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The map label for this gene is czcB [H]

Identifier: 114800179

GI number: 114800179

Start: 1758795

End: 1760099

Strand: Reverse

Name: czcB [H]

Synonym: HNE_1715

Alternate gene names: 114800179

Gene position: 1760099-1758795 (Counterclockwise)

Preceding gene: 114798625

Following gene: 114799378

Centisome position: 47.51

GC content: 62.84

Gene sequence:

>1305_bases
ATGATCGACCCCAAACTGACTGGGCTGGCACTGGTGCTGGCCATGACGCTCGCCGGCTGCGGCGGTTCCGGTGACACGGC
CCGCGCAGATGCTGCAGCGGTCGCCGGAGCGACAACCGACGAGCACGCGGATCATGACGATCACGCCGAAGAAGAAGACG
GGCATTCTGAAGAGGAAGGCCATGCCGACGGCGAAGAAGAAGGTCATGACGATCATGACGATCATGGCGACGAAGCGAAC
GGTGACGTTGTTCTGCTCGACAAGGCTGCCGCAGCTGAAGCCGGGATCGCCCTGTCACCGGCGGAGACCGGACCGCTCAG
TGAAACGCTCTCTTTGCCAGCAGAGCTTCGTTTTGATGCCGACCGGGTGGCGCAGGTTGCTGCGCCCGTCAGCGGCCTGG
TCTCGAGACTGGACAAGGGCGAAGGCGACAAGGTCAAGCGCGGCGAACGCCTTGCTGTCTTGTCCAGCCGCGAACTCGCC
GGTCTGAAGGCGGACTTCCTGACAGCGAAATCCGCAGAAAGCCTTGCGGCCTCGCAGTTTGCCCGTGAAGAGCGTCTGTT
TGCCGACAAGATTACGTCGCAGGCTGAACTGGATGCCGCCCAAGCCGCCCTGATCAGCGCCCGCGCCGAACGGGAAGCGA
TGGAGAACAAGCTCCACGCCGTCGGAATCAGCGATAGCGTGCTTGAGCGGCTCGGCCAAGCACCGGATGGCTCCCTGGCC
AACGCAGCCCTCATTGCCCCTCTCGGCGGCACGGTCATTTCCCGGAGTGTTTCGATTGGTTCAAGCGTTGAAGCAGGTGG
CGAGGCGCTGTTCATCATTGTGGATGACAGCCAACTCTGGGCAGACATCGCGGTCTACAAGGATGATGCCAGCGCAATTG
AAACTGGCCAGCCAGTCATCCTGCGAACGCGGGATGGCGCGGAAGCCGCCCGCGGCAAGATTGCGCTGATACTCCCCGTT
ATCAGCGAAACGACCCGCACCAGCACGGCGCGCGTGATCGTAGACAACCCGGAAAGCCGCCTCCGCCCGGGCCAGTTCGT
TACCGCCGAAATCTCGGTCGGCGAATCCGGCCGCACGCTGCATGTTCCGTCCGATGCGATCGTGGTTGTCGAGGACAAAC
CGTCAGTTTTTGTCCCCGTCGAAGGTGGATTTGCGCCGCGACCAGTAAAGCCGGGCCGCGCCAGCGCGGGTCTCACAGAA
ATCCTCGACGGGCTGGAGCAAGGCGAGCGCTTTGTCTCGGCCGGTGCCTTCACCCTGAAAGCGCAGCTTGAAAAAGACGC
TTTCGGCGACGGCCACGCCCACTAA

Upstream 100 bases:

>100_bases
ACGTGCGCCAGGCCACCATTGCCGCCCGCCGGGATGCGCGCGCGGCAGACCTTTTGCTCCGCAGCCTGATCGGCGCGGCC
CCCTTTGACGGAGACACACA

Downstream 100 bases:

>100_bases
GGACACCCCACCCCATGCTGAACCTCATGCACAGAATTGCAGGCGGACGCCTGCTGGCAGCGATTGCTGTCATCGCAATG
ACCCTGGGCGGACTTTTTGC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MIDPKLTGLALVLAMTLAGCGGSGDTARADAAAVAGATTDEHADHDDHAEEEDGHSEEEGHADGEEEGHDDHDDHGDEAN
GDVVLLDKAAAAEAGIALSPAETGPLSETLSLPAELRFDADRVAQVAAPVSGLVSRLDKGEGDKVKRGERLAVLSSRELA
GLKADFLTAKSAESLAASQFAREERLFADKITSQAELDAAQAALISARAEREAMENKLHAVGISDSVLERLGQAPDGSLA
NAALIAPLGGTVISRSVSIGSSVEAGGEALFIIVDDSQLWADIAVYKDDASAIETGQPVILRTRDGAEAARGKIALILPV
ISETTRTSTARVIVDNPESRLRPGQFVTAEISVGESGRTLHVPSDAIVVVEDKPSVFVPVEGGFAPRPVKPGRASAGLTE
ILDGLEQGERFVSAGAFTLKAQLEKDAFGDGHAH

Sequences:

>Translated_434_residues
MIDPKLTGLALVLAMTLAGCGGSGDTARADAAAVAGATTDEHADHDDHAEEEDGHSEEEGHADGEEEGHDDHDDHGDEAN
GDVVLLDKAAAAEAGIALSPAETGPLSETLSLPAELRFDADRVAQVAAPVSGLVSRLDKGEGDKVKRGERLAVLSSRELA
GLKADFLTAKSAESLAASQFAREERLFADKITSQAELDAAQAALISARAEREAMENKLHAVGISDSVLERLGQAPDGSLA
NAALIAPLGGTVISRSVSIGSSVEAGGEALFIIVDDSQLWADIAVYKDDASAIETGQPVILRTRDGAEAARGKIALILPV
ISETTRTSTARVIVDNPESRLRPGQFVTAEISVGESGRTLHVPSDAIVVVEDKPSVFVPVEGGFAPRPVKPGRASAGLTE
ILDGLEQGERFVSAGAFTLKAQLEKDAFGDGHAH
>Mature_434_residues
MIDPKLTGLALVLAMTLAGCGGSGDTARADAAAVAGATTDEHADHDDHAEEEDGHSEEEGHADGEEEGHDDHDDHGDEAN
GDVVLLDKAAAAEAGIALSPAETGPLSETLSLPAELRFDADRVAQVAAPVSGLVSRLDKGEGDKVKRGERLAVLSSRELA
GLKADFLTAKSAESLAASQFAREERLFADKITSQAELDAAQAALISARAEREAMENKLHAVGISDSVLERLGQAPDGSLA
NAALIAPLGGTVISRSVSIGSSVEAGGEALFIIVDDSQLWADIAVYKDDASAIETGQPVILRTRDGAEAARGKIALILPV
ISETTRTSTARVIVDNPESRLRPGQFVTAEISVGESGRTLHVPSDAIVVVEDKPSVFVPVEGGFAPRPVKPGRASAGLTE
ILDGLEQGERFVSAGAFTLKAQLEKDAFGDGHAH

Specific function: CzcA and CzcB together would act in zinc efflux nearly as effectively as the complete czc efflux system (CzcABC). The CzcB protein is thought to funnel zinc cations to the CzcA transport protein [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Attached To The Inner Membrane By A Lipid Anchor [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=298, Percent_Identity=27.1812080536913, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005695
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44954; Mature: 44954

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDPKLTGLALVLAMTLAGCGGSGDTARADAAAVAGATTDEHADHDDHAEEEDGHSEEEG
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
HADGEEEGHDDHDDHGDEANGDVVLLDKAAAAEAGIALSPAETGPLSETLSLPAELRFDA
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEECCCCCCCCHHHHCCCHHCCCCH
DRVAQVAAPVSGLVSRLDKGEGDKVKRGERLAVLSSRELAGLKADFLTAKSAESLAASQF
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCHHHHHHHHH
AREERLFADKITSQAELDAAQAALISARAEREAMENKLHAVGISDSVLERLGQAPDGSLA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHCCCCCCCCC
NAALIAPLGGTVISRSVSIGSSVEAGGEALFIIVDDSQLWADIAVYKDDASAIETGQPVI
CEEEEECCCCHHHHCEECCCCCCCCCCCEEEEEEECCCEEEEEEEEECCCCHHCCCCCEE
LRTRDGAEAARGKIALILPVISETTRTSTARVIVDNPESRLRPGQFVTAEISVGESGRTL
EEECCCCHHHCCCEEEEEEECCCCCCCCEEEEEEECCHHHCCCCCEEEEEEEECCCCCEE
HVPSDAIVVVEDKPSVFVPVEGGFAPRPVKPGRASAGLTEILDGLEQGERFVSAGAFTLK
ECCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEE
AQLEKDAFGDGHAH
EECCCCCCCCCCCC
>Mature Secondary Structure
MIDPKLTGLALVLAMTLAGCGGSGDTARADAAAVAGATTDEHADHDDHAEEEDGHSEEEG
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
HADGEEEGHDDHDDHGDEANGDVVLLDKAAAAEAGIALSPAETGPLSETLSLPAELRFDA
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEECCCCCCCCHHHHCCCHHCCCCH
DRVAQVAAPVSGLVSRLDKGEGDKVKRGERLAVLSSRELAGLKADFLTAKSAESLAASQF
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCHHHHHHHHH
AREERLFADKITSQAELDAAQAALISARAEREAMENKLHAVGISDSVLERLGQAPDGSLA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHCCCCCCCCC
NAALIAPLGGTVISRSVSIGSSVEAGGEALFIIVDDSQLWADIAVYKDDASAIETGQPVI
CEEEEECCCCHHHHCEECCCCCCCCCCCEEEEEEECCCEEEEEEEEECCCCHHCCCCCEE
LRTRDGAEAARGKIALILPVISETTRTSTARVIVDNPESRLRPGQFVTAEISVGESGRTL
EEECCCCHHHCCCEEEEEEECCCCCCCCEEEEEEECCHHHCCCCCEEEEEEEECCCCCEE
HVPSDAIVVVEDKPSVFVPVEGGFAPRPVKPGRASAGLTEILDGLEQGERFVSAGAFTLK
ECCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEE
AQLEKDAFGDGHAH
EECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8829543 [H]