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The map label for this gene is oprM [H]

Identifier: 114800045

GI number: 114800045

Start: 1687311

End: 1688663

Strand: Direct

Name: oprM [H]

Synonym: HNE_1648

Alternate gene names: 114800045

Gene position: 1687311-1688663 (Clockwise)

Preceding gene: 114800370

Following gene: 114798397

Centisome position: 45.54

GC content: 66.3

Gene sequence:

>1353_bases
ATGATCCGCACGCTGCTCATCACCGCATCCGTCCTCACGCTCACCGGCTGCGCCAGCCTGAACCTCATCGAGCGCGAAAC
CTCCACCGATGCCGGCCTCGCCATGCCAGATGGCTACGACTATGCCAGCCAGGTCGCCGCCCTCAGCGCCCCCGAACAGG
CCTGGTGGCAGGGCTTCAACTCCGCAGACCTCGACGCCCTCATTACAGAAGCCCTCGCCGCCAACAACACGCTCGCCCAG
GGTCTCGCCAATGTCGACGCCTCCCGCGCTTCGCTCCGCACGGCCAATGCCGCCTTCCTCCCACAAGCCAGCGGCAGCCT
CTCCAGCAGCAGCAACACCAGCGCAGGCCTCGATGATGTTGACGCCAGCGCCCGCCTTTCGGCCAGCTACCAGCTCGACC
TCTTCGGCGCCAACGCCGCCGGCCGCAACGCCGCCCTCGCAAATCTCGACGCCGCCATCTTCGCCCAGCGCGCACTGGAG
CTGACCGTTCAGTCAGATGTCGCCGCCGGCTGGTTCAATCTTCTCTCCGCCCGCGAGCAACTCGCCGTCGCCCGGCGGAA
CCTCGAAATCTCCGAACGCATCTTCGAGATTGTCCAGTTCCGTTACGAGGCCGGCGTTATCTCCGGGTTTGATGTGTCCA
GCCAGTCGGCCCAACTCGCCAATGCCCGCGCACGCATCCCGCAGCTGGAATCCCAGATCACCAGCCAGGAAACCGCCCTC
GGCATCCTCCTTGGCCGGGTGCCGCAAGGCTACACCGCTCCGCAGGCTGACATACTCTCCATCGCGCTGCCCGCCGCAGA
ACCCGGCCTGCCATCCGATCTGCTGCTGCGCCGGCCAGACCTGATGCAGTCCGAGGCCAGCCTGCGCGCAGCCAATGCCA
ATATAGATGCGGCCCGCGCCGCCTTCTTCCCCAGCATCGATCTGGGGGCAGGGCTCTCCAGCGCGTTGACAGGGGGCACC
GACCTTATCGGTTCGCTTTCGGCCTCGCTCGCCCAGACGATCTTTTCCGGTGGCCGCCTTGATGCCCAGCTCGAGGGCGC
TCAGGCCCGCCGCGAAGGCCAGCTCGCCGGTTATCGCCAATCGATCCTCAGCGCGCTGCGCGATGTCGATGTCAGCCTCA
AAGCCATCGACGCGAACGAAGTCCGCGAAGACCAGCTGCTGATCGCGCGCGACGCCGCCGAAGACGCGCTGCGCGCCGCT
GAAATCCAGTACCAGGCAGGCACGGGCGACCTCACAAGCCTCCTGAACGCCCAGCAGAACTATTTCGATGCCGCCAACAG
CTATGTGCTGGGCCGGCTCGACCGGCTGACCGCCGCCATCAATCTCTATGTGGCCGTTGGCGGCGGCTACTGA

Upstream 100 bases:

>100_bases
TCAGCTCGGCGCTCGGCACAGGCCTCGTCTTCGGCCTCCTGCCAGCCCGCCAGGCTTCGCGCCTCGACCCCGTCGCCGCG
CTCGCATCGGAGTAGACCCC

Downstream 100 bases:

>100_bases
CCATAAATCGGGCAGGCGAGGTGGTGGCGCCCGCTCAGACGCGTGCGGCACAAAATTCAATGGTGCCAAGGGGATAAACG
CCCCTGCGACACCTTGAACA

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MIRTLLITASVLTLTGCASLNLIERETSTDAGLAMPDGYDYASQVAALSAPEQAWWQGFNSADLDALITEALAANNTLAQ
GLANVDASRASLRTANAAFLPQASGSLSSSSNTSAGLDDVDASARLSASYQLDLFGANAAGRNAALANLDAAIFAQRALE
LTVQSDVAAGWFNLLSAREQLAVARRNLEISERIFEIVQFRYEAGVISGFDVSSQSAQLANARARIPQLESQITSQETAL
GILLGRVPQGYTAPQADILSIALPAAEPGLPSDLLLRRPDLMQSEASLRAANANIDAARAAFFPSIDLGAGLSSALTGGT
DLIGSLSASLAQTIFSGGRLDAQLEGAQARREGQLAGYRQSILSALRDVDVSLKAIDANEVREDQLLIARDAAEDALRAA
EIQYQAGTGDLTSLLNAQQNYFDAANSYVLGRLDRLTAAINLYVAVGGGY

Sequences:

>Translated_450_residues
MIRTLLITASVLTLTGCASLNLIERETSTDAGLAMPDGYDYASQVAALSAPEQAWWQGFNSADLDALITEALAANNTLAQ
GLANVDASRASLRTANAAFLPQASGSLSSSSNTSAGLDDVDASARLSASYQLDLFGANAAGRNAALANLDAAIFAQRALE
LTVQSDVAAGWFNLLSAREQLAVARRNLEISERIFEIVQFRYEAGVISGFDVSSQSAQLANARARIPQLESQITSQETAL
GILLGRVPQGYTAPQADILSIALPAAEPGLPSDLLLRRPDLMQSEASLRAANANIDAARAAFFPSIDLGAGLSSALTGGT
DLIGSLSASLAQTIFSGGRLDAQLEGAQARREGQLAGYRQSILSALRDVDVSLKAIDANEVREDQLLIARDAAEDALRAA
EIQYQAGTGDLTSLLNAQQNYFDAANSYVLGRLDRLTAAINLYVAVGGGY
>Mature_450_residues
MIRTLLITASVLTLTGCASLNLIERETSTDAGLAMPDGYDYASQVAALSAPEQAWWQGFNSADLDALITEALAANNTLAQ
GLANVDASRASLRTANAAFLPQASGSLSSSSNTSAGLDDVDASARLSASYQLDLFGANAAGRNAALANLDAAIFAQRALE
LTVQSDVAAGWFNLLSAREQLAVARRNLEISERIFEIVQFRYEAGVISGFDVSSQSAQLANARARIPQLESQITSQETAL
GILLGRVPQGYTAPQADILSIALPAAEPGLPSDLLLRRPDLMQSEASLRAANANIDAARAAFFPSIDLGAGLSSALTGGT
DLIGSLSASLAQTIFSGGRLDAQLEGAQARREGQLAGYRQSILSALRDVDVSLKAIDANEVREDQLLIARDAAEDALRAA
EIQYQAGTGDLTSLLNAQQNYFDAANSYVLGRLDRLTAAINLYVAVGGGY

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=466, Percent_Identity=30.4721030042918, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI2367348, Length=481, Percent_Identity=28.6902286902287, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 47130; Mature: 47130

Theoretical pI: Translated: 4.19; Mature: 4.19

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRTLLITASVLTLTGCASLNLIERETSTDAGLAMPDGYDYASQVAALSAPEQAWWQGFN
CCEEEEEHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHCCCC
SADLDALITEALAANNTLAQGLANVDASRASLRTANAAFLPQASGSLSSSSNTSAGLDDV
CCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCC
DASARLSASYQLDLFGANAAGRNAALANLDAAIFAQRALELTVQSDVAAGWFNLLSAREQ
CCCCEECCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHH
LAVARRNLEISERIFEIVQFRYEAGVISGFDVSSQSAQLANARARIPQLESQITSQETAL
HHHHHHCCHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHH
GILLGRVPQGYTAPQADILSIALPAAEPGLPSDLLLRRPDLMQSEASLRAANANIDAARA
HHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCHHHH
AFFPSIDLGAGLSSALTGGTDLIGSLSASLAQTIFSGGRLDAQLEGAQARREGQLAGYRQ
HHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHCCCHHHHHH
SILSALRDVDVSLKAIDANEVREDQLLIARDAAEDALRAAEIQYQAGTGDLTSLLNAQQN
HHHHHHHCCCCEEEEECHHHCCCCCEEEEHHHHHHHHHHHHEEEECCCCHHHHHHHHHHH
YFDAANSYVLGRLDRLTAAINLYVAVGGGY
HHHHHCHHHHHHHHHHHEEEEEEEEECCCC
>Mature Secondary Structure
MIRTLLITASVLTLTGCASLNLIERETSTDAGLAMPDGYDYASQVAALSAPEQAWWQGFN
CCEEEEEHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHCCCC
SADLDALITEALAANNTLAQGLANVDASRASLRTANAAFLPQASGSLSSSSNTSAGLDDV
CCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCC
DASARLSASYQLDLFGANAAGRNAALANLDAAIFAQRALELTVQSDVAAGWFNLLSAREQ
CCCCEECCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHH
LAVARRNLEISERIFEIVQFRYEAGVISGFDVSSQSAQLANARARIPQLESQITSQETAL
HHHHHHCCHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHH
GILLGRVPQGYTAPQADILSIALPAAEPGLPSDLLLRRPDLMQSEASLRAANANIDAARA
HHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCHHHH
AFFPSIDLGAGLSSALTGGTDLIGSLSASLAQTIFSGGRLDAQLEGAQARREGQLAGYRQ
HHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHCCCHHHHHH
SILSALRDVDVSLKAIDANEVREDQLLIARDAAEDALRAAEIQYQAGTGDLTSLLNAQQN
HHHHHHHCCCCEEEEECHHHCCCCCEEEEHHHHHHHHHHHHEEEECCCCHHHHHHHHHHH
YFDAANSYVLGRLDRLTAAINLYVAVGGGY
HHHHHCHHHHHHHHHHHEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]