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The map label for this gene is potD [C]

Identifier: 116248816

GI number: 116248816

Start: 142879

End: 144114

Strand: Reverse

Name: potD [C]

Synonym: pRL120142

Alternate gene names: 116248816

Gene position: 144114-142879 (Counterclockwise)

Preceding gene: 116248817

Following gene: 116248815

Centisome position: 16.56

GC content: 62.38

Gene sequence:

>1236_bases
ATGACAATTTCGATGGACATGAATCGACGTTCACTGCTGAAGCGATCCGCAGGCGCGATGGCGCTTGCCATCGGCGGCGG
TACGCACTTCCTCTCTTCGCGGGCGGCCTATGCGCAGGCCGCCAGCCTCGCCGGCGAGCAGCTGCGCACGATCGGCCTTT
CGGTGACCGTGCAGGAACGCATCCTGGCCGACTTCAAGGCGGCAAGCGGCGTCGGCACGACCTCCGGCACCGCGGCAACC
TTCCCGGACGCGCAGACACGGATCCTCTCCGGTTCGAAGGACTATGACGTCTGGGAAACGATCGGCGAGCGCCTTCCCTC
CGTCGTCATGACCGACAATGCCGACCCGATCGAGACCGCGGGCCTGAAGAGCTGGTCGAACATCCGCGAGATCTTCACCA
AGCCTGATCCGAAGTTCGACCCCAAGGCGCAGATCGTCGGTCAGATCTGGACGGACGAGACGCAGACGGCGCTCTGGATG
GTACCTTCGGTCTTCAACTTCGATTCGGTCGGTTATAATCCGGAGGTGCTCTCCGACGAGGAAGCCAACAGCTGGACGGC
GATCTTCGACAGCAAGTACAAGGGCAAGTCCGGCCTCAACACCGACCCGCTGACAGCCTTCGGCCAGGCGGTCATGGCGA
TGAACACGCTTGGCCTCTCCAACGTCAAGAACCCCGGCAATCCGACCGCCGCGCAAATCGACGAAGCCGCGAAGTTCCTG
ATCTCGAAGAAGAAGGAAGGCCAGTTCCGCGCTCTCTGGGGCGATTTCGGCGAACTCGTCAACTTCCTGGCATCCGGCGA
AATCGTCGTCTGCGACGCCTGGCAGCCGGCGGTCATGGCCGTCAAGGCACAGGGAAAGCCCTGCAAATACGCCATCCCGA
AGGAAGGCTATCGCGCCTGGTCGATCGGCAACACCATGCTGAAGGGCACGCCGAACAAGGAAGCCGTCGTCGCCTATGCA
GATTACTGGCTGTCGGGCGAACCAGGCATCACGGTGTCCGAGCAGGGCTATTATTCGCCGACCACCAACATCAAGGAGAA
GATGGCGCCGGAGAAATATGCCTTCTGGTACGAAGGCAAGCCGTGGGTCGGTGCGGCTGAGCGCGGCATCAAGGAGGGCG
ATCTGCGCGACGGCGGTTCGCTCGAGACCCGCGCCGCAAACGTCGCCTACTGGCACCAGTGGCCGGACGAATACGACCAC
CTCATCCAGAAGTGGGACGAATTCCTCAGCGCCTGA

Upstream 100 bases:

>100_bases
CGGGCGCGTCAGGACCGATCCCGTCCTGACGCCGAGGCTGGACGAGAACGATGGGTGGTCATGAAGGCCATCCCGGGGAA
CAAAAAACCGGAGAACGACA

Downstream 100 bases:

>100_bases
TGCGTTACCCCCAGCGCCCGGGCATCCCACGTGAGATGCGGTGAGGGCGCCACCTGGCAGGCGGCTCCGGTCGCCTGTCC
TTTAACCAGCAACCCGGAAT

Product: putative substrate-binding component of ABC transporter

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: Spermidine/Putrescine ABC Transporter; Signal Peptide Prediction; Tat Pathway Signal Sequence Domain Protein; Signal Peptide; Conserved Protein

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MTISMDMNRRSLLKRSAGAMALAIGGGTHFLSSRAAYAQAASLAGEQLRTIGLSVTVQERILADFKAASGVGTTSGTAAT
FPDAQTRILSGSKDYDVWETIGERLPSVVMTDNADPIETAGLKSWSNIREIFTKPDPKFDPKAQIVGQIWTDETQTALWM
VPSVFNFDSVGYNPEVLSDEEANSWTAIFDSKYKGKSGLNTDPLTAFGQAVMAMNTLGLSNVKNPGNPTAAQIDEAAKFL
ISKKKEGQFRALWGDFGELVNFLASGEIVVCDAWQPAVMAVKAQGKPCKYAIPKEGYRAWSIGNTMLKGTPNKEAVVAYA
DYWLSGEPGITVSEQGYYSPTTNIKEKMAPEKYAFWYEGKPWVGAAERGIKEGDLRDGGSLETRAANVAYWHQWPDEYDH
LIQKWDEFLSA

Sequences:

>Translated_411_residues
MTISMDMNRRSLLKRSAGAMALAIGGGTHFLSSRAAYAQAASLAGEQLRTIGLSVTVQERILADFKAASGVGTTSGTAAT
FPDAQTRILSGSKDYDVWETIGERLPSVVMTDNADPIETAGLKSWSNIREIFTKPDPKFDPKAQIVGQIWTDETQTALWM
VPSVFNFDSVGYNPEVLSDEEANSWTAIFDSKYKGKSGLNTDPLTAFGQAVMAMNTLGLSNVKNPGNPTAAQIDEAAKFL
ISKKKEGQFRALWGDFGELVNFLASGEIVVCDAWQPAVMAVKAQGKPCKYAIPKEGYRAWSIGNTMLKGTPNKEAVVAYA
DYWLSGEPGITVSEQGYYSPTTNIKEKMAPEKYAFWYEGKPWVGAAERGIKEGDLRDGGSLETRAANVAYWHQWPDEYDH
LIQKWDEFLSA
>Mature_410_residues
TISMDMNRRSLLKRSAGAMALAIGGGTHFLSSRAAYAQAASLAGEQLRTIGLSVTVQERILADFKAASGVGTTSGTAATF
PDAQTRILSGSKDYDVWETIGERLPSVVMTDNADPIETAGLKSWSNIREIFTKPDPKFDPKAQIVGQIWTDETQTALWMV
PSVFNFDSVGYNPEVLSDEEANSWTAIFDSKYKGKSGLNTDPLTAFGQAVMAMNTLGLSNVKNPGNPTAAQIDEAAKFLI
SKKKEGQFRALWGDFGELVNFLASGEIVVCDAWQPAVMAVKAQGKPCKYAIPKEGYRAWSIGNTMLKGTPNKEAVVAYAD
YWLSGEPGITVSEQGYYSPTTNIKEKMAPEKYAFWYEGKPWVGAAERGIKEGDLRDGGSLETRAANVAYWHQWPDEYDHL
IQKWDEFLSA

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 44952; Mature: 44821

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISMDMNRRSLLKRSAGAMALAIGGGTHFLSSRAAYAQAASLAGEQLRTIGLSVTVQER
CEEECCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHH
ILADFKAASGVGTTSGTAATFPDAQTRILSGSKDYDVWETIGERLPSVVMTDNADPIETA
HHHHHHHHCCCCCCCCCEEECCCCHHHEECCCCCCHHHHHHHHHCCCEEEECCCCCCHHH
GLKSWSNIREIFTKPDPKFDPKAQIVGQIWTDETQTALWMVPSVFNFDSVGYNPEVLSDE
CCCCHHHHHHHHCCCCCCCCCHHHHHEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
EANSWTAIFDSKYKGKSGLNTDPLTAFGQAVMAMNTLGLSNVKNPGNPTAAQIDEAAKFL
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ISKKKEGQFRALWGDFGELVNFLASGEIVVCDAWQPAVMAVKAQGKPCKYAIPKEGYRAW
HHCCCCCCEEEHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCEECCCCCCCEEE
SIGNTMLKGTPNKEAVVAYADYWLSGEPGITVSEQGYYSPTTNIKEKMAPEKYAFWYEGK
ECCCEEECCCCCCCEEEEEEEEEECCCCCEEECCCCCCCCCCCHHHHCCCCCEEEEECCC
PWVGAAERGIKEGDLRDGGSLETRAANVAYWHQWPDEYDHLIQKWDEFLSA
CCCCHHHCCCCCCCCCCCCCCCCEECCEEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TISMDMNRRSLLKRSAGAMALAIGGGTHFLSSRAAYAQAASLAGEQLRTIGLSVTVQER
EEECCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHH
ILADFKAASGVGTTSGTAATFPDAQTRILSGSKDYDVWETIGERLPSVVMTDNADPIETA
HHHHHHHHCCCCCCCCCEEECCCCHHHEECCCCCCHHHHHHHHHCCCEEEECCCCCCHHH
GLKSWSNIREIFTKPDPKFDPKAQIVGQIWTDETQTALWMVPSVFNFDSVGYNPEVLSDE
CCCCHHHHHHHHCCCCCCCCCHHHHHEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
EANSWTAIFDSKYKGKSGLNTDPLTAFGQAVMAMNTLGLSNVKNPGNPTAAQIDEAAKFL
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ISKKKEGQFRALWGDFGELVNFLASGEIVVCDAWQPAVMAVKAQGKPCKYAIPKEGYRAW
HHCCCCCCEEEHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCEECCCCCCCEEE
SIGNTMLKGTPNKEAVVAYADYWLSGEPGITVSEQGYYSPTTNIKEKMAPEKYAFWYEGK
ECCCEEECCCCCCCEEEEEEEEEECCCCCEEECCCCCCCCCCCHHHHCCCCCEEEEECCC
PWVGAAERGIKEGDLRDGGSLETRAANVAYWHQWPDEYDHLIQKWDEFLSA
CCCCHHHCCCCCCCCCCCCCCCCEECCEEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA