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The map label for this gene is nrdF [H]

Identifier: 114799612

GI number: 114799612

Start: 363300

End: 364337

Strand: Reverse

Name: nrdF [H]

Synonym: HNE_0373

Alternate gene names: 114799612

Gene position: 364337-363300 (Counterclockwise)

Preceding gene: 114800371

Following gene: 114798398

Centisome position: 9.83

GC content: 59.54

Gene sequence:

>1038_bases
ATGGCAAATGTAAGCACTGACCGCCCCGGCCTGCTGACGCCGAGCAATGCCTACAAGCCGTTCCGCTATCCGTGGGCCTA
TGAGTTCTGGAAGAAGCAGCAACAGGTTCACTGGATGCCCGAGGAAGTACCCCTCGGCGAAGACTGTAAAGACTGGGCGG
TGAAGCTCTCGGACGCCGAGCGCAACCTGCTGACCCAGATCTTCCGCTTCTTCACCCAGTCGGATGTCGAAGTCGGCGCC
AACTATATGGAACACTACATGCCGCTGTTTAAGCCGGTGGAAGTGTCCATGATGCTCGCCTCCTTCTCCAATATGGAGAC
GATCCATGTGGCGGCCTATGCCCTGCTGCTGGAAACCATCGGCATGCCGGATTCAGAATTCTCCGCCTTCATGGAATACA
AGGAGATGGCCGCAAAGCACGACTATCTCGGCCAGTTCGGCACCGAGACGAATGAAGACATCGCCGTCTCGATGGCCGTG
TTCGGCGCCTTCACCGAAGGGCTGCAGCTGTTTGCCTCCTTCGCGATGCTGATGAACTTCCCGCGCTTCAACAAGATGAA
GGGCATGGGCCAGATCGTCACCTGGTCGATCCGCGATGAGAGCCTCCACTGCGAAGGCATGATCAAGCTGTTCCATTCCT
TCTGCGCCGAAACCGGCGTCATGACGGATGAGCTGCGCGAGCGTATCCGCGACTGTTGCCGCACCGTGGTGGCGCTGGAA
GACAAGTTCATCGAACTGGCCTTCGAGATGGGCCCGGTCGAAGGCATGACGGCCGACGACATCAAGAACTACATCCGCTA
CATCGCCGACTGGCGCCTCAACCAGCTCAAGCTCGAGCCGATCTACGGCGTGACCAAACACCCGATCCCGTGGCTGACCG
AAATCCTCAACGGGGTCGAGCACGCCAACTTCTTCGAGCAGCGCTCCACCGAATATTCCAAGGGCGCCACCAAGGGCGAC
TGGCACGGCGACGATGGCGTCTGGGGCCGCTTCGACGCCCGCAAGAACGCTGGCACGGATACTGTTCCGGCGGAGTGA

Upstream 100 bases:

>100_bases
GCGGCGTTTACCTATTTTAAGCACTATATGTGGTGTGTTGAATGTCCCGAAGGCCAGATTCGCTGGCCCCGCAGACGCCT
GACTTGATGGAGCCCGACCC

Downstream 100 bases:

>100_bases
TCATGCTTCCAAACTCCTTCTCCCTTGGGAGAAGGAGTTTGTGACACTTAAGACGAGCTGCCGTTTAAAGCCCTTTGCCC
TTCGGGCCCTGCCCCGCTAA

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MANVSTDRPGLLTPSNAYKPFRYPWAYEFWKKQQQVHWMPEEVPLGEDCKDWAVKLSDAERNLLTQIFRFFTQSDVEVGA
NYMEHYMPLFKPVEVSMMLASFSNMETIHVAAYALLLETIGMPDSEFSAFMEYKEMAAKHDYLGQFGTETNEDIAVSMAV
FGAFTEGLQLFASFAMLMNFPRFNKMKGMGQIVTWSIRDESLHCEGMIKLFHSFCAETGVMTDELRERIRDCCRTVVALE
DKFIELAFEMGPVEGMTADDIKNYIRYIADWRLNQLKLEPIYGVTKHPIPWLTEILNGVEHANFFEQRSTEYSKGATKGD
WHGDDGVWGRFDARKNAGTDTVPAE

Sequences:

>Translated_345_residues
MANVSTDRPGLLTPSNAYKPFRYPWAYEFWKKQQQVHWMPEEVPLGEDCKDWAVKLSDAERNLLTQIFRFFTQSDVEVGA
NYMEHYMPLFKPVEVSMMLASFSNMETIHVAAYALLLETIGMPDSEFSAFMEYKEMAAKHDYLGQFGTETNEDIAVSMAV
FGAFTEGLQLFASFAMLMNFPRFNKMKGMGQIVTWSIRDESLHCEGMIKLFHSFCAETGVMTDELRERIRDCCRTVVALE
DKFIELAFEMGPVEGMTADDIKNYIRYIADWRLNQLKLEPIYGVTKHPIPWLTEILNGVEHANFFEQRSTEYSKGATKGD
WHGDDGVWGRFDARKNAGTDTVPAE
>Mature_344_residues
ANVSTDRPGLLTPSNAYKPFRYPWAYEFWKKQQQVHWMPEEVPLGEDCKDWAVKLSDAERNLLTQIFRFFTQSDVEVGAN
YMEHYMPLFKPVEVSMMLASFSNMETIHVAAYALLLETIGMPDSEFSAFMEYKEMAAKHDYLGQFGTETNEDIAVSMAVF
GAFTEGLQLFASFAMLMNFPRFNKMKGMGQIVTWSIRDESLHCEGMIKLFHSFCAETGVMTDELRERIRDCCRTVVALED
KFIELAFEMGPVEGMTADDIKNYIRYIADWRLNQLKLEPIYGVTKHPIPWLTEILNGVEHANFFEQRSTEYSKGATKGDW
HGDDGVWGRFDARKNAGTDTVPAE

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=323, Percent_Identity=26.3157894736842, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI289177074, Length=323, Percent_Identity=26.3157894736842, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI260064013, Length=300, Percent_Identity=26.3333333333333, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI4557845, Length=300, Percent_Identity=26.3333333333333, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI289177076, Length=285, Percent_Identity=25.9649122807018, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1788567, Length=312, Percent_Identity=23.0769230769231, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789031, Length=292, Percent_Identity=21.5753424657534, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17551968, Length=318, Percent_Identity=26.7295597484277, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI193206379, Length=273, Percent_Identity=24.9084249084249, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6322434, Length=303, Percent_Identity=24.4224422442244, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI18079279, Length=300, Percent_Identity=28.3333333333333, Blast_Score=105, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 39626; Mature: 39494

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVSTDRPGLLTPSNAYKPFRYPWAYEFWKKQQQVHWMPEEVPLGEDCKDWAVKLSDAE
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEHHHHH
RNLLTQIFRFFTQSDVEVGANYMEHYMPLFKPVEVSMMLASFSNMETIHVAAYALLLETI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GMPDSEFSAFMEYKEMAAKHDYLGQFGTETNEDIAVSMAVFGAFTEGLQLFASFAMLMNF
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PRFNKMKGMGQIVTWSIRDESLHCEGMIKLFHSFCAETGVMTDELRERIRDCCRTVVALE
CCHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
DKFIELAFEMGPVEGMTADDIKNYIRYIADWRLNQLKLEPIYGVTKHPIPWLTEILNGVE
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHH
HANFFEQRSTEYSKGATKGDWHGDDGVWGRFDARKNAGTDTVPAE
HHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ANVSTDRPGLLTPSNAYKPFRYPWAYEFWKKQQQVHWMPEEVPLGEDCKDWAVKLSDAE
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEHHHHH
RNLLTQIFRFFTQSDVEVGANYMEHYMPLFKPVEVSMMLASFSNMETIHVAAYALLLETI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GMPDSEFSAFMEYKEMAAKHDYLGQFGTETNEDIAVSMAVFGAFTEGLQLFASFAMLMNF
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PRFNKMKGMGQIVTWSIRDESLHCEGMIKLFHSFCAETGVMTDELRERIRDCCRTVVALE
CCHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
DKFIELAFEMGPVEGMTADDIKNYIRYIADWRLNQLKLEPIYGVTKHPIPWLTEILNGVE
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHH
HANFFEQRSTEYSKGATKGDWHGDDGVWGRFDARKNAGTDTVPAE
HHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]