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The map label for this gene is menB [C]

Identifier: 114799733

GI number: 114799733

Start: 2251987

End: 2252895

Strand: Reverse

Name: menB [C]

Synonym: HNE_2166

Alternate gene names: 114799733

Gene position: 2252895-2251987 (Counterclockwise)

Preceding gene: 114799121

Following gene: 114798747

Centisome position: 60.81

GC content: 60.73

Gene sequence:

>909_bases
ATGGCACGGCCCAAGGAATTCAAGGAAATCATTCTCGACATCGAGGACGGCGTCGCCACCCTCACGCTGCACCGTCCCGA
CAACATGAACGCTTTCACCGGCACCATGATGTACGAAATGATCGAAGCCTTCGACATCACCGACGCCAATGACGATGTGA
AGGTCGTCATTGTGACCGGCCATGGCGACCGCGCTTTCTGCGCCGGCGCAGACCTCTCTGCAGGTGCCAAGACGTTCGAC
TATGACGCCCGCGCAGGCGACGGCGAAAAGGGCCGTACCGAGTCGATTGACGTTCAGCGTGATGGCGGCGGCCGCGTTAC
CCTGCGCATCTTCGAAAGTCTTAAGCCCGTAATCGGCGCCATCAACGGCGCGGCTGTCGGTATCGGCGTGACGATGCAGC
TGCCGATGGATATTCGCATTGCGTCTGACAAGGCCCGCTTCGGCTTCGTGTTCAATCGCCGGGGCATTAACCCGGAAGCG
GCGTCCAGCTGGTTCCTGCCGCGCCTTGTCGGCATCCAGCAGGCGCTGGAATGGTGCTACACCGGCCGCGTCTTCCCGGC
GGATGAAGCCCTCAAGGGCGGCCTTGTGAGCAAAGTTGTTCCGCATGCAGACCTGATGAGCGAAGCGCGCAAACTTGCCC
GCGAGATCGCCGACAATACCGCGCCGGTTTCCAACGCACTGACCCGCCAGATGATGTGGCGCATGCTGGGCGCGAGCCAT
CCGATGGAAGCGCACATCGTGGATTCCGCGGCCATCTATACTCGTGGCAAAACACCAGATGCCAAAGAAGGCGTGATGAG
CTTCCTCGAGAAGCGCCAGCCGACCTATCCGGTCAAAGTGTCGGATGGCATGCCCGGCTTCTTCCCCTGGTGGGACGAGA
AGGAATTCGAGTGGATCGGCAACAAGTGA

Upstream 100 bases:

>100_bases
GTGGTGGGGGCTGCCGCGTCAAGCACCTCGGCGCGCTTGACGAACCTAAGGGAAGGAGGGCCTTCTCAGGCGGGAAACAC
ATCACTGGAGGAAATTGACG

Downstream 100 bases:

>100_bases
TTTCAGCGGCAAAGGGGCAGTAGGGCCATGCAGACTTTATTGCCTCTTGCCGCCGAGATTGGCGACATCCTGAAGGCGCG
GCGGGAAACCGTCGCGATTT

Product: enoyl-CoA hydratase/isomerase family protein

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MARPKEFKEIILDIEDGVATLTLHRPDNMNAFTGTMMYEMIEAFDITDANDDVKVVIVTGHGDRAFCAGADLSAGAKTFD
YDARAGDGEKGRTESIDVQRDGGGRVTLRIFESLKPVIGAINGAAVGIGVTMQLPMDIRIASDKARFGFVFNRRGINPEA
ASSWFLPRLVGIQQALEWCYTGRVFPADEALKGGLVSKVVPHADLMSEARKLAREIADNTAPVSNALTRQMMWRMLGASH
PMEAHIVDSAAIYTRGKTPDAKEGVMSFLEKRQPTYPVKVSDGMPGFFPWWDEKEFEWIGNK

Sequences:

>Translated_302_residues
MARPKEFKEIILDIEDGVATLTLHRPDNMNAFTGTMMYEMIEAFDITDANDDVKVVIVTGHGDRAFCAGADLSAGAKTFD
YDARAGDGEKGRTESIDVQRDGGGRVTLRIFESLKPVIGAINGAAVGIGVTMQLPMDIRIASDKARFGFVFNRRGINPEA
ASSWFLPRLVGIQQALEWCYTGRVFPADEALKGGLVSKVVPHADLMSEARKLAREIADNTAPVSNALTRQMMWRMLGASH
PMEAHIVDSAAIYTRGKTPDAKEGVMSFLEKRQPTYPVKVSDGMPGFFPWWDEKEFEWIGNK
>Mature_301_residues
ARPKEFKEIILDIEDGVATLTLHRPDNMNAFTGTMMYEMIEAFDITDANDDVKVVIVTGHGDRAFCAGADLSAGAKTFDY
DARAGDGEKGRTESIDVQRDGGGRVTLRIFESLKPVIGAINGAAVGIGVTMQLPMDIRIASDKARFGFVFNRRGINPEAA
SSWFLPRLVGIQQALEWCYTGRVFPADEALKGGLVSKVVPHADLMSEARKLAREIADNTAPVSNALTRQMMWRMLGASHP
MEAHIVDSAAIYTRGKTPDAKEGVMSFLEKRQPTYPVKVSDGMPGFFPWWDEKEFEWIGNK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=281, Percent_Identity=29.8932384341637, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI260274832, Length=269, Percent_Identity=26.3940520446097, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI45643119, Length=269, Percent_Identity=26.3940520446097, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI260275230, Length=269, Percent_Identity=26.3940520446097, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI194097323, Length=264, Percent_Identity=29.9242424242424, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI116235487, Length=234, Percent_Identity=29.4871794871795, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI68989263, Length=208, Percent_Identity=29.3269230769231, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI4757968, Length=247, Percent_Identity=27.1255060728745, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI49355787, Length=247, Percent_Identity=27.1255060728745, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI70995211, Length=216, Percent_Identity=30.5555555555556, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI4502327, Length=266, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI221136756, Length=245, Percent_Identity=27.3469387755102, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221136753, Length=245, Percent_Identity=27.3469387755102, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4757966, Length=212, Percent_Identity=26.4150943396226, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI51538581, Length=212, Percent_Identity=26.4150943396226, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI25453481, Length=212, Percent_Identity=26.4150943396226, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI51538573, Length=212, Percent_Identity=26.4150943396226, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI221307494, Length=245, Percent_Identity=27.3469387755102, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI37594471, Length=164, Percent_Identity=25, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI37594469, Length=164, Percent_Identity=25, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI20127408, Length=192, Percent_Identity=26.5625, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1788597, Length=261, Percent_Identity=30.6513409961686, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=32.5757575757576, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787660, Length=277, Percent_Identity=32.4909747292419, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787659, Length=271, Percent_Identity=28.0442804428044, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788682, Length=237, Percent_Identity=26.5822784810127, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790281, Length=202, Percent_Identity=28.7128712871287, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87082183, Length=269, Percent_Identity=23.4200743494424, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=268, Percent_Identity=30.9701492537313, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17549921, Length=223, Percent_Identity=30.4932735426009, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI25145438, Length=264, Percent_Identity=31.8181818181818, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17535521, Length=262, Percent_Identity=28.2442748091603, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=211, Percent_Identity=32.2274881516588, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17560910, Length=206, Percent_Identity=29.6116504854369, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=215, Percent_Identity=25.5813953488372, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=215, Percent_Identity=25.5813953488372, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=273, Percent_Identity=27.8388278388278, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=268, Percent_Identity=24.6268656716418, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=209, Percent_Identity=25.8373205741627, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI20129971, Length=268, Percent_Identity=31.7164179104478, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24653477, Length=268, Percent_Identity=31.7164179104478, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24653139, Length=269, Percent_Identity=31.2267657992565, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI19920382, Length=223, Percent_Identity=32.2869955156951, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21357171, Length=211, Percent_Identity=28.9099526066351, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24654903, Length=268, Percent_Identity=23.134328358209, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24650670, Length=211, Percent_Identity=28.9099526066351, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 33263; Mature: 33132

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPKEFKEIILDIEDGVATLTLHRPDNMNAFTGTMMYEMIEAFDITDANDDVKVVIVTG
CCCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
HGDRAFCAGADLSAGAKTFDYDARAGDGEKGRTESIDVQRDGGGRVTLRIFESLKPVIGA
CCCCEEEECCCCCCCCCEECCCCCCCCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHC
INGAAVGIGVTMQLPMDIRIASDKARFGFVFNRRGINPEAASSWFLPRLVGIQQALEWCY
CCCCEEEEEEEEECCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
TGRVFPADEALKGGLVSKVVPHADLMSEARKLAREIADNTAPVSNALTRQMMWRMLGASH
CCCCCCCHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
PMEAHIVDSAAIYTRGKTPDAKEGVMSFLEKRQPTYPVKVSDGMPGFFPWWDEKEFEWIG
CCEEEEECCEEEEECCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCC
NK
CC
>Mature Secondary Structure 
ARPKEFKEIILDIEDGVATLTLHRPDNMNAFTGTMMYEMIEAFDITDANDDVKVVIVTG
CCHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
HGDRAFCAGADLSAGAKTFDYDARAGDGEKGRTESIDVQRDGGGRVTLRIFESLKPVIGA
CCCCEEEECCCCCCCCCEECCCCCCCCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHC
INGAAVGIGVTMQLPMDIRIASDKARFGFVFNRRGINPEAASSWFLPRLVGIQQALEWCY
CCCCEEEEEEEEECCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
TGRVFPADEALKGGLVSKVVPHADLMSEARKLAREIADNTAPVSNALTRQMMWRMLGASH
CCCCCCCHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
PMEAHIVDSAAIYTRGKTPDAKEGVMSFLEKRQPTYPVKVSDGMPGFFPWWDEKEFEWIG
CCEEEEECCEEEEECCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCC
NK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA