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The map label for this gene is groL

Identifier: 114800428

GI number: 114800428

Start: 2031071

End: 2032714

Strand: Direct

Name: groL

Synonym: HNE_1962

Alternate gene names: 114800428

Gene position: 2031071-2032714 (Clockwise)

Preceding gene: 114797690

Following gene: 114799065

Centisome position: 54.82

GC content: 58.82

Gene sequence:

>1644_bases
ATGGCTGCAAAACACGTTGTTTTCGGCGCCGAAGCCCGCGAGCGGATGCTCCGCGGCGTCGACACCCTCGCGAATGCTGT
GAAGGTCACACTCGGCCCTAAAGGCCGTAACGTGGTCATCGAAAAATCGTTCGGCGCTCCGCGCTCCACGAAAGATGGCG
TCACCGTCGCGAAAGAAATCGAACTGGAAGACAAGTTCGAAAATATGGGCGCCCAGATGCTGCGCGAAGTCGCATCGAAG
GCCAATGACACCGCCGGTGACGGCACCACCACTGCCACCGTGCTCGCTCAGGCCATCGTTCGCGAAGGCATGAAGCGCGT
TGCCGCCGGCATGAACCCGATGGACCTTAAGCGCGGCATCTCGAAGGCCGTTGCTGAAGTCGTTTCCGACCTCGCTCATC
ACTCCAAGAAAGTGAAGACGAACGAAGAGATCGCCCAGGTCGGCACCATCTCGGCCAACGGCGAAACCGAAATCGGCCAG
ATGATCGCTGAAGCCATGGCGAAAGTCGGCAACGAAGGCGTCATCACGGTTGAAGAAGCCAAAGCGCTGGAAACCGAACT
CGATGTCGTCGAAGGCATGCAGTTCGACCGCGGCTATATCAGCCCGTACTTCATCACCAACCCGGACAAGATGATCGTTG
AACTCGAAGACGTTCTGATCCTCCTGCACGAGTCGAAGCTGTCGAGCCTCCAGCCGCTGCTTCCGATCCTCGAATCGGTT
GTTCAGTCGCAGAAGCCGCTGCTCATCATCGCTGAAGACGTTGATGGCGAAGCCCTCGCAACTCTGGTGGTCAACAAGCT
GCGTGGCGGCCTGAAAATCGCTGCTGTGAAAGCTCCTGGCTTCGGCGATCGCCGCAAAGCCATGCTGCAGGACCTCGCCG
TCCTTACCGGTGGTCAGGTCATCTCTGAAGACCTCGGCATCAAGCTTGAAAACGTCGGCATGGAAATGCTCGGCAAAGCC
AAACGCGTCTCGATCGACAAAGACAACACCACGATCGTTGATGGCGGCGGCAAGAAGAAAGAGATCGAAGCGCGCGTTTC
GCAAATCCGCAAGCAGATCGATGACACCTCTTCCGACTATGACCGTGAGAAGCTCCAGGAGCGTCTCGCGAAACTGGCCG
GCGGCGTTGCCGTTATCAAAGTTGGCGGCGCAACCGAAGTCGAAGTGAAAGAGCGTAAGGACCGCGTCGACGACGCCCTG
AACGCAACGCGCGCGGCTGTCGAAGAAGGCATCGTGCCAGGTGGCGGCGTTGCCCTGCTCCGTTCTTCCAAGAACATCGA
CGTTGTTGGTCTCAACGACGACGAAAAAGCCGGCATCGACATCGTTCGCAAGGCACTCGAAGCTCCGATCCGTCAGATCG
CAGAGAACGCAGGCGTTGAAGGCTCGGTGGTCGTCAACACGATCCTGAACAACAAGTCGCGCTCTTACGGCTTCAACGCT
CAGACTGAAGAGTATGGCGATCTCGTCGCTATGGGCGTTATCGACCCCGTGAAGGTCGTCCGCTCGGCTCTGCAGAACGC
GGCCTCGATCGCCAGCCTGCTGATCACGACGGAAGCCGGCATTGCCGAAGCTCCGAAGAAAGAGTCAGCCGGCGGCGGTG
GCATGCCCGGCGGTATGGGTGGCATGGGCGGTATGGACTTCTAA

Upstream 100 bases:

>100_bases
CTGATCATGAAGGAAAGCGACATCATGGGCATTCTGGACAAGTAAGTCCGGCCCCGTCCCGCTCCCTGAAACCAAGATAT
AAACACAAGAGGTAACTCAC

Downstream 100 bases:

>100_bases
GTCCCGCCTTTCGCAACCTAGCGAAAACTTCAGCAGCCCTCCGGAGCAATCCGGGGGGCTGTTTACTTCGGGTCATGATT
GCAGGCCCGCTCAGCCCTCC

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MAAKHVVFGAEARERMLRGVDTLANAVKVTLGPKGRNVVIEKSFGAPRSTKDGVTVAKEIELEDKFENMGAQMLREVASK
ANDTAGDGTTTATVLAQAIVREGMKRVAAGMNPMDLKRGISKAVAEVVSDLAHHSKKVKTNEEIAQVGTISANGETEIGQ
MIAEAMAKVGNEGVITVEEAKALETELDVVEGMQFDRGYISPYFITNPDKMIVELEDVLILLHESKLSSLQPLLPILESV
VQSQKPLLIIAEDVDGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLQDLAVLTGGQVISEDLGIKLENVGMEMLGKA
KRVSIDKDNTTIVDGGGKKKEIEARVSQIRKQIDDTSSDYDREKLQERLAKLAGGVAVIKVGGATEVEVKERKDRVDDAL
NATRAAVEEGIVPGGGVALLRSSKNIDVVGLNDDEKAGIDIVRKALEAPIRQIAENAGVEGSVVVNTILNNKSRSYGFNA
QTEEYGDLVAMGVIDPVKVVRSALQNAASIASLLITTEAGIAEAPKKESAGGGGMPGGMGGMGGMDF

Sequences:

>Translated_547_residues
MAAKHVVFGAEARERMLRGVDTLANAVKVTLGPKGRNVVIEKSFGAPRSTKDGVTVAKEIELEDKFENMGAQMLREVASK
ANDTAGDGTTTATVLAQAIVREGMKRVAAGMNPMDLKRGISKAVAEVVSDLAHHSKKVKTNEEIAQVGTISANGETEIGQ
MIAEAMAKVGNEGVITVEEAKALETELDVVEGMQFDRGYISPYFITNPDKMIVELEDVLILLHESKLSSLQPLLPILESV
VQSQKPLLIIAEDVDGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLQDLAVLTGGQVISEDLGIKLENVGMEMLGKA
KRVSIDKDNTTIVDGGGKKKEIEARVSQIRKQIDDTSSDYDREKLQERLAKLAGGVAVIKVGGATEVEVKERKDRVDDAL
NATRAAVEEGIVPGGGVALLRSSKNIDVVGLNDDEKAGIDIVRKALEAPIRQIAENAGVEGSVVVNTILNNKSRSYGFNA
QTEEYGDLVAMGVIDPVKVVRSALQNAASIASLLITTEAGIAEAPKKESAGGGGMPGGMGGMGGMDF
>Mature_546_residues
AAKHVVFGAEARERMLRGVDTLANAVKVTLGPKGRNVVIEKSFGAPRSTKDGVTVAKEIELEDKFENMGAQMLREVASKA
NDTAGDGTTTATVLAQAIVREGMKRVAAGMNPMDLKRGISKAVAEVVSDLAHHSKKVKTNEEIAQVGTISANGETEIGQM
IAEAMAKVGNEGVITVEEAKALETELDVVEGMQFDRGYISPYFITNPDKMIVELEDVLILLHESKLSSLQPLLPILESVV
QSQKPLLIIAEDVDGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLQDLAVLTGGQVISEDLGIKLENVGMEMLGKAK
RVSIDKDNTTIVDGGGKKKEIEARVSQIRKQIDDTSSDYDREKLQERLAKLAGGVAVIKVGGATEVEVKERKDRVDDALN
ATRAAVEEGIVPGGGVALLRSSKNIDVVGLNDDEKAGIDIVRKALEAPIRQIAENAGVEGSVVVNTILNNKSRSYGFNAQ
TEEYGDLVAMGVIDPVKVVRSALQNAASIASLLITTEAGIAEAPKKESAGGGGMPGGMGGMGGMDF

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=528, Percent_Identity=54.5454545454545, Blast_Score=561, Evalue=1e-160,
Organism=Homo sapiens, GI31542947, Length=528, Percent_Identity=54.5454545454545, Blast_Score=561, Evalue=1e-160,
Organism=Homo sapiens, GI5453607, Length=536, Percent_Identity=21.8283582089552, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790586, Length=531, Percent_Identity=66.1016949152542, Blast_Score=686, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=529, Percent_Identity=54.2533081285444, Blast_Score=564, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI193210679, Length=222, Percent_Identity=51.3513513513513, Blast_Score=219, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI17564182, Length=559, Percent_Identity=22.0035778175313, Blast_Score=84, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323288, Length=524, Percent_Identity=58.206106870229, Blast_Score=600, Evalue=1e-172,
Organism=Saccharomyces cerevisiae, GI6322350, Length=559, Percent_Identity=25.2236135957066, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322524, Length=188, Percent_Identity=24.468085106383, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24641193, Length=528, Percent_Identity=56.4393939393939, Blast_Score=585, Evalue=1e-167,
Organism=Drosophila melanogaster, GI24641191, Length=528, Percent_Identity=56.4393939393939, Blast_Score=585, Evalue=1e-167,
Organism=Drosophila melanogaster, GI45550936, Length=530, Percent_Identity=52.8301886792453, Blast_Score=548, Evalue=1e-156,
Organism=Drosophila melanogaster, GI45550132, Length=530, Percent_Identity=52.8301886792453, Blast_Score=548, Evalue=1e-156,
Organism=Drosophila melanogaster, GI45550935, Length=530, Percent_Identity=52.8301886792453, Blast_Score=548, Evalue=1e-156,
Organism=Drosophila melanogaster, GI17864606, Length=555, Percent_Identity=45.7657657657658, Blast_Score=476, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24584129, Length=529, Percent_Identity=38.1852551984877, Blast_Score=349, Evalue=3e-96,
Organism=Drosophila melanogaster, GI19921262, Length=529, Percent_Identity=38.1852551984877, Blast_Score=349, Evalue=3e-96,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_HYPNA (Q0C0T0)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760663.1
- ProteinModelPortal:   Q0C0T0
- SMR:   Q0C0T0
- STRING:   Q0C0T0
- GeneID:   4290372
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_1962
- NMPDR:   fig|228405.5.peg.1891
- TIGR:   HNE_1962
- eggNOG:   COG0459
- HOGENOM:   HBG625289
- OMA:   NSDTSIG
- PhylomeDB:   Q0C0T0
- ProtClustDB:   CLSK2532151
- BioCyc:   HNEP81032:HNE_1962-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 57854; Mature: 57723

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKHVVFGAEARERMLRGVDTLANAVKVTLGPKGRNVVIEKSFGAPRSTKDGVTVAKEI
CCCCCEEECHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCEEHHHC
ELEDKFENMGAQMLREVASKANDTAGDGTTTATVLAQAIVREGMKRVAAGMNPMDLKRGI
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
SKAVAEVVSDLAHHSKKVKTNEEIAQVGTISANGETEIGQMIAEAMAKVGNEGVITVEEA
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHH
KALETELDVVEGMQFDRGYISPYFITNPDKMIVELEDVLILLHESKLSSLQPLLPILESV
HHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHH
VQSQKPLLIIAEDVDGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLQDLAVLTGGQV
HCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHH
ISEDLGIKLENVGMEMLGKAKRVSIDKDNTTIVDGGGKKKEIEARVSQIRKQIDDTSSDY
HHHHHCCEEHHCCHHHHCCHHEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCC
DREKLQERLAKLAGGVAVIKVGGATEVEVKERKDRVDDALNATRAAVEEGIVPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
RSSKNIDVVGLNDDEKAGIDIVRKALEAPIRQIAENAGVEGSVVVNTILNNKSRSYGFNA
ECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCCCCCC
QTEEYGDLVAMGVIDPVKVVRSALQNAASIASLLITTEAGIAEAPKKESAGGGGMPGGMG
CCHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
GMGGMDF
CCCCCCC
>Mature Secondary Structure 
AAKHVVFGAEARERMLRGVDTLANAVKVTLGPKGRNVVIEKSFGAPRSTKDGVTVAKEI
CCCCEEECHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCEEHHHC
ELEDKFENMGAQMLREVASKANDTAGDGTTTATVLAQAIVREGMKRVAAGMNPMDLKRGI
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
SKAVAEVVSDLAHHSKKVKTNEEIAQVGTISANGETEIGQMIAEAMAKVGNEGVITVEEA
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHH
KALETELDVVEGMQFDRGYISPYFITNPDKMIVELEDVLILLHESKLSSLQPLLPILESV
HHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHH
VQSQKPLLIIAEDVDGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLQDLAVLTGGQV
HCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHH
ISEDLGIKLENVGMEMLGKAKRVSIDKDNTTIVDGGGKKKEIEARVSQIRKQIDDTSSDY
HHHHHCCEEHHCCHHHHCCHHEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCC
DREKLQERLAKLAGGVAVIKVGGATEVEVKERKDRVDDALNATRAAVEEGIVPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
RSSKNIDVVGLNDDEKAGIDIVRKALEAPIRQIAENAGVEGSVVVNTILNNKSRSYGFNA
ECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCCCCCC
QTEEYGDLVAMGVIDPVKVVRSALQNAASIASLLITTEAGIAEAPKKESAGGGGMPGGMG
CCHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
GMGGMDF
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA