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The map label for this gene is mcl2 [H]

Identifier: 114800301

GI number: 114800301

Start: 1103731

End: 1104555

Strand: Direct

Name: mcl2 [H]

Synonym: HNE_1097

Alternate gene names: 114800301

Gene position: 1103731-1104555 (Clockwise)

Preceding gene: 114797744

Following gene: 114799274

Centisome position: 29.79

GC content: 63.88

Gene sequence:

>825_bases
ATGCCTTCAACCTATCGCTCATTCCTGTTTGTTCCCGGCGCCCGGCAGGACCGGTTTGCCAAGGCGCTGGCTGCTGGGGC
CGACGCTGCCATTATCGACCTTGAGGATGCCGTGCTGCCGTCTGAAAAGGACAAGGCCCGCGCCGATGTTCTGGGCTGGA
TAGATGCCTGGAGCGGCAAGGGGCTGGCCGTGCGGATCAACTCGCCCCGCAGCGCGCATGGATGCGCCGATATTGCCGCG
TTCGCGGCCTCGGGCGCGCTGGCGAAGCTTGATTTTATCATGGTGCCCAAGGCGGAAACGGCGGTGGATCTTGAAATCGT
CGCCGAAGCGCTTGGCGCGGATATTCCGCTGATCGCCGTTATGGAAAGCGGCCGGTCGCTCGCAAATGTACAGTCCGTGG
CCGCCAGGGCGACGGGCGGCATGCTGTTCGGCGGGGCAGACTTCTCCGCAAGCCTGGGCGCAGACCTTGCCAATTGGGAC
GCGATGCTGACTGCGCGCGGATTGGTTGTGGCAGCCTGCGGCGCAGCCGGCGTGCCTGCCTATGACGTGCCATTCCTGGA
TGTGAAAGATCCCGCCGGCCTTGCCGAGACGACGCACAAGGCGCGCCTGATGGGCTTCTCCGGGCGTGCGTGCATCCACC
CCGACCAGGTTTCGGTCGTCAACGCGGCCTTCACGCCAAGTGAGGCCGACATCGCTGAAGCGCAGGCAATTATTGATGCG
CTGAACAATGCTTCGGGCGGCGCAGTGCTGCACAAGGGCAAACTCGTTGACCGCCCCGTCATTCTCGCGGCTGAGCGCGT
GCTCGCCAAAGCCAAATCAGTCTAA

Upstream 100 bases:

>100_bases
GCTTCAGAATAGCGCAGCGGCGTAATTTCCCTGGCCGTAGTCATTGACAGCGGCTTGGTCTGGTTTACTTTGTCCGGACA
AAGTTAGTGCGGATTGTCTT

Downstream 100 bases:

>100_bases
GGAAGAAACAGACATCATGGTACAGAACTGGAAACAGGTTGGCCCGAACCGCTATCGCGAAACCTTCGGCCGCTATTTCG
AAGACTTCGAAACCGGCGAC

Product: HpcH/HpaI aldolase/citrate lyase family protein

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MPSTYRSFLFVPGARQDRFAKALAAGADAAIIDLEDAVLPSEKDKARADVLGWIDAWSGKGLAVRINSPRSAHGCADIAA
FAASGALAKLDFIMVPKAETAVDLEIVAEALGADIPLIAVMESGRSLANVQSVAARATGGMLFGGADFSASLGADLANWD
AMLTARGLVVAACGAAGVPAYDVPFLDVKDPAGLAETTHKARLMGFSGRACIHPDQVSVVNAAFTPSEADIAEAQAIIDA
LNNASGGAVLHKGKLVDRPVILAAERVLAKAKSV

Sequences:

>Translated_274_residues
MPSTYRSFLFVPGARQDRFAKALAAGADAAIIDLEDAVLPSEKDKARADVLGWIDAWSGKGLAVRINSPRSAHGCADIAA
FAASGALAKLDFIMVPKAETAVDLEIVAEALGADIPLIAVMESGRSLANVQSVAARATGGMLFGGADFSASLGADLANWD
AMLTARGLVVAACGAAGVPAYDVPFLDVKDPAGLAETTHKARLMGFSGRACIHPDQVSVVNAAFTPSEADIAEAQAIIDA
LNNASGGAVLHKGKLVDRPVILAAERVLAKAKSV
>Mature_273_residues
PSTYRSFLFVPGARQDRFAKALAAGADAAIIDLEDAVLPSEKDKARADVLGWIDAWSGKGLAVRINSPRSAHGCADIAAF
AASGALAKLDFIMVPKAETAVDLEIVAEALGADIPLIAVMESGRSLANVQSVAARATGGMLFGGADFSASLGADLANWDA
MLTARGLVVAACGAAGVPAYDVPFLDVKDPAGLAETTHKARLMGFSGRACIHPDQVSVVNAAFTPSEADIAEAQAIIDAL
NNASGGAVLHKGKLVDRPVILAAERVLAKAKSV

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=294, Percent_Identity=27.891156462585, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI87081764, Length=287, Percent_Identity=29.616724738676, Blast_Score=97, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17557458, Length=299, Percent_Identity=28.4280936454849, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 28046; Mature: 27915

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTYRSFLFVPGARQDRFAKALAAGADAAIIDLEDAVLPSEKDKARADVLGWIDAWSGK
CCCCCCCEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCC
GLAVRINSPRSAHGCADIAAFAASGALAKLDFIMVPKAETAVDLEIVAEALGADIPLIAV
EEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEE
MESGRSLANVQSVAARATGGMLFGGADFSASLGADLANWDAMLTARGLVVAACGAAGVPA
ECCCCHHHHHHHHHHHHCCCEEEECCCCCHHHCCCHHHHHHHHHHCCEEEEECCCCCCCC
YDVPFLDVKDPAGLAETTHKARLMGFSGRACIHPDQVSVVNAAFTPSEADIAEAQAIIDA
CCCCEEECCCCCCHHHHHHHHHEECCCCCCEECCCCEEEEEEECCCCHHHHHHHHHHHHH
LNNASGGAVLHKGKLVDRPVILAAERVLAKAKSV
HCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSTYRSFLFVPGARQDRFAKALAAGADAAIIDLEDAVLPSEKDKARADVLGWIDAWSGK
CCCCCCEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCC
GLAVRINSPRSAHGCADIAAFAASGALAKLDFIMVPKAETAVDLEIVAEALGADIPLIAV
EEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEE
MESGRSLANVQSVAARATGGMLFGGADFSASLGADLANWDAMLTARGLVVAACGAAGVPA
ECCCCHHHHHHHHHHHHCCCEEEECCCCCHHHCCCHHHHHHHHHHCCEEEEECCCCCCCC
YDVPFLDVKDPAGLAETTHKARLMGFSGRACIHPDQVSVVNAAFTPSEADIAEAQAIIDA
CCCCEEECCCCCCHHHHHHHHHEECCCCCCEECCCCEEEEEEECCCCHHHHHHHHHHHHH
LNNASGGAVLHKGKLVDRPVILAAERVLAKAKSV
HCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0565 {oxaoacetate}} 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA