCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCC
FSLTLHGPSGRYVKDAEVLQAVGQMFTRIGIKSKVEVLPWSMYSEAYSKGTYSTYFGSWG
EEEEEECCCCCEECHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCEEEECCCC
VNTGETTNPTVALVATRDEKKGTGRYNGGGVSDPKIDEVLAKASSTLDEAARAPLLEELS
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHH
TETFNNLWLLPMHYENVVLGAKKTVSYTPRGDKYTLAYDVKPAD
HHHHCCEEEEEEECCCEEECCCCEEEECCCCCEEEEEEECCCCC
>Mature Secondary Structure 
AAKTLTRAAIGLLGTIAIPAMAAAQETPLTIGMSTTPTTLDPHEDSSAPNNATSRHIWD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHH
SLINLTGTSANAPELATEWKVVDPTHWEFKLRKGVKFHDGSEFNADDVIASLLRARDKPS
HHHHCCCCCCCCCCHHCCCEEECCCCCEEEECCCCEECCCCCCCHHHHHHHHHHHCCCCH
QSFASYTRNIVNVTATDPYTIVVETKVPDPILLNSVSRIRIISADCKEAPVQDFDNGKCA
HHHHHHHCCEEEEEECCCEEEEEECCCCCHHHHCCCCEEEEEECCCCCCCCCCCCCCCEE
IGTGAYSFVSYSPGSNLTLKRNDSYFAGPSHWSNVTLRFLPDDGARLASLLSNEIDLIET
EECCCEEEEEECCCCCEEEEECCCEECCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHH
LPADGMARVEASDNLQVINGLSSRFVYLGLDVSRDVSPFVKAADGSDLDKNPLKDERVRR
CCCCCEEEEECCCCCCEECCCCCCEEEEEECCCCCCCHHHCCCCCCCCCCCCCHHHHHHH
AMLMSINRPAIVDRVMQKNGTVADQFVTQGYAGYSGKVEKVGYDPAAAKALLAEAGYPDG
HHHHHCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCC
FSLTLHGPSGRYVKDAEVLQAVGQMFTRIGIKSKVEVLPWSMYSEAYSKGTYSTYFGSWG
EEEEEECCCCCEECHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHCCCCCEEEECCCC
VNTGETTNPTVALVATRDEKKGTGRYNGGGVSDPKIDEVLAKASSTLDEAARAPLLEELS
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHH
TETFNNLWLLPMHYENVVLGAKKTVSYTPRGDKYTLAYDVKPAD
HHHHCCEEEEEEECCCEEECCCCEEEECCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA