Click here to switch to the map view.

The map label for this gene is gcvH [H]

Identifier: 114799251

GI number: 114799251

Start: 2883370

End: 2883741

Strand: Direct

Name: gcvH [H]

Synonym: HNE_2720

Alternate gene names: 114799251

Gene position: 2883370-2883741 (Clockwise)

Preceding gene: 114799925

Following gene: 114797821

Centisome position: 77.82

GC content: 58.87

Gene sequence:

>372_bases
ATGACCACCTACTACACCAAAGATCATGAATGGGTTCGCGTCGAAGGCGACACCGGCACTGTGGGCATCACCTCCTACGC
CGCCGGACAGCTCGGCGATGTTGTCTATGTTGAGCTTCCTGAAGCCGGCGCGAGCCTTGCCAAGGGCGACGGCTTTGCCG
TTGTCGAAAGCGTGAAAGCTGCTTCGGACGTGTATGCGCCAATTTCCGGCGCCGTTCTGGAATCCAATACCGGTCTTGCC
GACGCGCCTGAAAAGGTCAGCGAAGCGGCCGAGAGCGATGCCTGGTTCGTCCGCGTCAAGATTTCGGACACTGCCGAGCT
TGAAGGCCTGATGGATCTGGCCGCCTACACTGAATATTGCGAAGGGCTCTGA

Upstream 100 bases:

>100_bases
GATCGACCTTATCGTCCGCGGAAAGGCCCGGCCCGCCGAAGTGGCCGCCCTCCCGTTCGTTCCACAAAACTATAAACGCT
AGACCTGCAGGGGCACGCAC

Downstream 100 bases:

>100_bases
TTACATGCGCTATCTGCCGCTCACGCCTGAGGACCGCGCCAACATGCTGGCGACCATCGGCGCGAAATCCGTCGATGATT
TTTACACAGACGTTCCAGAC

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 123; Mature: 122

Protein sequence:

>123_residues
MTTYYTKDHEWVRVEGDTGTVGITSYAAGQLGDVVYVELPEAGASLAKGDGFAVVESVKAASDVYAPISGAVLESNTGLA
DAPEKVSEAAESDAWFVRVKISDTAELEGLMDLAAYTEYCEGL

Sequences:

>Translated_123_residues
MTTYYTKDHEWVRVEGDTGTVGITSYAAGQLGDVVYVELPEAGASLAKGDGFAVVESVKAASDVYAPISGAVLESNTGLA
DAPEKVSEAAESDAWFVRVKISDTAELEGLMDLAAYTEYCEGL
>Mature_122_residues
TTYYTKDHEWVRVEGDTGTVGITSYAAGQLGDVVYVELPEAGASLAKGDGFAVVESVKAASDVYAPISGAVLESNTGLAD
APEKVSEAAESDAWFVRVKISDTAELEGLMDLAAYTEYCEGL

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=45.3781512605042, Blast_Score=114, Evalue=2e-26,
Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=45.3781512605042, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789271, Length=118, Percent_Identity=50.8474576271186, Blast_Score=118, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=48.695652173913, Blast_Score=122, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=46.9565217391304, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319272, Length=116, Percent_Identity=44.8275862068966, Blast_Score=102, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17865652, Length=116, Percent_Identity=43.9655172413793, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 12934; Mature: 12803

Theoretical pI: Translated: 3.80; Mature: 3.80

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTYYTKDHEWVRVEGDTGTVGITSYAAGQLGDVVYVELPEAGASLAKGDGFAVVESVKA
CCCEECCCCCEEEEECCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHH
ASDVYAPISGAVLESNTGLADAPEKVSEAAESDAWFVRVKISDTAELEGLMDLAAYTEYC
HHHCCCCCCCEEEECCCCCCCCCHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHH
EGL
CCC
>Mature Secondary Structure 
TTYYTKDHEWVRVEGDTGTVGITSYAAGQLGDVVYVELPEAGASLAKGDGFAVVESVKA
CCEECCCCCEEEEECCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHH
ASDVYAPISGAVLESNTGLADAPEKVSEAAESDAWFVRVKISDTAELEGLMDLAAYTEYC
HHHCCCCCCCEEEECCCCCCCCCHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHH
EGL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA