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The map label for this gene is thiC [H]

Identifier: 114799796

GI number: 114799796

Start: 3488534

End: 3490426

Strand: Direct

Name: thiC [H]

Synonym: HNE_3338

Alternate gene names: 114799796

Gene position: 3488534-3490426 (Clockwise)

Preceding gene: 114797390

Following gene: 114797060

Centisome position: 94.16

GC content: 62.28

Gene sequence:

>1893_bases
ATGAACAAGCCCACCAAGCCCGGCGATTTCGACGCCCCCAAAGTCACCACCGGCGCCCTGCCCGCCAGCCGCAAGGTCTA
TTCGCGCCCCGCGCGCGACCCGGACCTCGCCGTGCCCCACCGCGAGATCGATCTTCATCCGTCAGCGAATGAACCCGCCG
TCCGCGTCTACGACACCTCCGGCCCCTATTCCGACCCCACCGCAGAGATCGACGTGGAGAAGGGCCTCCCCCGCCATCGG
CGCGACTGGCTGCTGGCGCGCGGCGGCCTGGAGGAATATGACGGCCGGGACGTGAAGCCCGAGGACAATGGCGGCGCCAC
CGGCAAATACCTCGCCCGAGAGTTCCCGGTGAAACACATGCCCCTGCGCGGCCTGCCTGGCCAAATGGTGACCCAGTACG
AGTTTGCCAAAGCCGGCATCATCACAAAGGAGATGGTATACGTCGCCGAGCGCGAAAACCTTGGCCGCAGCCAGGCGCTG
GAAAATGCCCGCGCGCAGATCGACCAGGGCAATTCCTTCGGTGCCGACATCCCCGAATTCATCACGCCCGAATTCGTCCG
TGACGAGATTGCCCGTGGCCGCGCCATCATTCCGGCCAATATCAACCATGCCGAGCTGGAGCCTCAGATCATCGGGCGCA
ACTTCCTTGTGAAGATCAATGCCAATATCGGCAATTCGGCGGTCGCTTCCTCCATCGAGGAAGAAGTGGAGAAAATGGTC
TGGGCCACCCGCTGGGGCGCCGACAATGTGATGGACCTCTCCACGGGACGCAACATCCACAACACCCGCGAATGGATCAT
CCGCAACAGCCCCGTTCCGATCGGCACCGTGCCGATCTATCAGGCGCTGGAGAAGGTCAACGGCGTCGCCGAAGACCTCA
CCTGGGAAGTCTACCGCGACACGCTGATCGAGCAGTGCGAACAGGGCGTCGACTATTTCACCATCCATGCCGGTGTGCGC
CTCGCCTATATCCACCTCACGGCCAAGCGCGTCTGCGGCATCGTCTCGCGCGGCGGCTCGATCATGGCGAAATGGATGCT
CGCCCATCATAAGGAGAGCTTCCTCTACGATCATTTCGAGGACATTTGCGACATCATGCGCGCCTATGACGTCTCATTCT
CATTGGGGGACGGCCTGCGCCCCGGCGCCATCGCGGACGCGAACGACGCGGCGCAGTTTGCAGAGCTGGAAACCCTCGGT
GAGCTGACGAAGATCGCCTGGGCCAAGGGCTGTCAGGTGATGATCGAAGGGCCCGGCCATGTGCCGATGCACAAGATCAA
GATCAATATGGACAAGCAGCTCAAAGCTTGCGGCGAGGCCCCATTCTACACGCTCGGGCCGCTCGTCACCGATATTGCGC
CGGGTTATGATCATATCACGTCGGGCATTGGCGCGGCGATGATCGGCTGGTTTGGCACGTCCATGCTCTGCTATGTGACG
CCCAAGGAGCATTTGGGGCTGCCCGACCGGAATGATGTGAAGGTTGGCGTGATCACCTACCGTATCGCGGCCCATGCCGC
CGACCTTGCCAAGGGCCACCCCGCCGCGCAGCTGCGCGACGATGCCCTCTCCCGCGCGCGGTTCGAGTTCCGCTGGGAAG
ACCAGTTCAATCTGGCGCTCGACCCCGAAACCGCGCGGGACTTCCACGACCAGACCCTGCCGAAAGACGCACATAAAGTG
GCCCATTTCTGCTCCATGTGCGGCCCGAAATTCTGCTCGATGAAAATCACCGCCGAAGTCCGCGACTACGCCGCCGGAAT
GTCCGAGAACGAACGTCTGGACCTGGAGAAACAGGCCGCCGAAGCCCGCAAGGGCATGGAGGAAAAGAGCCGCGAGTTCC
TGAACAAGGGAGGGGAGATTTACATACACGCTCCGAAGGACCGCCAAACGTGA

Upstream 100 bases:

>100_bases
ACCGCGAGATCCGCCTTCCCCTCGGCGCGCCCCTGCCCGCCGCCCCGCTCCGCCACTGCATCGCCATGGCCCTCACCTAT
CACAAGAGAAAGTCCGTCTC

Downstream 100 bases:

>100_bases
CTTTACGGCTTCGTTTGAACCATACCCTACTCCAAGACGAATGCGCATTGAAGAGGCAGTAATTTGAAACCCATCGAGAA
CGTCTCGCAGTTCCTAAGCG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MNKPTKPGDFDAPKVTTGALPASRKVYSRPARDPDLAVPHREIDLHPSANEPAVRVYDTSGPYSDPTAEIDVEKGLPRHR
RDWLLARGGLEEYDGRDVKPEDNGGATGKYLAREFPVKHMPLRGLPGQMVTQYEFAKAGIITKEMVYVAERENLGRSQAL
ENARAQIDQGNSFGADIPEFITPEFVRDEIARGRAIIPANINHAELEPQIIGRNFLVKINANIGNSAVASSIEEEVEKMV
WATRWGADNVMDLSTGRNIHNTREWIIRNSPVPIGTVPIYQALEKVNGVAEDLTWEVYRDTLIEQCEQGVDYFTIHAGVR
LAYIHLTAKRVCGIVSRGGSIMAKWMLAHHKESFLYDHFEDICDIMRAYDVSFSLGDGLRPGAIADANDAAQFAELETLG
ELTKIAWAKGCQVMIEGPGHVPMHKIKINMDKQLKACGEAPFYTLGPLVTDIAPGYDHITSGIGAAMIGWFGTSMLCYVT
PKEHLGLPDRNDVKVGVITYRIAAHAADLAKGHPAAQLRDDALSRARFEFRWEDQFNLALDPETARDFHDQTLPKDAHKV
AHFCSMCGPKFCSMKITAEVRDYAAGMSENERLDLEKQAAEARKGMEEKSREFLNKGGEIYIHAPKDRQT

Sequences:

>Translated_630_residues
MNKPTKPGDFDAPKVTTGALPASRKVYSRPARDPDLAVPHREIDLHPSANEPAVRVYDTSGPYSDPTAEIDVEKGLPRHR
RDWLLARGGLEEYDGRDVKPEDNGGATGKYLAREFPVKHMPLRGLPGQMVTQYEFAKAGIITKEMVYVAERENLGRSQAL
ENARAQIDQGNSFGADIPEFITPEFVRDEIARGRAIIPANINHAELEPQIIGRNFLVKINANIGNSAVASSIEEEVEKMV
WATRWGADNVMDLSTGRNIHNTREWIIRNSPVPIGTVPIYQALEKVNGVAEDLTWEVYRDTLIEQCEQGVDYFTIHAGVR
LAYIHLTAKRVCGIVSRGGSIMAKWMLAHHKESFLYDHFEDICDIMRAYDVSFSLGDGLRPGAIADANDAAQFAELETLG
ELTKIAWAKGCQVMIEGPGHVPMHKIKINMDKQLKACGEAPFYTLGPLVTDIAPGYDHITSGIGAAMIGWFGTSMLCYVT
PKEHLGLPDRNDVKVGVITYRIAAHAADLAKGHPAAQLRDDALSRARFEFRWEDQFNLALDPETARDFHDQTLPKDAHKV
AHFCSMCGPKFCSMKITAEVRDYAAGMSENERLDLEKQAAEARKGMEEKSREFLNKGGEIYIHAPKDRQT
>Mature_630_residues
MNKPTKPGDFDAPKVTTGALPASRKVYSRPARDPDLAVPHREIDLHPSANEPAVRVYDTSGPYSDPTAEIDVEKGLPRHR
RDWLLARGGLEEYDGRDVKPEDNGGATGKYLAREFPVKHMPLRGLPGQMVTQYEFAKAGIITKEMVYVAERENLGRSQAL
ENARAQIDQGNSFGADIPEFITPEFVRDEIARGRAIIPANINHAELEPQIIGRNFLVKINANIGNSAVASSIEEEVEKMV
WATRWGADNVMDLSTGRNIHNTREWIIRNSPVPIGTVPIYQALEKVNGVAEDLTWEVYRDTLIEQCEQGVDYFTIHAGVR
LAYIHLTAKRVCGIVSRGGSIMAKWMLAHHKESFLYDHFEDICDIMRAYDVSFSLGDGLRPGAIADANDAAQFAELETLG
ELTKIAWAKGCQVMIEGPGHVPMHKIKINMDKQLKACGEAPFYTLGPLVTDIAPGYDHITSGIGAAMIGWFGTSMLCYVT
PKEHLGLPDRNDVKVGVITYRIAAHAADLAKGHPAAQLRDDALSRARFEFRWEDQFNLALDPETARDFHDQTLPKDAHKV
AHFCSMCGPKFCSMKITAEVRDYAAGMSENERLDLEKQAAEARKGMEEKSREFLNKGGEIYIHAPKDRQT

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=615, Percent_Identity=64.8780487804878, Blast_Score=802, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 70044; Mature: 70044

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPTKPGDFDAPKVTTGALPASRKVYSRPARDPDLAVPHREIDLHPSANEPAVRVYDTS
CCCCCCCCCCCCCCEECCCCCCCHHHHCCCCCCCCCCCCCCEEECCCCCCCCEEEEEECC
GPYSDPTAEIDVEKGLPRHRRDWLLARGGLEEYDGRDVKPEDNGGATGKYLAREFPVKHM
CCCCCCCCEEEHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCC
PLRGLPGQMVTQYEFAKAGIITKEMVYVAERENLGRSQALENARAQIDQGNSFGADIPEF
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHH
ITPEFVRDEIARGRAIIPANINHAELEPQIIGRNFLVKINANIGNSAVASSIEEEVEKMV
CCHHHHHHHHHCCCEEEECCCCHHHCCCEEECCEEEEEEECCCCCHHHHHHHHHHHHHHH
WATRWGADNVMDLSTGRNIHNTREWIIRNSPVPIGTVPIYQALEKVNGVAEDLTWEVYRD
HHHCCCCCCEEECCCCCCCCCCHHHEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHH
TLIEQCEQGVDYFTIHAGVRLAYIHLTAKRVCGIVSRGGSIMAKWMLAHHKESFLYDHFE
HHHHHHHCCCCEEEEECCCEEEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
DICDIMRAYDVSFSLGDGLRPGAIADANDAAQFAELETLGELTKIAWAKGCQVMIEGPGH
HHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
VPMHKIKINMDKQLKACGEAPFYTLGPLVTDIAPGYDHITSGIGAAMIGWFGTSMLCYVT
CCEEEEEECCCHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEC
PKEHLGLPDRNDVKVGVITYRIAAHAADLAKGHPAAQLRDDALSRARFEFRWEDQFNLAL
CHHHCCCCCCCCEEEEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHCEEEEEECCCCCEEE
DPETARDFHDQTLPKDAHKVAHFCSMCGPKFCSMKITAEVRDYAAGMSENERLDLEKQAA
CCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHCCCCCCCCCCHHHHHH
EARKGMEEKSREFLNKGGEIYIHAPKDRQT
HHHHCHHHHHHHHHHCCCEEEEECCCCCCC
>Mature Secondary Structure
MNKPTKPGDFDAPKVTTGALPASRKVYSRPARDPDLAVPHREIDLHPSANEPAVRVYDTS
CCCCCCCCCCCCCCEECCCCCCCHHHHCCCCCCCCCCCCCCEEECCCCCCCCEEEEEECC
GPYSDPTAEIDVEKGLPRHRRDWLLARGGLEEYDGRDVKPEDNGGATGKYLAREFPVKHM
CCCCCCCCEEEHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCC
PLRGLPGQMVTQYEFAKAGIITKEMVYVAERENLGRSQALENARAQIDQGNSFGADIPEF
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHH
ITPEFVRDEIARGRAIIPANINHAELEPQIIGRNFLVKINANIGNSAVASSIEEEVEKMV
CCHHHHHHHHHCCCEEEECCCCHHHCCCEEECCEEEEEEECCCCCHHHHHHHHHHHHHHH
WATRWGADNVMDLSTGRNIHNTREWIIRNSPVPIGTVPIYQALEKVNGVAEDLTWEVYRD
HHHCCCCCCEEECCCCCCCCCCHHHEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHH
TLIEQCEQGVDYFTIHAGVRLAYIHLTAKRVCGIVSRGGSIMAKWMLAHHKESFLYDHFE
HHHHHHHCCCCEEEEECCCEEEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
DICDIMRAYDVSFSLGDGLRPGAIADANDAAQFAELETLGELTKIAWAKGCQVMIEGPGH
HHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
VPMHKIKINMDKQLKACGEAPFYTLGPLVTDIAPGYDHITSGIGAAMIGWFGTSMLCYVT
CCEEEEEECCCHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEC
PKEHLGLPDRNDVKVGVITYRIAAHAADLAKGHPAAQLRDDALSRARFEFRWEDQFNLAL
CHHHCCCCCCCCEEEEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHCEEEEEECCCCCEEE
DPETARDFHDQTLPKDAHKVAHFCSMCGPKFCSMKITAEVRDYAAGMSENERLDLEKQAA
CCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHCCCCCCCCCCHHHHHH
EARKGMEEKSREFLNKGGEIYIHAPKDRQT
HHHHCHHHHHHHHHHCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA