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The map label for this gene is ptrB [C]

Identifier: 114799975

GI number: 114799975

Start: 24240

End: 26420

Strand: Direct

Name: ptrB [C]

Synonym: HNE_0034

Alternate gene names: 114799975

Gene position: 24240-26420 (Clockwise)

Preceding gene: 114797256

Following gene: 114799210

Centisome position: 0.65

GC content: 62.82

Gene sequence:

>2181_bases
ATGCGCACACTCATTGTGTCGCTGAGCGCCTTGCTTCTGGCGGCTTGTGCGTCAGACCCCCAACCGGAGACAAACTTGAC
CGCTGACAATCCGACATCCGCCGAGACCGCGTCCGTACCCTTCGACGCGGCTTCCCATCTGTGGCTTGAAGAAGTGGAAG
GGGAGGCGGCCCTCGCCTGGGTCACCGCGCAGAATGAGCGCACCCTCGCTGAGCTTGAAGCTGACCCGCGCTACGCCCCT
TTCGAAGCCGCGGCCCTTGAGGTCTTGAATGCCTCTGAACGCATTCCCTACGGCACCGTGCGCGATGGATACGTCTATAA
CTTCTGGCAGGACGAGACGAATGTCCGCGGCCTGTGGCGCCGCACGCCGCTGGGCAGCTATGGAAGCGATACGCCTGCCT
GGGAAACCATCGTCGATTTCGACAAGCTCGCCGCCGAAGAAGGCGCCAATTGGGTCTATAAAGGCGCTGACTGCCTGCGG
CCGGCGCCGGGCGGGGAATGGCGCTGCCTCGTCTCCCTGTCAGACGGCGGCAAGGACGCCGTCGTCACCCGTGAGTTCAA
CCTGTCCACCAAAACCTTCGTTGAGGACGGCTTCATCACGCCCGAAGCCAAACAGGGCCTCGCCTGGGCCGGTCCCGACA
CGCTGCTGATCGCCACCGACTGGGGCGCAGGCTCGCTCACCGAGTCCGGCTATCCCTTCATCGTCAAGCGCTGGCAGCGC
GGCACCCCGCTGGAAAGCGCGCAGGAGCTGATCCGCGGCAAGGAAACCGATGTTGGCGTCTGGCCGATGACGATGAAACT
GGAAGACGGCCGCATCCTGCAAGGCGCCGTACAGGCCGAAACCTTCTTCACCTCGAAATACTTCTGGTTCCCGGAGGGCG
AAAACGCCGCCGTGCAATGGCCGCTGCCGCCCAAGGCGAGCCCCAATGGCATCTACAAAGGCCAGTTCCTGTTCTCCATC
GAACAGGACTGGGCGCCTGAAGGTCAGGGCCCGTTCCAGGCCGGTGACCTTCTCGCCTTTGATCTTGAGACCTTCCTCGA
AACCCGCGCGCTGCCCCCCGTTACGCTCGCCTTCCGCCCGTCTGAAACTCAGGCGGTCAATGGCGTCGCTGTCGCCAAGG
GCGCCGCGCTTCTCTCGATCAATGACAATGTCGTCGGAAAGCTTCTGCGCATTGAACCCTCCGCCAGCGGATGGACGACA
ACGCCTATAGAGCTGCCCGGCACAGGCCAGGTCGGCATCACCTTTGCCGATGAAAACGAAACCACCGTCTTCCTCAATTA
CGAAGACTTCCTGACCCCAAGCTCGCTCCTCAACTATGAGGTCGCCACCGGCGCCGTCACGCCGCTCAAATCCCTGCCCG
CCAAATTCGACACCGCCGGCCTCACAGTCGAGCAGTTCTTCGCCACCTCGAAAGATGGCACGCGCGTGCCCTATTTCATC
GTCCACCGGGAAGACATTCCGCTGGATGGGACAACGCCTGCGCTGCTCTATGGTTATGGCGGCTTCCAGGTCTCGATGAA
CCCCGGCTATAGCCCCGTTTCCGGGCGCCTGTGGCTGGAGCAGGGCGGGGCCTATATCCTGGCCAACATCCGCGGCGGCG
GCGAATTCGGGCCAAACTGGCACCAGGCCGGCCTGAAACTCAACCGCCAGCGCATCTATGACGACTTCATTGCTGTCGGC
GAAGACGTGATCGCTCGCAACATCACCAGCCCGGAGCATCTGGGCATCATGGGCGGCTCCAATGGCGGCCTTCTGATGGG
CGTGATGCTCAATCAGCGCCCGGATCTCTGGAACGCCGTCGTCGTGCAGGTCCCCCTGCTCGACATGCTGCGCTACCATC
TGCTTCTGGCGGGCGCGTCCTGGGTCGATGAATATGGCTCCCCCGATGTGCCCGAGGAACGCGCCTTCCTCGAAACCATC
TCGCCCTACCAGAACTTTGACGCGTCGAAGGATTATCCCGTCCCCTTCTTCGTCACCTCGACGAAGGATGACCGCGTCCA
TCCCGGCCATGCCCGCAAGATGGCAAAGCGCTTCGAGGAGGCAGGTCTGCCCTTCTACTATTTCGAAAACATGGATGGGG
GCCACGCCGCCGCCGCTGACCAGACGGCCCGCGCCAAACGCTCCGCCCTCGAATTCACCTATCTTGCCCGGCAACTGTTC
CCGGCGCCGCCTGAAGAGTAA

Upstream 100 bases:

>100_bases
AGGATCACGGGCCTGCGGATAACAAACGCCCGACCGTCCACAGACCCAGGGTGTTCTCACCACTGCGTTGCAGACCCATA
TGATCAGAGAGGAGACACCC

Downstream 100 bases:

>100_bases
GGGCGGACAAACCCGCTCACGCAAAGATGAGGACCGGCCAGCACCCCTGGCCGGTCCTTTCTTTTGCGGCCGCCTTCAAC
CTGTGGCATATTCAGTCCAT

Product: prolyl oligopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 726; Mature: 726

Protein sequence:

>726_residues
MRTLIVSLSALLLAACASDPQPETNLTADNPTSAETASVPFDAASHLWLEEVEGEAALAWVTAQNERTLAELEADPRYAP
FEAAALEVLNASERIPYGTVRDGYVYNFWQDETNVRGLWRRTPLGSYGSDTPAWETIVDFDKLAAEEGANWVYKGADCLR
PAPGGEWRCLVSLSDGGKDAVVTREFNLSTKTFVEDGFITPEAKQGLAWAGPDTLLIATDWGAGSLTESGYPFIVKRWQR
GTPLESAQELIRGKETDVGVWPMTMKLEDGRILQGAVQAETFFTSKYFWFPEGENAAVQWPLPPKASPNGIYKGQFLFSI
EQDWAPEGQGPFQAGDLLAFDLETFLETRALPPVTLAFRPSETQAVNGVAVAKGAALLSINDNVVGKLLRIEPSASGWTT
TPIELPGTGQVGITFADENETTVFLNYEDFLTPSSLLNYEVATGAVTPLKSLPAKFDTAGLTVEQFFATSKDGTRVPYFI
VHREDIPLDGTTPALLYGYGGFQVSMNPGYSPVSGRLWLEQGGAYILANIRGGGEFGPNWHQAGLKLNRQRIYDDFIAVG
EDVIARNITSPEHLGIMGGSNGGLLMGVMLNQRPDLWNAVVVQVPLLDMLRYHLLLAGASWVDEYGSPDVPEERAFLETI
SPYQNFDASKDYPVPFFVTSTKDDRVHPGHARKMAKRFEEAGLPFYYFENMDGGHAAAADQTARAKRSALEFTYLARQLF
PAPPEE

Sequences:

>Translated_726_residues
MRTLIVSLSALLLAACASDPQPETNLTADNPTSAETASVPFDAASHLWLEEVEGEAALAWVTAQNERTLAELEADPRYAP
FEAAALEVLNASERIPYGTVRDGYVYNFWQDETNVRGLWRRTPLGSYGSDTPAWETIVDFDKLAAEEGANWVYKGADCLR
PAPGGEWRCLVSLSDGGKDAVVTREFNLSTKTFVEDGFITPEAKQGLAWAGPDTLLIATDWGAGSLTESGYPFIVKRWQR
GTPLESAQELIRGKETDVGVWPMTMKLEDGRILQGAVQAETFFTSKYFWFPEGENAAVQWPLPPKASPNGIYKGQFLFSI
EQDWAPEGQGPFQAGDLLAFDLETFLETRALPPVTLAFRPSETQAVNGVAVAKGAALLSINDNVVGKLLRIEPSASGWTT
TPIELPGTGQVGITFADENETTVFLNYEDFLTPSSLLNYEVATGAVTPLKSLPAKFDTAGLTVEQFFATSKDGTRVPYFI
VHREDIPLDGTTPALLYGYGGFQVSMNPGYSPVSGRLWLEQGGAYILANIRGGGEFGPNWHQAGLKLNRQRIYDDFIAVG
EDVIARNITSPEHLGIMGGSNGGLLMGVMLNQRPDLWNAVVVQVPLLDMLRYHLLLAGASWVDEYGSPDVPEERAFLETI
SPYQNFDASKDYPVPFFVTSTKDDRVHPGHARKMAKRFEEAGLPFYYFENMDGGHAAAADQTARAKRSALEFTYLARQLF
PAPPEE
>Mature_726_residues
MRTLIVSLSALLLAACASDPQPETNLTADNPTSAETASVPFDAASHLWLEEVEGEAALAWVTAQNERTLAELEADPRYAP
FEAAALEVLNASERIPYGTVRDGYVYNFWQDETNVRGLWRRTPLGSYGSDTPAWETIVDFDKLAAEEGANWVYKGADCLR
PAPGGEWRCLVSLSDGGKDAVVTREFNLSTKTFVEDGFITPEAKQGLAWAGPDTLLIATDWGAGSLTESGYPFIVKRWQR
GTPLESAQELIRGKETDVGVWPMTMKLEDGRILQGAVQAETFFTSKYFWFPEGENAAVQWPLPPKASPNGIYKGQFLFSI
EQDWAPEGQGPFQAGDLLAFDLETFLETRALPPVTLAFRPSETQAVNGVAVAKGAALLSINDNVVGKLLRIEPSASGWTT
TPIELPGTGQVGITFADENETTVFLNYEDFLTPSSLLNYEVATGAVTPLKSLPAKFDTAGLTVEQFFATSKDGTRVPYFI
VHREDIPLDGTTPALLYGYGGFQVSMNPGYSPVSGRLWLEQGGAYILANIRGGGEFGPNWHQAGLKLNRQRIYDDFIAVG
EDVIARNITSPEHLGIMGGSNGGLLMGVMLNQRPDLWNAVVVQVPLLDMLRYHLLLAGASWVDEYGSPDVPEERAFLETI
SPYQNFDASKDYPVPFFVTSTKDDRVHPGHARKMAKRFEEAGLPFYYFENMDGGHAAAADQTARAKRSALEFTYLARQLF
PAPPEE

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=372, Percent_Identity=32.5268817204301, Blast_Score=211, Evalue=3e-54,
Organism=Homo sapiens, GI108860686, Length=203, Percent_Identity=27.5862068965517, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI284172438, Length=203, Percent_Identity=27.5862068965517, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI284172431, Length=203, Percent_Identity=27.5862068965517, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI284172420, Length=203, Percent_Identity=27.5862068965517, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI284172413, Length=203, Percent_Identity=27.5862068965517, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI70778815, Length=203, Percent_Identity=27.5862068965517, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI108860692, Length=200, Percent_Identity=27, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI1788150, Length=310, Percent_Identity=29.3548387096774, Blast_Score=137, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24583414, Length=375, Percent_Identity=33.3333333333333, Blast_Score=208, Evalue=8e-54,
Organism=Drosophila melanogaster, GI221510989, Length=283, Percent_Identity=36.3957597173145, Blast_Score=186, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 79548; Mature: 79548

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLIVSLSALLLAACASDPQPETNLTADNPTSAETASVPFDAASHLWLEEVEGEAALAW
CCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEE
VTAQNERTLAELEADPRYAPFEAAALEVLNASERIPYGTVRDGYVYNFWQDETNVRGLWR
EEECCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCEEEE
RTPLGSYGSDTPAWETIVDFDKLAAEEGANWVYKGADCLRPAPGGEWRCLVSLSDGGKDA
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCCEEEEEEECCCCCCE
VVTREFNLSTKTFVEDGFITPEAKQGLAWAGPDTLLIATDWGAGSLTESGYPFIVKRWQR
EEEEEECCCHHHEECCCCCCCHHHCCCEECCCCEEEEEECCCCCCCCCCCCCCEEEECCC
GTPLESAQELIRGKETDVGVWPMTMKLEDGRILQGAVQAETFFTSKYFWFPEGENAAVQW
CCCHHHHHHHHCCCCCCCEEEEEEEEECCCCEEEHHHHHEEEEEEEEEEEECCCCCEEEC
PLPPKASPNGIYKGQFLFSIEQDWAPEGQGPFQAGDLLAFDLETFLETRALPPVTLAFRP
CCCCCCCCCCEEECEEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHCCCCCEEEEECC
SETQAVNGVAVAKGAALLSINDNVVGKLLRIEPSASGWTTTPIELPGTGQVGITFADENE
CCCCCCCCEEEECCEEEEEECCHHEEEEEEECCCCCCCEECCEECCCCCEEEEEEECCCC
TTVFLNYEDFLTPSSLLNYEVATGAVTPLKSLPAKFDTAGLTVEQFFATSKDGTRVPYFI
EEEEEEHHHHCCHHHHHCEEECCCCCCCHHHCCCCCCCCCCCHHHHHHCCCCCCCCCEEE
VHREDIPLDGTTPALLYGYGGFQVSMNPGYSPVSGRLWLEQGGAYILANIRGGGEFGPNW
EECCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCH
HQAGLKLNRQRIYDDFIAVGEDVIARNITSPEHLGIMGGSNGGLLMGVMLNQRPDLWNAV
HHHCCEECHHHHHHHHHHHCCHHHHHCCCCCHHEEEEECCCCCEEEEEEECCCCCHHCEE
VVQVPLLDMLRYHLLLAGASWVDEYGSPDVPEERAFLETISPYQNFDASKDYPVPFFVTS
EEEHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHCCHHHCCCCCCCCCCEEEEEC
TKDDRVHPGHARKMAKRFEEAGLPFYYFENMDGGHAAAADQTARAKRSALEFTYLARQLF
CCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
PAPPEE
CCCCCC
>Mature Secondary Structure
MRTLIVSLSALLLAACASDPQPETNLTADNPTSAETASVPFDAASHLWLEEVEGEAALAW
CCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEE
VTAQNERTLAELEADPRYAPFEAAALEVLNASERIPYGTVRDGYVYNFWQDETNVRGLWR
EEECCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCEEEE
RTPLGSYGSDTPAWETIVDFDKLAAEEGANWVYKGADCLRPAPGGEWRCLVSLSDGGKDA
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCCEEEEEEECCCCCCE
VVTREFNLSTKTFVEDGFITPEAKQGLAWAGPDTLLIATDWGAGSLTESGYPFIVKRWQR
EEEEEECCCHHHEECCCCCCCHHHCCCEECCCCEEEEEECCCCCCCCCCCCCCEEEECCC
GTPLESAQELIRGKETDVGVWPMTMKLEDGRILQGAVQAETFFTSKYFWFPEGENAAVQW
CCCHHHHHHHHCCCCCCCEEEEEEEEECCCCEEEHHHHHEEEEEEEEEEEECCCCCEEEC
PLPPKASPNGIYKGQFLFSIEQDWAPEGQGPFQAGDLLAFDLETFLETRALPPVTLAFRP
CCCCCCCCCCEEECEEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHCCCCCEEEEECC
SETQAVNGVAVAKGAALLSINDNVVGKLLRIEPSASGWTTTPIELPGTGQVGITFADENE
CCCCCCCCEEEECCEEEEEECCHHEEEEEEECCCCCCCEECCEECCCCCEEEEEEECCCC
TTVFLNYEDFLTPSSLLNYEVATGAVTPLKSLPAKFDTAGLTVEQFFATSKDGTRVPYFI
EEEEEEHHHHCCHHHHHCEEECCCCCCCHHHCCCCCCCCCCCHHHHHHCCCCCCCCCEEE
VHREDIPLDGTTPALLYGYGGFQVSMNPGYSPVSGRLWLEQGGAYILANIRGGGEFGPNW
EECCCCCCCCCCCEEEEECCCEEEEECCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCH
HQAGLKLNRQRIYDDFIAVGEDVIARNITSPEHLGIMGGSNGGLLMGVMLNQRPDLWNAV
HHHCCEECHHHHHHHHHHHCCHHHHHCCCCCHHEEEEECCCCCEEEEEEECCCCCHHCEE
VVQVPLLDMLRYHLLLAGASWVDEYGSPDVPEERAFLETISPYQNFDASKDYPVPFFVTS
EEEHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHCCHHHCCCCCCCCCCEEEEEC
TKDDRVHPGHARKMAKRFEEAGLPFYYFENMDGGHAAAADQTARAKRSALEFTYLARQLF
CCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
PAPPEE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.23 {tosyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 80 {acetyl-tyrosine} 0.47 {tosyl-Lys-methyl} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]