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The map label for this gene is ysgA [H]

Identifier: 114799377

GI number: 114799377

Start: 3026220

End: 3027035

Strand: Direct

Name: ysgA [H]

Synonym: HNE_2870

Alternate gene names: 114799377

Gene position: 3026220-3027035 (Clockwise)

Preceding gene: 114798598

Following gene: 114800149

Centisome position: 81.68

GC content: 63.11

Gene sequence:

>816_bases
ATGAAACAGCCCTTAGAACTGATTACCAGCCCCTCCAATCCGCTGATCAAGGCCCTGAAAAGCCTCGAGCGCAAGAAGGA
GCGCGCCGAAACCGGCCTGTTCCTCGCAGAAGGCGCACGGATCGTCAGCGAAGGCCTGGCGCGTGGCTGGCAGCCCGAAA
CCGTCATTATTGCCGCCGATATGGCAGAACGGCCACAGATCAGTGACTTGGCCAGAGCCGCTGCAAAGGCGGGCGCGCGC
GTTGTGATGGCGCCCGAGCGCCTGATGACCAAGATCACCCAGAAGGACAACGCGCAGAGCGTGGTCGCCGCCTTCCGCCA
GCGCCACCTCGGTGTTGAAAGCCTACCCAAACCGGCGGGCACGCCGCTCTACATCGCCCTCTATGAAGTGCGCGATCCCG
GCAATCTGGGCACCATCCTGCGCACCGCCGACTGCGCCGGGGTGAGCGGCGTCATCCTCATCGGAACCTGCTGTGATCCC
TACAGTTTTGAGGCCGTGCGGGCGTCGATGGGATCGGTGTTTGATGTGCCTTTTGCCCATACTGATTTTGACGCCTTCAA
TACCTGGCGCAAAGGCGAAGGCATGAGCCTCACAGCTGCCTCCGTAAACGGGACTGTGCGCCATGACGGGGCTGATTTCA
CCGGCGGATCGATCATCCTGATGGGTAATGAGCAGGCGGGCCTGCCCGACTGGGTGGAGGCGGCCTGCGACACCCTCTCC
CTCATTCCCATGCGCGGAGGCGCCGACAGCCTCAACCTCGCCCAGGCCAGCGCCCTGATGATCTATGAGGCCTGGCGCCA
GCGGGGGTATGCATGA

Upstream 100 bases:

>100_bases
CTGACCTCCCAAAACCAGTACCCCCACAGCACCCTCTCGGGCGAGGCATGCCCTAACCCTTACCAGACCGCAAGATGAAC
CAGGCAGGCTCCCTCCGGCG

Downstream 100 bases:

>100_bases
GCCTGAAAACCGAAACCCGCCTGCTGATTGCCGACGACTGGGAAGATTACGCCCTGCTCGACTCCGGCCACCTGCAGAAG
CTCGAACGCTTCGGCAGCCA

Product: RNA methyltransferase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKQPLELITSPSNPLIKALKSLERKKERAETGLFLAEGARIVSEGLARGWQPETVIIAADMAERPQISDLARAAAKAGAR
VVMAPERLMTKITQKDNAQSVVAAFRQRHLGVESLPKPAGTPLYIALYEVRDPGNLGTILRTADCAGVSGVILIGTCCDP
YSFEAVRASMGSVFDVPFAHTDFDAFNTWRKGEGMSLTAASVNGTVRHDGADFTGGSIILMGNEQAGLPDWVEAACDTLS
LIPMRGGADSLNLAQASALMIYEAWRQRGYA

Sequences:

>Translated_271_residues
MKQPLELITSPSNPLIKALKSLERKKERAETGLFLAEGARIVSEGLARGWQPETVIIAADMAERPQISDLARAAAKAGAR
VVMAPERLMTKITQKDNAQSVVAAFRQRHLGVESLPKPAGTPLYIALYEVRDPGNLGTILRTADCAGVSGVILIGTCCDP
YSFEAVRASMGSVFDVPFAHTDFDAFNTWRKGEGMSLTAASVNGTVRHDGADFTGGSIILMGNEQAGLPDWVEAACDTLS
LIPMRGGADSLNLAQASALMIYEAWRQRGYA
>Mature_271_residues
MKQPLELITSPSNPLIKALKSLERKKERAETGLFLAEGARIVSEGLARGWQPETVIIAADMAERPQISDLARAAAKAGAR
VVMAPERLMTKITQKDNAQSVVAAFRQRHLGVESLPKPAGTPLYIALYEVRDPGNLGTILRTADCAGVSGVILIGTCCDP
YSFEAVRASMGSVFDVPFAHTDFDAFNTWRKGEGMSLTAASVNGTVRHDGADFTGGSIILMGNEQAGLPDWVEAACDTLS
LIPMRGGADSLNLAQASALMIYEAWRQRGYA

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=150, Percent_Identity=34.6666666666667, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790623, Length=257, Percent_Identity=26.8482490272374, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24666840, Length=291, Percent_Identity=22.3367697594502, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 29023; Mature: 29023

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQPLELITSPSNPLIKALKSLERKKERAETGLFLAEGARIVSEGLARGWQPETVIIAAD
CCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCEEHHHHHHHHHHHHHCCCCCCEEEEECC
MAERPQISDLARAAAKAGARVVMAPERLMTKITQKDNAQSVVAAFRQRHLGVESLPKPAG
CCCCCCHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCCCC
TPLYIALYEVRDPGNLGTILRTADCAGVSGVILIGTCCDPYSFEAVRASMGSVFDVPFAH
CCEEEEEEEECCCCCCCHHEEECCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEECCCCC
TDFDAFNTWRKGEGMSLTAASVNGTVRHDGADFTGGSIILMGNEQAGLPDWVEAACDTLS
CCCHHHHCCCCCCCCEEEEEECCCEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHEE
LIPMRGGADSLNLAQASALMIYEAWRQRGYA
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKQPLELITSPSNPLIKALKSLERKKERAETGLFLAEGARIVSEGLARGWQPETVIIAAD
CCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCEEHHHHHHHHHHHHHCCCCCCEEEEECC
MAERPQISDLARAAAKAGARVVMAPERLMTKITQKDNAQSVVAAFRQRHLGVESLPKPAG
CCCCCCHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCCCC
TPLYIALYEVRDPGNLGTILRTADCAGVSGVILIGTCCDPYSFEAVRASMGSVFDVPFAH
CCEEEEEEEECCCCCCCHHEEECCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEECCCCC
TDFDAFNTWRKGEGMSLTAASVNGTVRHDGADFTGGSIILMGNEQAGLPDWVEAACDTLS
CCCHHHHCCCCCCCCEEEEEECCCEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHEE
LIPMRGGADSLNLAQASALMIYEAWRQRGYA
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]