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The map label for this gene is hdhA [C]

Identifier: 114800019

GI number: 114800019

Start: 2321796

End: 2322728

Strand: Reverse

Name: hdhA [C]

Synonym: HNE_2221

Alternate gene names: 114800019

Gene position: 2322728-2321796 (Counterclockwise)

Preceding gene: 114798404

Following gene: 114799506

Centisome position: 62.69

GC content: 63.13

Gene sequence:

>933_bases
ATGAGGAACAGCACGCTTCCGGAGCACTGGACGGCAGCTTACATTCCCGATCAGCGGGGTCGCTTCGCCGTCATCACCGG
AACAGGGGGGCTGGGGTTCGAAGGCGCCGTTGCGCTCGCACGGTCCGGAGCCAGTGTTGTGCTTGCAGGCCGCAATGCTC
ATAAGGGAGCGGACGCCGTCGCCGCAATCCGTCAGGCAGCGCCTGCGTCCGACATACGCTATGAACGTCTTGACCTTGCC
TGCCTTGCCTCCGTGGCAGACTTCGCCGCCCGGCTCCGCGGACAGATGGATCGCCTTGATCTGCTGATCAACAATGCTGC
CGTGATGAATCCGCCCGCGCGGCAGGAAACGGAAGACGGGTTCGAGCTTCAGCTGGGCACGAATTATCTGGGTCATTTTG
CGCTCACCGGACACCTCCTACCGCTGCTACGCAAGGGCGCGAATGCCCGCGTTATCTCTGTATCCAGCATTGCTGCCCGG
AACGGCTTGATCAATCTCGAAGATCTTCAGGCGGAGCAAAGTTACCGGCCGGGAAGCGCTTATGCGCAGTCGAAGCTCGC
CTGCCTGATGTTTGCGCTGGAGCTGCACCGGCGCAGCCAGTTGGGCGGATGGGGCGTTGCCAGCATTGCGGCGCACCCCG
GTGTTTCCCGCACCGAACTGCTGCACAATGCGCCTGGGCGGGGAAGCCCTGCCAGTCTTGCCCGCAGCCTGCTCTGGTTC
CTGTTTCAGCCGGCAGATCAGGGCGCTCTGCCGACCCTGTTTGCGGCAACCTGGCCGGAGGCAAAGTCGGGCGCCTATTA
CGGGCCTCATGGCCTGAGCGAAACACGGGGGTTCCCAGCGGCGGCGCAAATTCCCCCGCAGGCCCTGGACGCAGCCCTTT
CTGCAAGGCTTTGGGAGGTATCCGAAAACCTCACAGGTGTGACATTGCGTTGA

Upstream 100 bases:

>100_bases
GCGCAGCGGGTAGTCTGCAGGTATGACAGGCGCGGAGTCTCGCGCTATGCCCGGCAAGTGATCATCCGCTTGCAGATATC
TGGAAAAGGCAGTTTGGAAC

Downstream 100 bases:

>100_bases
AACGCAGGCCGTGATTCAGGACGCAATCTCTGATTTCCAGTGGTTCCAGGTGCGTTCAACGACAATAGGGCCACCGCCCG
AGGTGGCGGCGGCCTCTTCC

Product: short chain dehydrogenase/reductase family oxidoreductase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MRNSTLPEHWTAAYIPDQRGRFAVITGTGGLGFEGAVALARSGASVVLAGRNAHKGADAVAAIRQAAPASDIRYERLDLA
CLASVADFAARLRGQMDRLDLLINNAAVMNPPARQETEDGFELQLGTNYLGHFALTGHLLPLLRKGANARVISVSSIAAR
NGLINLEDLQAEQSYRPGSAYAQSKLACLMFALELHRRSQLGGWGVASIAAHPGVSRTELLHNAPGRGSPASLARSLLWF
LFQPADQGALPTLFAATWPEAKSGAYYGPHGLSETRGFPAAAQIPPQALDAALSARLWEVSENLTGVTLR

Sequences:

>Translated_310_residues
MRNSTLPEHWTAAYIPDQRGRFAVITGTGGLGFEGAVALARSGASVVLAGRNAHKGADAVAAIRQAAPASDIRYERLDLA
CLASVADFAARLRGQMDRLDLLINNAAVMNPPARQETEDGFELQLGTNYLGHFALTGHLLPLLRKGANARVISVSSIAAR
NGLINLEDLQAEQSYRPGSAYAQSKLACLMFALELHRRSQLGGWGVASIAAHPGVSRTELLHNAPGRGSPASLARSLLWF
LFQPADQGALPTLFAATWPEAKSGAYYGPHGLSETRGFPAAAQIPPQALDAALSARLWEVSENLTGVTLR
>Mature_310_residues
MRNSTLPEHWTAAYIPDQRGRFAVITGTGGLGFEGAVALARSGASVVLAGRNAHKGADAVAAIRQAAPASDIRYERLDLA
CLASVADFAARLRGQMDRLDLLINNAAVMNPPARQETEDGFELQLGTNYLGHFALTGHLLPLLRKGANARVISVSSIAAR
NGLINLEDLQAEQSYRPGSAYAQSKLACLMFALELHRRSQLGGWGVASIAAHPGVSRTELLHNAPGRGSPASLARSLLWF
LFQPADQGALPTLFAATWPEAKSGAYYGPHGLSETRGFPAAAQIPPQALDAALSARLWEVSENLTGVTLR

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI225579078, Length=303, Percent_Identity=38.9438943894389, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI186928839, Length=295, Percent_Identity=39.6610169491525, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI193804850, Length=299, Percent_Identity=35.1170568561873, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI10190746, Length=304, Percent_Identity=35.8552631578947, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI166795268, Length=294, Percent_Identity=35.7142857142857, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI19923931, Length=255, Percent_Identity=40.7843137254902, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI146231950, Length=298, Percent_Identity=37.9194630872483, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI7706523, Length=303, Percent_Identity=34.3234323432343, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17558006, Length=299, Percent_Identity=32.4414715719064, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17532805, Length=304, Percent_Identity=32.2368421052632, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71992402, Length=275, Percent_Identity=32.7272727272727, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI193207620, Length=310, Percent_Identity=32.258064516129, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17505416, Length=270, Percent_Identity=30.7407407407407, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17532791, Length=301, Percent_Identity=28.2392026578073, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI32565532, Length=209, Percent_Identity=33.0143540669856, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI72001364, Length=306, Percent_Identity=29.7385620915033, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI115533174, Length=292, Percent_Identity=30.4794520547945, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI115533172, Length=292, Percent_Identity=30.4794520547945, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17535059, Length=267, Percent_Identity=28.8389513108614, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324820, Length=329, Percent_Identity=29.1793313069909, Blast_Score=103, Evalue=3e-23,
Organism=Drosophila melanogaster, GI28573296, Length=298, Percent_Identity=39.9328859060403, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI22024069, Length=296, Percent_Identity=38.1756756756757, Blast_Score=161, Evalue=5e-40,
Organism=Drosophila melanogaster, GI19921754, Length=296, Percent_Identity=39.1891891891892, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24586328, Length=298, Percent_Identity=36.5771812080537, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI161076371, Length=256, Percent_Identity=38.671875, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI45554248, Length=253, Percent_Identity=39.5256916996047, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24640117, Length=253, Percent_Identity=39.5256916996047, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24656154, Length=257, Percent_Identity=34.6303501945525, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922628, Length=257, Percent_Identity=34.6303501945525, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24647946, Length=295, Percent_Identity=30.8474576271186, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45553409, Length=295, Percent_Identity=30.8474576271186, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24647948, Length=295, Percent_Identity=30.8474576271186, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI20129347, Length=309, Percent_Identity=31.3915857605178, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24647931, Length=276, Percent_Identity=32.9710144927536, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 32923; Mature: 32923

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNSTLPEHWTAAYIPDQRGRFAVITGTGGLGFEGAVALARSGASVVLAGRNAHKGADAV
CCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCHHHHHHHCCCCEEEEECCCCCCCHHHH
AAIRQAAPASDIRYERLDLACLASVADFAARLRGQMDRLDLLINNAAVMNPPARQETEDG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEECCCCCCCCCCCC
FELQLGTNYLGHFALTGHLLPLLRKGANARVISVSSIAARNGLINLEDLQAEQSYRPGSA
EEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCHHHHHHHHCCCCCHH
YAQSKLACLMFALELHRRSQLGGWGVASIAAHPGVSRTELLHNAPGRGSPASLARSLLWF
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHH
LFQPADQGALPTLFAATWPEAKSGAYYGPHGLSETRGFPAAAQIPPQALDAALSARLWEV
HHCCCCCCCCCCHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SENLTGVTLR
HCCCCCEEEC
>Mature Secondary Structure
MRNSTLPEHWTAAYIPDQRGRFAVITGTGGLGFEGAVALARSGASVVLAGRNAHKGADAV
CCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCHHHHHHHCCCCEEEEECCCCCCCHHHH
AAIRQAAPASDIRYERLDLACLASVADFAARLRGQMDRLDLLINNAAVMNPPARQETEDG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEECCCCCCCCCCCC
FELQLGTNYLGHFALTGHLLPLLRKGANARVISVSSIAARNGLINLEDLQAEQSYRPGSA
EEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCCHHHHHHHHCCCCCHH
YAQSKLACLMFALELHRRSQLGGWGVASIAAHPGVSRTELLHNAPGRGSPASLARSLLWF
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHH
LFQPADQGALPTLFAATWPEAKSGAYYGPHGLSETRGFPAAAQIPPQALDAALSARLWEV
HHCCCCCCCCCCHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SENLTGVTLR
HCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383668 [H]