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The map label for this gene is oprM [H]

Identifier: 114800422

GI number: 114800422

Start: 2186411

End: 2187814

Strand: Reverse

Name: oprM [H]

Synonym: HNE_2110

Alternate gene names: 114800422

Gene position: 2187814-2186411 (Counterclockwise)

Preceding gene: 114799636

Following gene: 114798907

Centisome position: 59.05

GC content: 67.74

Gene sequence:

>1404_bases
ATGATACATCTCAAACTCCCCACGCTCACGGCCATTGCCGCTCTCGCCCTCAGCGCCTGCGCCACCGCGCCCGCCGCCCC
GGACAGCTCCGTTGCTTCGCGCGTTGAAGGCGCCTTGCTCTCGGCAGAAGCGGCCAATGTCGCCGCCACGCCTGTGCCCA
AAGACTGGTGGCGCCTCTATCAGAGCGAGGCCCTCGACGGCCTCGTCACGCAGGCGCTCACCAATAACCGCTCGCTTGCC
GGCGCCGCGGCAAATCTTGAGCAGGTTCGTGCAGGGCTTTCGGAGGCCCGCTCCGCGCTGCTGCCATCCACCACCGCCAC
CGCCGGCGGCCAATATGTCCGTGATCCGAGCTTCGCGCCGGGCGATGTTGAAACCGACGTCTATTCGCTTGGCTTCGAGA
TGGCCTATGAGGTCGATATCTTCGGCCGCGTCAGCTCCAGCGTCGAAGCTGCCCGCCAGGACGCTGCCGCTGCCGAGGCC
GCCTATCGCGGCGCGTCCCTGATGGTCGCGGGTGAAACCGCCCGCGCCTGGATCGATTATTGCGCCGGCAATATCCAGCT
TGAAACCGCCCAGTCGAATTACGGCTTGCAGGAAGAGACGCTCAATCTCACCCGCACCCTGTTCGATGCGGGCCGCGGCA
TCCGTCTGGATGTCGTGCAGGCCGAGGCCGCGCTCCGCCGCGCCGAAGCCGACATCCCGTCCGTGCGCGCCGCCCGTGAC
GCCGCCTTGTTCCGCCTGGCCCGCTTGACTGGCCAGACCCCCGCAGAGATGAGCCAAGCGGTCCCCGCGTGCGAAAGCAT
GCCGCAGCTCGTCTCGCTCCTCCCCACCGGGGAAAGCGCAACCCTCCTTGCGCGCCGTCCGGATGTGCGCCAGGCGGAAC
TTCAGCTTGCCAGCGCCGCCGCCCGCGTCGGTATCGCCACGGCAAACCTCTACCCTTCCGTCAGCATCGGCGGCTCGGTC
TCCACCGCCTCGCTGGGCGGGGCAGGCGACATGTTCAGCGAAGACGGCCTCAGCTTCGGCCTCGGCCCGCTGATCAGCTG
GAACTTCCCGAACGTCGCTGCCGCCCGCGCCCGCCTCGCTGCGGCCGAAGCCGGCGTCGACATCGCCCTCGCCAATTTCG
ACGAGACCTTCCTCACCGCCCTGGAAGAGGCCGAAATCGCCCTCACCAACTACGCGGCCGAGAAAGAGCGCTACGCCGCC
CTGGAAGCTTCCGCCGCTGCGTCGGAAGAAGCCGCCGAACTCGCCCGCACCCGTTACCGGATCGGGTCAGACAACTTCCT
CAACGTCCTCGACGCCGAGCGCACCTTCATCGAAACCCGCACCGCGCTCGCCCAATCCGAAGCGGCAAAGGCCAGCGCCG
AGATCAACGTCTTCATGGCCATGGGTGGCGGCTGGGCTGACTAG

Upstream 100 bases:

>100_bases
TCACGCCGGCTTTCTATGTCATTGGACGGATGATCTCGAAGCGGCTTCCCGGTGCCAGCCGTAAAGACGACACCCACACG
CCGGTCGAAGGAGGCCAGGC

Downstream 100 bases:

>100_bases
CTCTGGCTCCAACCAGTGGCCCAGCACGGCGCGGAACGGCGAAAGCTGTCCCGCGCCGTGTCTTTTTGTGTCCGAGTTTG
TCCTGAAGTGTCCCGTTTTG

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MIHLKLPTLTAIAALALSACATAPAAPDSSVASRVEGALLSAEAANVAATPVPKDWWRLYQSEALDGLVTQALTNNRSLA
GAAANLEQVRAGLSEARSALLPSTTATAGGQYVRDPSFAPGDVETDVYSLGFEMAYEVDIFGRVSSSVEAARQDAAAAEA
AYRGASLMVAGETARAWIDYCAGNIQLETAQSNYGLQEETLNLTRTLFDAGRGIRLDVVQAEAALRRAEADIPSVRAARD
AALFRLARLTGQTPAEMSQAVPACESMPQLVSLLPTGESATLLARRPDVRQAELQLASAAARVGIATANLYPSVSIGGSV
STASLGGAGDMFSEDGLSFGLGPLISWNFPNVAAARARLAAAEAGVDIALANFDETFLTALEEAEIALTNYAAEKERYAA
LEASAAASEEAAELARTRYRIGSDNFLNVLDAERTFIETRTALAQSEAAKASAEINVFMAMGGGWAD

Sequences:

>Translated_467_residues
MIHLKLPTLTAIAALALSACATAPAAPDSSVASRVEGALLSAEAANVAATPVPKDWWRLYQSEALDGLVTQALTNNRSLA
GAAANLEQVRAGLSEARSALLPSTTATAGGQYVRDPSFAPGDVETDVYSLGFEMAYEVDIFGRVSSSVEAARQDAAAAEA
AYRGASLMVAGETARAWIDYCAGNIQLETAQSNYGLQEETLNLTRTLFDAGRGIRLDVVQAEAALRRAEADIPSVRAARD
AALFRLARLTGQTPAEMSQAVPACESMPQLVSLLPTGESATLLARRPDVRQAELQLASAAARVGIATANLYPSVSIGGSV
STASLGGAGDMFSEDGLSFGLGPLISWNFPNVAAARARLAAAEAGVDIALANFDETFLTALEEAEIALTNYAAEKERYAA
LEASAAASEEAAELARTRYRIGSDNFLNVLDAERTFIETRTALAQSEAAKASAEINVFMAMGGGWAD
>Mature_467_residues
MIHLKLPTLTAIAALALSACATAPAAPDSSVASRVEGALLSAEAANVAATPVPKDWWRLYQSEALDGLVTQALTNNRSLA
GAAANLEQVRAGLSEARSALLPSTTATAGGQYVRDPSFAPGDVETDVYSLGFEMAYEVDIFGRVSSSVEAARQDAAAAEA
AYRGASLMVAGETARAWIDYCAGNIQLETAQSNYGLQEETLNLTRTLFDAGRGIRLDVVQAEAALRRAEADIPSVRAARD
AALFRLARLTGQTPAEMSQAVPACESMPQLVSLLPTGESATLLARRPDVRQAELQLASAAARVGIATANLYPSVSIGGSV
STASLGGAGDMFSEDGLSFGLGPLISWNFPNVAAARARLAAAEAGVDIALANFDETFLTALEEAEIALTNYAAEKERYAA
LEASAAASEEAAELARTRYRIGSDNFLNVLDAERTFIETRTALAQSEAAKASAEINVFMAMGGGWAD

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=459, Percent_Identity=25.0544662309368, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 48704; Mature: 48704

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHLKLPTLTAIAALALSACATAPAAPDSSVASRVEGALLSAEAANVAATPVPKDWWRLY
CEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
QSEALDGLVTQALTNNRSLAGAAANLEQVRAGLSEARSALLPSTTATAGGQYVRDPSFAP
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GDVETDVYSLGFEMAYEVDIFGRVSSSVEAARQDAAAAEAAYRGASLMVAGETARAWIDY
CCCCHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHH
CAGNIQLETAQSNYGLQEETLNLTRTLFDAGRGIRLDVVQAEAALRRAEADIPSVRAARD
HCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHH
AALFRLARLTGQTPAEMSQAVPACESMPQLVSLLPTGESATLLARRPDVRQAELQLASAA
HHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHH
ARVGIATANLYPSVSIGGSVSTASLGGAGDMFSEDGLSFGLGPLISWNFPNVAAARARLA
HHHEEEEECCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHH
AAEAGVDIALANFDETFLTALEEAEIALTNYAAEKERYAALEASAAASEEAAELARTRYR
HHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGSDNFLNVLDAERTFIETRTALAQSEAAKASAEINVFMAMGGGWAD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MIHLKLPTLTAIAALALSACATAPAAPDSSVASRVEGALLSAEAANVAATPVPKDWWRLY
CEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
QSEALDGLVTQALTNNRSLAGAAANLEQVRAGLSEARSALLPSTTATAGGQYVRDPSFAP
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GDVETDVYSLGFEMAYEVDIFGRVSSSVEAARQDAAAAEAAYRGASLMVAGETARAWIDY
CCCCHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHH
CAGNIQLETAQSNYGLQEETLNLTRTLFDAGRGIRLDVVQAEAALRRAEADIPSVRAARD
HCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCHHHHHH
AALFRLARLTGQTPAEMSQAVPACESMPQLVSLLPTGESATLLARRPDVRQAELQLASAA
HHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHH
ARVGIATANLYPSVSIGGSVSTASLGGAGDMFSEDGLSFGLGPLISWNFPNVAAARARLA
HHHEEEEECCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHH
AAEAGVDIALANFDETFLTALEEAEIALTNYAAEKERYAALEASAAASEEAAELARTRYR
HHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGSDNFLNVLDAERTFIETRTALAQSEAAKASAEINVFMAMGGGWAD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]