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The map label for this gene is gatA [H]

Identifier: 114800109

GI number: 114800109

Start: 1241805

End: 1243325

Strand: Direct

Name: gatA [H]

Synonym: HNE_1220

Alternate gene names: 114800109

Gene position: 1241805-1243325 (Clockwise)

Preceding gene: 114800544

Following gene: 114799164

Centisome position: 33.52

GC content: 67.06

Gene sequence:

>1521_bases
GTGCCCACACCGCCAAATTACGAAGCCGATGCCACGCTGGCACAAACCCTGCCTGAGCTCTCCGCCGCCCTCGCCAGCGG
CGCGCTGACCTCCCGCGATCTCACCGCCGCCTATCTCGCCCGCATCGAGGCGGTCGACCGCGCCGGCCCGCGCCTGCAAT
CGATCATCGCGCTCAACCCCAACGCCCTCACCCAGGCCGAAGCGTCAGACGCCCGCCGCGCAGCCGGCCAGGCCCTCGGC
CCCCTGGATGGCATCCCCATCCTCCTCAAGGACAATATCGAAACCCTCGACCCGGTGCCCACCACGGCGGGCTCCCTCGC
CCTGAAAGACAACCTCACCGGCCGCGACAGCCCCCTCGCCGCCGGCCTGCGCGCCCAGGGCGCCGTCATCCTCGGCAAGA
CCAATCTCAGCCAGTGGGCAAACTTCCGCTCCAACAATTCCATCAGCGGCTGGTCCTCCGTAGGCGGACAGGTCCGTAAC
CCCCACATGCTCGACCGCTCCCCCTGCGGCTCCAGCTCTGGCACAGGCGCGGCCATCGCCGCCTCGCTCGCGGCCGCTGG
CGTGGGCACGGAAACCAACGGCTCCATCATCTGCCCGGCCAACGTCAACGGCCTCGTCGGCTTCAAGCCGACAGTCGGGC
GCATTCCCCAGCAGGGCATCGTCCCCATCTCACCCAGCCAGGACACCGCCGGCCCCATGACGAAAACCGTCACCGGCGCC
GCCCTCCTGATGGACGCCATGGACGCGGGCGAGACCGGTTACGCCGCCGCCCTCTCCACCGAAGCGCTGAAGGGAAAACG
CATCGGCGTCCTGCGCGCCGTCGTCGGCTCCAATCAAGACATCATCACCCGCTTCAACGGATCCGTCAGTATCCTGCGCA
GTCTGGGCGCTGAAATCGTCGAGATCGAGAAGTACAGCGTTCCCGGTGATTTCTGGGCCGCCACGCTGCTGGTGCTGCAA
TACGAGTTCAAAGACAGCCTCAACACCTATCTCGCCGCCGCGCCGCCAGCCGTCAAAACCCGCACGCTGGCCGATGTGAT
CGCCTTCAACATCGAACATGCAGACACCGAACTGTCCCTGTTCAATCAGGACCTGTTCGAGGCGTCCGATGCCCTCGGCC
CGCTCACGGATGAAGCCTACACAAACGCCCTGGCCATCGTCCAGAACGCCACCCGCGCGGAGGGCATAGACCGTCTGTTG
ACGGAATACAGCCTCGACGCAATCGTCGCGCCGTCCGGCCCGCTGGCGCCGCGGGTCGATCCCATCAATGGCGATGTCTG
GCCCGAATGGGCCGGCGCGGGCTTCCTCGCCGCCGTCTCCGGCTATCCCCATCTCACCGTCCCGATGGGCGAAGTCCACG
GCGTCCCGCTCGGTTTCTCCTTCCTGGGCGGCAGGGATACTGACGCCGCCATCCTCGCCTATGGCTACGCCTACGAGCAG
GCCACCCGGCTACGCCCCGATCCCAAATATCTGCAAACCGCCGAAGACCGCCCGGAGATCGCCGCCGCAACGTTGCGCTA
A

Upstream 100 bases:

>100_bases
GGGGCATCCCATGCTACGCCACATCACCGCCGCGCTCGCCGCGTTCACACTCGCCGCCTGCGCCACGCCGGCACCCGCCC
TTGAGGGAGCCGCGCCCGCC

Downstream 100 bases:

>100_bases
TCACGTCCCCTCCTTGCGCCTCCCGCTGGGGATGCGCAGGAACAGGCGCCCCAATCAAGCCACACCGGAACAGGGGTATT
CTGCTCCTCCCGGTGCCATG

Product: amidase family protein

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MPTPPNYEADATLAQTLPELSAALASGALTSRDLTAAYLARIEAVDRAGPRLQSIIALNPNALTQAEASDARRAAGQALG
PLDGIPILLKDNIETLDPVPTTAGSLALKDNLTGRDSPLAAGLRAQGAVILGKTNLSQWANFRSNNSISGWSSVGGQVRN
PHMLDRSPCGSSSGTGAAIAASLAAAGVGTETNGSIICPANVNGLVGFKPTVGRIPQQGIVPISPSQDTAGPMTKTVTGA
ALLMDAMDAGETGYAAALSTEALKGKRIGVLRAVVGSNQDIITRFNGSVSILRSLGAEIVEIEKYSVPGDFWAATLLVLQ
YEFKDSLNTYLAAAPPAVKTRTLADVIAFNIEHADTELSLFNQDLFEASDALGPLTDEAYTNALAIVQNATRAEGIDRLL
TEYSLDAIVAPSGPLAPRVDPINGDVWPEWAGAGFLAAVSGYPHLTVPMGEVHGVPLGFSFLGGRDTDAAILAYGYAYEQ
ATRLRPDPKYLQTAEDRPEIAAATLR

Sequences:

>Translated_506_residues
MPTPPNYEADATLAQTLPELSAALASGALTSRDLTAAYLARIEAVDRAGPRLQSIIALNPNALTQAEASDARRAAGQALG
PLDGIPILLKDNIETLDPVPTTAGSLALKDNLTGRDSPLAAGLRAQGAVILGKTNLSQWANFRSNNSISGWSSVGGQVRN
PHMLDRSPCGSSSGTGAAIAASLAAAGVGTETNGSIICPANVNGLVGFKPTVGRIPQQGIVPISPSQDTAGPMTKTVTGA
ALLMDAMDAGETGYAAALSTEALKGKRIGVLRAVVGSNQDIITRFNGSVSILRSLGAEIVEIEKYSVPGDFWAATLLVLQ
YEFKDSLNTYLAAAPPAVKTRTLADVIAFNIEHADTELSLFNQDLFEASDALGPLTDEAYTNALAIVQNATRAEGIDRLL
TEYSLDAIVAPSGPLAPRVDPINGDVWPEWAGAGFLAAVSGYPHLTVPMGEVHGVPLGFSFLGGRDTDAAILAYGYAYEQ
ATRLRPDPKYLQTAEDRPEIAAATLR
>Mature_505_residues
PTPPNYEADATLAQTLPELSAALASGALTSRDLTAAYLARIEAVDRAGPRLQSIIALNPNALTQAEASDARRAAGQALGP
LDGIPILLKDNIETLDPVPTTAGSLALKDNLTGRDSPLAAGLRAQGAVILGKTNLSQWANFRSNNSISGWSSVGGQVRNP
HMLDRSPCGSSSGTGAAIAASLAAAGVGTETNGSIICPANVNGLVGFKPTVGRIPQQGIVPISPSQDTAGPMTKTVTGAA
LLMDAMDAGETGYAAALSTEALKGKRIGVLRAVVGSNQDIITRFNGSVSILRSLGAEIVEIEKYSVPGDFWAATLLVLQY
EFKDSLNTYLAAAPPAVKTRTLADVIAFNIEHADTELSLFNQDLFEASDALGPLTDEAYTNALAIVQNATRAEGIDRLLT
EYSLDAIVAPSGPLAPRVDPINGDVWPEWAGAGFLAAVSGYPHLTVPMGEVHGVPLGFSFLGGRDTDAAILAYGYAYEQA
TRLRPDPKYLQTAEDRPEIAAATLR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=525, Percent_Identity=25.9047619047619, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI195972892, Length=490, Percent_Identity=25.7142857142857, Blast_Score=102, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17537465, Length=510, Percent_Identity=23.5294117647059, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=238, Percent_Identity=28.9915966386555, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=431, Percent_Identity=22.969837587007, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=453, Percent_Identity=25.3863134657837, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=250, Percent_Identity=30, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320448, Length=205, Percent_Identity=34.6341463414634, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648113, Length=242, Percent_Identity=32.6446280991736, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21356731, Length=255, Percent_Identity=29.4117647058824, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24644968, Length=274, Percent_Identity=27.7372262773723, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24652985, Length=259, Percent_Identity=29.3436293436293, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19922090, Length=259, Percent_Identity=29.3436293436293, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24652981, Length=259, Percent_Identity=29.3436293436293, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24652983, Length=259, Percent_Identity=29.3436293436293, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI45550774, Length=242, Percent_Identity=26.4462809917355, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648435, Length=242, Percent_Identity=26.4462809917355, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648437, Length=242, Percent_Identity=26.4462809917355, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648441, Length=176, Percent_Identity=28.4090909090909, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24648439, Length=176, Percent_Identity=28.4090909090909, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 52652; Mature: 52521

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTPPNYEADATLAQTLPELSAALASGALTSRDLTAAYLARIEAVDRAGPRLQSIIALNP
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECC
NALTQAEASDARRAAGQALGPLDGIPILLKDNIETLDPVPTTAGSLALKDNLTGRDSPLA
CHHCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCHH
AGLRAQGAVILGKTNLSQWANFRSNNSISGWSSVGGQVRNPHMLDRSPCGSSSGTGAAIA
CCCCCCCEEEEECCCHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
ASLAAAGVGTETNGSIICPANVNGLVGFKPTVGRIPQQGIVPISPSQDTAGPMTKTVTGA
HHHHHHCCCCCCCCCEEECCCCCCEEECCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHH
ALLMDAMDAGETGYAAALSTEALKGKRIGVLRAVVGSNQDIITRFNGSVSILRSLGAEIV
HHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHCCCCEE
EIEKYSVPGDFWAATLLVLQYEFKDSLNTYLAAAPPAVKTRTLADVIAFNIEHADTELSL
EEEEECCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHEECCCCCCHHHH
FNQDLFEASDALGPLTDEAYTNALAIVQNATRAEGIDRLLTEYSLDAIVAPSGPLAPRVD
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCC
PINGDVWPEWAGAGFLAAVSGYPHLTVPMGEVHGVPLGFSFLGGRDTDAAILAYGYAYEQ
CCCCCCCCCCCCCCCEEEECCCCEEEEECHHHCCCCCCCCCCCCCCCCCEEEEEHHHHHH
ATRLRPDPKYLQTAEDRPEIAAATLR
HHHCCCCHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
PTPPNYEADATLAQTLPELSAALASGALTSRDLTAAYLARIEAVDRAGPRLQSIIALNP
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECC
NALTQAEASDARRAAGQALGPLDGIPILLKDNIETLDPVPTTAGSLALKDNLTGRDSPLA
CHHCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCHH
AGLRAQGAVILGKTNLSQWANFRSNNSISGWSSVGGQVRNPHMLDRSPCGSSSGTGAAIA
CCCCCCCEEEEECCCHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
ASLAAAGVGTETNGSIICPANVNGLVGFKPTVGRIPQQGIVPISPSQDTAGPMTKTVTGA
HHHHHHCCCCCCCCCEEECCCCCCEEECCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHH
ALLMDAMDAGETGYAAALSTEALKGKRIGVLRAVVGSNQDIITRFNGSVSILRSLGAEIV
HHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHCCCCEE
EIEKYSVPGDFWAATLLVLQYEFKDSLNTYLAAAPPAVKTRTLADVIAFNIEHADTELSL
EEEEECCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHEECCCCCCHHHH
FNQDLFEASDALGPLTDEAYTNALAIVQNATRAEGIDRLLTEYSLDAIVAPSGPLAPRVD
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCC
PINGDVWPEWAGAGFLAAVSGYPHLTVPMGEVHGVPLGFSFLGGRDTDAAILAYGYAYEQ
CCCCCCCCCCCCCCCEEEECCCCEEEEECHHHCCCCCCCCCCCCCCCCCEEEEEHHHHHH
ATRLRPDPKYLQTAEDRPEIAAATLR
HHHCCCCHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA