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The map label for this gene is lepA

Identifier: 114800355

GI number: 114800355

Start: 122692

End: 124506

Strand: Reverse

Name: lepA

Synonym: HNE_0139

Alternate gene names: 114800355

Gene position: 124506-122692 (Counterclockwise)

Preceding gene: 114799614

Following gene: 114798778

Centisome position: 3.36

GC content: 61.43

Gene sequence:

>1815_bases
ATGCGCCCGATGACACCGCGCGATAAAATCCGTAATTTTTCGATCATTGCCCATATCGACCATGGGAAATCGACCCTAGC
CGACCGCCTGATTCAGGCCTGCGGCGGCCTCACTGACCGTGAAATGAGTGAGCAGGTGCTCGACTCGATGGATATCGAGA
AAGAGCGCGGCATCACCATCAAGGCCCAGACCGTGCGCCTGTCCTATACGGCCAGCGATGGGGAAACCTATACGCTGAAC
CTCATGGACACCCCCGGACACGTCGACTTTGCCTATGAAGTCTCCCGGTGTCTGGCCGCCTGCGAAGGCTCTCTGCTGGT
GGTGGACGCCTCGCAAGGCGTGGAAGCCCAGACGCTCGCCAACGTCTACCAGGCCATCGACCAGAACCATGAGATCGTGC
CGGTCCTCAACAAGATCGACCTGCCTGCCGCCGATGTGGACCGGGTTAAGGAGCAGATCGAGGAAATCATCGGCCTTGAC
GCCTCTGACGCCGTGCAGATTTCGGCCAAGACGGGCCTTGGCATTCCCGACGTGCTCGAAGCCATCGTCAACCGCCTGCC
CCCGCCGCGCGGCGGCGACCACACCGCGCCGCTGAAAGCCGCCCTCGTGGACGCCTATTACGACCCCTATCTCGGCGTCG
TCGTCATCGTGCGCGTGCATGAAGGCATCCTGAAGAAGAAACAGACGATCCGCATGATGCGCACCGGCGGCGTCTACGAG
ATCGACAAGGTCGGCACGTTCAATCCCAAGCTCACCGAAGCCGATGCTCTCGGCCCCGGCGAGGTCGGCTATTTCGTCGC
CTCGATCAAGGAAGTCGGCGATTGCTCGATCGGTGACACGATCACCGAAGACAAACGCCCGACCGACAAAGCCCTGCCCG
GCTATAAAGATGTGCAGCCGGTCGTCTTCTGCGGTCTCTTCCCGATGGATGCAGGCGACTTTGACGACCTGCGCGCCGCG
ATGGGCAAGCTGCGCCTCAATGATGCGTCCTTCACCTGGGAGATGGAAACCTCCGCCGCGCTCGGCATGGGCTTCCGCTG
CGGCTTCCTCGGCCTGCTCCACCTCGAAATCATCCAGGAGCGCCTCTCCCGCGAGTTTGACCTCGACCTCATCGCGACCG
CGCCCAGTGTTGTCTATGAAATGTCGATGACCGACGGCACCGAGATCGAGCTGCACAACCCGGCAGACATGCCCGACGTT
GTCCGCATCACCGAAATCCGTGAGCCGTGGATTGCCGCGACGATCTACACGCCCGACGAATATCTCGGCTCCATCCTGAA
ACTCTGCCAGGATCGCCGCGGTATCCAGATCGAGCTATCCTACGTAGGCGGACGGGCCTGCGTGAAATACGAACTGCCCC
TCAACGAGGTGGTGTTCGACTTTTACGACCGCCTGAAATCCATCTCGCGCGGCTATGCGAGCTTTGACTATACCCTCATC
GGCCACCGCGCCGAAAACCTCGTCAAGCTGCAGATCCTCGTCAATGAGGAGCCTGTCGACGCGCTCGCCATGCTCGTCCA
CCGCGACCGCGCCGAAAGCCGGGGCCGTCAGATGTGCGAGCGCCTCAAAGAGCTGATCCCACGCCAGATGTTCAAAATCC
CGATCCAGGCGGCCATCGGCGGCCGGGTCGTCGCGCGCGAAACCATCAGTGCCATGAGGAAAGACGTGACCGCCAAATGC
TATGGCGGCGACGCCACGCGCAAACGCAAACTCCTCGACAAACAGGCCGCCGGCAAGAAACGCATGCGCCAGTTCGGCAA
GGTGGAAATCCCGCAGGAAGCGTTTGTGGCCGCGCTCAGGATGGATGGGGATTGA

Upstream 100 bases:

>100_bases
GGGGAAAACGAAGGCTAACTGACGTTTATGGCACTCGCAAGGCGAAGACCCACATCCCGCCCGCCTGCGCCCACTTTCCC
AGCGCGCAAGTGCCCGCTAT

Downstream 100 bases:

>100_bases
TCCCCATCCAACGCCCGCCTCAGCTCACCAGCAGATAAATCGCCCGCCCCGCCAGCGGGAACAGCATCAGCGTGGACACC
ACGAAAAGGTAAAACCGAAT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MRPMTPRDKIRNFSIIAHIDHGKSTLADRLIQACGGLTDREMSEQVLDSMDIEKERGITIKAQTVRLSYTASDGETYTLN
LMDTPGHVDFAYEVSRCLAACEGSLLVVDASQGVEAQTLANVYQAIDQNHEIVPVLNKIDLPAADVDRVKEQIEEIIGLD
ASDAVQISAKTGLGIPDVLEAIVNRLPPPRGGDHTAPLKAALVDAYYDPYLGVVVIVRVHEGILKKKQTIRMMRTGGVYE
IDKVGTFNPKLTEADALGPGEVGYFVASIKEVGDCSIGDTITEDKRPTDKALPGYKDVQPVVFCGLFPMDAGDFDDLRAA
MGKLRLNDASFTWEMETSAALGMGFRCGFLGLLHLEIIQERLSREFDLDLIATAPSVVYEMSMTDGTEIELHNPADMPDV
VRITEIREPWIAATIYTPDEYLGSILKLCQDRRGIQIELSYVGGRACVKYELPLNEVVFDFYDRLKSISRGYASFDYTLI
GHRAENLVKLQILVNEEPVDALAMLVHRDRAESRGRQMCERLKELIPRQMFKIPIQAAIGGRVVARETISAMRKDVTAKC
YGGDATRKRKLLDKQAAGKKRMRQFGKVEIPQEAFVAALRMDGD

Sequences:

>Translated_604_residues
MRPMTPRDKIRNFSIIAHIDHGKSTLADRLIQACGGLTDREMSEQVLDSMDIEKERGITIKAQTVRLSYTASDGETYTLN
LMDTPGHVDFAYEVSRCLAACEGSLLVVDASQGVEAQTLANVYQAIDQNHEIVPVLNKIDLPAADVDRVKEQIEEIIGLD
ASDAVQISAKTGLGIPDVLEAIVNRLPPPRGGDHTAPLKAALVDAYYDPYLGVVVIVRVHEGILKKKQTIRMMRTGGVYE
IDKVGTFNPKLTEADALGPGEVGYFVASIKEVGDCSIGDTITEDKRPTDKALPGYKDVQPVVFCGLFPMDAGDFDDLRAA
MGKLRLNDASFTWEMETSAALGMGFRCGFLGLLHLEIIQERLSREFDLDLIATAPSVVYEMSMTDGTEIELHNPADMPDV
VRITEIREPWIAATIYTPDEYLGSILKLCQDRRGIQIELSYVGGRACVKYELPLNEVVFDFYDRLKSISRGYASFDYTLI
GHRAENLVKLQILVNEEPVDALAMLVHRDRAESRGRQMCERLKELIPRQMFKIPIQAAIGGRVVARETISAMRKDVTAKC
YGGDATRKRKLLDKQAAGKKRMRQFGKVEIPQEAFVAALRMDGD
>Mature_604_residues
MRPMTPRDKIRNFSIIAHIDHGKSTLADRLIQACGGLTDREMSEQVLDSMDIEKERGITIKAQTVRLSYTASDGETYTLN
LMDTPGHVDFAYEVSRCLAACEGSLLVVDASQGVEAQTLANVYQAIDQNHEIVPVLNKIDLPAADVDRVKEQIEEIIGLD
ASDAVQISAKTGLGIPDVLEAIVNRLPPPRGGDHTAPLKAALVDAYYDPYLGVVVIVRVHEGILKKKQTIRMMRTGGVYE
IDKVGTFNPKLTEADALGPGEVGYFVASIKEVGDCSIGDTITEDKRPTDKALPGYKDVQPVVFCGLFPMDAGDFDDLRAA
MGKLRLNDASFTWEMETSAALGMGFRCGFLGLLHLEIIQERLSREFDLDLIATAPSVVYEMSMTDGTEIELHNPADMPDV
VRITEIREPWIAATIYTPDEYLGSILKLCQDRRGIQIELSYVGGRACVKYELPLNEVVFDFYDRLKSISRGYASFDYTLI
GHRAENLVKLQILVNEEPVDALAMLVHRDRAESRGRQMCERLKELIPRQMFKIPIQAAIGGRVVARETISAMRKDVTAKC
YGGDATRKRKLLDKQAAGKKRMRQFGKVEIPQEAFVAALRMDGD

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=604, Percent_Identity=47.3509933774834, Blast_Score=604, Evalue=1e-173,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=46.6165413533835, Blast_Score=115, Evalue=2e-25,
Organism=Homo sapiens, GI25306283, Length=153, Percent_Identity=44.4444444444444, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI19923640, Length=153, Percent_Identity=44.4444444444444, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI25306287, Length=153, Percent_Identity=44.4444444444444, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=40.2684563758389, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI18390331, Length=165, Percent_Identity=35.1515151515151, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=42.7272727272727, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=42.7272727272727, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=42.7272727272727, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI217272894, Length=161, Percent_Identity=33.5403726708075, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI217272892, Length=161, Percent_Identity=33.5403726708075, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI94966752, Length=97, Percent_Identity=41.2371134020619, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI53729339, Length=230, Percent_Identity=27.3913043478261, Blast_Score=68, Evalue=3e-11,
Organism=Homo sapiens, GI53729337, Length=230, Percent_Identity=27.3913043478261, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=57.8859060402685, Blast_Score=701, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=507, Percent_Identity=29.3885601577909, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1789738, Length=167, Percent_Identity=34.7305389221557, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1790835, Length=153, Percent_Identity=32.0261437908497, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1789559, Length=232, Percent_Identity=31.0344827586207, Blast_Score=76, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=617, Percent_Identity=39.708265802269, Blast_Score=465, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17556745, Length=531, Percent_Identity=25.4237288135593, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71988811, Length=136, Percent_Identity=37.5, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=136, Percent_Identity=37.5, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=36.0248447204969, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=147, Percent_Identity=34.6938775510204, Blast_Score=89, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17552882, Length=171, Percent_Identity=30.9941520467836, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI32566303, Length=305, Percent_Identity=24.9180327868852, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=604, Percent_Identity=44.3708609271523, Blast_Score=523, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6323098, Length=202, Percent_Identity=36.1386138613861, Blast_Score=110, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=157, Percent_Identity=38.8535031847134, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=157, Percent_Identity=38.8535031847134, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=117, Percent_Identity=38.4615384615385, Blast_Score=94, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=170, Percent_Identity=32.9411764705882, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI78706572, Length=604, Percent_Identity=43.3774834437086, Blast_Score=514, Evalue=1e-146,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=41.726618705036, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24582462, Length=165, Percent_Identity=35.1515151515151, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=35.2941176470588, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=35.2941176470588, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=35.2941176470588, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357743, Length=160, Percent_Identity=34.375, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI221458488, Length=143, Percent_Identity=39.1608391608392, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281363316, Length=286, Percent_Identity=29.3706293706294, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI17864358, Length=286, Percent_Identity=29.3706293706294, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19921738, Length=235, Percent_Identity=28.0851063829787, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28572034, Length=236, Percent_Identity=28.8135593220339, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_HYPNA (Q0C5X0)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_758873.1
- ProteinModelPortal:   Q0C5X0
- SMR:   Q0C5X0
- STRING:   Q0C5X0
- GeneID:   4288718
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0139
- NMPDR:   fig|228405.5.peg.137
- TIGR:   HNE_0139
- eggNOG:   COG0481
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- PhylomeDB:   Q0C5X0
- ProtClustDB:   PRK05433
- BioCyc:   HNEP81032:HNE_0139-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 66963; Mature: 66963

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPMTPRDKIRNFSIIAHIDHGKSTLADRLIQACGGLTDREMSEQVLDSMDIEKERGITI
CCCCCCHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHCCCEE
KAQTVRLSYTASDGETYTLNLMDTPGHVDFAYEVSRCLAACEGSLLVVDASQGVEAQTLA
EEEEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
NVYQAIDQNHEIVPVLNKIDLPAADVDRVKEQIEEIIGLDASDAVQISAKTGLGIPDVLE
HHHHHHCCCCCEEEEHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHH
AIVNRLPPPRGGDHTAPLKAALVDAYYDPYLGVVVIVRVHEGILKKKQTIRMMRTGGVYE
HHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
IDKVGTFNPKLTEADALGPGEVGYFVASIKEVGDCSIGDTITEDKRPTDKALPGYKDVQP
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VVFCGLFPMDAGDFDDLRAAMGKLRLNDASFTWEMETSAALGMGFRCGFLGLLHLEIIQE
EEEEEECCCCCCCHHHHHHHHHHEEECCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHH
RLSREFDLDLIATAPSVVYEMSMTDGTEIELHNPADMPDVVRITEIREPWIAATIYTPDE
HHCCCCCCEEEECCCCCEEEEECCCCCEEEECCCCCCCCCEEHHHCCCCEEEEEEECCHH
YLGSILKLCQDRRGIQIELSYVGGRACVKYELPLNEVVFDFYDRLKSISRGYASFDYTLI
HHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEE
GHRAENLVKLQILVNEEPVDALAMLVHRDRAESRGRQMCERLKELIPRQMFKIPIQAAIG
CCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
GRVVARETISAMRKDVTAKCYGGDATRKRKLLDKQAAGKKRMRQFGKVEIPQEAFVAALR
CCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHC
MDGD
CCCC
>Mature Secondary Structure
MRPMTPRDKIRNFSIIAHIDHGKSTLADRLIQACGGLTDREMSEQVLDSMDIEKERGITI
CCCCCCHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHCCCEE
KAQTVRLSYTASDGETYTLNLMDTPGHVDFAYEVSRCLAACEGSLLVVDASQGVEAQTLA
EEEEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
NVYQAIDQNHEIVPVLNKIDLPAADVDRVKEQIEEIIGLDASDAVQISAKTGLGIPDVLE
HHHHHHCCCCCEEEEHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHH
AIVNRLPPPRGGDHTAPLKAALVDAYYDPYLGVVVIVRVHEGILKKKQTIRMMRTGGVYE
HHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
IDKVGTFNPKLTEADALGPGEVGYFVASIKEVGDCSIGDTITEDKRPTDKALPGYKDVQP
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VVFCGLFPMDAGDFDDLRAAMGKLRLNDASFTWEMETSAALGMGFRCGFLGLLHLEIIQE
EEEEEECCCCCCCHHHHHHHHHHEEECCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHH
RLSREFDLDLIATAPSVVYEMSMTDGTEIELHNPADMPDVVRITEIREPWIAATIYTPDE
HHCCCCCCEEEECCCCCEEEEECCCCCEEEECCCCCCCCCEEHHHCCCCEEEEEEECCHH
YLGSILKLCQDRRGIQIELSYVGGRACVKYELPLNEVVFDFYDRLKSISRGYASFDYTLI
HHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEE
GHRAENLVKLQILVNEEPVDALAMLVHRDRAESRGRQMCERLKELIPRQMFKIPIQAAIG
CCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
GRVVARETISAMRKDVTAKCYGGDATRKRKLLDKQAAGKKRMRQFGKVEIPQEAFVAALR
CCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHC
MDGD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA