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The map label for this gene is miaB

Identifier: 114799640

GI number: 114799640

Start: 91688

End: 93037

Strand: Direct

Name: miaB

Synonym: HNE_0103

Alternate gene names: 114799640

Gene position: 91688-93037 (Clockwise)

Preceding gene: 114797977

Following gene: 114800486

Centisome position: 2.47

GC content: 61.11

Gene sequence:

>1350_bases
ATGAAGGGCCTGTTTATCAGGACCTATGGCTGCCAGATGAATGTTTACGATTCCGAGCGCATCCGCGATGTGCTGCGGCC
GCTGGGCTATGCACCCGTGGAAACGCCGGAATCAGCCGACCTTGTCGTGGTCAACACCTGCCACATCCGCGAGAAAGCAA
CCGAGAAGGTCTACTCCGAACTCGGCCAGCTCAAACGGATGAAAGAAGCTTCCGGCGGGCGGATGACGATCGCGGTCGCC
GGCTGCGTCGCCCAGGCTGAAGGGGAGGAACTCATCCGCCGTCAGCCCGCGGTCGATCTGGTGCTTGGCCCGCAGGCCTA
TCACAAGCTGCCCGAGATGATCGCCCGCGCCAGCCGCGCCATCGGCGACCGGCTGGAAACCGAATTTGATACCGTCGAGA
AATTCGACGCCTTGCCCAAGACCCGCGAAGCCGATGGTCCGGCCGCTTTTGTTTCGGTGCAGGAAGGGTGTGACAAGTTC
TGCACCTTCTGTGTCGTGCCCTATACGCGCGGCGCTGAGATGTCCCGCCGCGTGGACGACATTGTGTTCGAGACCCGCAG
TCTGGCCAGCCAGGGCGTGCGCGAGATCACTTTGCTGGGTCAGAACGTAAATGCGTTTCACGGTCCTGCGCCTGTCCTGG
AGGGCGGGGAAGATTGGACGCTTGGCCAACTTTGTCGCCATTTGTCCAAAATTGGCGGCATTGAGCGCATCCGGTACACG
ACCAGTCATCCCCGCGACATGGATGACGATCTGATTGCCGCGCATGGCGACACGCCGGCCATGATGCCGTTCCTGCACTT
GCCGGTACAATCCGGGTCTGACCGCATTCTCAAGGCCATGAACCGCGGCCATACGGCCGATCACTACAGGGACATCATCA
CGCGGGTACGCGCCGCGCGGCCAGACATTGCCATCGCCTCTGATTTCATCGTCGGCTTTCCGGGCGAGAGTGACGCAGAT
TTTGAGGCGACGATGCAGCTCGTCCGCGACATCGGCTATGCCATTGCCTACTCGTTCAAATACTCCTCTCGCCCCGGCAC
GCCGGCGGCGGAAATGCACGGACATCTGTCCGAATCCGTAAAGGATGCGCGTCTTCAGGCGCTTCAGGCTTTGCTGCGCG
AACAGCAGACAGAGTTCAACGCCTCCCAGATCGGCAAAACCCTGCCCGTCCTCGTCACTGGCAAGGGCCGGAACGCGGGC
CAGATGCATGGCCGTTCGCCCTATCTGCAGGCGGTACACTTCGAAGGACCAGACGACCTGAACGGCAAGATTGTAGACGT
GAAAGTCATCGGCGCGAGCCTCAATTCTCTGACGGGTGAGCTGGTGCGTGTGGCTGAGGCCGCTCTGTGA

Upstream 100 bases:

>100_bases
CGGGCTTTGTTGCGGATGGTTCTTTCAGCTTGCCCGCTTTTGAGGCATAAGCCGCCAGATGACCGGCACCCCCGACCAAA
AGCCTCCCGGCGAGAAGCCC

Downstream 100 bases:

>100_bases
CTGGAAAGCGCCGAACGCCGTCCGAGCCCGAACCCCAAATGGTCAGCCGCCTCTATGAGGTGCGCGATGCCGAACGTCTG
CGCGATCTGTGCGGGCCGCA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MKGLFIRTYGCQMNVYDSERIRDVLRPLGYAPVETPESADLVVVNTCHIREKATEKVYSELGQLKRMKEASGGRMTIAVA
GCVAQAEGEELIRRQPAVDLVLGPQAYHKLPEMIARASRAIGDRLETEFDTVEKFDALPKTREADGPAAFVSVQEGCDKF
CTFCVVPYTRGAEMSRRVDDIVFETRSLASQGVREITLLGQNVNAFHGPAPVLEGGEDWTLGQLCRHLSKIGGIERIRYT
TSHPRDMDDDLIAAHGDTPAMMPFLHLPVQSGSDRILKAMNRGHTADHYRDIITRVRAARPDIAIASDFIVGFPGESDAD
FEATMQLVRDIGYAIAYSFKYSSRPGTPAAEMHGHLSESVKDARLQALQALLREQQTEFNASQIGKTLPVLVTGKGRNAG
QMHGRSPYLQAVHFEGPDDLNGKIVDVKVIGASLNSLTGELVRVAEAAL

Sequences:

>Translated_449_residues
MKGLFIRTYGCQMNVYDSERIRDVLRPLGYAPVETPESADLVVVNTCHIREKATEKVYSELGQLKRMKEASGGRMTIAVA
GCVAQAEGEELIRRQPAVDLVLGPQAYHKLPEMIARASRAIGDRLETEFDTVEKFDALPKTREADGPAAFVSVQEGCDKF
CTFCVVPYTRGAEMSRRVDDIVFETRSLASQGVREITLLGQNVNAFHGPAPVLEGGEDWTLGQLCRHLSKIGGIERIRYT
TSHPRDMDDDLIAAHGDTPAMMPFLHLPVQSGSDRILKAMNRGHTADHYRDIITRVRAARPDIAIASDFIVGFPGESDAD
FEATMQLVRDIGYAIAYSFKYSSRPGTPAAEMHGHLSESVKDARLQALQALLREQQTEFNASQIGKTLPVLVTGKGRNAG
QMHGRSPYLQAVHFEGPDDLNGKIVDVKVIGASLNSLTGELVRVAEAAL
>Mature_449_residues
MKGLFIRTYGCQMNVYDSERIRDVLRPLGYAPVETPESADLVVVNTCHIREKATEKVYSELGQLKRMKEASGGRMTIAVA
GCVAQAEGEELIRRQPAVDLVLGPQAYHKLPEMIARASRAIGDRLETEFDTVEKFDALPKTREADGPAAFVSVQEGCDKF
CTFCVVPYTRGAEMSRRVDDIVFETRSLASQGVREITLLGQNVNAFHGPAPVLEGGEDWTLGQLCRHLSKIGGIERIRYT
TSHPRDMDDDLIAAHGDTPAMMPFLHLPVQSGSDRILKAMNRGHTADHYRDIITRVRAARPDIAIASDFIVGFPGESDAD
FEATMQLVRDIGYAIAYSFKYSSRPGTPAAEMHGHLSESVKDARLQALQALLREQQTEFNASQIGKTLPVLVTGKGRNAG
QMHGRSPYLQAVHFEGPDDLNGKIVDVKVIGASLNSLTGELVRVAEAAL

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=480, Percent_Identity=33.9583333333333, Blast_Score=272, Evalue=4e-73,
Organism=Homo sapiens, GI28872782, Length=480, Percent_Identity=33.9583333333333, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI93277076, Length=457, Percent_Identity=26.2582056892779, Blast_Score=149, Evalue=5e-36,
Organism=Escherichia coli, GI1786882, Length=450, Percent_Identity=49.3333333333333, Blast_Score=430, Evalue=1e-122,
Organism=Escherichia coli, GI1787057, Length=449, Percent_Identity=28.0623608017817, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17553146, Length=423, Percent_Identity=34.7517730496454, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI71996771, Length=334, Percent_Identity=29.940119760479, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21356207, Length=450, Percent_Identity=32.2222222222222, Blast_Score=266, Evalue=1e-71,
Organism=Drosophila melanogaster, GI19922432, Length=464, Percent_Identity=27.1551724137931, Blast_Score=140, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_HYPNA (Q0C606)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_758837.1
- ProteinModelPortal:   Q0C606
- SMR:   Q0C606
- STRING:   Q0C606
- GeneID:   4290280
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0103
- NMPDR:   fig|228405.5.peg.101
- TIGR:   HNE_0103
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q0C606
- ProtClustDB:   PRK14325
- BioCyc:   HNEP81032:HNE_0103-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49264; Mature: 49264

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLFIRTYGCQMNVYDSERIRDVLRPLGYAPVETPESADLVVVNTCHIREKATEKVYSE
CCCEEEEEECCEEECCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHH
LGQLKRMKEASGGRMTIAVAGCVAQAEGEELIRRQPAVDLVLGPQAYHKLPEMIARASRA
HHHHHHHHHCCCCEEEEEEECHHHHCCHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHH
IGDRLETEFDTVEKFDALPKTREADGPAAFVSVQEGCDKFCTFCVVPYTRGAEMSRRVDD
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHH
IVFETRSLASQGVREITLLGQNVNAFHGPAPVLEGGEDWTLGQLCRHLSKIGGIERIRYT
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEC
TSHPRDMDDDLIAAHGDTPAMMPFLHLPVQSGSDRILKAMNRGHTADHYRDIITRVRAAR
CCCCCCCCCCEEEECCCCCCCCCEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCC
PDIAIASDFIVGFPGESDADFEATMQLVRDIGYAIAYSFKYSSRPGTPAAEMHGHLSESV
CCCEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHH
KDARLQALQALLREQQTEFNASQIGKTLPVLVTGKGRNAGQMHGRSPYLQAVHFEGPDDL
HHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCC
NGKIVDVKVIGASLNSLTGELVRVAEAAL
CCEEEEEEEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKGLFIRTYGCQMNVYDSERIRDVLRPLGYAPVETPESADLVVVNTCHIREKATEKVYSE
CCCEEEEEECCEEECCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHH
LGQLKRMKEASGGRMTIAVAGCVAQAEGEELIRRQPAVDLVLGPQAYHKLPEMIARASRA
HHHHHHHHHCCCCEEEEEEECHHHHCCHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHH
IGDRLETEFDTVEKFDALPKTREADGPAAFVSVQEGCDKFCTFCVVPYTRGAEMSRRVDD
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHH
IVFETRSLASQGVREITLLGQNVNAFHGPAPVLEGGEDWTLGQLCRHLSKIGGIERIRYT
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEC
TSHPRDMDDDLIAAHGDTPAMMPFLHLPVQSGSDRILKAMNRGHTADHYRDIITRVRAAR
CCCCCCCCCCEEEECCCCCCCCCEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCC
PDIAIASDFIVGFPGESDADFEATMQLVRDIGYAIAYSFKYSSRPGTPAAEMHGHLSESV
CCCEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHH
KDARLQALQALLREQQTEFNASQIGKTLPVLVTGKGRNAGQMHGRSPYLQAVHFEGPDDL
HHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCC
NGKIVDVKVIGASLNSLTGELVRVAEAAL
CCEEEEEEEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA