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The map label for this gene is truB [H]

Identifier: 114799976

GI number: 114799976

Start: 105695

End: 106636

Strand: Direct

Name: truB [H]

Synonym: HNE_0117

Alternate gene names: 114799976

Gene position: 105695-106636 (Clockwise)

Preceding gene: 114800142

Following gene: 114800218

Centisome position: 2.85

GC content: 64.54

Gene sequence:

>942_bases
TTGGCCCGCCAACGTAAGCGCAAAGGCGAGCCCGTTACGGGCTGGATCAACCTTTTCAAGCCGGTGGACATGACCTCCAC
CCAGGCTGTTGCCATCATCAAGCGGCTCTACAATGCCGAGAAGGTGGGCCATGGCGGCACGCTTGATCCGCTGGCAGACG
GTATCCTGCCGATTGCGCTTGGCCAGGCGACCAAGACGGTTCAGTGGGCGATGGACGCGCACAAGGAATATGTCTTCACC
ATCCGCTGGGGTATCTCCACTGCCAGCCAGGACGCCGAAGCCGAGATTACCGCCACCTCGGATGTGCGCCCGGAGCGGGC
TGCCATTGAGGCCGCGTTGACCCGCTATGTCGGCACCATCCAGCAGGTTCCACCCAAATACTCGGCCATCAAGGTGGCCG
GAGAGCGCGCCTATGATCTGGTGCGAGAAGGGGAGGCGTTCGAGCTGGAGGCGCGGGAAGTGGATGTTCACGCGGCGAAT
GTGATCGGGATGCCGGACGCGGATCATACGGTGATCCATGTCGTGTCCGGCAAGGGATTCTATGTGCGCGCCCTGGCGCG
GGATCTGGCCTTTGATCTGGGGGCGGAGGGCCATATCAGCCAGCTGCGCCGCACCCGCGTGGGCGCCTTTGATGCGCCAT
CGGCTGTCTCCATCGAAGCGCTCGAGGCGCTGAAGGAAGACAAGCCCGGCCTGATGGCGATTCTCAAGCCGCTGCACGCG
GTACTGTCAGGCATGCCGCAGGTGCAGGTCAGCCCGCATGACGCGGCCAATATCCGTCAGGGTCGGTCGGTCGTCCTTCT
GCCGCATGTGGTGGAAGGCTGGCGCGCCGGGCGCAGTGAGGAAGACGACCGTGTGGCGCTGGCGCTTGCGGGCGATGAAG
CCATCGCGCTGGGCGAAGTGCGCGCGGGTCAGTTTGAGCCGGTGCGCGTTTTCGCGGGCTGA

Upstream 100 bases:

>100_bases
ATTGACTATGCCGCCGGCTCGCGCATAAGCGCCCCAGCAATCCCTTCCCTCTGCCGCCCATGCGTTCGAACCGCTTGGGC
CGGCCAATCTGGAGATACTT

Downstream 100 bases:

>100_bases
TCAGGTATTGAAGGGCGCGCGCCCGCCCGGCCAGCGGGCAGGATAGAGCAGGGCAGCGATCCTGACAGGCGTGGCCGCCC
GCTCTGCGCGGGCGAGCATC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MARQRKRKGEPVTGWINLFKPVDMTSTQAVAIIKRLYNAEKVGHGGTLDPLADGILPIALGQATKTVQWAMDAHKEYVFT
IRWGISTASQDAEAEITATSDVRPERAAIEAALTRYVGTIQQVPPKYSAIKVAGERAYDLVREGEAFELEAREVDVHAAN
VIGMPDADHTVIHVVSGKGFYVRALARDLAFDLGAEGHISQLRRTRVGAFDAPSAVSIEALEALKEDKPGLMAILKPLHA
VLSGMPQVQVSPHDAANIRQGRSVVLLPHVVEGWRAGRSEEDDRVALALAGDEAIALGEVRAGQFEPVRVFAG

Sequences:

>Translated_313_residues
MARQRKRKGEPVTGWINLFKPVDMTSTQAVAIIKRLYNAEKVGHGGTLDPLADGILPIALGQATKTVQWAMDAHKEYVFT
IRWGISTASQDAEAEITATSDVRPERAAIEAALTRYVGTIQQVPPKYSAIKVAGERAYDLVREGEAFELEAREVDVHAAN
VIGMPDADHTVIHVVSGKGFYVRALARDLAFDLGAEGHISQLRRTRVGAFDAPSAVSIEALEALKEDKPGLMAILKPLHA
VLSGMPQVQVSPHDAANIRQGRSVVLLPHVVEGWRAGRSEEDDRVALALAGDEAIALGEVRAGQFEPVRVFAG
>Mature_312_residues
ARQRKRKGEPVTGWINLFKPVDMTSTQAVAIIKRLYNAEKVGHGGTLDPLADGILPIALGQATKTVQWAMDAHKEYVFTI
RWGISTASQDAEAEITATSDVRPERAAIEAALTRYVGTIQQVPPKYSAIKVAGERAYDLVREGEAFELEAREVDVHAANV
IGMPDADHTVIHVVSGKGFYVRALARDLAFDLGAEGHISQLRRTRVGAFDAPSAVSIEALEALKEDKPGLMAILKPLHAV
LSGMPQVQVSPHDAANIRQGRSVVLLPHVVEGWRAGRSEEDDRVALALAGDEAIALGEVRAGQFEPVRVFAG

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=179, Percent_Identity=33.5195530726257, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI215599015, Length=200, Percent_Identity=30.5, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI4503337, Length=200, Percent_Identity=30.5, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI2367200, Length=272, Percent_Identity=33.8235294117647, Blast_Score=148, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17553978, Length=275, Percent_Identity=25.8181818181818, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323204, Length=255, Percent_Identity=28.6274509803922, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324037, Length=127, Percent_Identity=34.6456692913386, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364189, Length=298, Percent_Identity=26.8456375838926, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281364187, Length=298, Percent_Identity=26.8456375838926, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281364185, Length=298, Percent_Identity=26.8456375838926, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281364183, Length=298, Percent_Identity=26.8456375838926, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI62471759, Length=298, Percent_Identity=26.8456375838926, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17975520, Length=298, Percent_Identity=26.8456375838926, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33818; Mature: 33687

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQRKRKGEPVTGWINLFKPVDMTSTQAVAIIKRLYNAEKVGHGGTLDPLADGILPIAL
CCCCCHHCCCCCEEHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEEE
GQATKTVQWAMDAHKEYVFTIRWGISTASQDAEAEITATSDVRPERAAIEAALTRYVGTI
CCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
QQVPPKYSAIKVAGERAYDLVREGEAFELEAREVDVHAANVIGMPDADHTVIHVVSGKGF
HHCCCCCCEEEECHHHHHHHHHCCCEEEEEEEEEEEEEEEEECCCCCCCEEEEEECCCCE
YVRALARDLAFDLGAEGHISQLRRTRVGAFDAPSAVSIEALEALKEDKPGLMAILKPLHA
EHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
VLSGMPQVQVSPHDAANIRQGRSVVLLPHVVEGWRAGRSEEDDRVALALAGDEAIALGEV
HHCCCCCEEECCCCCHHHHCCCEEEEEHHHHHHHHCCCCCCCCEEEEEEECCCEEEECCC
RAGQFEPVRVFAG
CCCCCCCEEEECC
>Mature Secondary Structure 
ARQRKRKGEPVTGWINLFKPVDMTSTQAVAIIKRLYNAEKVGHGGTLDPLADGILPIAL
CCCCHHCCCCCEEHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEEE
GQATKTVQWAMDAHKEYVFTIRWGISTASQDAEAEITATSDVRPERAAIEAALTRYVGTI
CCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
QQVPPKYSAIKVAGERAYDLVREGEAFELEAREVDVHAANVIGMPDADHTVIHVVSGKGF
HHCCCCCCEEEECHHHHHHHHHCCCEEEEEEEEEEEEEEEEECCCCCCCEEEEEECCCCE
YVRALARDLAFDLGAEGHISQLRRTRVGAFDAPSAVSIEALEALKEDKPGLMAILKPLHA
EHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
VLSGMPQVQVSPHDAANIRQGRSVVLLPHVVEGWRAGRSEEDDRVALALAGDEAIALGEV
HHCCCCCEEECCCCCHHHHCCCEEEEEHHHHHHHHCCCCCCCCEEEEEEECCCEEEECCC
RAGQFEPVRVFAG
CCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA