Click here to switch to the map view.

The map label for this gene is kstD [H]

Identifier: 114799458

GI number: 114799458

Start: 1478338

End: 1479855

Strand: Reverse

Name: kstD [H]

Synonym: HNE_1451

Alternate gene names: 114799458

Gene position: 1479855-1478338 (Counterclockwise)

Preceding gene: 114797904

Following gene: 114800251

Centisome position: 39.94

GC content: 59.42

Gene sequence:

>1518_bases
ATGGCCAGCCTTAAAAGTGTCAGCTCCTGGAGCGAAGAAGTAGATGTTGTCATCTGTGGATACGGATGTGCCGGCGCTTC
TGCGGCGATTGAAGCCTACGACACGGACCCTTCTGCACGCCTCCTGATAATTGAAAAGGCGCCGCAGGAATACGCAGGGG
GCAATTGCCGCGTCTCCGGCCAGTCGCTCCTTATCTCGAAGAATAAAACTGCCCTGAAAGACTATCAGCGGGCCATGAGC
ACAGCCAATCCGATCCCTGAGGATATTCTGGAGGACTGGGCAGACCGTATGGTCAATCTTGAACCCTGGATTCAGAAGAT
TGCCAGAGACGCTGATGCTGAATTTCTGCGGGGGACCGGATTTACAGAACGTGACGCCGTTCTGGAGTTTCCGGAAATGG
GCGCGCGCGAGGCCGTCGCCTACACCTCAACCATTCTTCCCATTCCCTCTGGTGTCTGGCTGGCCCTGCAAGCCAATGTT
GAAAAGCGCGGGGTGCCCGTCTGGTTTGACGCGCCGCTGGTTGATCTCATACAGGACCCTGACACGCTGGAAGTGTTTGG
TGTCTGGGTGGAGAAGGACGGCGTCCGCCAGGCGATCCGCGCGCGCAAGGCGGTGATCCTCTCCGTAGGCGGATATGAAG
CGGACCCTGAGATGCAGAAAAACTATTATGGCCTGTCCGATGTCTGCCCCCTGGGCACACCCTACAACACAGGCGACGGC
GTGCGCATTCTGCAGAAAGCGGGCGCCGAGATGTGGCACCTGCGCAATCAGGGCCAGTCGGGCGGTATCTGGCCGGGCAT
CAAGCGGCCAGATGATGAAACGTCGTTCCTGCGCAACTTCCTTCTGCCCGCCTTCTCCTGGATCGATGTGGACAGGACGG
GGCGGCGCTTTTATGGCGAAACCAATGAACTTCAGCTTACCCACTACAAGCACAGGAAGCATGGCCGCTGGGTCGATGTG
CCGCTGCACACCGCTCACCCCGTACACATGATCTTCGACGAAACGACACGGCGGGCGGGAAAGCTCGTGCTGGACGTGAT
GACCTGGAGCGCCGTGGTCAAAAAATATCCGTGGAGTGAGGATAACAGCGACGAGGTTGCTTCGGGGCTTATCAGGAAAG
CGGACAGCATCGAAGCGCTTGCCGGGCTAATTGGTCTGAGCCCGGAAATACTCTCGGCGGAGGTTTCTGCCTATAACGCT
GCCTGCGAGGCGGGCGAGGATGCTGAACAAGGGCGCAACCCTGAAACGCTCATGCCCATCGCCCAAGGTCCCTTTTATGC
CGTTCAGCTCACCCCGGCCATTGTCTGTACCGGGGGCGGCGCACGGCGCGATATGGATGGCCGGGTTTACGATCACAAGG
GCTCGGCCATTCCGCGCCTGTTCGAGGCGGGCGAGCTGGGTTCCATCTTCTCTGATCTCTACCAGAACGGCTCCTATCTG
ACCGAAGCCATGATTACGGGCCGGGGCGCGGCACGCGCTGCGCTTGCCACGGTGCCCGTCAAAACTGCGGCGGAATAG

Upstream 100 bases:

>100_bases
CTCCATGACATTAACAAACACAGATTGTGCATATGGACAACTGATTGTTAGATCGTGTGAGTAATCCATGGCCGAATGAT
CTCGTCAGAGGAGACGGATC

Downstream 100 bases:

>100_bases
TCCTCATGCTGGTCAAGACTCCCGCCCTCAAGCTGGGCGTTGAAGTGAGAGAAACGTTTGTGGAGAATGACCGCCTCATT
CTCAAAGGGGTCGCCAACGC

Product: FAD-binding domain-containing protein

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MASLKSVSSWSEEVDVVICGYGCAGASAAIEAYDTDPSARLLIIEKAPQEYAGGNCRVSGQSLLISKNKTALKDYQRAMS
TANPIPEDILEDWADRMVNLEPWIQKIARDADAEFLRGTGFTERDAVLEFPEMGAREAVAYTSTILPIPSGVWLALQANV
EKRGVPVWFDAPLVDLIQDPDTLEVFGVWVEKDGVRQAIRARKAVILSVGGYEADPEMQKNYYGLSDVCPLGTPYNTGDG
VRILQKAGAEMWHLRNQGQSGGIWPGIKRPDDETSFLRNFLLPAFSWIDVDRTGRRFYGETNELQLTHYKHRKHGRWVDV
PLHTAHPVHMIFDETTRRAGKLVLDVMTWSAVVKKYPWSEDNSDEVASGLIRKADSIEALAGLIGLSPEILSAEVSAYNA
ACEAGEDAEQGRNPETLMPIAQGPFYAVQLTPAIVCTGGGARRDMDGRVYDHKGSAIPRLFEAGELGSIFSDLYQNGSYL
TEAMITGRGAARAALATVPVKTAAE

Sequences:

>Translated_505_residues
MASLKSVSSWSEEVDVVICGYGCAGASAAIEAYDTDPSARLLIIEKAPQEYAGGNCRVSGQSLLISKNKTALKDYQRAMS
TANPIPEDILEDWADRMVNLEPWIQKIARDADAEFLRGTGFTERDAVLEFPEMGAREAVAYTSTILPIPSGVWLALQANV
EKRGVPVWFDAPLVDLIQDPDTLEVFGVWVEKDGVRQAIRARKAVILSVGGYEADPEMQKNYYGLSDVCPLGTPYNTGDG
VRILQKAGAEMWHLRNQGQSGGIWPGIKRPDDETSFLRNFLLPAFSWIDVDRTGRRFYGETNELQLTHYKHRKHGRWVDV
PLHTAHPVHMIFDETTRRAGKLVLDVMTWSAVVKKYPWSEDNSDEVASGLIRKADSIEALAGLIGLSPEILSAEVSAYNA
ACEAGEDAEQGRNPETLMPIAQGPFYAVQLTPAIVCTGGGARRDMDGRVYDHKGSAIPRLFEAGELGSIFSDLYQNGSYL
TEAMITGRGAARAALATVPVKTAAE
>Mature_504_residues
ASLKSVSSWSEEVDVVICGYGCAGASAAIEAYDTDPSARLLIIEKAPQEYAGGNCRVSGQSLLISKNKTALKDYQRAMST
ANPIPEDILEDWADRMVNLEPWIQKIARDADAEFLRGTGFTERDAVLEFPEMGAREAVAYTSTILPIPSGVWLALQANVE
KRGVPVWFDAPLVDLIQDPDTLEVFGVWVEKDGVRQAIRARKAVILSVGGYEADPEMQKNYYGLSDVCPLGTPYNTGDGV
RILQKAGAEMWHLRNQGQSGGIWPGIKRPDDETSFLRNFLLPAFSWIDVDRTGRRFYGETNELQLTHYKHRKHGRWVDVP
LHTAHPVHMIFDETTRRAGKLVLDVMTWSAVVKKYPWSEDNSDEVASGLIRKADSIEALAGLIGLSPEILSAEVSAYNAA
CEAGEDAEQGRNPETLMPIAQGPFYAVQLTPAIVCTGGGARRDMDGRVYDHKGSAIPRLFEAGELGSIFSDLYQNGSYLT
EAMITGRGAARAALATVPVKTAAE

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

Organism=Caenorhabditis elegans, GI71986328, Length=309, Percent_Identity=24.9190938511327, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 55299; Mature: 55168

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLKSVSSWSEEVDVVICGYGCAGASAAIEAYDTDPSARLLIIEKAPQEYAGGNCRVSG
CCCCHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCCCEEEEEECCCHHHCCCCEEECC
QSLLISKNKTALKDYQRAMSTANPIPEDILEDWADRMVNLEPWIQKIARDADAEFLRGTG
CEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCC
FTERDAVLEFPEMGAREAVAYTSTILPIPSGVWLALQANVEKRGVPVWFDAPLVDLIQDP
CCCCCCEECCCCCCCHHHHHHHEEEEECCCCEEEEEEECHHHCCCCEEECCCHHHHHCCC
DTLEVFGVWVEKDGVRQAIRARKAVILSVGGYEADPEMQKNYYGLSDVCPLGTPYNTGDG
CCEEEEEEEECCHHHHHHHHHCCEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCCC
VRILQKAGAEMWHLRNQGQSGGIWPGIKRPDDETSFLRNFLLPAFSWIDVDRTGRRFYGE
HHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCHHHCCC
TNELQLTHYKHRKHGRWVDVPLHTAHPVHMIFDETTRRAGKLVLDVMTWSAVVKKYPWSE
CCCEEEEEEHHHCCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DNSDEVASGLIRKADSIEALAGLIGLSPEILSAEVSAYNAACEAGEDAEQGRNPETLMPI
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEE
AQGPFYAVQLTPAIVCTGGGARRDMDGRVYDHKGSAIPRLFEAGELGSIFSDLYQNGSYL
CCCCEEEEEECCEEEECCCCCCCCCCCCEEECCCCCCHHHHHCCCHHHHHHHHHCCCCCE
TEAMITGRGAARAALATVPVKTAAE
EHHHHHCCCHHHHHHEECCCHHCCC
>Mature Secondary Structure 
ASLKSVSSWSEEVDVVICGYGCAGASAAIEAYDTDPSARLLIIEKAPQEYAGGNCRVSG
CCCHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCCCEEEEEECCCHHHCCCCEEECC
QSLLISKNKTALKDYQRAMSTANPIPEDILEDWADRMVNLEPWIQKIARDADAEFLRGTG
CEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHCCC
FTERDAVLEFPEMGAREAVAYTSTILPIPSGVWLALQANVEKRGVPVWFDAPLVDLIQDP
CCCCCCEECCCCCCCHHHHHHHEEEEECCCCEEEEEEECHHHCCCCEEECCCHHHHHCCC
DTLEVFGVWVEKDGVRQAIRARKAVILSVGGYEADPEMQKNYYGLSDVCPLGTPYNTGDG
CCEEEEEEEECCHHHHHHHHHCCEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCCC
VRILQKAGAEMWHLRNQGQSGGIWPGIKRPDDETSFLRNFLLPAFSWIDVDRTGRRFYGE
HHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCHHHCCC
TNELQLTHYKHRKHGRWVDVPLHTAHPVHMIFDETTRRAGKLVLDVMTWSAVVKKYPWSE
CCCEEEEEEHHHCCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DNSDEVASGLIRKADSIEALAGLIGLSPEILSAEVSAYNAACEAGEDAEQGRNPETLMPI
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEE
AQGPFYAVQLTPAIVCTGGGARRDMDGRVYDHKGSAIPRLFEAGELGSIFSDLYQNGSYL
CCCCEEEEEECCEEEECCCCCCCCCCCCEEECCCCCCHHHHHCCCHHHHHHHHHCCCCCE
TEAMITGRGAARAALATVPVKTAAE
EHHHHHCCCHHHHHHEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]