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The map label for this gene is cobA [H]

Identifier: 114799955

GI number: 114799955

Start: 2331874

End: 2332653

Strand: Reverse

Name: cobA [H]

Synonym: HNE_2230

Alternate gene names: 114799955

Gene position: 2332653-2331874 (Counterclockwise)

Preceding gene: 114800210

Following gene: 114798645

Centisome position: 62.96

GC content: 66.67

Gene sequence:

>780_bases
ATGACCGTCCGTATCACCCTGGTTGGGGCTGGCCCAGGCGCGGCCGATCTCCTGACGCTTCGCGCCGTGCGGGCGATCGA
GGCGGCCGAGGTCGTCCTGATCGATGCGCTGGTGAGCGATGAGGTCAGATCGCTCATTCCTCCCGGCGCCCGGATCATTG
AGGTTGGCAAGCGCGGGCACAAGCCTTCGGCCGGACAGGATTTCATCAACCGGACTCTGGTTCGATATGCGCAGCAGGGG
TTCAATGTGGTCCGGCTGAAGGGCGGCGATCCTTCCATCTTTGGACGGGCGGCCGAGGAGCGGGCCGCGCTTGAGGCTAT
GGGAATCGAGGTTGCCGTCGTTCCCGGAATAACCAGCGCATCGGCGGCCGCTGCGCAGTTTCGAATCTCTCTTACCGAAC
GGGGCACGGCCCGGCGGGTCCTGTTCGCAACGGGGCACCGGGCAGAGGGACCGGAAACCGACTGGGAGAGCGCCGCGGAT
CCTGCCACGACGGTATGCCTCTATATGGCCGCACATGATTTTGCGGCCATCGCGGCCGCGTTGATTGCGGCGGGCCGTCG
GGCGGACACACCGGCCATCGCTGTCTGCGATGTGGAGCGACCGGCCGCAAGGATGCTGGCGGCGACCCTTGCAACGCTGG
CTGCCCGGCTCGCGGCCGACGCGTTTGAAGGGCCCGTCGTCATCCTGGTCGGCGACGTGTGCCGCAGGGCCGTTAGCTCA
CCCTGCGCACCGCATTTCCGAGAGGGTGAGCGACGGGTCAGCCTGCCTGGTTTGCTCTAA

Upstream 100 bases:

>100_bases
GGGATTTATCCGGTGTGGTAAAACTCGCCCGCTATAACGGCGCAGGGGGGCTGTCAGACAGGACGCGTTTCTGGCTGGGG
TTTACCTGGAAGGCCTGGCA

Downstream 100 bases:

>100_bases
GCTAATTGATCGCGTCCTGCCCGGATGAGGCCCCGATCAGACAGACAGGGGGCCGGTCACAGACCGTTGGCCCGGTCCTG
GACCGCGGAGTTGCCAAGCC

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTVRITLVGAGPGAADLLTLRAVRAIEAAEVVLIDALVSDEVRSLIPPGARIIEVGKRGHKPSAGQDFINRTLVRYAQQG
FNVVRLKGGDPSIFGRAAEERAALEAMGIEVAVVPGITSASAAAAQFRISLTERGTARRVLFATGHRAEGPETDWESAAD
PATTVCLYMAAHDFAAIAAALIAAGRRADTPAIAVCDVERPAARMLAATLATLAARLAADAFEGPVVILVGDVCRRAVSS
PCAPHFREGERRVSLPGLL

Sequences:

>Translated_259_residues
MTVRITLVGAGPGAADLLTLRAVRAIEAAEVVLIDALVSDEVRSLIPPGARIIEVGKRGHKPSAGQDFINRTLVRYAQQG
FNVVRLKGGDPSIFGRAAEERAALEAMGIEVAVVPGITSASAAAAQFRISLTERGTARRVLFATGHRAEGPETDWESAAD
PATTVCLYMAAHDFAAIAAALIAAGRRADTPAIAVCDVERPAARMLAATLATLAARLAADAFEGPVVILVGDVCRRAVSS
PCAPHFREGERRVSLPGLL
>Mature_258_residues
TVRITLVGAGPGAADLLTLRAVRAIEAAEVVLIDALVSDEVRSLIPPGARIIEVGKRGHKPSAGQDFINRTLVRYAQQGF
NVVRLKGGDPSIFGRAAEERAALEAMGIEVAVVPGITSASAAAAQFRISLTERGTARRVLFATGHRAEGPETDWESAADP
ATTVCLYMAAHDFAAIAAALIAAGRRADTPAIAVCDVERPAARMLAATLATLAARLAADAFEGPVVILVGDVCRRAVSSP
CAPHFREGERRVSLPGLL

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=229, Percent_Identity=41.9213973799127, Blast_Score=157, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6322922, Length=230, Percent_Identity=33.0434782608696, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 27020; Mature: 26889

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRITLVGAGPGAADLLTLRAVRAIEAAEVVLIDALVSDEVRSLIPPGARIIEVGKRGH
CEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHCCCCC
KPSAGQDFINRTLVRYAQQGFNVVRLKGGDPSIFGRAAEERAALEAMGIEVAVVPGITSA
CCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCHHHHHHHHHHHCEEEEEECCCCCC
SAAAAQFRISLTERGTARRVLFATGHRAEGPETDWESAADPATTVCLYMAAHDFAAIAAA
HHHHHEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LIAAGRRADTPAIAVCDVERPAARMLAATLATLAARLAADAFEGPVVILVGDVCRRAVSS
HHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCC
PCAPHFREGERRVSLPGLL
CCCCCHHCCCCEECCCCCC
>Mature Secondary Structure 
TVRITLVGAGPGAADLLTLRAVRAIEAAEVVLIDALVSDEVRSLIPPGARIIEVGKRGH
EEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHCCCCC
KPSAGQDFINRTLVRYAQQGFNVVRLKGGDPSIFGRAAEERAALEAMGIEVAVVPGITSA
CCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHCCHHHHHHHHHHHCEEEEEECCCCCC
SAAAAQFRISLTERGTARRVLFATGHRAEGPETDWESAADPATTVCLYMAAHDFAAIAAA
HHHHHEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LIAAGRRADTPAIAVCDVERPAARMLAATLATLAARLAADAFEGPVVILVGDVCRRAVSS
HHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCC
PCAPHFREGERRVSLPGLL
CCCCCHHCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]